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OPENSEQ.org
HYFA - Hydrogenase-4 component A
UniProt: P23481 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11150
Length: 205 (188)
Sequences: 534
Seq/Len: 2.84
HYFA
Paralog alert: 0.73 [within 20: 0.36] - ratio of genomes with paralogs
Cluster includes: DMSB HYCB HYDN HYFA NRFC YDHX YDHY YGFS YNFG YSAA
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
152_L 160_R 3.043 1.00
21_A 161_A 2.364 1.00
12_C 18_C 2.312 1.00
12_C 15_C 2.263 1.00
68_I 97_I 2.126 1.00
15_C 18_C 2.106 1.00
62_V 87_L 2.037 1.00
69_S 78_N 2.03 1.00
12_C 162_C 1.918 0.99
34_H 38_A 1.9 0.99
152_L 157_A 1.864 0.99
156_P 159_V 1.856 0.99
18_C 162_C 1.778 0.99
15_C 162_C 1.736 0.98
177_R 180_K 1.696 0.98
171_T 174_S 1.668 0.98
25_V 151_F 1.667 0.98
174_S 177_R 1.663 0.98
14_G 18_C 1.647 0.98
7_A 50_V 1.615 0.97
13_T 164_N 1.604 0.97
23_S 28_T 1.56 0.96
33_Q 36_R 1.549 0.96
138_G 144_V 1.521 0.96
100_S 144_V 1.454 0.94
175_L 180_K 1.45 0.94
27_K 144_V 1.431 0.93
70_Q 75_I 1.401 0.92
14_G 162_C 1.362 0.91
49_V 52_H 1.362 0.91
74_A 153_P 1.337 0.90
36_R 39_L 1.335 0.90
13_T 163_P 1.325 0.89
124_E 128_T 1.322 0.89
7_A 167_L 1.278 0.87
125_N 128_T 1.277 0.87
86_K 101_G 1.268 0.86
21_A 83_I 1.221 0.83
12_C 163_P 1.218 0.83
124_E 127_P 1.217 0.83
106_N 118_S 1.204 0.82
17_T 164_N 1.2 0.82
81_L 138_G 1.186 0.81
87_L 90_V 1.185 0.81
73_D 186_A 1.182 0.81
110_Q 113_F 1.179 0.80
79_E 142_V 1.175 0.80
34_H 37_L 1.174 0.80
86_K 99_A 1.167 0.79
73_D 170_I 1.166 0.79
117_G 120_K 1.162 0.79
60_L 143_A 1.16 0.79
85_C 143_A 1.158 0.79
180_K 183_Q 1.157 0.79
175_L 182_K 1.152 0.78
71_R 76_Q 1.151 0.78
184_R 188_S 1.151 0.78
100_S 138_G 1.14 0.77
114_Q 117_G 1.137 0.77
78_N 89_A 1.127 0.76
24_D 161_A 1.125 0.76
13_T 16_N 1.123 0.76
14_G 17_T 1.117 0.75
176_Q 181_E 1.11 0.74
122_G 125_N 1.1 0.74
165_Q 168_R 1.096 0.73
180_K 184_R 1.096 0.73
174_S 179_M 1.094 0.73
53_H 168_R 1.093 0.73
79_E 97_I 1.09 0.73
36_R 40_A 1.084 0.72
63_C 82_C 1.072 0.71
62_V 65_V 1.065 0.70
34_H 40_A 1.064 0.70
68_I 77_L 1.058 0.69
83_I 86_K 1.042 0.68
21_A 157_A 1.041 0.68
175_L 179_M 1.039 0.67
53_H 76_Q 1.037 0.67
77_L 143_A 1.035 0.67
112_V 115_A 1.028 0.66
148_L 169_L 1.027 0.66
126_A 129_Q 1.027 0.66
85_C 91_V 1.019 0.65
94_F 184_R 1.013 0.64
159_V 167_L 1.01 0.64
65_V 68_I 1.009 0.64
25_V 53_H 1.001 0.63
20_A 69_S 1 0.63
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1ti6B60.80491000.508Contact Map0.362
1h0hB20.81461000.516Contact Map0.413
2ivfB10.81951000.524Contact Map0.443
1kqfB10.81951000.542Contact Map0.404
2vpzB20.81461000.561Contact Map0.449
1q16B10.819599.90.637Contact Map0.382
3mm5B20.819599.70.746Contact Map0.187
3gyxB60.736699.20.813Contact Map0.253
1rgvA10.380599.20.815Contact Map0.253
2fgoA10.390299.20.816Contact Map0.282
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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