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OPENSEQ.org
FLGE - Flagellar hook protein FlgE
UniProt: P75937 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG14269
Length: 402 (402)
Sequences: 913
Seq/Len: 2.27
FLGE
Paralog alert: 0.21 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: FLGE FLGF FLGG
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
321_N 324_G 3.971 1.00
94_Y 319_F 3.612 1.00
328_E 334_S 3.56 1.00
326_A 336_T 3.549 1.00
231_N 235_T 3.453 1.00
85_L 95_S 3.367 1.00
137_I 312_G 3.343 1.00
107_N 138_S 2.877 1.00
75_V 314_I 2.866 1.00
301_G 311_L 2.734 1.00
150_T 281_N 2.637 1.00
117_T 136_N 2.583 1.00
6_A 387_T 2.562 1.00
228_L 236_L 2.501 1.00
75_V 355_L 2.488 1.00
84_R 317_A 2.356 1.00
12_A 52_V 2.353 1.00
107_N 115_Q 2.322 1.00
345_G 352_F 2.288 1.00
75_V 293_I 2.272 1.00
289_V 304_S 2.237 1.00
24_I 367_L 2.186 1.00
115_Q 136_N 2.184 1.00
84_R 117_T 2.183 1.00
288_L 301_G 2.17 1.00
230_F 234_G 2.153 1.00
108_L 116_L 2.021 0.99
386_Q 389_K 2.009 0.99
77_I 316_L 1.996 0.99
16_N 377_A 1.995 0.99
21_G 387_T 1.977 0.99
110_N 114_L 1.973 0.99
75_V 116_L 1.948 0.99
75_V 357_N 1.86 0.99
313_Q 346_T 1.856 0.99
149_T 280_Q 1.845 0.98
386_Q 397_T 1.795 0.98
356_T 359_A 1.791 0.98
77_I 353_G 1.773 0.98
317_A 344_L 1.74 0.98
94_Y 342_A 1.713 0.97
66_T 76_A 1.679 0.97
66_T 359_A 1.662 0.96
392_D 395_L 1.64 0.96
157_N 160_S 1.618 0.96
151_T 281_N 1.61 0.96
13_A 380_N 1.588 0.95
377_A 394_I 1.582 0.95
206_N 268_Q 1.564 0.95
201_V 211_Y 1.541 0.94
85_L 93_F 1.534 0.94
388_I 392_D 1.52 0.93
1_M 402_R 1.515 0.93
171_S 174_N 1.488 0.92
27_S 367_L 1.45 0.91
298_T 310_L 1.435 0.91
19_V 32_F 1.406 0.89
18_D 50_L 1.402 0.89
69_T 74_D 1.402 0.89
59_Q 333_W 1.395 0.89
103_D 109_V 1.379 0.88
328_E 332_V 1.371 0.88
319_F 342_A 1.355 0.87
17_L 394_I 1.352 0.87
116_L 299_V 1.346 0.86
20_I 374_M 1.34 0.86
231_N 237_V 1.334 0.86
147_K 283_Y 1.333 0.86
286_G 304_S 1.327 0.85
61_F 82_F 1.326 0.85
16_N 373_N 1.321 0.85
294_N 298_T 1.319 0.85
379_R 392_D 1.319 0.85
123_G 126_P 1.31 0.84
294_N 300_V 1.305 0.84
395_L 399_V 1.301 0.84
32_F 35_G 1.3 0.84
86_V 117_T 1.293 0.83
371_L 397_T 1.284 0.83
22_N 41_D 1.281 0.82
293_I 314_I 1.279 0.82
17_L 374_M 1.275 0.82
209_Q 227_T 1.27 0.82
286_G 305_N 1.267 0.81
335_A 341_V 1.257 0.81
316_L 352_F 1.255 0.81
185_T 193_A 1.253 0.80
49_G 329_G 1.252 0.80
152_A 260_L 1.251 0.80
336_T 339_S 1.25 0.80
93_F 332_V 1.249 0.80
20_I 377_A 1.247 0.80
75_V 290_S 1.247 0.80
30_Y 360_L 1.244 0.80
122_T 127_T 1.237 0.79
116_L 314_I 1.229 0.79
290_S 302_N 1.221 0.78
178_Y 199_Y 1.221 0.78
103_D 107_N 1.219 0.78
36_T 58_T 1.217 0.78
32_F 393_Q 1.206 0.77
19_V 49_G 1.201 0.76
228_L 244_N 1.199 0.76
22_N 49_G 1.197 0.76
311_L 314_I 1.195 0.76
326_A 334_S 1.185 0.75
300_V 310_L 1.184 0.75
198_V 210_V 1.18 0.75
160_S 192_N 1.176 0.74
41_D 329_G 1.175 0.74
19_V 37_A 1.172 0.74
49_G 61_F 1.168 0.74
17_L 27_S 1.167 0.73
5_Q 395_L 1.163 0.73
67_T 362_A 1.155 0.72
371_L 389_K 1.153 0.72
202_K 208_W 1.147 0.72
389_K 392_D 1.142 0.71
300_V 308_T 1.142 0.71
114_L 332_V 1.132 0.70
373_N 390_T 1.125 0.69
68_N 358_G 1.116 0.69
71_R 74_D 1.115 0.69
366_D 369_K 1.111 0.68
27_S 386_Q 1.105 0.68
6_A 392_D 1.1 0.67
121_A 128_I 1.09 0.66
200_F 379_R 1.088 0.66
41_D 49_G 1.083 0.65
15_T 54_V 1.082 0.65
187_F 191_G 1.079 0.65
41_D 61_F 1.078 0.65
97_N 331_N 1.077 0.65
202_K 206_N 1.077 0.65
27_S 380_N 1.077 0.65
381_Y 387_T 1.077 0.65
374_M 399_V 1.076 0.64
316_L 355_L 1.075 0.64
318_N 352_F 1.074 0.64
16_N 395_L 1.071 0.64
381_Y 385_A 1.063 0.63
7_V 13_A 1.063 0.63
30_Y 97_N 1.062 0.63
152_A 196_M 1.059 0.63
200_F 228_L 1.058 0.63
319_F 335_A 1.056 0.62
369_K 373_N 1.051 0.62
99_Q 360_L 1.048 0.61
379_R 401_L 1.048 0.61
303_Y 389_K 1.048 0.61
160_S 190_Q 1.043 0.61
347_A 352_F 1.043 0.61
374_M 386_Q 1.039 0.60
314_I 355_L 1.032 0.60
38_S 371_L 1.032 0.60
368_S 376_V 1.03 0.59
24_I 374_M 1.029 0.59
147_K 150_T 1.028 0.59
285_P 398_L 1.027 0.59
79_Q 306_E 1.026 0.59
42_M 301_G 1.021 0.58
30_Y 99_Q 1.021 0.58
35_G 59_Q 1.02 0.58
3_F 333_W 1.02 0.58
186_V 194_H 1.019 0.58
10_L 21_G 1.018 0.58
189_S 206_N 1.018 0.58
350_G 370_E 1.017 0.58
188_D 194_H 1.016 0.58
76_A 359_A 1.014 0.58
22_N 324_G 1.012 0.57
386_Q 390_T 1.011 0.57
374_M 398_L 1.006 0.57
293_I 299_V 1.005 0.57
10_L 401_L 1.004 0.57
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3a69A10.99251000.175Contact Map0.657
1wlgA20.73631000.432Contact Map0.811
1oryB10.134390.979Contact Map0.21
4e45B60.10955.80.98Contact Map0.219
2m4eA10.1022.70.983Contact Map0.343
3ne8A10.14932.30.984Contact Map0.194
3czxA40.14681.90.985Contact Map0.155
2wzr310.15921.70.985Contact Map0
1d2dA10.08211.60.985Contact Map0.191
1m11310.15671.50.986Contact Map0
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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