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OPENSEQ.org
PFLD - Formate acetyltransferase 2
UniProt: P32674 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11910
Length: 765 (762)
Sequences: 769
Seq/Len: 1.01
PFLD
Paralog alert: 0.42 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: PFLB PFLD PFLF TDCE
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
281_Q 285_T 4.915 1.00
109_R 113_E 4.787 1.00
415_S 425_P 4.025 1.00
458_Y 589_I 3.856 1.00
87_L 108_K 3.641 1.00
631_A 637_F 3.462 1.00
724_E 728_R 3.131 1.00
288_E 293_L 3.051 1.00
211_E 234_R 2.98 1.00
269_L 300_L 2.956 1.00
230_A 237_A 2.946 1.00
228_T 231_E 2.926 1.00
248_C 297_L 2.922 1.00
466_R 600_E 2.844 1.00
372_T 376_M 2.684 1.00
244_F 293_L 2.588 0.99
230_A 253_Y 2.586 0.99
584_D 667_K 2.581 0.99
289_D 292_F 2.581 0.99
531_S 590_S 2.56 0.99
244_F 280_Y 2.517 0.99
353_Q 384_F 2.503 0.99
237_A 253_Y 2.486 0.99
277_L 281_Q 2.482 0.99
559_N 637_F 2.474 0.99
63_L 245_W 2.421 0.99
280_Y 293_L 2.353 0.99
284_L 288_E 2.326 0.99
77_M 86_L 2.326 0.99
248_C 296_L 2.325 0.99
134_D 181_Q 2.314 0.99
532_D 653_Q 2.313 0.99
49_Y 53_H 2.298 0.98
334_T 338_R 2.274 0.98
726_Q 755_D 2.26 0.98
470_S 600_E 2.256 0.98
582_D 663_K 2.252 0.98
751_K 755_D 2.246 0.98
239_H 242_Q 2.226 0.98
293_L 296_L 2.216 0.98
48_A 52_E 2.19 0.98
475_L 479_G 2.144 0.97
226_L 495_L 2.121 0.97
261_E 494_P 2.077 0.97
595_R 672_L 2.072 0.97
177_Q 181_Q 2.044 0.96
7_R 61_E 2.042 0.96
442_V 469_I 2.041 0.96
333_L 339_S 2.026 0.96
353_Q 381_T 2.026 0.96
280_Y 296_L 2.013 0.96
175_Q 179_H 2.012 0.96
173_V 217_C 1.998 0.96
575_R 578_K 1.977 0.95
350_D 377_K 1.974 0.95
178_Q 182_Q 1.962 0.95
272_F 348_C 1.928 0.94
282_T 286_Q 1.921 0.94
143_Q 628_V 1.895 0.94
719_A 723_R 1.873 0.93
530_L 593_L 1.871 0.93
174_A 178_Q 1.856 0.93
49_Y 52_E 1.837 0.92
606_N 610_G 1.826 0.92
228_T 232_I 1.826 0.92
175_Q 178_Q 1.821 0.92
724_E 731_D 1.809 0.91
726_Q 751_K 1.809 0.91
203_I 237_A 1.801 0.91
183_Q 186_N 1.793 0.91
299_S 302_V 1.786 0.91
301_W 348_C 1.784 0.91
58_I 67_N 1.783 0.91
22_E 55_E 1.782 0.91
80_E 111_Y 1.769 0.90
63_L 292_F 1.768 0.90
139_A 475_L 1.763 0.90
535_H 585_E 1.749 0.89
235_H 242_Q 1.745 0.89
447_L 557_K 1.745 0.89
174_A 177_Q 1.742 0.89
422_L 670_N 1.737 0.89
63_L 299_S 1.732 0.89
579_Y 679_L 1.712 0.88
466_R 596_H 1.706 0.88
557_K 560_F 1.701 0.88
547_L 568_V 1.699 0.88
169_L 226_L 1.697 0.87
461_L 534_L 1.689 0.87
353_Q 377_K 1.685 0.87
233_S 253_Y 1.675 0.87
662_L 712_I 1.665 0.86
225_E 494_P 1.661 0.86
505_E 611_Y 1.654 0.86
229_I 253_Y 1.652 0.85
55_E 232_I 1.651 0.85
170_G 239_H 1.641 0.85
379_A 698_A 1.64 0.85
538_K 543_E 1.633 0.85
179_H 182_Q 1.624 0.84
63_L 296_L 1.611 0.83
179_H 502_D 1.587 0.82
578_K 655_A 1.585 0.82
529_N 632_T 1.575 0.82
136_V 479_G 1.57 0.81
136_V 482_I 1.569 0.81
209_L 212_T 1.567 0.81
65_A 229_I 1.562 0.81
170_G 238_Q 1.561 0.81
368_A 706_Q 1.553 0.80
259_Q 307_I 1.551 0.80
566_E 569_R 1.549 0.80
180_C 217_C 1.547 0.80
106_E 109_R 1.542 0.80
243_T 288_E 1.521 0.78
288_E 296_L 1.521 0.78
539_G 544_Q 1.518 0.78
30_S 34_T 1.51 0.78
728_R 731_D 1.504 0.77
90_L 103_I 1.473 0.75
97_P 102_A 1.469 0.75
459_E 462_L 1.468 0.75
442_V 468_K 1.462 0.74
207_A 234_R 1.443 0.73
212_T 216_N 1.443 0.73
592_E 596_H 1.439 0.73
561_A 637_F 1.431 0.72
49_Y 80_E 1.43 0.72
376_M 695_R 1.426 0.72
227_L 238_Q 1.425 0.72
247_A 279_F 1.423 0.72
173_V 197_E 1.421 0.71
548_S 551_E 1.42 0.71
54_V 232_I 1.42 0.71
208_E 212_T 1.419 0.71
661_V 679_L 1.419 0.71
556_L 560_F 1.418 0.71
368_A 395_E 1.404 0.70
637_F 640_E 1.4 0.70
58_I 210_A 1.399 0.70
679_L 709_L 1.397 0.70
177_Q 180_C 1.392 0.69
37_E 45_K 1.38 0.68
443_M 537_L 1.373 0.68
210_A 229_I 1.371 0.67
663_K 707_L 1.369 0.67
531_S 586_V 1.368 0.67
229_I 237_A 1.362 0.67
480_S 522_V 1.353 0.66
235_H 239_H 1.352 0.66
297_L 348_C 1.345 0.65
443_M 461_L 1.343 0.65
423_S 500_I 1.341 0.65
502_D 516_R 1.34 0.65
600_E 603_K 1.335 0.64
171_E 174_A 1.327 0.64
134_D 138_A 1.323 0.63
272_F 328_A 1.317 0.63
241_P 250_L 1.314 0.63
382_I 391_I 1.314 0.63
103_I 108_K 1.31 0.62
272_F 276_M 1.306 0.62
273_D 345_S 1.303 0.62
326_Y 355_V 1.302 0.62
170_G 227_L 1.299 0.61
560_F 572_L 1.299 0.61
543_E 554_S 1.295 0.61
229_I 232_I 1.29 0.60
169_L 227_L 1.287 0.60
658_P 681_V 1.286 0.60
679_L 712_I 1.286 0.60
26_L 53_H 1.284 0.60
245_W 292_F 1.281 0.60
527_I 594_L 1.279 0.60
136_V 475_L 1.278 0.59
453_N 638_A 1.276 0.59
134_D 137_K 1.274 0.59
226_L 257_I 1.272 0.59
209_L 213_M 1.27 0.59
474_T 604_Y 1.267 0.58
198_A 609_G 1.264 0.58
231_E 235_H 1.26 0.58
297_L 344_L 1.255 0.57
173_V 177_Q 1.25 0.57
558_A 563_P 1.249 0.57
588_N 592_E 1.246 0.57
248_C 300_L 1.244 0.56
211_E 230_A 1.239 0.56
658_P 700_F 1.237 0.56
131_Q 200_Q 1.235 0.56
456_L 561_A 1.235 0.56
207_A 211_E 1.234 0.55
662_L 679_L 1.23 0.55
294_K 298_E 1.229 0.55
137_K 141_N 1.228 0.55
397_V 414_Y 1.224 0.54
727_Q 755_D 1.224 0.54
725_A 735_L 1.222 0.54
186_N 474_T 1.22 0.54
225_E 261_E 1.219 0.54
244_F 344_L 1.217 0.54
376_M 698_A 1.209 0.53
176_M 193_L 1.204 0.53
330_L 345_S 1.2 0.52
204_L 208_E 1.2 0.52
215_A 463_E 1.193 0.52
58_I 73_R 1.192 0.52
539_G 576_F 1.19 0.51
137_K 346_F 1.19 0.51
230_A 239_H 1.181 0.51
86_L 90_L 1.181 0.51
203_I 236_N 1.178 0.50
164_L 254_M 1.176 0.50
704_F 709_L 1.174 0.50
720_D 723_R 1.174 0.50
272_F 301_W 1.171 0.50
458_Y 538_K 1.167 0.49
208_E 234_R 1.157 0.48
225_E 228_T 1.154 0.48
119_W 274_Q 1.154 0.48
466_R 592_E 1.152 0.48
745_F 748_E 1.152 0.48
424_I 505_E 1.151 0.48
235_H 246_Q 1.15 0.48
497_S 504_L 1.149 0.48
86_L 89_E 1.149 0.48
106_E 113_E 1.146 0.47
197_E 201_K 1.143 0.47
181_Q 285_T 1.142 0.47
346_F 373_P 1.136 0.46
199_S 495_L 1.132 0.46
177_Q 197_E 1.129 0.46
529_N 634_D 1.125 0.45
77_M 310_L 1.124 0.45
189_Y 507_G 1.124 0.45
280_Y 344_L 1.123 0.45
341_V 374_F 1.121 0.45
142_T 454_A 1.119 0.45
381_T 384_F 1.118 0.45
457_T 461_L 1.115 0.44
232_I 235_H 1.114 0.44
91_D 108_K 1.112 0.44
173_V 200_Q 1.109 0.44
464_Q 468_K 1.105 0.43
10_T 14_A 1.104 0.43
750_S 753_I 1.102 0.43
556_L 611_Y 1.1 0.43
207_A 230_A 1.099 0.43
566_E 570_A 1.099 0.43
105_E 108_K 1.099 0.43
231_E 242_Q 1.097 0.43
34_T 42_R 1.094 0.42
457_T 542_F 1.094 0.42
281_Q 712_I 1.093 0.42
164_L 499_F 1.09 0.42
723_R 727_Q 1.089 0.42
570_A 573_I 1.086 0.42
25_L 110_I 1.084 0.42
223_R 227_L 1.083 0.41
35_E 118_Y 1.082 0.41
86_L 310_L 1.079 0.41
463_E 467_A 1.077 0.41
383_R 694_L 1.076 0.41
167_H 175_Q 1.07 0.40
277_L 342_N 1.068 0.40
232_I 246_Q 1.064 0.40
244_F 288_E 1.063 0.40
540_M 576_F 1.062 0.40
175_Q 182_Q 1.062 0.40
51_L 228_T 1.061 0.39
711_H 715_N 1.06 0.39
599_K 672_L 1.059 0.39
138_A 272_F 1.058 0.39
300_L 304_C 1.056 0.39
309_L 756_D 1.056 0.39
171_E 175_Q 1.056 0.39
464_Q 720_D 1.053 0.39
705_T 709_L 1.05 0.39
465_I 530_L 1.048 0.38
447_L 461_L 1.046 0.38
243_T 293_L 1.046 0.38
203_I 250_L 1.043 0.38
365_R 394_D 1.043 0.38
58_I 76_I 1.043 0.38
290_A 350_D 1.043 0.38
475_L 478_E 1.04 0.38
222_R 225_E 1.04 0.38
285_T 297_L 1.04 0.38
137_K 181_Q 1.037 0.37
678_L 711_H 1.037 0.37
3_N 6_S 1.034 0.37
502_D 505_E 1.031 0.37
447_L 756_D 1.031 0.37
62_E 65_A 1.03 0.37
286_Q 289_D 1.029 0.37
399_P 414_Y 1.027 0.37
34_T 45_K 1.027 0.37
56_I 209_L 1.026 0.36
596_H 600_E 1.026 0.36
507_G 756_D 1.023 0.36
294_K 347_L 1.023 0.36
727_Q 751_K 1.023 0.36
570_A 574_N 1.023 0.36
535_H 583_I 1.021 0.36
288_E 292_F 1.018 0.36
584_D 588_N 1.017 0.36
273_D 328_A 1.016 0.36
345_S 374_F 1.016 0.36
400_A 674_S 1.016 0.36
90_L 139_A 1.016 0.36
243_T 246_Q 1.014 0.35
527_I 531_S 1.014 0.35
443_M 534_L 1.013 0.35
73_R 76_I 1.013 0.35
373_P 377_K 1.012 0.35
63_L 248_C 1.012 0.35
310_L 522_V 1.012 0.35
269_L 272_F 1.011 0.35
754_Q 758_I 1.011 0.35
62_E 303_K 1.011 0.35
723_R 755_D 1.009 0.35
598_C 602_E 1.008 0.35
447_L 560_F 1.008 0.35
362_L 579_Y 1.008 0.35
213_M 502_D 1.006 0.35
715_N 729_P 1.005 0.35
237_A 250_L 1.005 0.35
586_V 684_T 1.005 0.35
65_A 253_Y 1.005 0.35
228_T 243_T 1.004 0.35
477_V 601_V 1 0.34
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2y8nA20.98951000.048Contact Map0.593
1r9dA20.99081000.055Contact Map0.6
2f3oA20.98171000.065Contact Map0.626
1h16A10.89281000.24Contact Map0.672
1h7bA10.68199.70.935Contact Map0.552
3ajfA40.11937.70.987Contact Map0.133
3ugsB20.130712.20.99Contact Map0.444
2kwvA10.052311.50.99Contact Map0.343
1wr1B10.073210.80.99Contact Map0.06
3ic3A40.107210.30.99Contact Map0.138
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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