May 4, 2021 - We are working on upgrading the webserver, some pages may not work.
OPENSEQ.org
PFLB - Formate acetyltransferase 1
UniProt: P09373 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10701
Length: 760 (748)
Sequences: 762
Seq/Len: 1.02
PFLB
Paralog alert: 0.50 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: PFLB PFLD PFLF TDCE
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
289_E 293_K 3.49 1.00
416_A 426_V 3.483 1.00
718_D 722_N 3.299 1.00
136_K 140_E 3.154 1.00
473_Y 577_L 3.107 1.00
535_D 640_R 2.846 1.00
572_P 654_K 2.792 1.00
297_I 302_A 2.661 1.00
490_Q 494_A 2.588 0.99
285_D 289_E 2.58 0.99
222_E 242_G 2.5 0.99
458_Q 461_P 2.496 0.99
298_T 301_E 2.489 0.99
238_A 245_I 2.478 0.99
238_A 261_F 2.428 0.99
31_Q 343_G 2.414 0.99
120_E 135_K 2.413 0.99
38_E 348_T 2.41 0.99
277_F 309_L 2.406 0.99
252_A 302_A 2.291 0.98
252_A 288_I 2.278 0.98
36_P 348_T 2.277 0.98
619_N 625_R 2.273 0.98
256_I 306_V 2.264 0.98
288_I 302_A 2.182 0.98
245_I 261_F 2.153 0.97
570_N 650_T 2.126 0.97
343_G 347_R 2.082 0.97
251_N 296_K 2.077 0.97
362_Y 394_D 2.071 0.97
81_T 85_H 2.065 0.97
458_Q 462_K 2.045 0.96
236_E 239_A 2.041 0.96
110_L 119_I 2.039 0.96
463_S 483_F 2.031 0.96
288_I 305_M 2.016 0.96
292_L 297_I 1.994 0.96
302_A 305_M 1.983 0.95
397_S 412_N 1.952 0.95
241_Y 261_F 1.934 0.94
485_D 588_K 1.931 0.94
24_V 192_Y 1.909 0.94
50_A 96_K 1.903 0.94
532_V 620_T 1.898 0.94
164_G 616_K 1.894 0.94
280_T 357_F 1.89 0.94
557_D 625_R 1.888 0.94
256_I 305_M 1.881 0.93
745_K 749_Q 1.878 0.93
359_N 387_F 1.875 0.93
17_K 21_Q 1.854 0.93
13_E 294_A 1.853 0.93
713_R 717_L 1.852 0.93
290_R 294_A 1.848 0.93
362_Y 391_V 1.83 0.92
41_E 387_F 1.826 0.92
90_I 221_R 1.82 0.92
720_M 745_K 1.812 0.92
269_S 509_A 1.807 0.91
80_S 84_S 1.805 0.91
389_A 687_A 1.795 0.91
567_F 666_Y 1.788 0.91
720_M 749_Q 1.782 0.91
18_G 21_Q 1.77 0.90
559_E 625_R 1.764 0.90
49_E 53_T 1.757 0.90
236_E 240_K 1.754 0.90
538_S 573_R 1.752 0.90
195_K 199_A 1.742 0.89
234_M 510_S 1.74 0.89
481_D 588_K 1.726 0.89
382_L 386_K 1.722 0.89
243_Y 250_T 1.716 0.88
41_E 383_N 1.714 0.88
191_D 246_S 1.712 0.88
160_C 490_Q 1.709 0.88
533_A 581_L 1.709 0.88
53_T 94_L 1.683 0.87
31_Q 34_Y 1.683 0.87
247_G 250_T 1.671 0.87
162_K 457_M 1.661 0.86
157_I 494_A 1.66 0.86
460_G 483_F 1.65 0.86
546_K 559_E 1.649 0.86
90_I 100_L 1.633 0.85
16_T 32_K 1.632 0.85
495_L 525_C 1.632 0.85
423_P 657_F 1.623 0.84
38_E 349_L 1.619 0.84
155_P 202_T 1.606 0.83
718_D 725_K 1.603 0.83
81_T 84_S 1.592 0.83
196_D 200_Q 1.576 0.82
19_D 25_N 1.575 0.82
191_D 247_G 1.555 0.81
249_A 258_W 1.549 0.80
342_M 348_T 1.547 0.80
594_T 598_A 1.547 0.80
563_E 566_Q 1.54 0.80
442_T 484_M 1.526 0.79
34_Y 343_G 1.523 0.79
350_V 384_F 1.514 0.78
96_K 308_H 1.511 0.78
303_Q 307_D 1.504 0.78
50_A 308_H 1.504 0.78
447_I 555_A 1.503 0.77
194_M 228_H 1.502 0.77
451_V 458_Q 1.489 0.77
469_D 546_K 1.487 0.76
74_F 78_V 1.486 0.76
466_I 475_E 1.486 0.76
200_Q 517_R 1.485 0.76
649_L 706_L 1.485 0.76
61_G 72_V 1.484 0.76
303_Q 356_R 1.482 0.76
542_Y 551_E 1.476 0.76
468_G 475_E 1.47 0.75
198_L 202_T 1.47 0.75
534_A 578_A 1.466 0.75
17_K 32_K 1.461 0.75
27_R 31_Q 1.458 0.74
666_Y 706_L 1.452 0.74
648_S 666_Y 1.45 0.74
267_V 316_V 1.423 0.72
354_S 384_F 1.415 0.71
280_T 337_E 1.411 0.71
460_G 463_S 1.406 0.71
474_D 477_M 1.399 0.70
473_Y 541_K 1.395 0.70
87_A 240_K 1.395 0.70
223_E 227_Q 1.387 0.69
470_V 546_K 1.387 0.69
157_I 497_I 1.386 0.69
233_Q 509_A 1.385 0.69
693_Y 703_G 1.381 0.69
280_T 284_L 1.38 0.68
588_K 591_K 1.376 0.68
481_D 584_R 1.376 0.68
392_S 400_Y 1.374 0.68
251_N 297_I 1.368 0.67
666_Y 703_G 1.366 0.67
199_A 203_S 1.364 0.67
362_Y 387_F 1.356 0.66
328_F 502_H 1.355 0.66
665_S 705_H 1.349 0.66
237_M 261_F 1.345 0.66
456_K 550_D 1.339 0.65
58_V 62_V 1.327 0.64
18_G 32_K 1.324 0.64
243_Y 247_G 1.321 0.64
744_T 747_Q 1.31 0.63
443_M 540_I 1.3 0.62
532_V 622_D 1.299 0.62
235_K 246_S 1.297 0.61
68_T 72_V 1.295 0.61
113_F 138_F 1.293 0.61
237_M 245_I 1.29 0.61
28_D 32_K 1.286 0.60
583_E 659_Y 1.283 0.60
255_A 287_Y 1.281 0.60
490_Q 493_T 1.278 0.60
98_V 237_M 1.274 0.59
256_I 309_L 1.271 0.59
252_A 353_N 1.27 0.59
224_I 517_R 1.27 0.59
443_M 476_V 1.265 0.59
190_I 234_M 1.264 0.58
636_P 736_Y 1.264 0.58
736_Y 743_L 1.262 0.58
386_K 684_T 1.252 0.57
649_L 666_Y 1.252 0.57
335_A 364_M 1.248 0.57
240_K 243_Y 1.247 0.57
722_N 725_K 1.247 0.57
719_A 729_L 1.246 0.57
281_S 354_S 1.245 0.57
96_K 305_M 1.243 0.57
424_M 515_H 1.242 0.56
77_A 131_D 1.24 0.56
238_A 247_G 1.238 0.56
45_A 300_Q 1.237 0.56
637_M 640_R 1.232 0.56
297_I 305_M 1.23 0.55
151_D 229_R 1.222 0.55
200_Q 203_S 1.22 0.54
291_D 296_K 1.218 0.54
479_R 483_F 1.217 0.54
336_T 399_Q 1.213 0.54
24_V 680_E 1.207 0.53
231_L 235_K 1.205 0.53
195_K 198_L 1.204 0.53
650_T 696_H 1.199 0.52
159_R 457_M 1.193 0.52
476_V 537_L 1.191 0.52
705_H 709_N 1.189 0.52
98_V 261_F 1.189 0.52
574_V 640_R 1.189 0.52
41_E 390_K 1.187 0.51
714_E 717_L 1.186 0.51
308_H 311_M 1.185 0.51
64_L 68_T 1.185 0.51
449_G 462_K 1.185 0.51
41_E 683_K 1.184 0.51
306_V 353_N 1.181 0.51
645_A 668_F 1.178 0.51
470_V 547_P 1.177 0.50
288_I 353_N 1.172 0.50
463_S 479_R 1.169 0.50
11_A 14_G 1.167 0.49
24_V 67_R 1.165 0.49
21_Q 32_K 1.162 0.49
660_A 750_D 1.158 0.49
406_M 415_Y 1.158 0.49
532_V 631_G 1.147 0.48
291_D 297_I 1.147 0.48
34_Y 282_T 1.144 0.47
27_R 343_G 1.139 0.47
374_I 384_F 1.137 0.47
95_E 312_K 1.137 0.47
336_T 373_T 1.134 0.46
157_I 490_Q 1.134 0.46
554_L 558_F 1.128 0.46
442_T 483_F 1.128 0.46
447_I 476_V 1.127 0.46
19_D 23_E 1.127 0.46
95_E 98_V 1.121 0.45
350_V 380_L 1.12 0.45
261_F 265_A 1.118 0.45
222_E 238_A 1.117 0.45
391_V 394_D 1.117 0.45
49_E 52_T 1.116 0.45
124_K 135_K 1.116 0.45
50_A 69_H 1.115 0.45
476_V 540_I 1.114 0.45
571_D 574_V 1.114 0.45
541_K 550_D 1.114 0.45
610_N 750_D 1.113 0.44
512_M 518_D 1.111 0.44
88_G 220_L 1.11 0.44
375_L 403_D 1.108 0.44
636_P 663_G 1.104 0.44
645_A 649_L 1.104 0.44
218_I 501_M 1.104 0.44
396_S 588_K 1.102 0.43
16_T 24_V 1.101 0.43
318_F 750_D 1.101 0.43
185_V 262_G 1.099 0.43
716_L 729_L 1.097 0.43
243_Y 254_E 1.097 0.43
62_V 90_I 1.097 0.43
11_A 250_T 1.096 0.43
19_D 32_K 1.095 0.43
408_P 415_Y 1.094 0.43
31_Q 36_P 1.091 0.42
92_K 760_M 1.091 0.42
197_K 519_V 1.09 0.42
281_S 337_E 1.087 0.42
534_A 574_V 1.085 0.42
50_A 62_V 1.083 0.42
481_D 580_D 1.081 0.42
466_I 555_A 1.08 0.41
318_F 495_L 1.08 0.41
748_Q 752_I 1.079 0.41
11_A 113_F 1.078 0.41
253_Q 301_E 1.075 0.41
277_F 280_T 1.075 0.41
466_I 470_V 1.075 0.41
24_V 97_I 1.072 0.41
96_K 253_Q 1.071 0.41
225_A 398_L 1.07 0.41
69_H 78_V 1.07 0.41
17_K 22_N 1.067 0.40
64_L 72_V 1.067 0.40
542_Y 573_R 1.066 0.40
106_L 317_R 1.065 0.40
233_Q 236_E 1.064 0.40
355_F 383_N 1.063 0.40
126_Y 526_G 1.062 0.40
124_K 129_E 1.062 0.40
548_I 552_D 1.062 0.40
449_G 464_E 1.06 0.40
90_I 106_L 1.06 0.40
299_E 359_N 1.059 0.40
62_V 72_V 1.059 0.40
350_V 377_S 1.059 0.40
23_E 32_K 1.058 0.39
739_R 742_S 1.057 0.39
50_A 94_L 1.056 0.39
190_I 235_K 1.054 0.39
360_T 364_M 1.054 0.39
237_M 240_K 1.053 0.39
110_L 319_L 1.052 0.39
64_L 67_R 1.05 0.39
35_T 42_S 1.049 0.39
334_W 371_N 1.048 0.39
190_I 262_G 1.048 0.39
310_V 357_F 1.047 0.39
119_I 123_C 1.047 0.39
641_D 755_T 1.046 0.38
285_D 351_T 1.045 0.38
273_A 318_F 1.043 0.38
447_I 466_I 1.041 0.38
716_L 723_P 1.04 0.38
50_A 311_M 1.04 0.38
413_D 656_P 1.04 0.38
733_V 751_V 1.04 0.38
574_V 580_D 1.039 0.38
17_K 23_E 1.039 0.38
13_E 38_E 1.038 0.38
542_Y 564_Y 1.037 0.38
58_V 315_M 1.035 0.37
50_A 59_M 1.034 0.37
467_K 475_E 1.034 0.37
79_A 119_I 1.033 0.37
10_T 13_E 1.031 0.37
252_A 297_I 1.029 0.37
73_D 104_A 1.029 0.37
306_V 357_F 1.028 0.37
221_R 237_M 1.028 0.37
440_A 532_V 1.027 0.37
230_A 233_Q 1.026 0.37
632_P 734_S 1.025 0.37
113_F 281_S 1.024 0.36
56_D 60_E 1.023 0.36
643_K 650_T 1.023 0.36
512_M 519_V 1.021 0.36
403_D 415_Y 1.02 0.36
657_F 664_I 1.02 0.36
446_A 476_V 1.017 0.36
463_S 470_V 1.017 0.36
524_A 560_I 1.015 0.36
441_K 452_D 1.014 0.36
636_P 648_S 1.013 0.36
696_H 703_G 1.012 0.35
32_K 38_E 1.012 0.35
547_P 554_L 1.012 0.35
58_V 72_V 1.011 0.35
194_M 218_I 1.01 0.35
705_H 755_T 1.008 0.35
447_I 750_D 1.007 0.35
375_L 415_Y 1.006 0.35
289_E 706_L 1.005 0.35
74_F 319_L 1.004 0.35
303_Q 363_T 1.004 0.35
454_K 626_A 1.003 0.35
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1h16A10.99741000.086Contact Map0.633
1r9dA20.92111000.181Contact Map0.601
2f3oA20.91181000.184Contact Map0.613
2y8nA20.95261000.187Contact Map0.6
1h7bA10.632999.60.941Contact Map0.514
1irqA20.081631.80.988Contact Map1
3ajfA40.117118.50.99Contact Map0.105
2o8rA20.090812.90.99Contact Map0.436
1xlyA20.119712.10.99Contact Map0.162
2hx6A10.081610.60.991Contact Map0.294
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
Page generated in 0.2101 seconds.