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FIEF - Ferrous-iron efflux pump FieF
UniProt: P69380 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11873
Length: 300 (284)
Sequences: 4842
Seq/Len: 17.05
FIEF
Paralog alert: 0.76 [within 20: 0.03] - ratio of genomes with paralogs
Cluster includes: FIEF ZITB
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
177_R 181_L 3.663 1.00
232_H 248_H 2.987 1.00
247_I 282_I 2.796 1.00
98_F 184_L 2.668 1.00
192_Y 196_R 2.591 1.00
30_A 120_V 2.551 1.00
222_V 247_I 2.549 1.00
222_V 267_V 2.427 1.00
203_Q 208_R 2.371 1.00
68_D 75_H 2.3 1.00
221_I 274_R 2.192 1.00
71_H 75_H 2.187 1.00
249_L 267_V 2.164 1.00
261_H 283_H 2.112 1.00
87_S 198_G 2.064 1.00
78_A 206_L 2.062 1.00
28_I 43_L 2.046 1.00
219_I 223_T 2.008 1.00
31_W 40_L 2.006 1.00
221_I 275_F 1.989 1.00
119_I 123_V 1.956 1.00
99_L 103_Q 1.909 1.00
256_P 259_Q 1.891 1.00
170_L 178_A 1.889 1.00
139_V 148_R 1.882 1.00
97_L 183_A 1.836 1.00
225_W 270_A 1.8 1.00
259_Q 262_M 1.759 1.00
199_Y 203_Q 1.758 1.00
217_E 274_R 1.749 1.00
225_W 267_V 1.748 1.00
269_Q 273_R 1.748 1.00
216_Q 220_D 1.734 1.00
229_S 252_E 1.728 1.00
122_I 126_I 1.665 1.00
77_K 207_D 1.665 1.00
188_I 192_Y 1.664 1.00
184_L 188_I 1.641 1.00
118_V 165_L 1.638 1.00
45_D 153_H 1.638 1.00
19_M 127_C 1.586 1.00
98_F 187_G 1.572 1.00
220_D 224_S 1.57 1.00
54_L 58_L 1.558 1.00
221_I 270_A 1.551 1.00
122_I 165_L 1.538 1.00
27_K 46_S 1.528 1.00
251_M 257_L 1.527 1.00
245_I 278_S 1.516 1.00
28_I 32_W 1.509 1.00
29_F 33_Y 1.499 1.00
59_V 82_A 1.487 1.00
148_R 200_E 1.487 1.00
68_D 71_H 1.468 1.00
215_R 236_T 1.459 1.00
169_G 173_Y 1.444 1.00
163_A 182_F 1.44 1.00
14_I 18_A 1.428 1.00
73_F 246_Q 1.416 1.00
103_Q 107_S 1.368 0.99
232_H 285_D 1.365 0.99
45_D 49_D 1.354 0.99
230_G 250_E 1.35 0.99
150_D 153_H 1.348 0.99
266_Q 270_A 1.337 0.99
23_L 127_C 1.334 0.99
42_A 161_N 1.334 0.99
14_I 57_L 1.334 0.99
236_T 245_I 1.333 0.99
21_S 50_I 1.331 0.99
95_L 99_L 1.317 0.99
257_L 260_A 1.314 0.99
101_G 183_A 1.311 0.99
70_N 75_H 1.301 0.99
105_L 177_R 1.297 0.99
118_V 168_L 1.289 0.99
115_G 119_I 1.286 0.99
71_H 203_Q 1.281 0.99
102_I 106_I 1.273 0.99
253_D 258_V 1.268 0.99
10_S 64_L 1.267 0.99
207_D 244_F 1.266 0.99
37_V 101_G 1.263 0.99
115_G 172_W 1.261 0.99
225_W 266_Q 1.243 0.98
218_I 236_T 1.243 0.98
210_L 236_T 1.24 0.98
61_R 64_L 1.239 0.98
195_L 199_Y 1.237 0.98
31_W 43_L 1.23 0.98
262_M 266_Q 1.23 0.98
265_D 269_Q 1.224 0.98
80_S 204_S 1.219 0.98
270_A 274_R 1.215 0.98
177_R 180_A 1.212 0.98
225_W 263_V 1.212 0.98
81_L 206_L 1.211 0.98
214_E 217_E 1.207 0.98
94_A 191_L 1.197 0.98
100_T 103_Q 1.196 0.98
215_R 219_I 1.196 0.98
21_S 25_L 1.193 0.98
133_S 137_W 1.192 0.98
169_G 172_W 1.187 0.98
108_P 111_M 1.187 0.98
56_N 149_A 1.176 0.98
62_Y 78_A 1.173 0.98
23_L 27_K 1.162 0.97
42_A 45_D 1.159 0.97
24_L 28_I 1.148 0.97
136_R 140_R 1.147 0.97
258_V 262_M 1.145 0.97
271_I 278_S 1.139 0.97
118_V 172_W 1.138 0.97
223_T 230_G 1.118 0.96
28_I 40_L 1.11 0.96
10_S 61_R 1.107 0.96
137_W 141_R 1.104 0.96
136_R 151_M 1.103 0.96
221_I 225_W 1.102 0.96
268_E 283_H 1.085 0.96
167_A 182_F 1.08 0.95
181_L 185_G 1.079 0.95
72_S 209_A 1.079 0.95
17_T 50_I 1.07 0.95
162_G 166_L 1.069 0.95
25_L 29_F 1.067 0.95
91_S 194_A 1.067 0.95
129_I 133_S 1.063 0.95
211_P 214_E 1.061 0.95
264_A 283_H 1.06 0.95
18_A 22_L 1.058 0.95
216_Q 219_I 1.055 0.95
91_S 195_L 1.054 0.95
96_F 99_L 1.054 0.95
118_V 169_G 1.051 0.94
260_A 284_Q 1.05 0.94
24_L 47_L 1.049 0.94
130_I 133_S 1.048 0.94
235_R 279_D 1.047 0.94
79_E 144_S 1.044 0.94
251_M 263_V 1.041 0.94
19_M 131_L 1.037 0.94
268_E 272_L 1.037 0.94
217_E 220_D 1.031 0.94
204_S 238_Q 1.028 0.94
31_W 34_T 1.026 0.94
24_L 43_L 1.024 0.93
232_H 250_E 1.022 0.93
126_I 130_I 1.021 0.93
265_D 268_E 1.019 0.93
73_F 237_R 1.017 0.93
217_E 221_I 1.015 0.93
207_D 237_R 1.009 0.93
196_R 199_Y 1.007 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3j1zP20.99331000.107Contact Map0.587
3h90A40.94331000.128Contact Map0.641
3bypA20.306799.60.755Contact Map0.73
2zztA10.313399.60.77Contact Map0.562
1ji6A10.413334.70.952Contact Map0.362
1dlcA10.453320.10.957Contact Map0.256
2qkgA30.4517.70.958Contact Map0.38
1wh9A10.293316.80.959Contact Map0.501
3lnoA60.303316.50.959Contact Map0.583
2c9kA10.4067160.959Contact Map0.263
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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