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OPENSEQ.org

GLSA1 - Glutaminase 1
UniProt: P77454 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13247
Length: 310 (304)
Sequences: 693
Seq/Len: 2.28

GLSA1
Paralog alert: 0.37 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: GLSA1 GLSA2
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
154_S 199_L 4.802 1.00
166_F 173_W 3.531 1.00
40_L 60_Y 3.516 1.00
149_E 204_E 3.342 1.00
145_Q 223_K 3.329 1.00
79_D 138_R 2.607 1.00
46_V 206_A 2.536 1.00
25_Q 28_D 2.491 1.00
71_C 208_L 2.444 1.00
165_N 193_T 2.432 1.00
42_A 202_T 2.395 1.00
217_V 224_R 2.376 1.00
13_Q 302_Q 2.313 1.00
89_I 123_T 2.127 1.00
61_R 204_E 2.069 0.99
46_V 52_V 1.957 0.99
92_D 245_G 1.926 0.99
41_A 295_M 1.873 0.99
256_L 268_A 1.867 0.99
59_D 203_I 1.861 0.99
246_R 284_D 1.824 0.98
54_S 58_S 1.811 0.98
52_V 206_A 1.802 0.98
27_A 30_I 1.794 0.98
16_T 19_H 1.743 0.98
44_A 203_I 1.699 0.97
72_T 139_I 1.69 0.97
209_G 267_L 1.686 0.97
121_I 193_T 1.663 0.97
27_A 262_V 1.647 0.96
8_Q 12_D 1.645 0.96
17_Q 298_S 1.643 0.96
171_I 174_L 1.631 0.96
162_Q 165_N 1.611 0.96
64_L 68_S 1.611 0.96
52_V 203_I 1.605 0.95
189_C 193_T 1.584 0.95
171_I 175_L 1.576 0.95
239_M 251_A 1.569 0.95
68_S 195_Q 1.55 0.94
67_I 265_G 1.526 0.94
48_C 214_A 1.498 0.93
142_I 146_L 1.486 0.92
74_A 211_T 1.474 0.92
280_S 291_R 1.472 0.92
143_Q 151_V 1.446 0.91
32_F 156_E 1.437 0.91
268_A 296_V 1.423 0.90
139_I 195_Q 1.418 0.90
278_A 292_G 1.418 0.90
253_R 297_A 1.417 0.90
166_F 176_Y 1.404 0.89
67_I 205_L 1.402 0.89
162_Q 193_T 1.396 0.89
123_T 185_A 1.372 0.88
144_Q 151_V 1.37 0.88
167_H 177_S 1.369 0.88
54_S 59_D 1.365 0.87
218_N 221_T 1.363 0.87
159_Q 163_T 1.348 0.87
15_Y 41_A 1.338 0.86
36_V 40_L 1.338 0.86
57_D 60_Y 1.336 0.86
29_Y 262_V 1.327 0.85
85_V 126_L 1.324 0.85
43_V 55_A 1.315 0.85
94_T 116_V 1.295 0.83
270_V 274_M 1.294 0.83
15_Y 55_A 1.289 0.83
122_A 180_Y 1.287 0.83
282_P 291_R 1.276 0.82
210_A 219_P 1.274 0.82
162_Q 190_D 1.257 0.81
104_L 111_P 1.242 0.80
243_L 258_G 1.227 0.79
27_A 34_A 1.225 0.78
20_S 129_A 1.223 0.78
92_D 95_G 1.216 0.78
121_I 162_Q 1.214 0.78
155_D 194_R 1.21 0.77
47_T 51_N 1.208 0.77
93_P 241_E 1.205 0.77
29_Y 33_L 1.201 0.77
205_L 277_A 1.2 0.76
32_F 35_N 1.189 0.76
294_K 298_S 1.185 0.75
93_P 245_G 1.181 0.75
74_A 146_L 1.179 0.75
256_L 300_A 1.177 0.74
15_Y 19_H 1.176 0.74
137_Q 141_H 1.174 0.74
32_F 160_S 1.164 0.73
258_G 268_A 1.162 0.73
30_I 33_L 1.149 0.72
44_A 54_S 1.147 0.72
93_P 104_L 1.143 0.71
61_R 201_N 1.142 0.71
294_K 297_A 1.141 0.71
11_V 247_S 1.139 0.71
63_A 157_V 1.136 0.71
121_I 189_C 1.134 0.70
42_A 57_D 1.132 0.70
184_D 187_E 1.132 0.70
31_P 164_T 1.131 0.70
246_R 293_Q 1.128 0.70
81_G 85_V 1.122 0.69
72_T 195_Q 1.121 0.69
123_T 202_T 1.116 0.69
208_L 212_L 1.103 0.67
105_E 109_G 1.103 0.67
105_E 240_M 1.102 0.67
298_S 302_Q 1.09 0.66
181_L 195_Q 1.087 0.66
165_N 189_C 1.085 0.66
61_R 181_L 1.075 0.65
31_P 160_S 1.074 0.64
27_A 33_L 1.065 0.63
12_D 16_T 1.065 0.63
161_E 262_V 1.065 0.63
35_N 156_E 1.06 0.63
218_N 225_V 1.055 0.62
269_V 275_G 1.053 0.62
10_A 302_Q 1.051 0.62
121_I 165_N 1.05 0.62
26_N 38_G 1.047 0.62
79_D 135_R 1.047 0.62
73_L 86_Q 1.044 0.61
43_V 295_M 1.044 0.61
32_F 241_E 1.042 0.61
111_P 161_E 1.035 0.60
26_N 34_A 1.03 0.60
78_E 230_N 1.029 0.60
271_P 280_S 1.027 0.59
270_V 273_V 1.027 0.59
144_Q 149_E 1.025 0.59
40_L 57_D 1.02 0.59
63_A 115_L 1.018 0.58
207_T 211_T 1.008 0.57
155_D 159_Q 1.008 0.57
30_I 161_E 1.007 0.57
17_Q 221_T 1.007 0.57
76_A 254_V 1.003 0.57
46_V 207_T 1.003 0.57
251_A 270_V 1.001 0.56
125_S 158_N 1.001 0.56
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1u60A411000.018Contact Map0.699
2pbyA40.98711000.026Contact Map0.735
3uo9A40.99351000.039Contact Map0.712
4bqmA20.99351000.04Contact Map0.603
1mkiA20.99031000.043Contact Map0.628
3agdA20.99031000.069Contact Map0.649
1ghpA10.751698.70.916Contact Map0.501
4dxbA20.838798.40.925Contact Map0.517
1e25A10.787198.30.927Contact Map0.458
2wuqA20.880698.30.928Contact Map0.528

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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