May 4, 2021 - We are working on upgrading the webserver, some pages may not work.
OPENSEQ.org
YAGF - Uncharacterized protein YagF
UniProt: P77596 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13345
Length: 655 (568)
Sequences: 1523
Seq/Len: 2.68
YAGF
Paralog alert: 0.49 [within 20: 0.00] - ratio of genomes with paralogs
Cluster includes: EDD ILVD YAGF YJHG
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
470_T 568_E 4.813 1.00
446_P 557_P 4.18 1.00
291_L 296_I 4.082 1.00
155_A 281_D 4.055 1.00
560_K 599_L 4.008 1.00
297_E 379_H 3.753 1.00
560_K 603_Q 3.458 1.00
294_K 379_H 3.412 1.00
182_I 277_V 3.291 1.00
302_I 369_M 3.135 1.00
449_S 576_L 3.003 1.00
373_R 380_L 2.925 1.00
493_Q 527_T 2.854 1.00
380_L 391_G 2.851 1.00
178_D 289_D 2.786 1.00
174_A 291_L 2.74 1.00
444_I 561_L 2.737 1.00
561_L 578_G 2.734 1.00
286_T 289_D 2.608 1.00
258_L 356_T 2.601 1.00
474_R 563_D 2.575 1.00
382_A 390_V 2.571 1.00
241_T 308_G 2.558 1.00
294_K 381_D 2.448 1.00
474_R 495_D 2.436 1.00
304_A 336_I 2.399 1.00
386_T 393_N 2.357 1.00
214_S 217_E 2.338 1.00
64_V 155_A 2.326 1.00
158_G 181_T 2.296 1.00
155_A 277_V 2.293 1.00
470_T 566_I 2.271 1.00
581_N 593_E 2.259 1.00
334_T 338_R 2.231 1.00
170_M 251_L 2.221 1.00
300_M 333_W 2.187 1.00
276_A 279_E 2.165 1.00
485_A 491_I 2.142 1.00
251_L 291_L 2.121 1.00
505_S 554_A 2.114 1.00
291_L 295_A 2.093 1.00
301_V 340_V 2.086 1.00
298_N 382_A 2.065 1.00
178_D 288_R 2.051 1.00
477_V 501_G 2.051 1.00
389_T 392_E 2.032 1.00
246_A 252_A 1.984 0.99
182_I 276_A 1.969 0.99
58_K 61_G 1.945 0.99
64_V 281_D 1.944 0.99
570_A 573_R 1.926 0.99
68_G 112_A 1.883 0.99
86_H 117_D 1.878 0.99
473_V 561_L 1.855 0.99
66_I 103_I 1.852 0.99
388_Q 392_E 1.819 0.99
472_R 566_I 1.799 0.99
561_L 567_I 1.793 0.99
297_E 335_R 1.785 0.99
568_E 577_T 1.782 0.99
334_T 574_L 1.779 0.99
599_L 603_Q 1.743 0.98
295_A 316_I 1.724 0.98
157_I 274_A 1.714 0.98
369_M 390_V 1.713 0.98
476_F 490_E 1.704 0.98
331_E 441_T 1.698 0.98
301_V 336_I 1.692 0.98
475_V 561_L 1.682 0.98
477_V 481_Q 1.672 0.98
170_M 319_I 1.666 0.98
102_E 274_A 1.661 0.97
447_E 632_W 1.647 0.97
443_N 577_T 1.64 0.97
178_D 286_T 1.637 0.97
104_T 110_P 1.624 0.97
280_L 285_I 1.616 0.97
208_F 213_L 1.614 0.97
248_A 302_I 1.596 0.97
531_I 548_V 1.594 0.97
558_I 561_L 1.587 0.96
359_A 368_V 1.585 0.96
207_R 212_E 1.582 0.96
489_E 522_I 1.581 0.96
557_P 599_L 1.566 0.96
182_I 385_V 1.564 0.96
174_A 251_L 1.552 0.96
474_R 485_A 1.547 0.96
301_V 339_K 1.54 0.96
484_K 488_R 1.529 0.95
371_H 374_D 1.526 0.95
376_G 380_L 1.514 0.95
562_R 605_H 1.514 0.95
444_I 500_I 1.506 0.95
297_E 381_D 1.495 0.95
466_V 469_H 1.49 0.94
67_I 156_V 1.487 0.94
272_Q 275_R 1.487 0.94
172_A 287_T 1.481 0.94
158_G 183_L 1.474 0.94
601_R 604_T 1.461 0.94
191_P 357_V 1.458 0.94
424_P 427_K 1.446 0.93
190_L 263_Q 1.443 0.93
438_C 572_D 1.442 0.93
279_E 282_S 1.436 0.93
313_L 317_P 1.428 0.93
378_L 390_V 1.425 0.92
228_S 264_A 1.424 0.92
389_T 393_N 1.419 0.92
476_F 485_A 1.409 0.92
213_L 217_E 1.408 0.92
290_I 295_A 1.401 0.92
172_A 177_H 1.399 0.92
174_A 296_I 1.396 0.91
65_L 147_I 1.394 0.91
373_R 391_G 1.387 0.91
369_M 394_L 1.384 0.91
457_D 526_K 1.384 0.91
293_D 297_E 1.384 0.91
193_T 221_L 1.381 0.91
473_V 567_I 1.37 0.90
469_H 472_R 1.367 0.90
477_V 562_R 1.36 0.90
495_D 563_D 1.357 0.90
220_E 224_R 1.345 0.89
143_F 172_A 1.337 0.89
241_T 312_L 1.336 0.89
388_Q 393_N 1.332 0.89
296_I 319_I 1.327 0.88
208_F 215_L 1.327 0.88
478_S 481_Q 1.32 0.88
190_L 262_G 1.318 0.88
568_E 579_S 1.311 0.88
96_M 159_V 1.311 0.88
125_G 514_Q 1.31 0.88
70_Q 93_G 1.302 0.87
438_C 574_L 1.296 0.87
230_G 507_T 1.293 0.87
130_F 510_E 1.284 0.86
468_H 568_E 1.283 0.86
451_I 571_V 1.281 0.86
185_P 256_S 1.275 0.86
56_A 223_C 1.27 0.85
276_A 283_R 1.268 0.85
252_A 256_S 1.26 0.85
184_V 273_S 1.259 0.85
335_R 339_K 1.256 0.84
475_V 578_G 1.255 0.84
477_V 500_I 1.248 0.84
173_L 181_T 1.237 0.83
147_I 156_V 1.237 0.83
231_G 507_T 1.236 0.83
300_M 327_I 1.236 0.83
101_K 104_T 1.232 0.83
293_D 335_R 1.228 0.83
50_A 223_C 1.225 0.82
498_V 578_G 1.213 0.82
293_D 326_T 1.213 0.82
157_I 277_V 1.206 0.81
190_L 228_S 1.2 0.81
420_D 427_K 1.2 0.81
578_G 590_L 1.197 0.80
382_A 392_E 1.196 0.80
66_I 156_V 1.191 0.80
178_D 287_T 1.191 0.80
265_V 360_F 1.19 0.80
118_P 132_S 1.188 0.80
100_A 274_A 1.188 0.80
64_V 103_I 1.187 0.80
124_Q 237_G 1.186 0.79
54_W 109_I 1.186 0.79
291_L 323_A 1.183 0.79
174_A 323_A 1.181 0.79
582_F 599_L 1.175 0.79
331_E 335_R 1.175 0.79
93_G 97_Q 1.173 0.78
108_G 278_S 1.17 0.78
118_P 149_S 1.167 0.78
190_L 260_P 1.166 0.78
468_H 570_A 1.162 0.78
486_I 515_L 1.162 0.78
88_G 119_C 1.161 0.77
50_A 56_A 1.152 0.77
56_A 219_A 1.149 0.76
63_E 111_F 1.149 0.76
89_H 188_A 1.145 0.76
124_Q 235_F 1.145 0.76
58_K 275_R 1.144 0.76
499_V 540_S 1.143 0.76
185_P 266_W 1.135 0.75
99_A 157_I 1.128 0.75
67_I 114_F 1.127 0.74
516_T 536_F 1.125 0.74
293_D 332_H 1.124 0.74
256_S 269_I 1.124 0.74
165_G 169_T 1.116 0.73
472_R 578_G 1.112 0.73
594_E 601_R 1.112 0.73
170_M 291_L 1.11 0.73
390_V 393_N 1.105 0.72
99_A 274_A 1.104 0.72
185_P 243_Q 1.101 0.72
382_A 391_G 1.098 0.72
481_Q 484_K 1.096 0.71
456_I 543_A 1.092 0.71
121_G 129_M 1.091 0.71
392_E 395_E 1.089 0.71
202_Q 205_G 1.086 0.70
566_I 579_S 1.082 0.70
251_L 292_S 1.08 0.70
562_R 594_E 1.079 0.70
520_K 525_G 1.076 0.69
306_F 359_A 1.076 0.69
267_L 271_R 1.07 0.69
296_I 300_M 1.07 0.69
205_G 507_T 1.067 0.69
85_Y 153_R 1.061 0.68
520_K 544_C 1.058 0.68
105_R 275_R 1.057 0.67
186_G 232_G 1.056 0.67
97_Q 101_K 1.055 0.67
303_H 312_L 1.054 0.67
65_L 156_V 1.053 0.67
296_I 316_I 1.046 0.66
439_F 450_V 1.043 0.66
448_G 631_T 1.041 0.66
143_F 369_M 1.039 0.66
349_N 455_A 1.037 0.65
54_W 111_F 1.037 0.65
172_A 179_L 1.037 0.65
624_L 628_S 1.034 0.65
236_L 306_F 1.031 0.65
197_D 357_V 1.031 0.65
294_K 297_E 1.029 0.64
491_I 495_D 1.028 0.64
480_A 510_E 1.024 0.64
475_V 563_D 1.023 0.64
585_T 597_R 1.022 0.64
86_H 146_L 1.019 0.63
292_S 295_A 1.019 0.63
474_R 488_R 1.018 0.63
248_A 369_M 1.017 0.63
474_R 492_V 1.014 0.63
277_V 577_T 1.014 0.63
217_E 220_E 1.011 0.62
314_L 317_P 1.007 0.62
126_T 151_P 1.003 0.62
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2gp4A20.89621000.197Contact Map0.601
2pcnA10.212274.10.983Contact Map0.42
1j3lA60.215371.60.983Contact Map0.446
1q5xA30.209262.70.984Contact Map0.462
3c8oA20.212260.90.985Contact Map0.442
1nxjA30.242749.60.986Contact Map0.392
1vi4A10.230544.80.986Contact Map0.367
1c41A100.224441.70.986Contact Map0.626
2obxA100.201535.20.987Contact Map0.524
3nojA10.317631.40.987Contact Map0.53
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
Page generated in 0.1015 seconds.