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OPENSEQ.org
PTGCB - PTS system glucose-specific EIICB component
UniProt: P69786 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10787
Length: 477 (474)
Sequences: 1042
Seq/Len: 2.20
PTGCB
Paralog alert: 0.78 [within 20: 0.03] - ratio of genomes with paralogs
Cluster includes: PTGCB PTOCB PTW3C PTXC
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
263_A 365_A 4.774 1.00
270_H 373_R 4.575 1.00
373_R 377_K 3.785 1.00
270_H 369_Y 3.642 1.00
437_Q 450_V 3.309 1.00
65_I 83_V 3.03 1.00
85_A 157_S 2.977 1.00
416_T 430_A 2.889 1.00
268_I 284_M 2.844 1.00
27_G 336_S 2.843 1.00
382_K 388_D 2.782 1.00
313_I 317_I 2.711 1.00
362_I 366_I 2.697 1.00
418_L 474_I 2.669 1.00
271_S 377_K 2.609 1.00
271_S 373_R 2.585 1.00
265_A 300_I 2.557 1.00
358_P 362_I 2.539 1.00
53_G 57_A 2.458 1.00
318_L 371_I 2.402 1.00
202_E 253_G 2.371 1.00
259_Y 361_G 2.332 1.00
276_N 279_K 2.329 1.00
420_A 463_S 2.321 1.00
314_I 318_L 2.313 1.00
138_I 152_F 2.285 1.00
273_K 388_D 2.28 1.00
191_P 194_A 2.234 1.00
127_I 164_T 2.23 1.00
315_H 368_Y 2.211 1.00
270_H 377_K 2.051 0.99
367_V 371_I 2.047 0.99
258_M 292_F 2.042 0.99
449_V 458_I 1.995 0.99
156_I 160_A 1.952 0.99
459_F 463_S 1.92 0.99
329_L 360_V 1.918 0.99
138_I 148_A 1.909 0.99
330_G 353_K 1.903 0.99
431_D 434_K 1.899 0.99
15_S 71_F 1.896 0.99
14_K 419_D 1.882 0.99
406_V 474_I 1.863 0.98
199_G 323_F 1.854 0.98
436_D 439_G 1.842 0.98
375_L 379_L 1.812 0.98
413_E 434_K 1.774 0.98
406_V 470_M 1.767 0.98
126_A 130_Y 1.72 0.97
128_A 157_S 1.69 0.97
166_V 170_F 1.688 0.97
362_I 365_A 1.681 0.97
135_F 156_I 1.674 0.96
356_L 359_I 1.662 0.96
314_I 371_I 1.662 0.96
408_A 443_L 1.649 0.96
17_M 212_H 1.625 0.96
276_N 389_A 1.617 0.95
359_I 363_G 1.614 0.95
406_V 473_Y 1.601 0.95
201_I 214_W 1.59 0.95
445_A 448_V 1.587 0.95
124_S 164_T 1.585 0.95
324_P 328_L 1.583 0.95
310_I 314_I 1.569 0.94
25_I 158_G 1.566 0.94
269_W 277_R 1.564 0.94
269_W 281_G 1.56 0.94
131_M 160_A 1.552 0.94
420_A 443_L 1.55 0.94
282_G 300_I 1.544 0.94
261_L 264_A 1.531 0.93
131_M 156_I 1.52 0.93
405_L 459_F 1.509 0.93
200_F 316_A 1.5 0.92
314_I 375_L 1.499 0.92
281_G 285_I 1.489 0.92
341_L 345_I 1.488 0.92
417_N 428_S 1.486 0.92
266_I 270_H 1.465 0.91
326_C 331_M 1.454 0.91
256_F 319_A 1.436 0.90
408_A 439_G 1.436 0.90
159_L 163_F 1.431 0.90
283_I 287_A 1.413 0.89
374_V 378_A 1.405 0.89
30_L 56_F 1.404 0.89
418_L 470_M 1.399 0.88
429_V 432_V 1.389 0.88
443_L 463_S 1.387 0.88
371_I 375_L 1.385 0.88
383_T 386_R 1.372 0.87
124_S 161_A 1.365 0.87
200_F 204_C 1.36 0.86
127_I 160_A 1.358 0.86
439_G 442_K 1.35 0.86
139_K 142_E 1.338 0.85
406_V 418_L 1.336 0.85
409_F 457_A 1.33 0.85
145_G 296_I 1.329 0.85
196_G 200_F 1.328 0.85
33_G 52_G 1.327 0.85
253_G 335_T 1.317 0.84
202_E 335_T 1.308 0.83
131_M 152_F 1.303 0.83
200_F 313_I 1.298 0.83
9_L 12_V 1.289 0.82
255_L 322_A 1.286 0.82
160_A 164_T 1.286 0.82
266_I 365_A 1.277 0.81
427_V 470_M 1.275 0.81
344_F 355_W 1.273 0.81
429_V 435_V 1.269 0.81
278_A 281_G 1.267 0.81
273_K 387_E 1.265 0.81
239_R 244_D 1.26 0.80
224_E 228_A 1.257 0.80
467_K 471_D 1.253 0.80
202_E 319_A 1.248 0.79
61_L 83_V 1.244 0.79
237_I 250_L 1.241 0.79
193_V 197_I 1.231 0.78
272_A 383_T 1.218 0.77
261_L 315_H 1.216 0.77
430_A 434_K 1.215 0.77
274_P 277_R 1.213 0.77
376_I 383_T 1.21 0.76
409_F 470_M 1.21 0.76
407_A 439_G 1.21 0.76
422_I 463_S 1.204 0.76
138_I 156_I 1.2 0.75
192_V 328_L 1.193 0.75
326_C 360_V 1.193 0.75
293_L 342_I 1.189 0.75
38_S 41_P 1.188 0.74
416_T 428_S 1.181 0.74
273_K 382_K 1.179 0.74
220_M 237_I 1.177 0.73
280_V 284_M 1.172 0.73
457_A 463_S 1.17 0.73
401_M 462_K 1.17 0.73
215_N 237_I 1.162 0.72
18_L 69_L 1.162 0.72
437_Q 441_K 1.158 0.72
163_F 167_V 1.158 0.72
59_M 118_V 1.156 0.72
233_F 242_A 1.152 0.71
269_W 284_M 1.15 0.71
110_S 347_L 1.15 0.71
28_I 32_V 1.147 0.71
270_H 370_T 1.138 0.70
85_A 124_S 1.137 0.70
369_Y 377_K 1.137 0.70
374_V 379_L 1.137 0.70
329_L 356_L 1.131 0.69
135_F 138_I 1.12 0.68
440_L 457_A 1.12 0.68
366_I 370_T 1.12 0.68
376_I 386_R 1.115 0.68
219_Q 338_S 1.114 0.67
448_V 457_A 1.113 0.67
333_D 357_F 1.102 0.66
435_V 455_V 1.099 0.66
463_S 470_M 1.095 0.66
204_C 316_A 1.091 0.65
310_I 379_L 1.089 0.65
432_V 455_V 1.089 0.65
46_H 50_E 1.088 0.65
152_F 156_I 1.081 0.64
192_V 324_P 1.077 0.64
271_S 369_Y 1.066 0.62
225_Y 228_A 1.063 0.62
404_A 443_L 1.063 0.62
344_F 348_S 1.061 0.62
457_A 466_L 1.059 0.62
261_L 300_I 1.054 0.61
432_V 453_S 1.053 0.61
463_S 466_L 1.051 0.61
236_D 248_G 1.049 0.61
92_T 165_G 1.048 0.61
166_V 171_I 1.046 0.60
420_A 466_L 1.044 0.60
16_L 150_K 1.04 0.60
69_L 79_A 1.039 0.60
255_L 326_C 1.037 0.59
428_S 454_G 1.035 0.59
246_T 249_K 1.031 0.59
264_A 300_I 1.03 0.59
331_M 356_L 1.029 0.58
71_F 126_A 1.029 0.58
355_W 359_I 1.029 0.58
402_A 473_Y 1.027 0.58
124_S 128_A 1.027 0.58
135_F 153_V 1.025 0.58
167_V 170_F 1.023 0.58
238_P 241_M 1.022 0.58
128_A 161_A 1.021 0.58
370_T 374_V 1.021 0.58
225_Y 239_R 1.02 0.57
268_I 271_S 1.018 0.57
376_I 385_G 1.018 0.57
294_T 340_G 1.015 0.57
265_A 284_M 1.013 0.57
256_F 364_Y 1.01 0.56
276_N 387_E 1.01 0.56
192_V 327_I 1.009 0.56
10_Q 13_G 1.007 0.56
321_L 325_I 1.004 0.56
395_A 399_S 1.002 0.55
195_F 200_F 1.002 0.55
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3qnqA40.8051000.733Contact Map0.476
1ibaA10.190899.90.85Contact Map0.221
3bp3A20.171999.90.85Contact Map0.719
3ipjA20.17499.90.863Contact Map0.682
3pcoA20.138415.40.983Contact Map0.06
3clmA10.13847.90.985Contact Map0.137
1b7yA10.14266.10.986Contact Map
2kncA10.10695.30.986Contact Map0.569
4c5pA10.13635.30.986Contact Map0.203
2h1vA10.16145.20.986Contact Map0.015
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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