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OPENSEQ.org
LEPA - Elongation factor 4
UniProt: P60785 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10529
Length: 599 (595)
Sequences: 3328
Seq/Len: 5.59
LEPA
Paralog alert: 0.93 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: EFG LEPA RF3 TYPA
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
250_L 256_G 5.405 1.00
145_E 149_D 4.303 1.00
129_V 163_A 4.129 1.00
41_L 59_V 3.625 1.00
161_C 172_V 3.622 1.00
192_Q 214_N 3.344 1.00
159_V 175_R 3.228 1.00
405_E 471_N 3.163 1.00
210_I 258_L 3.157 1.00
103_D 106_Q 3.136 1.00
105_G 135_L 3.128 1.00
102_V 112_T 3.112 1.00
221_D 276_L 3.098 1.00
186_D 189_G 3.085 1.00
410_L 466_A 3.025 1.00
398_E 477_A 2.879 1.00
405_E 442_T 2.854 1.00
444_E 473_K 2.825 1.00
139_D 142_R 2.814 1.00
175_R 179_D 2.811 1.00
324_L 335_G 2.758 1.00
218_R 249_E 2.707 1.00
216_T 249_E 2.692 1.00
479_D 501_R 2.581 1.00
421_L 454_F 2.529 1.00
313_A 355_L 2.502 1.00
129_V 161_C 2.49 1.00
10_I 100_L 2.466 1.00
377_T 477_A 2.444 1.00
410_L 418_V 2.416 1.00
212_I 250_L 2.4 1.00
418_V 462_S 2.393 1.00
31_L 40_V 2.39 1.00
405_E 473_K 2.381 1.00
142_R 146_E 2.356 1.00
64_K 70_T 2.352 1.00
296_Y 334_L 2.278 1.00
219_K 248_T 2.274 1.00
230_Q 267_G 2.247 1.00
428_V 476_Q 2.243 1.00
206_V 319_L 2.239 1.00
21_S 75_F 2.213 1.00
224_K 280_P 2.193 1.00
27_I 170_Q 2.175 1.00
408_M 422_C 2.172 1.00
430_T 444_E 2.166 1.00
32_S 35_E 2.151 1.00
373_E 400_R 2.124 1.00
144_A 160_R 2.072 1.00
444_E 474_R 2.025 1.00
487_I 583_L 2.013 1.00
444_E 476_Q 2.009 1.00
218_R 221_D 2.007 1.00
375_E 400_R 2 1.00
128_P 147_I 1.996 1.00
375_E 398_E 1.985 1.00
226_M 275_T 1.984 1.00
296_Y 336_F 1.975 1.00
103_D 143_V 1.96 1.00
216_T 251_K 1.954 1.00
302_V 362_D 1.95 1.00
236_R 246_D 1.945 1.00
199_W 209_L 1.937 1.00
434_Y 439_V 1.925 1.00
577_Q 582_E 1.91 1.00
191_L 223_V 1.908 1.00
418_V 458_L 1.906 1.00
7_F 73_L 1.856 1.00
223_V 234_A 1.855 1.00
276_L 279_N 1.845 1.00
210_I 256_G 1.833 1.00
422_C 427_G 1.823 1.00
578_I 583_L 1.799 1.00
143_V 146_E 1.791 1.00
224_K 231_T 1.764 1.00
195_I 208_S 1.761 1.00
112_T 128_P 1.755 1.00
104_A 133_I 1.752 1.00
115_N 118_T 1.75 1.00
483_V 499_T 1.739 1.00
433_V 442_T 1.731 1.00
302_V 364_I 1.729 1.00
324_L 351_I 1.729 1.00
217_L 234_A 1.72 1.00
373_E 381_V 1.711 1.00
239_I 247_R 1.701 1.00
66_S 170_Q 1.683 1.00
27_I 169_V 1.679 1.00
212_I 256_G 1.674 1.00
222_K 231_T 1.671 1.00
187_P 216_T 1.671 1.00
372_Y 448_A 1.665 1.00
102_V 147_I 1.656 1.00
375_E 381_V 1.656 1.00
72_Q 254_E 1.655 1.00
462_S 466_A 1.65 1.00
104_A 138_A 1.647 1.00
191_L 212_I 1.626 0.99
3_N 69_E 1.621 0.99
208_S 268_A 1.617 0.99
103_D 146_E 1.597 0.99
505_Q 509_R 1.577 0.99
457_R 461_T 1.572 0.99
523_Q 545_L 1.571 0.99
292_K 325_F 1.55 0.99
479_D 502_D 1.546 0.99
127_V 180_I 1.541 0.99
415_L 441_L 1.54 0.99
39_Q 48_R 1.539 0.99
562_K 566_Q 1.535 0.99
28_C 61_L 1.534 0.99
224_K 229_G 1.532 0.99
300_F 364_I 1.524 0.99
144_A 158_A 1.521 0.99
3_N 71_Y 1.52 0.99
86_Y 323_S 1.501 0.99
318_S 324_L 1.5 0.99
426_R 449_E 1.496 0.99
174_E 178_R 1.496 0.99
6_N 74_N 1.49 0.99
49_E 357_R 1.483 0.99
46_L 54_I 1.46 0.99
422_C 445_I 1.455 0.98
432_M 439_V 1.452 0.98
62_D 70_T 1.429 0.98
409_L 440_A 1.427 0.98
356_E 362_D 1.426 0.98
10_I 92_L 1.409 0.98
415_L 432_M 1.401 0.98
428_V 444_E 1.398 0.98
102_V 128_P 1.392 0.98
113_L 465_Y 1.379 0.98
512_V 540_S 1.378 0.98
318_S 326_Y 1.373 0.98
20_L 163_A 1.368 0.98
3_N 70_T 1.366 0.97
553_C 558_I 1.359 0.97
228_T 269_P 1.351 0.97
353_E 357_R 1.344 0.97
4_I 254_E 1.34 0.97
210_I 250_L 1.333 0.97
106_Q 146_E 1.33 0.97
422_C 443_Y 1.328 0.97
406_C 451_V 1.327 0.97
472_F 475_F 1.326 0.97
103_D 135_L 1.319 0.97
407_H 440_A 1.316 0.97
168_G 171_D 1.316 0.97
404_A 450_V 1.305 0.96
525_D 543_K 1.305 0.96
26_Q 33_D 1.299 0.96
148_E 154_D 1.296 0.96
250_L 258_L 1.296 0.96
109_E 409_L 1.293 0.96
147_I 158_A 1.286 0.96
553_C 561_K 1.285 0.96
548_N 553_C 1.283 0.96
165_T 169_V 1.283 0.96
508_G 530_A 1.28 0.96
161_C 168_G 1.279 0.96
63_Y 174_E 1.275 0.96
354_R 358_E 1.272 0.96
89_S 115_N 1.254 0.95
62_D 72_Q 1.242 0.95
388_S 392_A 1.239 0.95
239_I 250_L 1.239 0.95
65_A 174_E 1.236 0.95
352_Q 365_T 1.232 0.95
381_V 400_R 1.231 0.95
189_G 214_N 1.231 0.95
210_I 217_L 1.228 0.95
329_E 373_E 1.227 0.94
407_H 442_T 1.216 0.94
323_S 351_I 1.215 0.94
483_V 497_L 1.21 0.94
200_F 316_K 1.21 0.94
20_L 169_V 1.206 0.94
37_E 48_R 1.205 0.94
172_V 176_L 1.203 0.94
389_K 392_A 1.196 0.93
100_L 128_P 1.195 0.93
375_E 397_Y 1.193 0.93
65_A 178_R 1.191 0.93
559_S 566_Q 1.189 0.93
130_L 143_V 1.186 0.93
4_I 72_Q 1.184 0.93
227_S 272_D 1.178 0.93
514_K 517_D 1.177 0.93
403_I 476_Q 1.175 0.93
377_T 397_Y 1.174 0.93
45_D 357_R 1.173 0.93
30_G 61_L 1.169 0.92
231_T 277_A 1.166 0.92
89_S 118_T 1.164 0.92
10_I 98_A 1.163 0.92
384_V 392_A 1.163 0.92
522_Q 527_A 1.161 0.92
486_L 531_A 1.16 0.92
195_I 268_A 1.159 0.92
145_E 148_E 1.159 0.92
133_I 167_V 1.157 0.92
186_D 216_T 1.15 0.92
335_G 351_I 1.146 0.91
574_R 585_Q 1.146 0.91
226_M 284_A 1.145 0.91
382_I 394_N 1.14 0.91
100_L 112_T 1.14 0.91
545_L 553_C 1.139 0.91
377_T 398_E 1.136 0.91
451_V 470_Y 1.135 0.91
458_L 462_S 1.135 0.91
406_C 468_L 1.134 0.91
141_E 145_E 1.133 0.91
187_P 251_K 1.131 0.91
506_N 509_R 1.126 0.90
310_F 355_L 1.126 0.90
9_I 21_S 1.125 0.90
528_I 542_V 1.123 0.90
527_A 544_Q 1.122 0.90
106_Q 143_V 1.121 0.90
419_I 429_Q 1.121 0.90
386_S 390_L 1.121 0.90
168_G 172_V 1.121 0.90
570_E 573_K 1.119 0.90
405_E 430_T 1.115 0.90
61_L 75_F 1.114 0.90
406_C 445_I 1.11 0.90
246_D 257_W 1.107 0.89
24_I 61_L 1.103 0.89
187_P 252_C 1.102 0.89
225_V 274_L 1.099 0.89
405_E 444_E 1.099 0.89
11_A 15_H 1.097 0.89
43_S 57_Q 1.093 0.89
232_Y 268_A 1.092 0.89
225_V 268_A 1.09 0.88
376_T 382_I 1.09 0.88
513_E 540_S 1.088 0.88
71_Y 177_V 1.086 0.88
34_R 240_F 1.082 0.88
240_F 327_E 1.082 0.88
236_R 257_W 1.071 0.87
159_V 172_V 1.071 0.87
385_D 389_K 1.069 0.87
226_M 281_A 1.066 0.87
21_S 41_L 1.062 0.87
41_L 53_T 1.062 0.87
351_I 355_L 1.062 0.87
222_K 233_N 1.059 0.86
490_E 580_N 1.059 0.86
11_A 14_D 1.054 0.86
46_L 353_E 1.05 0.86
99_L 172_V 1.049 0.86
392_A 395_N 1.049 0.86
100_L 115_N 1.044 0.85
258_L 274_L 1.04 0.85
39_Q 59_V 1.039 0.85
5_R 183_P 1.038 0.85
550_L 553_C 1.032 0.84
545_L 548_N 1.031 0.84
406_C 443_Y 1.027 0.84
219_K 246_D 1.027 0.84
43_S 55_K 1.024 0.84
553_C 562_K 1.022 0.84
501_R 532_I 1.021 0.84
218_R 248_T 1.02 0.83
101_V 163_A 1.019 0.83
269_P 290_K 1.017 0.83
318_S 335_G 1.014 0.83
376_T 395_N 1.011 0.83
431_N 442_T 1.011 0.83
531_A 536_I 1.007 0.82
18_S 21_S 1.006 0.82
509_R 540_S 1.002 0.82
140_P 143_V 1.001 0.82
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3cb4D611000.162Contact Map0.699
2yweA10.99331000.167Contact Map0.725
3j25A10.80471000.499Contact Map0.104
4kiyV10.79971000.501Contact Map0.739
1darA10.79131000.503Contact Map0.733
4fn5A10.79971000.503Contact Map0.723
2xexA20.79471000.509Contact Map0.741
2dy1A10.7931000.529Contact Map0.688
1n0uA10.84141000.54Contact Map0.7
3j3az10.83141000.541Contact Map0.591
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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