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OPENSEQ.org
RF3 - Peptide chain release factor 3
UniProt: P0A7I4 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12114
Length: 529 (511)
Sequences: 4331
Seq/Len: 8.48
RF3
Paralog alert: 0.85 [within 20: 0.03] - ratio of genomes with paralogs
Cluster includes: EFG LEPA RF3 TYPA
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
359_A 365_L 6.838 1.00
140_F 257_G 3.947 1.00
116_A 146_R 3.83 1.00
140_F 259_A 3.601 1.00
255_F 271_G 3.266 1.00
300_V 316_A 3.172 1.00
114_D 117_K 3.16 1.00
415_G 453_L 3.011 1.00
113_I 123_T 2.892 1.00
326_E 358_E 2.877 1.00
454_K 460_E 2.826 1.00
324_K 358_E 2.81 1.00
314_R 421_E 2.747 1.00
296_F 331_L 2.662 1.00
404_K 460_E 2.573 1.00
57_S 75_M 2.565 1.00
169_I 255_F 2.556 1.00
320_V 359_A 2.412 1.00
320_V 365_L 2.405 1.00
404_K 462_V 2.322 1.00
438_V 449_V 2.247 1.00
156_D 160_N 2.229 1.00
154_L 157_E 2.18 1.00
19_I 111_M 2.13 1.00
398_F 439_G 2.122 1.00
318_M 367_L 2.089 1.00
394_A 426_Q 2.083 1.00
370_H 373_I 2.075 1.00
29_I 112_V 2.063 1.00
30_T 86_L 2.051 1.00
114_D 154_L 2.002 1.00
331_L 342_I 1.991 1.00
46_V 55_A 1.986 1.00
451_A 455_S 1.93 1.00
61_E 65_Q 1.918 1.00
153_E 157_E 1.905 1.00
15_T 85_N 1.904 1.00
233_L 241_F 1.87 1.00
425_V 438_V 1.857 1.00
17_A 106_V 1.823 1.00
402_R 462_V 1.807 1.00
234_V 237_A 1.786 1.00
61_E 64_K 1.756 1.00
30_T 57_S 1.745 1.00
398_F 437_I 1.744 1.00
344_D 355_H 1.743 1.00
191_Y 267_H 1.741 1.00
298_G 318_M 1.736 1.00
115_A 144_L 1.732 1.00
72_T 87_L 1.729 1.00
16_F 84_V 1.72 1.00
108_C 276_A 1.712 1.00
115_A 141_M 1.711 1.00
173_I 190_T 1.703 1.00
167_A 251_I 1.671 1.00
59_W 71_T 1.67 1.00
347_T 356_V 1.669 1.00
126_L 129_V 1.665 1.00
226_Q 237_A 1.662 1.00
334_V 380_T 1.657 1.00
296_F 320_V 1.656 1.00
327_K 357_E 1.655 1.00
345_A 367_L 1.653 1.00
57_S 73_S 1.646 1.00
114_D 157_E 1.645 1.00
239_N 274_E 1.643 1.00
29_I 259_A 1.615 1.00
36_F 266_D 1.614 1.00
356_V 359_A 1.597 1.00
75_M 349_M 1.596 1.00
113_I 158_V 1.594 1.00
57_S 69_S 1.593 1.00
20_S 24_A 1.581 1.00
12_K 82_C 1.577 1.00
430_P 433_N 1.567 1.00
14_R 279_P 1.562 1.00
318_M 365_L 1.56 1.00
428_F 439_G 1.558 1.00
19_I 109_C 1.546 1.00
123_T 139_T 1.534 1.00
185_L 188_D 1.533 1.00
412_L 453_L 1.527 1.00
82_C 273_V 1.525 1.00
325_Y 331_L 1.517 1.00
139_T 158_V 1.512 1.00
77_F 86_L 1.481 1.00
316_A 373_I 1.474 1.00
173_I 183_Y 1.47 0.99
37_G 77_F 1.461 0.99
62_M 70_I 1.461 0.99
335_R 377_D 1.458 0.99
29_I 268_M 1.443 0.99
66_R 451_A 1.431 0.99
332_R 337_A 1.43 0.99
290_E 294_D 1.418 0.99
294_D 322_S 1.414 0.99
346_L 355_H 1.41 0.99
124_R 161_E 1.403 0.99
20_S 23_D 1.402 0.99
18_I 30_T 1.402 0.99
330_K 339_D 1.402 0.99
111_M 126_L 1.393 0.99
155_L 254_V 1.385 0.99
326_E 329_M 1.376 0.99
19_I 103_L 1.376 0.99
169_I 271_G 1.371 0.99
79_Y 270_D 1.369 0.99
330_K 341_V 1.364 0.99
150_D 153_E 1.359 0.99
202_Q 205_R 1.357 0.99
113_I 139_T 1.353 0.99
80_H 270_D 1.351 0.99
108_C 277_P 1.35 0.99
187_K 235_K 1.349 0.99
450_V 463_Y 1.347 0.99
227_L 230_E 1.347 0.99
139_T 252_T 1.345 0.99
62_M 451_A 1.334 0.99
114_D 146_R 1.327 0.99
60_M 71_T 1.326 0.99
138_L 276_A 1.32 0.99
295_K 381_Q 1.318 0.99
40_I 56_K 1.317 0.99
225_Q 229_D 1.312 0.99
33_V 269_L 1.311 0.99
78_P 81_D 1.303 0.99
131_R 137_I 1.301 0.98
80_H 274_E 1.297 0.98
450_V 454_K 1.295 0.98
415_G 419_L 1.288 0.98
155_L 168_P 1.283 0.98
142_N 259_A 1.274 0.98
78_P 83_L 1.267 0.98
304_Q 317_F 1.266 0.98
233_L 237_A 1.263 0.98
452_R 456_E 1.253 0.98
180_K 192_L 1.246 0.98
244_E 248_A 1.245 0.98
420_S 438_V 1.242 0.98
238_S 242_D 1.239 0.98
214_D 218_A 1.233 0.98
207_V 216_D 1.231 0.98
359_A 363_D 1.23 0.98
419_L 453_L 1.22 0.97
16_F 272_L 1.218 0.97
405_D 408_K 1.218 0.97
211_N 218_A 1.207 0.97
112_V 259_A 1.206 0.97
59_W 304_Q 1.205 0.97
117_K 157_E 1.199 0.97
188_D 207_V 1.195 0.97
87_L 106_V 1.195 0.97
483_E 486_K 1.192 0.97
225_Q 228_R 1.191 0.97
340_V 373_I 1.188 0.97
303_I 375_I 1.183 0.97
103_L 109_C 1.182 0.97
228_R 233_L 1.181 0.97
40_I 43_A 1.17 0.96
151_P 168_P 1.167 0.96
33_V 37_G 1.167 0.96
28_T 46_V 1.156 0.96
220_G 224_A 1.155 0.96
107_D 319_R 1.153 0.96
36_F 262_N 1.153 0.96
480_K 484_E 1.149 0.96
49_R 53_Q 1.148 0.96
100_Y 129_V 1.142 0.96
318_M 359_A 1.139 0.96
23_D 68_I 1.138 0.96
110_L 268_M 1.136 0.96
289_V 294_D 1.136 0.96
31_E 55_A 1.13 0.95
297_T 387_F 1.13 0.95
399_R 463_Y 1.127 0.95
100_Y 126_L 1.12 0.95
216_D 228_R 1.112 0.95
228_R 232_E 1.109 0.95
449_V 453_L 1.107 0.95
111_M 123_T 1.104 0.95
297_T 322_S 1.101 0.95
207_V 211_N 1.1 0.95
216_D 425_V 1.099 0.95
440_A 446_F 1.097 0.94
234_V 238_S 1.097 0.94
152_M 156_D 1.091 0.94
157_E 161_E 1.085 0.94
228_R 231_L 1.084 0.94
144_L 175_C 1.081 0.94
107_D 136_P 1.08 0.94
58_D 63_E 1.08 0.94
65_Q 455_S 1.079 0.94
174_G 181_G 1.079 0.94
114_D 120_E 1.079 0.94
174_G 179_F 1.078 0.94
292_S 324_K 1.077 0.94
144_L 179_F 1.076 0.94
13_R 85_N 1.075 0.94
189_E 203_E 1.075 0.94
130_T 137_I 1.07 0.93
111_M 139_T 1.068 0.93
80_H 273_V 1.068 0.93
336_T 374_Q 1.066 0.93
332_R 339_D 1.065 0.93
289_V 385_M 1.059 0.93
228_R 238_S 1.058 0.93
226_Q 231_L 1.057 0.93
83_L 347_T 1.056 0.93
324_K 360_Y 1.055 0.93
308_D 311_H 1.055 0.93
173_I 181_G 1.053 0.93
221_E 224_A 1.05 0.93
317_F 348_F 1.048 0.92
338_K 372_T 1.046 0.92
234_V 242_D 1.045 0.92
408_K 411_Q 1.044 0.92
296_F 365_L 1.043 0.92
435_D 466_V 1.043 0.92
156_D 159_E 1.04 0.92
318_M 325_Y 1.039 0.92
49_R 52_N 1.039 0.92
119_V 162_L 1.038 0.92
112_V 142_N 1.038 0.92
235_K 239_N 1.035 0.92
359_A 367_L 1.034 0.92
93_E 125_K 1.032 0.92
92_H 101_R 1.032 0.92
99_T 126_L 1.03 0.92
258_T 261_G 1.029 0.92
303_I 316_A 1.021 0.91
221_E 225_Q 1.02 0.91
240_E 244_E 1.02 0.91
174_G 180_K 1.016 0.91
216_D 220_G 1.015 0.91
207_V 214_D 1.014 0.91
243_K 247_L 1.014 0.91
360_Y 363_D 1.013 0.91
141_M 154_L 1.011 0.91
393_F 396_E 1.008 0.90
510_N 513_L 1.006 0.90
223_L 226_Q 1.006 0.90
70_I 448_V 1.005 0.90
272_L 276_A 1.004 0.90
43_A 262_N 1.003 0.90
333_Q 373_I 1.002 0.90
152_M 171_W 1.001 0.90
315_V 366_G 1.001 0.90
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2h5eA211000.227Contact Map0.671
3vqtA40.99811000.239Contact Map0.706
3tr5A40.99621000.239Contact Map0.666
4kiyV10.97161000.332Contact Map0.743
1darA10.97351000.338Contact Map0.738
4fn5A10.97351000.344Contact Map0.748
2xexA20.97161000.348Contact Map0.746
3j25A10.89221000.385Contact Map0.084
2dy1A10.94711000.387Contact Map0.684
1n0uA10.95091000.418Contact Map0.645
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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