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OPENSEQ.org
RFAC - Lipopolysaccharide heptosyltransferase 1
UniProt: P24173 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11189
Length: 319 (319)
Sequences: 2560
Seq/Len: 8.03
RFAC
Paralog alert: 0.85 [within 20: 0.45] - ratio of genomes with paralogs
Cluster includes: RFAC RFAF RFAQ WAAU
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
34_D 87_Y 4.626 1.00
182_V 254_A 4.135 1.00
256_F 316_L 3.752 1.00
297_C 303_N 3.547 1.00
205_L 313_L 3.042 1.00
2_R 32_K 2.984 1.00
53_R 85_E 2.815 1.00
183_F 203_I 2.81 1.00
198_H 309_S 2.794 1.00
199_W 260_V 2.691 1.00
25_Q 52_E 2.672 1.00
247_V 264_L 2.622 1.00
3_V 31_I 2.62 1.00
265_S 277_T 2.61 1.00
182_V 251_L 2.579 1.00
243_S 246_G 2.316 1.00
196_E 228_R 2.285 1.00
202_L 258_V 2.273 1.00
200_R 232_G 2.262 1.00
32_K 53_R 2.199 1.00
180_Y 253_G 2.179 1.00
195_P 198_H 2.161 1.00
259_S 265_S 2.135 1.00
32_K 52_E 2.131 1.00
223_E 227_K 2.119 1.00
202_L 313_L 2.103 1.00
34_D 52_E 2.094 1.00
14_V 45_P 2.046 1.00
194_W 312_V 2.035 1.00
258_V 316_L 2.012 1.00
34_D 53_R 1.989 1.00
181_A 206_L 1.952 1.00
4_L 36_V 1.922 1.00
182_V 216_P 1.914 1.00
20_A 93_A 1.872 1.00
180_Y 254_A 1.837 1.00
237_E 240_P 1.77 1.00
205_L 310_Q 1.759 1.00
209_S 317_S 1.732 1.00
138_Q 146_E 1.726 1.00
179_E 255_K 1.717 1.00
215_L 226_A 1.693 1.00
2_R 85_E 1.658 1.00
180_Y 255_K 1.64 1.00
194_W 198_H 1.597 1.00
34_D 55_I 1.592 1.00
2_R 34_D 1.581 1.00
43_Q 46_S 1.573 1.00
248_A 267_L 1.538 1.00
200_R 204_G 1.525 1.00
14_V 35_W 1.525 1.00
201_E 204_G 1.495 1.00
79_R 83_Q 1.494 1.00
181_A 256_F 1.453 0.99
204_G 233_F 1.439 0.99
257_V 273_R 1.43 0.99
310_Q 314_E 1.43 0.99
180_Y 214_K 1.425 0.99
111_K 129_N 1.424 0.99
6_V 36_V 1.423 0.99
205_L 209_S 1.416 0.99
17_T 93_A 1.411 0.99
226_A 239_L 1.403 0.99
25_Q 33_F 1.397 0.99
5_I 21_L 1.393 0.99
198_H 201_E 1.388 0.99
194_W 199_W 1.381 0.99
34_D 85_E 1.38 0.99
202_L 205_L 1.378 0.99
257_V 268_T 1.369 0.99
42_A 46_S 1.358 0.99
181_A 211_I 1.349 0.99
3_V 33_F 1.324 0.99
256_F 276_I 1.321 0.98
184_L 262_T 1.32 0.98
227_K 231_E 1.317 0.98
7_K 10_S 1.315 0.98
178_G 212_R 1.299 0.98
89_A 112_H 1.293 0.98
220_P 223_E 1.291 0.98
37_V 54_V 1.29 0.98
192_K 261_D 1.29 0.98
251_L 271_L 1.29 0.98
47_W 245_E 1.289 0.98
206_L 313_L 1.285 0.98
184_L 259_S 1.27 0.98
182_V 250_V 1.27 0.98
4_L 87_Y 1.268 0.98
165_I 246_G 1.267 0.98
291_G 294_Q 1.256 0.98
265_S 279_Y 1.253 0.98
76_K 80_E 1.247 0.98
216_P 247_V 1.246 0.98
302_E 306_N 1.245 0.98
185_H 215_L 1.235 0.97
18_L 48_H 1.235 0.97
201_E 310_Q 1.231 0.97
308_N 311_A 1.221 0.97
142_E 290_Y 1.21 0.97
198_H 308_N 1.207 0.97
184_L 216_P 1.207 0.97
193_H 260_V 1.206 0.97
226_A 237_E 1.205 0.97
16_H 287_I 1.193 0.97
167_Q 253_G 1.186 0.96
187_T 193_H 1.183 0.96
304_L 307_L 1.182 0.96
278_V 307_L 1.181 0.96
258_V 276_I 1.175 0.96
39_E 58_A 1.169 0.96
25_Q 31_I 1.169 0.96
311_A 315_K 1.169 0.96
146_E 150_K 1.162 0.96
92_D 99_S 1.158 0.96
35_W 45_P 1.151 0.96
259_S 277_T 1.149 0.96
214_K 250_V 1.146 0.95
194_W 309_S 1.146 0.95
90_V 107_A 1.144 0.95
203_I 215_L 1.135 0.95
194_W 202_L 1.131 0.95
196_E 229_L 1.124 0.95
22_T 26_Q 1.124 0.95
180_Y 212_R 1.123 0.95
11_M 41_F 1.116 0.95
183_F 258_V 1.109 0.94
204_G 208_D 1.103 0.94
301_R 304_L 1.102 0.94
10_S 14_V 1.102 0.94
261_D 279_Y 1.101 0.94
22_T 50_A 1.101 0.94
313_L 317_S 1.096 0.94
205_L 309_S 1.087 0.93
14_V 41_F 1.079 0.93
4_L 90_V 1.079 0.93
276_I 316_L 1.074 0.93
25_Q 30_G 1.07 0.93
21_L 35_W 1.067 0.93
315_K 318_S 1.064 0.93
216_P 254_A 1.063 0.92
254_A 273_R 1.063 0.92
276_I 315_K 1.061 0.92
193_H 225_R 1.056 0.92
307_L 311_A 1.054 0.92
296_V 303_N 1.054 0.92
10_S 188_T 1.049 0.92
28_I 31_I 1.049 0.92
186_A 260_V 1.048 0.92
105_R 111_K 1.043 0.92
201_E 309_S 1.043 0.92
310_Q 313_L 1.036 0.91
218_G 240_P 1.022 0.90
227_K 237_E 1.018 0.90
206_L 316_L 1.013 0.90
211_I 255_K 1.013 0.90
228_R 231_E 1.012 0.90
23_D 27_A 1.01 0.90
42_A 54_V 1.001 0.89
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2gt1A211000.193Contact Map0.775
3tovA20.95611000.268Contact Map0.799
1pswA10.95611000.27Contact Map0.773
3ot5A40.962499.10.819Contact Map0.593
2yjnA10.95398.80.835Contact Map0.536
4hwgA10.956198.60.849Contact Map0.471
4fzrA10.93198.50.85Contact Map0.674
3otgA10.921698.50.851Contact Map0.642
1v4vA20.927998.30.86Contact Map0.637
1vgvA40.937398.30.862Contact Map0.726
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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