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RFAF - ADP-heptose--LPS heptosyltransferase 2
UniProt: P37692 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12210
Length: 348 (341)
Sequences: 2543
Seq/Len: 7.46
RFAF
Paralog alert: 0.82 [within 20: 0.45] - ratio of genomes with paralogs
Cluster includes: RFAC RFAF RFAQ WAAU
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
34_D 81_Y 4.433 1.00
184_G 262_C 3.859 1.00
264_A 339_L 3.661 1.00
2_K 32_I 3.015 1.00
201_H 332_P 2.842 1.00
208_Q 336_L 2.832 1.00
185_F 206_A 2.749 1.00
314_V 319_A 2.742 1.00
311_Y 319_A 2.742 1.00
53_E 79_K 2.709 1.00
202_Y 268_N 2.622 1.00
311_Y 314_V 2.6 1.00
255_A 272_L 2.568 1.00
311_Y 326_S 2.553 1.00
184_G 259_I 2.549 1.00
25_Q 52_N 2.518 1.00
3_I 31_A 2.479 1.00
273_M 285_A 2.466 1.00
319_A 326_S 2.431 1.00
314_V 326_S 2.42 1.00
199_H 230_E 2.362 1.00
251_Q 254_Q 2.264 1.00
205_L 266_V 2.18 1.00
197_W 335_V 2.167 1.00
32_I 52_N 2.119 1.00
205_L 336_L 2.083 1.00
267_T 273_M 2.056 1.00
34_D 52_N 2.042 1.00
198_P 201_H 2.035 1.00
225_H 229_N 2.032 1.00
32_I 53_E 2.031 1.00
166_E 253_D 1.976 1.00
14_M 45_L 1.963 1.00
184_G 219_F 1.951 1.00
182_M 261_A 1.931 1.00
303_R 341_A 1.909 1.00
34_D 53_E 1.861 1.00
4_L 36_M 1.84 1.00
182_M 262_C 1.816 1.00
266_V 339_L 1.813 1.00
208_Q 333_Q 1.753 1.00
183_I 209_L 1.751 1.00
20_L 87_L 1.715 1.00
212_E 340_N 1.699 1.00
34_D 55_I 1.648 1.00
203_A 234_A 1.644 1.00
181_P 263_K 1.638 1.00
218_L 228_G 1.636 1.00
182_M 263_K 1.635 1.00
2_K 79_K 1.631 1.00
220_G 248_G 1.595 1.00
197_W 201_H 1.592 1.00
43_P 46_S 1.552 1.00
203_A 207_K 1.542 1.00
204_E 207_K 1.539 1.00
245_N 248_G 1.537 1.00
169_Y 254_Q 1.535 1.00
183_I 264_A 1.51 0.99
129_P 137_A 1.505 0.99
167_K 260_A 1.494 0.99
2_K 34_D 1.493 0.99
25_Q 33_I 1.47 0.99
256_V 275_V 1.469 0.99
5_V 21_Y 1.46 0.99
201_H 204_E 1.457 0.99
333_Q 337_E 1.456 0.99
171_C 261_A 1.447 0.99
17_S 87_L 1.442 0.99
228_G 247_A 1.44 0.99
208_Q 212_E 1.435 0.99
163_S 166_E 1.402 0.99
14_M 35_V 1.402 0.99
70_R 74_H 1.375 0.99
195_K 269_D 1.367 0.99
22_R 159_Q 1.343 0.98
182_M 217_V 1.343 0.98
207_K 236_N 1.341 0.98
197_W 202_Y 1.34 0.98
303_R 338_E 1.324 0.98
229_N 233_A 1.323 0.98
86_V 93_S 1.316 0.98
219_F 255_A 1.309 0.98
34_D 79_K 1.305 0.98
3_I 33_I 1.294 0.98
186_C 267_T 1.293 0.98
7_G 10_W 1.283 0.98
16_M 295_T 1.282 0.98
73_G 77_R 1.277 0.98
209_L 336_L 1.269 0.98
228_G 245_N 1.268 0.98
6_I 36_M 1.266 0.98
325_Q 329_D 1.265 0.97
264_A 284_V 1.264 0.97
42_R 46_S 1.263 0.97
183_I 214_Y 1.258 0.97
273_M 287_Y 1.257 0.97
166_E 254_Q 1.254 0.97
20_L 139_A 1.242 0.97
327_L 330_I 1.241 0.97
259_I 279_L 1.239 0.97
286_L 330_I 1.235 0.97
184_G 258_L 1.234 0.97
18_Q 48_M 1.233 0.97
25_Q 31_A 1.227 0.97
98_F 105_R 1.226 0.97
204_E 333_Q 1.211 0.96
37_A 54_A 1.21 0.96
265_I 276_A 1.21 0.96
220_G 245_N 1.206 0.96
205_L 208_Q 1.199 0.96
197_W 332_P 1.198 0.96
265_I 281_R 1.191 0.96
339_L 343_L 1.187 0.96
83_R 106_T 1.187 0.96
206_A 218_L 1.186 0.96
331_T 334_R 1.185 0.96
47_R 253_D 1.185 0.96
4_L 81_Y 1.178 0.96
222_A 225_H 1.172 0.96
196_R 268_N 1.171 0.96
39_A 58_P 1.166 0.95
180_R 215_Q 1.165 0.95
133_E 298_L 1.164 0.95
22_R 26_A 1.164 0.95
182_M 215_Q 1.162 0.95
35_V 45_L 1.161 0.95
186_C 219_F 1.16 0.95
199_H 231_I 1.154 0.95
299_S 302_A 1.148 0.95
267_T 285_A 1.142 0.95
186_C 270_S 1.139 0.95
137_A 140_Y 1.122 0.94
185_F 266_V 1.12 0.94
189_A 196_R 1.116 0.94
207_K 211_D 1.116 0.94
201_H 331_T 1.114 0.94
219_F 262_C 1.109 0.94
197_W 205_L 1.108 0.93
187_P 218_L 1.106 0.93
104_H 119_D 1.102 0.93
286_L 305_I 1.1 0.93
307_L 334_R 1.1 0.93
10_W 14_M 1.092 0.93
101_G 105_R 1.084 0.92
324_H 327_L 1.08 0.92
204_E 332_P 1.069 0.92
334_R 338_E 1.068 0.92
336_L 340_N 1.067 0.92
105_R 118_N 1.067 0.92
309_T 314_V 1.065 0.92
198_P 328_I 1.064 0.92
14_M 41_C 1.061 0.91
284_V 338_E 1.061 0.91
21_Y 35_V 1.06 0.91
266_V 284_V 1.06 0.91
22_R 50_E 1.058 0.91
229_N 245_N 1.058 0.91
338_E 341_A 1.057 0.91
11_V 41_C 1.052 0.91
306_R 312_H 1.051 0.91
164_E 168_S 1.048 0.91
305_I 330_I 1.035 0.90
313_K 319_A 1.034 0.90
217_V 258_L 1.032 0.90
262_C 281_R 1.032 0.90
188_G 268_N 1.028 0.90
86_V 89_N 1.025 0.89
205_L 332_P 1.023 0.89
84_A 100_A 1.02 0.89
269_D 287_Y 1.017 0.89
25_Q 30_Q 1.017 0.89
28_Y 31_A 1.016 0.89
284_V 339_L 1.015 0.89
189_A 268_N 1.012 0.89
102_I 105_R 1.011 0.89
186_C 295_T 1.003 0.88
200_Y 230_E 1.002 0.88
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1pswA10.94541000.213Contact Map0.745
3tovA20.95691000.259Contact Map0.754
2gt1A20.89371000.316Contact Map0.782
3ot5A40.933999.20.828Contact Map0.577
2yjnA10.908990.838Contact Map0.525
4fzrA10.790298.80.851Contact Map0.664
4hwgA10.890898.70.859Contact Map0.486
4amgA20.890898.60.86Contact Map0.536
1vgvA40.879398.60.861Contact Map0.717
3otgA10.870798.60.862Contact Map0.616
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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