May 4, 2021 - We are working on upgrading the webserver, some pages may not work.
OPENSEQ.org
PTFLB - Fructose-like PTS system EIIBC component
UniProt: P32154 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11863
Length: 483 (465)
Sequences: 677
Seq/Len: 1.46
PTFLB
Paralog alert: 0.49 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: HRSA PTFBC PTFC1 PTFC2 PTFLB
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
356_A 456_N 4.542 1.00
8_V 63_V 4.33 1.00
55_E 59_A 3.983 1.00
212_V 236_L 3.849 1.00
201_A 205_A 3.536 1.00
34_L 101_S 3.484 1.00
27_L 38_I 3.26 1.00
360_A 368_F 3.106 1.00
189_I 233_A 3.094 1.00
317_F 407_T 3.078 1.00
348_V 449_L 3.033 1.00
243_F 272_V 2.979 1.00
34_L 97_D 2.977 1.00
39_K 56_E 2.94 1.00
361_T 376_A 2.934 1.00
317_F 323_I 2.896 1.00
97_D 101_S 2.852 1.00
193_G 236_L 2.787 1.00
95_N 98_Q 2.777 1.00
9_A 40_V 2.716 1.00
91_Q 95_N 2.706 1.00
142_F 210_F 2.7 1.00
48_V 51_R 2.628 1.00
313_A 407_T 2.606 1.00
31_A 38_I 2.604 1.00
187_F 214_Y 2.558 1.00
131_H 199_S 2.426 1.00
416_L 420_F 2.406 1.00
33_S 97_D 2.399 1.00
362_F 380_I 2.354 0.99
153_A 178_E 2.271 0.99
423_K 440_S 2.225 0.99
36_H 100_F 2.194 0.99
329_F 332_F 2.175 0.99
28_E 32_R 2.157 0.99
313_A 317_F 2.037 0.98
54_S 58_A 2.031 0.98
398_A 401_P 2.007 0.98
412_I 455_L 1.947 0.97
62_Y 103_L 1.914 0.97
39_K 50_N 1.913 0.97
150_V 185_F 1.841 0.96
6_R 59_A 1.824 0.96
331_C 346_V 1.822 0.96
52_L 57_I 1.798 0.95
195_Y 198_S 1.753 0.94
444_S 447_L 1.753 0.94
138_A 206_F 1.722 0.94
30_K 33_S 1.693 0.93
186_T 214_Y 1.686 0.93
25_E 28_E 1.671 0.92
102_E 106_N 1.656 0.92
316_A 388_T 1.621 0.91
359_L 453_L 1.615 0.91
316_A 410_G 1.608 0.90
191_I 210_F 1.598 0.90
412_I 451_I 1.585 0.89
207_A 270_L 1.573 0.89
441_P 444_S 1.565 0.89
355_V 453_L 1.554 0.88
286_S 290_G 1.554 0.88
184_G 211_L 1.553 0.88
30_K 34_L 1.547 0.88
80_A 111_A 1.545 0.88
235_V 239_A 1.544 0.88
197_A 240_I 1.522 0.87
45_S 203_K 1.518 0.87
24_A 28_E 1.513 0.86
440_S 444_S 1.506 0.86
309_F 415_V 1.49 0.85
285_M 326_T 1.469 0.84
316_A 353_P 1.456 0.83
451_I 455_L 1.44 0.82
348_V 413_T 1.434 0.82
370_R 373_K 1.429 0.82
420_F 444_S 1.415 0.81
347_G 350_A 1.414 0.81
83_K 109_L 1.414 0.81
138_A 195_Y 1.399 0.80
288_L 291_G 1.399 0.80
347_G 386_V 1.393 0.79
17_I 258_P 1.386 0.79
319_M 388_T 1.378 0.78
209_A 240_I 1.361 0.77
162_P 165_D 1.358 0.77
350_A 386_V 1.357 0.77
358_G 362_F 1.349 0.76
403_I 407_T 1.344 0.76
92_A 95_N 1.34 0.76
422_I 444_S 1.322 0.74
29_Q 371_Q 1.32 0.74
231_L 235_V 1.319 0.74
328_W 335_L 1.318 0.74
144_I 375_A 1.316 0.74
315_L 386_V 1.314 0.74
34_L 100_F 1.313 0.73
178_E 182_Y 1.312 0.73
62_Y 104_P 1.31 0.73
244_V 269_T 1.309 0.73
30_K 97_D 1.298 0.72
357_A 388_T 1.294 0.72
7_I 100_F 1.294 0.72
208_P 270_L 1.294 0.72
7_I 31_A 1.286 0.71
374_E 377_S 1.284 0.71
244_V 265_I 1.273 0.70
328_W 343_Y 1.267 0.70
416_L 419_A 1.262 0.69
65_L 84_V 1.261 0.69
62_Y 85_Y 1.258 0.69
302_S 305_F 1.258 0.69
408_L 459_F 1.252 0.68
416_L 444_S 1.252 0.68
347_G 351_L 1.248 0.68
233_A 303_M 1.246 0.68
351_L 386_V 1.245 0.68
364_A 367_L 1.244 0.68
62_Y 96_I 1.242 0.67
245_F 249_K 1.241 0.67
358_G 380_I 1.238 0.67
92_A 98_Q 1.237 0.67
313_A 349_V 1.235 0.67
194_A 210_F 1.233 0.67
447_L 451_I 1.228 0.66
76_R 110_F 1.225 0.66
247_F 269_T 1.225 0.66
408_L 412_I 1.222 0.66
53_S 56_E 1.222 0.66
127_S 131_H 1.218 0.65
408_L 451_I 1.216 0.65
42_T 50_N 1.214 0.65
290_G 294_H 1.213 0.65
239_A 243_F 1.208 0.64
96_I 100_F 1.204 0.64
27_L 372_E 1.199 0.63
343_Y 346_V 1.197 0.63
87_I 91_Q 1.193 0.63
30_K 96_I 1.185 0.62
311_V 346_V 1.183 0.62
439_M 451_I 1.183 0.62
174_T 178_E 1.181 0.62
145_G 270_L 1.179 0.61
247_F 265_I 1.174 0.61
265_I 269_T 1.171 0.61
7_I 38_I 1.17 0.61
210_F 214_Y 1.166 0.60
200_I 242_Y 1.165 0.60
45_S 261_G 1.165 0.60
243_F 273_F 1.163 0.60
317_F 349_V 1.16 0.60
313_A 411_G 1.157 0.59
368_F 461_I 1.155 0.59
408_L 463_L 1.154 0.59
448_V 451_I 1.153 0.59
364_A 461_I 1.148 0.58
6_R 37_T 1.145 0.58
80_A 112_A 1.143 0.58
309_F 312_G 1.128 0.56
315_L 350_A 1.126 0.56
29_Q 32_R 1.117 0.55
89_I 92_A 1.112 0.55
315_L 351_L 1.111 0.55
319_M 354_P 1.103 0.54
26_A 93_L 1.103 0.54
10_I 65_L 1.101 0.54
388_T 410_G 1.096 0.53
291_G 295_F 1.095 0.53
315_L 347_G 1.094 0.53
268_V 272_V 1.092 0.53
292_L 295_F 1.09 0.53
352_M 413_T 1.089 0.52
192_M 196_I 1.089 0.52
303_M 306_A 1.086 0.52
7_I 36_H 1.082 0.52
32_R 443_G 1.081 0.52
352_M 410_G 1.076 0.51
57_I 82_K 1.075 0.51
252_L 260_L 1.069 0.50
287_D 291_G 1.066 0.50
140_L 260_L 1.06 0.49
417_V 422_I 1.06 0.49
453_L 457_I 1.058 0.49
428_G 433_D 1.056 0.49
45_S 262_S 1.054 0.49
187_F 342_W 1.052 0.49
424_R 445_F 1.047 0.48
85_Y 210_F 1.043 0.48
149_L 177_V 1.043 0.48
91_Q 102_E 1.041 0.48
422_I 440_S 1.037 0.47
134_A 198_S 1.035 0.47
427_P 430_G 1.034 0.47
28_E 56_E 1.033 0.47
12_N 69_R 1.026 0.46
6_R 61_D 1.024 0.46
269_T 273_F 1.023 0.46
328_W 332_F 1.018 0.45
408_L 455_L 1.016 0.45
98_Q 102_E 1.014 0.45
260_L 264_L 1.014 0.45
209_A 213_C 1.013 0.45
29_Q 33_S 1.009 0.44
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2r48A10.21741000.785Contact Map0.76
2r4qA10.21741000.828Contact Map0.746
3qnqA40.7641000.835Contact Map0.457
2kyrA10.22771000.838Contact Map0.671
2m1zA10.21951000.847Contact Map0.593
4mgeA20.225799.70.907Contact Map0.525
1e2bA10.215399.60.911Contact Map0.459
2l2qA10.211299.50.919Contact Map0.352
3czcA10.196798.90.939Contact Map0.513
1tvmA10.202998.60.944Contact Map0.219
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
Page generated in 0.0939 seconds.