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OPENSEQ.org
PTOCB - PTS system maltose- and glucose-specific EIICB component
UniProt: P19642 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10563
Length: 530 (518)
Sequences: 958
Seq/Len: 1.85
PTOCB
Paralog alert: 0.76 [within 20: 0.02] - ratio of genomes with paralogs
Cluster includes: PTGCB PTOCB PTW3C PTXC
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
304_G 408_F 4.839 1.00
311_H 416_R 4.314 1.00
311_H 412_Y 3.844 1.00
416_R 420_T 3.531 1.00
487_V 500_Q 3.349 1.00
83_I 102_F 3.34 1.00
104_G 187_S 3.335 1.00
468_L 525_M 2.973 1.00
466_V 480_K 2.789 1.00
470_N 514_V 2.698 1.00
309_M 325_L 2.68 1.00
306_A 341_L 2.655 1.00
425_K 431_S 2.609 1.00
231_E 294_K 2.555 1.00
300_G 404_A 2.495 1.00
356_H 411_Y 2.477 1.00
401_P 405_A 2.473 1.00
317_N 320_K 2.441 1.00
355_I 359_L 2.421 1.00
314_R 431_S 2.417 1.00
312_C 416_R 2.391 1.00
299_L 333_V 2.381 1.00
157_I 194_V 2.356 1.00
20_K 469_D 2.295 1.00
359_L 414_I 2.283 1.00
371_G 396_K 2.197 0.99
510_I 514_V 2.166 0.99
312_C 420_T 2.138 0.99
370_L 403_V 2.137 0.99
405_A 409_V 2.084 0.99
21_T 89_L 2.065 0.99
71_S 75_S 2.064 0.99
499_V 509_V 2.008 0.99
481_D 484_L 1.971 0.99
168_I 182_F 1.969 0.99
220_G 223_G 1.959 0.99
456_L 525_M 1.949 0.98
456_L 521_M 1.919 0.98
228_G 364_F 1.893 0.98
399_M 402_V 1.86 0.98
311_H 420_T 1.816 0.97
310_Y 318_R 1.815 0.97
317_N 432_E 1.814 0.97
458_A 493_N 1.798 0.97
470_N 493_N 1.789 0.97
405_A 408_F 1.781 0.97
354_V 358_L 1.773 0.97
463_D 484_L 1.747 0.96
418_A 422_F 1.745 0.96
410_V 414_I 1.721 0.96
302_L 305_A 1.717 0.96
165_F 186_I 1.685 0.95
402_V 406_I 1.653 0.95
355_I 414_I 1.653 0.95
158_V 187_S 1.645 0.94
458_A 489_A 1.619 0.94
161_L 182_F 1.616 0.94
229_T 357_A 1.611 0.94
455_I 510_I 1.605 0.93
186_I 190_V 1.604 0.93
456_L 524_L 1.602 0.93
456_L 468_L 1.602 0.93
355_I 418_A 1.596 0.93
39_G 70_G 1.583 0.93
169_R 172_D 1.582 0.93
493_N 514_V 1.576 0.93
307_L 311_H 1.539 0.91
297_A 360_T 1.53 0.91
495_A 498_V 1.51 0.90
31_F 188_S 1.49 0.90
230_G 243_L 1.484 0.89
467_S 478_S 1.481 0.89
229_T 354_V 1.473 0.89
367_M 372_V 1.472 0.89
470_N 517_V 1.471 0.89
296_P 363_G 1.459 0.88
89_L 156_I 1.449 0.88
486_N 489_A 1.438 0.87
477_L 521_M 1.434 0.87
156_I 160_M 1.428 0.87
168_I 178_G 1.414 0.86
365_T 369_V 1.41 0.86
307_L 408_F 1.406 0.86
294_K 376_N 1.398 0.85
426_T 429_R 1.395 0.85
459_L 508_V 1.392 0.85
334_V 382_I 1.389 0.85
417_F 421_R 1.386 0.84
34_I 38_I 1.386 0.84
161_L 190_V 1.385 0.84
315_P 318_R 1.384 0.84
319_H 322_K 1.383 0.84
323_G 341_L 1.382 0.84
472_I 514_V 1.375 0.84
175_A 337_T 1.375 0.84
313_A 426_T 1.374 0.84
314_R 430_D 1.367 0.83
248_R 378_D 1.364 0.83
498_V 508_V 1.356 0.83
15_F 18_L 1.347 0.82
161_L 186_I 1.342 0.82
508_V 514_V 1.342 0.82
310_Y 322_K 1.341 0.82
367_M 403_V 1.338 0.81
468_L 521_M 1.333 0.81
225_M 229_T 1.333 0.81
244_V 267_L 1.328 0.81
514_V 517_V 1.324 0.80
374_I 400_V 1.321 0.80
154_A 194_V 1.317 0.80
196_L 200_L 1.313 0.80
302_L 356_H 1.312 0.80
221_D 365_T 1.31 0.80
518_K 522_A 1.306 0.79
479_V 482_M 1.306 0.79
231_E 360_T 1.296 0.78
231_E 376_N 1.293 0.78
508_V 517_V 1.287 0.78
479_V 485_V 1.284 0.78
77_L 148_I 1.284 0.78
489_A 492_D 1.283 0.78
305_A 341_L 1.279 0.77
222_F 226_L 1.274 0.77
514_V 521_M 1.255 0.75
296_P 367_M 1.253 0.75
229_T 233_L 1.253 0.75
412_Y 420_T 1.253 0.75
250_T 254_G 1.245 0.74
419_I 426_T 1.235 0.74
233_L 357_A 1.23 0.73
459_L 477_L 1.218 0.72
24_L 87_L 1.217 0.72
314_R 425_K 1.215 0.72
351_V 355_I 1.21 0.71
23_M 241_H 1.204 0.71
295_M 298_F 1.195 0.70
322_K 326_I 1.188 0.69
102_F 106_A 1.186 0.69
487_V 491_K 1.184 0.69
490_L 508_V 1.181 0.69
189_L 193_L 1.179 0.69
310_Y 325_L 1.177 0.68
517_V 521_M 1.174 0.68
485_V 506_L 1.167 0.67
370_L 399_M 1.155 0.66
311_H 413_V 1.151 0.66
299_L 384_F 1.15 0.66
324_L 328_G 1.149 0.66
196_L 401_P 1.149 0.66
417_F 422_F 1.145 0.65
269_I 273_Q 1.141 0.65
425_K 430_D 1.14 0.65
154_A 158_V 1.14 0.65
154_A 191_M 1.139 0.65
298_F 332_C 1.139 0.65
22_F 180_T 1.138 0.65
305_A 309_M 1.129 0.64
227_F 291_S 1.126 0.63
104_G 154_A 1.125 0.63
317_N 430_D 1.124 0.63
168_I 186_I 1.122 0.63
157_I 191_M 1.121 0.63
419_I 429_R 1.121 0.63
89_L 175_A 1.121 0.63
224_P 229_T 1.12 0.63
297_A 407_W 1.118 0.63
106_A 139_L 1.117 0.63
510_I 517_V 1.114 0.62
79_V 102_F 1.109 0.62
317_N 332_C 1.109 0.62
165_F 182_F 1.107 0.61
219_A 317_N 1.105 0.61
108_M 150_G 1.103 0.61
470_N 518_K 1.103 0.61
335_G 380_N 1.103 0.61
221_D 369_V 1.097 0.60
459_L 521_M 1.092 0.60
289_F 293_G 1.086 0.59
181_R 184_P 1.081 0.59
412_Y 416_R 1.08 0.59
190_V 194_V 1.08 0.59
304_G 417_F 1.075 0.58
219_A 226_L 1.075 0.58
27_A 346_L 1.073 0.58
146_T 150_G 1.069 0.57
364_F 400_V 1.068 0.57
107_V 322_K 1.064 0.57
306_A 325_L 1.063 0.57
189_L 509_V 1.063 0.57
249_F 267_L 1.06 0.57
36_L 74_F 1.057 0.56
182_F 186_I 1.057 0.56
223_G 508_V 1.054 0.56
157_I 190_V 1.054 0.56
508_V 521_M 1.051 0.56
111_A 195_G 1.05 0.55
482_M 503_Q 1.049 0.55
482_M 500_Q 1.04 0.54
317_N 433_V 1.039 0.54
376_N 379_G 1.036 0.54
225_M 365_T 1.036 0.54
166_H 178_G 1.035 0.54
302_L 408_F 1.03 0.53
470_N 508_V 1.03 0.53
414_I 418_A 1.027 0.53
171_P 174_L 1.026 0.53
231_E 364_F 1.025 0.53
329_L 333_V 1.025 0.53
333_V 379_G 1.024 0.53
289_F 298_F 1.022 0.52
309_M 312_C 1.022 0.52
68_K 75_S 1.02 0.52
493_N 510_I 1.018 0.52
26_V 456_L 1.017 0.52
32_C 335_G 1.017 0.52
23_M 181_R 1.016 0.52
470_N 510_I 1.014 0.52
478_S 505_N 1.012 0.51
466_V 478_S 1.011 0.51
16_Q 19_G 1.011 0.51
315_P 412_Y 1.01 0.51
233_L 360_T 1.007 0.51
31_F 189_L 1.006 0.51
266_A 290_L 1.006 0.51
313_A 318_R 1.005 0.51
421_R 484_L 1.002 0.50
265_G 269_I 1.001 0.50
470_N 521_M 1.001 0.50
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3qnqA40.75851000.744Contact Map0.444
1ibaA10.175599.90.868Contact Map0.212
3bp3A20.154799.90.869Contact Map0.697
3ipjA20.160499.90.878Contact Map0.663
3wdoA10.790619.60.984Contact Map0.276
3pcoA20.1302130.985Contact Map0.08
3zqsA20.150912.10.986Contact Map0.137
4huqS10.296210.30.986Contact Map0.289
1gxuA10.10759.80.986Contact Map0.268
2kncA10.09437.10.987Contact Map0.611
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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