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OPENSEQ.org
HYFF - Hydrogenase-4 component F
UniProt: P77437 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG14214
Length: 526 (488)
Sequences: 6189
Seq/Len: 12.68
HYFF
Paralog alert: 0.86 [within 20: 0.60] - ratio of genomes with paralogs
Cluster includes: HYCC HYFB HYFD HYFF NUOL NUOM NUON
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
128_L 141_A 4.348 1.00
73_D 271_R 4.292 1.00
17_F 42_G 4.065 1.00
346_N 401_N 3.537 1.00
167_K 244_D 3.366 1.00
353_L 390_G 3.34 1.00
44_T 88_F 3.008 1.00
309_D 368_R 2.975 1.00
83_L 130_V 2.761 1.00
15_L 124_F 2.72 1.00
87_G 123_L 2.706 1.00
80_L 131_T 2.68 1.00
343_H 405_S 2.563 1.00
388_G 440_A 2.502 1.00
308_R 371_N 2.487 1.00
301_A 320_E 2.467 1.00
94_S 115_Y 2.435 1.00
47_L 85_V 2.426 1.00
246_H 262_L 2.398 1.00
135_L 272_Y 2.383 1.00
367_T 372_V 2.365 1.00
154_G 165_A 2.325 1.00
47_L 84_G 2.172 1.00
384_A 445_M 2.169 1.00
235_L 326_A 2.153 1.00
182_G 224_F 2.152 1.00
313_L 370_L 2.119 1.00
246_H 254_S 2.086 1.00
329_L 340_A 2.048 1.00
369_D 372_V 2.032 1.00
349_L 471_M 2.022 1.00
432_V 435_G 2.01 1.00
132_S 137_V 1.997 1.00
80_L 127_L 1.952 1.00
270_I 329_L 1.949 1.00
50_A 84_G 1.947 1.00
370_L 443_V 1.946 1.00
391_A 436_L 1.943 1.00
293_G 330_G 1.942 1.00
226_L 289_L 1.918 1.00
202_F 205_E 1.901 1.00
305_L 434_A 1.871 1.00
377_L 384_A 1.85 1.00
383_T 456_D 1.825 1.00
236_F 295_L 1.816 1.00
297_V 428_L 1.809 1.00
392_L 397_M 1.791 1.00
470_V 474_A 1.777 1.00
384_A 440_A 1.761 1.00
339_A 412_A 1.738 1.00
47_L 51_L 1.736 1.00
287_R 291_I 1.717 1.00
76_G 267_L 1.708 1.00
331_I 413_G 1.705 1.00
182_G 228_G 1.701 1.00
125_T 144_A 1.691 1.00
51_L 55_Q 1.684 1.00
78_L 345_L 1.662 1.00
82_I 348_S 1.656 1.00
342_L 405_S 1.635 1.00
262_L 318_S 1.633 1.00
226_L 230_G 1.63 1.00
65_A 69_W 1.629 1.00
380_M 383_T 1.623 1.00
317_S 435_G 1.614 1.00
48_I 52_W 1.613 1.00
308_R 369_D 1.613 1.00
10_L 49_L 1.605 1.00
234_G 242_L 1.597 1.00
413_G 422_I 1.561 1.00
54_V 81_A 1.548 1.00
382_F 466_V 1.53 1.00
86_I 352_T 1.519 1.00
310_I 439_M 1.519 1.00
328_E 409_T 1.516 1.00
459_W 463_S 1.512 1.00
11_L 124_F 1.51 1.00
438_R 442_R 1.501 1.00
179_G 183_T 1.494 1.00
163_E 167_K 1.492 1.00
171_I 240_A 1.491 1.00
229_F 268_V 1.489 1.00
362_L 368_R 1.485 1.00
331_I 336_G 1.467 1.00
298_A 428_L 1.463 1.00
152_L 253_V 1.456 1.00
387_F 391_A 1.455 1.00
314_L 358_S 1.452 1.00
299_V 303_F 1.444 1.00
291_I 295_L 1.443 1.00
72_I 131_T 1.442 1.00
86_I 259_A 1.441 1.00
223_V 227_I 1.431 1.00
138_M 268_V 1.43 1.00
78_L 341_L 1.427 1.00
161_S 248_E 1.419 1.00
338_F 488_A 1.413 1.00
391_A 440_A 1.412 1.00
41_L 45_L 1.402 0.99
44_T 48_I 1.4 0.99
458_G 461_T 1.399 0.99
13_T 42_G 1.397 0.99
13_T 45_L 1.393 0.99
216_T 220_L 1.391 0.99
230_G 235_L 1.388 0.99
478_H 482_P 1.383 0.99
389_G 469_L 1.372 0.99
488_A 492_T 1.37 0.99
327_V 425_L 1.366 0.99
387_F 439_M 1.358 0.99
307_Q 312_R 1.352 0.99
176_V 179_G 1.35 0.99
164_A 248_E 1.344 0.99
84_G 127_L 1.339 0.99
387_F 444_L 1.337 0.99
66_A 69_W 1.333 0.99
91_G 119_F 1.328 0.99
270_I 340_A 1.327 0.99
73_D 76_G 1.318 0.99
475_M 483_V 1.315 0.99
86_I 348_S 1.313 0.99
478_H 481_Q 1.312 0.99
71_H 74_G 1.302 0.99
96_G 460_L 1.294 0.99
235_L 292_F 1.292 0.99
160_S 248_E 1.289 0.99
219_L 223_V 1.282 0.99
251_S 311_K 1.282 0.99
244_D 312_R 1.269 0.99
485_R 488_A 1.26 0.99
290_L 330_G 1.257 0.99
65_A 68_L 1.255 0.99
243_P 316_Y 1.254 0.99
135_L 206_V 1.253 0.99
118_F 151_F 1.252 0.99
242_L 246_H 1.25 0.99
467_I 471_M 1.243 0.98
390_G 472_M 1.228 0.98
45_L 49_L 1.225 0.98
72_I 77_G 1.221 0.98
139_W 180_L 1.221 0.98
187_Y 191_A 1.214 0.98
270_I 337_I 1.201 0.98
474_A 478_H 1.199 0.98
346_N 405_S 1.194 0.98
361_V 376_M 1.192 0.98
83_L 127_L 1.18 0.98
420_L 423_V 1.175 0.98
182_G 221_A 1.174 0.98
185_L 217_L 1.174 0.98
328_E 336_G 1.169 0.97
89_L 467_I 1.163 0.97
459_W 462_T 1.159 0.97
269_L 326_A 1.157 0.97
370_L 442_R 1.155 0.97
149_S 261_L 1.152 0.97
133_N 204_S 1.149 0.97
423_V 427_L 1.145 0.97
363_L 455_G 1.143 0.97
129_V 264_C 1.138 0.97
119_F 256_L 1.137 0.97
290_L 327_V 1.134 0.97
345_L 483_V 1.129 0.97
350_A 393_A 1.128 0.97
11_L 131_T 1.126 0.97
399_P 477_T 1.126 0.97
481_Q 485_R 1.12 0.96
231_T 238_M 1.119 0.96
33_R 36_T 1.117 0.96
318_S 347_H 1.116 0.96
75_L 270_I 1.115 0.96
71_H 133_N 1.115 0.96
243_P 307_Q 1.113 0.96
184_V 188_A 1.105 0.96
28_R 31_A 1.101 0.96
120_H 124_F 1.096 0.96
360_N 457_L 1.093 0.96
293_G 326_A 1.092 0.96
255_A 356_C 1.09 0.96
373_V 443_V 1.088 0.96
431_L 435_G 1.081 0.95
325_V 340_A 1.077 0.95
70_L 131_T 1.076 0.95
185_L 221_A 1.073 0.95
389_G 473_L 1.069 0.95
126_M 145_T 1.068 0.95
385_V 444_L 1.067 0.95
183_T 225_L 1.064 0.95
122_F 149_S 1.062 0.95
328_E 340_A 1.06 0.95
385_V 389_G 1.054 0.95
298_A 302_F 1.054 0.95
267_L 271_R 1.053 0.95
466_V 470_V 1.052 0.95
266_L 325_V 1.051 0.94
364_K 380_M 1.048 0.94
301_A 317_S 1.047 0.94
200_A 205_E 1.046 0.94
429_L 435_G 1.042 0.94
345_L 475_M 1.04 0.94
7_F 14_P 1.03 0.94
279_A 283_D 1.028 0.94
221_A 225_L 1.023 0.93
186_V 221_A 1.023 0.93
347_H 351_K 1.02 0.93
122_F 126_M 1.019 0.93
91_G 116_Y 1.018 0.93
476_G 480_P 1.017 0.93
258_S 432_V 1.016 0.93
135_L 183_T 1.015 0.93
328_E 339_A 1.014 0.93
489_G 492_T 1.014 0.93
317_S 347_H 1.012 0.93
8_A 12_L 1.01 0.93
243_P 319_V 1.01 0.93
440_A 445_M 1.009 0.93
464_P 467_I 1.005 0.93
426_L 429_L 1.005 0.93
150_A 172_C 1.004 0.93
471_M 475_M 1.004 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3rkoM20.90871000.279Contact Map0.739
4he8M20.87451000.3Contact Map0.689
4he8L20.93921000.306Contact Map0.753
3rkoN20.89351000.319Contact Map0.66
3rkoL20.9431000.321Contact Map0.739
4he8N20.80991000.399Contact Map0.686
4he8K20.174919.40.975Contact Map0.782
3rkoK20.182511.20.978Contact Map0.518
1ji6A10.11791.90.985Contact Map0.454
3j1zP20.38971.80.985Contact Map0.185
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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