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OPENSEQ.org
PTFBC - PTS system fructose-specific EIIBC component
UniProt: P20966 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10336
Length: 563 (545)
Sequences: 674
Seq/Len: 1.24
PTFBC
Paralog alert: 0.47 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: HRSA PTFBC PTFC1 PTFC2 PTFLB
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
443_A 544_A 4.601 1.00
107_V 162_V 4.341 1.00
305_G 322_A 3.808 1.00
294_A 298_G 3.759 1.00
154_E 158_A 3.69 1.00
161_L 199_A 3.678 1.00
133_R 197_D 3.483 1.00
435_A 537_I 3.314 1.00
447_A 455_F 3.152 1.00
282_V 319_G 3.029 1.00
448_T 463_G 2.946 1.00
404_T 494_I 2.911 1.00
133_R 193_A 2.895 1.00
191_K 194_Q 2.834 1.00
286_A 322_A 2.811 1.00
147_A 150_A 2.792 1.00
240_M 303_L 2.787 1.00
126_I 137_V 2.781 1.00
193_A 197_D 2.779 1.00
404_T 410_V 2.713 1.00
229_H 292_S 2.667 1.00
329_I 359_V 2.661 1.00
130_A 137_V 2.608 1.00
108_A 139_V 2.583 1.00
280_L 307_M 2.564 1.00
187_L 191_K 2.52 1.00
138_K 155_E 2.486 1.00
400_G 494_I 2.483 1.00
251_S 270_M 2.406 0.99
495_V 547_A 2.352 0.99
510_K 528_T 2.334 0.99
449_M 467_L 2.309 0.99
503_I 507_I 2.266 0.99
132_K 193_A 2.245 0.99
416_A 419_V 2.236 0.99
127_E 131_K 2.156 0.98
153_P 157_A 2.008 0.97
485_D 488_R 1.981 0.97
198_K 202_E 1.963 0.97
236_Y 299_L 1.892 0.96
248_I 278_F 1.882 0.95
400_G 404_T 1.855 0.95
135_W 196_L 1.845 0.95
105_R 158_A 1.827 0.94
197_D 201_A 1.821 0.94
451_A 549_A 1.81 0.94
532_G 535_V 1.784 0.93
288_Y 291_F 1.744 0.92
290_A 326_A 1.669 0.90
279_A 307_M 1.662 0.90
284_V 303_L 1.662 0.90
495_V 539_A 1.661 0.90
403_C 475_S 1.658 0.90
138_K 149_N 1.652 0.89
124_E 127_E 1.631 0.88
418_G 433_I 1.619 0.88
442_L 541_T 1.617 0.88
455_F 549_A 1.607 0.87
337_L 351_I 1.597 0.87
444_M 548_Y 1.589 0.87
129_E 132_K 1.581 0.86
300_T 357_S 1.58 0.86
403_C 497_G 1.559 0.85
129_E 133_R 1.558 0.85
499_L 543_V 1.547 0.85
444_M 475_S 1.541 0.84
144_S 296_R 1.518 0.83
434_M 473_F 1.516 0.83
373_A 377_E 1.514 0.83
123_A 127_E 1.491 0.82
277_A 304_I 1.487 0.81
372_V 413_A 1.48 0.81
437_G 473_F 1.473 0.80
396_A 502_A 1.466 0.80
403_C 440_P 1.465 0.80
188_A 191_K 1.458 0.80
434_M 437_G 1.455 0.79
151_I 156_V 1.445 0.79
507_I 532_G 1.435 0.78
446_L 541_T 1.432 0.78
176_A 207_E 1.424 0.77
447_A 549_A 1.416 0.77
302_G 326_A 1.415 0.77
495_V 499_L 1.414 0.77
435_A 500_T 1.414 0.77
434_M 438_M 1.411 0.76
242_V 462_G 1.409 0.76
267_A 271_Q 1.384 0.74
499_L 539_A 1.381 0.74
179_P 205_P 1.38 0.74
128_T 458_A 1.373 0.74
270_M 274_G 1.371 0.73
438_M 473_F 1.371 0.73
402_M 473_F 1.37 0.73
321_I 325_L 1.369 0.73
529_P 532_G 1.361 0.73
415_Y 422_L 1.36 0.73
301_P 357_S 1.359 0.72
330_A 352_I 1.352 0.72
225_G 229_H 1.34 0.71
330_A 356_S 1.338 0.71
490_L 494_I 1.326 0.70
325_L 329_I 1.326 0.70
445_G 449_M 1.313 0.69
461_E 464_K 1.304 0.68
415_Y 430_M 1.301 0.68
144_S 348_P 1.3 0.68
430_M 433_I 1.299 0.68
404_T 436_A 1.275 0.65
236_Y 288_Y 1.275 0.65
503_I 532_G 1.275 0.65
188_A 194_Q 1.271 0.65
375_I 378_G 1.269 0.65
400_G 436_A 1.266 0.65
453_R 554_P 1.266 0.65
116_V 345_A 1.261 0.64
451_A 454_K 1.256 0.64
389_A 392_V 1.255 0.64
293_I 328_Y 1.251 0.63
457_K 460_Q 1.249 0.63
319_G 390_N 1.248 0.63
536_A 539_A 1.247 0.63
377_E 381_H 1.247 0.63
172_L 206_Y 1.243 0.63
398_L 433_I 1.237 0.62
402_M 438_M 1.228 0.61
489_V 548_Y 1.228 0.61
164_V 180_M 1.226 0.61
402_M 437_G 1.223 0.61
133_R 196_L 1.22 0.60
402_M 434_M 1.219 0.60
243_A 357_S 1.218 0.60
126_I 459_Q 1.203 0.59
254_F 265_L 1.2 0.58
390_N 393_L 1.192 0.58
267_A 422_L 1.19 0.58
106_V 200_V 1.184 0.57
511_L 533_Y 1.177 0.56
287_G 303_L 1.177 0.56
406_M 475_S 1.173 0.56
329_I 360_V 1.169 0.55
454_K 554_P 1.168 0.55
445_G 467_L 1.162 0.55
339_L 347_K 1.161 0.55
535_V 539_A 1.157 0.54
131_K 210_G 1.15 0.54
509_A 532_G 1.15 0.54
247_C 269_L 1.149 0.54
160_D 178_K 1.149 0.54
185_T 188_A 1.149 0.54
267_A 270_M 1.147 0.53
502_A 505_M 1.142 0.53
129_E 193_A 1.142 0.53
183_T 187_L 1.14 0.53
333_I 352_I 1.139 0.53
378_G 382_W 1.138 0.52
539_A 543_V 1.138 0.52
302_G 306_G 1.137 0.52
257_E 260_K 1.137 0.52
303_L 307_M 1.127 0.51
187_L 198_K 1.124 0.51
144_S 349_I 1.121 0.51
232_T 291_F 1.12 0.51
254_F 258_A 1.11 0.50
105_R 136_W 1.106 0.49
130_A 138_K 1.104 0.49
161_L 181_Y 1.102 0.49
528_T 532_G 1.094 0.48
236_Y 296_R 1.092 0.48
128_T 131_K 1.09 0.48
152_T 155_E 1.09 0.48
352_I 356_S 1.088 0.48
280_L 429_P 1.088 0.48
198_K 201_A 1.086 0.47
109_V 142_R 1.086 0.47
495_V 543_V 1.085 0.47
337_L 352_I 1.085 0.47
333_I 356_S 1.081 0.47
65_G 77_F 1.081 0.47
396_A 399_G 1.078 0.47
509_A 528_T 1.075 0.46
238_L 347_K 1.072 0.46
391_A 508_G 1.069 0.46
400_G 498_A 1.069 0.46
337_L 355_I 1.069 0.46
258_A 266_A 1.065 0.45
113_P 296_R 1.062 0.45
161_L 192_T 1.061 0.45
125_A 189_L 1.06 0.45
106_V 130_A 1.06 0.45
144_S 398_L 1.056 0.44
129_E 192_T 1.052 0.44
127_E 155_E 1.051 0.44
492_C 547_A 1.049 0.44
111_A 168_I 1.048 0.44
256_I 259_F 1.046 0.43
541_T 545_G 1.045 0.43
503_I 506_A 1.043 0.43
106_V 137_V 1.041 0.43
192_T 196_L 1.038 0.43
472_C 519_F 1.037 0.43
255_G 319_G 1.031 0.42
265_L 269_L 1.029 0.42
431_A 434_M 1.028 0.42
344_E 347_K 1.027 0.42
374_G 378_G 1.024 0.41
141_T 149_N 1.023 0.41
144_S 301_P 1.023 0.41
485_D 552_K 1.02 0.41
181_Y 303_L 1.019 0.41
252_F 513_A 1.019 0.41
232_T 236_Y 1.018 0.41
108_A 137_V 1.017 0.41
308_L 365_I 1.014 0.40
415_Y 419_V 1.014 0.40
176_A 208_P 1.01 0.40
458_A 550_F 1.01 0.40
161_L 200_V 1.009 0.40
254_F 269_L 1.007 0.40
347_K 351_I 1.004 0.39
379_L 382_W 1.001 0.39
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2r48A10.18651000.813Contact Map0.745
2r4qA10.18651000.85Contact Map0.749
3qnqA40.62881000.854Contact Map0.454
2kyrA10.19541000.862Contact Map0.647
2m1zA10.18471000.869Contact Map0.605
4mgeA20.193699.70.919Contact Map0.545
1e2bA10.179499.60.925Contact Map0.496
2l2qA10.181299.50.93Contact Map0.364
3czcA10.193698.80.949Contact Map0.522
1tvmA10.198998.10.959Contact Map0.249
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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