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OPENSEQ.org
WCAF - Putative colanic acid biosynthesis acetyltransferase WcaF
UniProt: P0ACD2 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13574
Length: 182 (165)
Sequences: 1785
Seq/Len: 10.82
WCAF
Paralog alert: 0.53 [within 20: 0.02] - ratio of genomes with paralogs
Cluster includes: CAIE MAA PAAY THGA WBBJ WCAF YRDA
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
86_L 92_I 4.799 1.00
106_L 125_I 4.454 1.00
161_L 167_C 3.21 1.00
148_G 164_N 3.072 1.00
128_G 147_D 3.063 1.00
77_Y 97_H 3.029 1.00
152_G 168_R 2.925 1.00
53_K 73_T 2.481 1.00
83_D 103_K 2.422 1.00
107_C 135_T 2.403 1.00
96_A 129_E 2.376 1.00
140_A 153_A 2.357 1.00
147_D 163_A 2.312 1.00
55_G 76_D 2.297 1.00
133_L 139_V 2.26 1.00
73_T 93_T 2.242 1.00
106_L 127_I 2.163 1.00
129_E 147_D 2.126 1.00
168_R 175_I 1.992 1.00
130_K 148_G 1.965 1.00
76_D 96_A 1.901 1.00
149_T 163_A 1.889 1.00
113_H 150_V 1.879 1.00
97_H 130_K 1.835 1.00
126_V 144_T 1.722 1.00
93_T 126_V 1.69 1.00
98_S 129_E 1.676 1.00
151_V 161_L 1.667 1.00
149_T 165_V 1.663 1.00
155_S 172_A 1.601 1.00
57_N 77_Y 1.584 1.00
23_L 70_W 1.562 1.00
133_L 137_V 1.558 1.00
74_L 78_A 1.553 1.00
90_G 122_A 1.538 1.00
89_L 122_A 1.496 1.00
104_S 137_V 1.493 1.00
39_L 42_W 1.456 1.00
167_C 172_A 1.452 1.00
151_V 167_C 1.399 0.99
95_G 129_E 1.392 0.99
149_T 161_L 1.349 0.99
162_P 167_C 1.346 0.99
162_P 174_V 1.336 0.99
105_Y 136_D 1.334 0.99
128_G 144_T 1.316 0.99
52_A 73_T 1.314 0.99
113_H 166_V 1.297 0.99
100_I 106_L 1.274 0.99
78_A 129_E 1.272 0.99
94_I 100_I 1.269 0.99
165_V 174_V 1.261 0.99
128_G 149_T 1.255 0.99
98_S 147_D 1.245 0.98
103_K 136_D 1.244 0.98
54_I 58_V 1.227 0.98
106_L 139_V 1.22 0.98
167_C 174_V 1.201 0.98
32_F 36_P 1.182 0.98
27_V 31_I 1.165 0.97
75_G 96_A 1.159 0.97
85_N 103_K 1.159 0.97
42_W 45_F 1.158 0.97
27_V 50_F 1.158 0.97
29_A 38_V 1.156 0.97
78_A 98_S 1.148 0.97
78_A 96_A 1.144 0.97
66_I 72_L 1.141 0.97
73_T 91_E 1.114 0.96
64_V 72_L 1.104 0.96
166_V 178_R 1.096 0.96
80_V 100_I 1.085 0.96
165_V 177_E 1.075 0.95
140_A 158_F 1.071 0.95
98_S 131_C 1.036 0.94
105_Y 135_T 1.033 0.94
54_I 76_D 1.027 0.94
26_A 30_T 1.027 0.94
110_S 123_T 1.027 0.94
151_V 157_V 1.026 0.94
94_I 127_I 1.021 0.93
48_R 54_I 1.015 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3srtA20.9891000.318Contact Map0.838
3fttA111000.326Contact Map0.763
1krrA311000.327Contact Map0.865
2p2oA60.98351000.34Contact Map0.876
3hjjA30.9891000.342Contact Map0.87
3nz2A1211000.342Contact Map0.937
1ocxA30.97251000.363Contact Map0.865
3vbiA30.89561000.518Contact Map0.797
4hurA30.840799.90.536Contact Map0.77
2wlgA30.972599.90.537Contact Map0.814
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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