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OPENSEQ.org
GSPF - Putative type II secretion system protein F
UniProt: P41441 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12885
Length: 398 (397)
Sequences: 2117
Seq/Len: 5.33
GSPF
Paralog alert: 0.38 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: GSPF HOFC
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
7_A 36_L 5.184 1.00
65_T 126_Y 4.644 1.00
19_I 27_A 4.06 1.00
202_L 206_I 3.969 1.00
238_R 241_N 3.574 1.00
21_A 27_A 3.285 1.00
104_A 117_H 3.249 1.00
111_L 115_L 2.966 1.00
27_A 41_I 2.938 1.00
203_S 381_F 2.744 1.00
304_A 318_L 2.658 1.00
243_H 299_Q 2.639 1.00
340_Q 343_T 2.527 1.00
289_T 302_A 2.465 1.00
82_L 105_I 2.45 1.00
190_T 212_D 2.276 1.00
64_F 98_L 2.2 1.00
269_R 353_E 2.2 1.00
202_L 205_R 2.195 1.00
155_K 354_T 2.125 1.00
68_L 81_S 2.083 1.00
179_I 183_T 2.039 1.00
9_T 13_Q 2.036 1.00
80_E 337_K 2.018 1.00
5_Y 17_G 2.012 1.00
38_L 41_I 1.987 1.00
69_A 147_A 1.968 1.00
272_S 346_V 1.953 1.00
86_G 99_N 1.94 1.00
100_Q 118_F 1.92 1.00
82_L 98_L 1.903 1.00
9_T 15_L 1.874 1.00
387_L 391_L 1.874 1.00
24_E 28_R 1.868 1.00
6_R 16_Q 1.861 1.00
199_Q 205_R 1.86 1.00
203_S 388_Q 1.829 1.00
269_R 349_A 1.826 1.00
126_Y 146_L 1.807 1.00
68_L 143_L 1.8 1.00
261_A 355_L 1.787 1.00
152_N 357_Q 1.763 1.00
172_T 176_G 1.762 1.00
207_L 385_S 1.759 1.00
159_K 361_A 1.758 1.00
126_Y 150_N 1.746 1.00
180_I 184_A 1.737 1.00
285_M 301_L 1.73 1.00
60_E 91_N 1.726 1.00
392_Q 395_S 1.699 1.00
205_R 209_G 1.685 1.00
286_N 305_A 1.679 1.00
73_A 144_E 1.674 1.00
3_Y 24_E 1.665 0.99
23_D 26_Q 1.647 0.99
235_W 241_N 1.608 0.99
115_L 130_V 1.603 0.99
307_N 317_S 1.592 0.99
73_A 347_R 1.59 0.99
112_S 134_E 1.551 0.99
189_I 391_L 1.55 0.99
269_R 350_D 1.538 0.99
30_R 34_E 1.533 0.99
171_T 175_I 1.527 0.99
267_Y 301_L 1.517 0.99
377_L 381_F 1.507 0.99
66_R 151_E 1.498 0.99
65_T 146_L 1.488 0.99
157_R 161_I 1.481 0.99
6_R 40_D 1.473 0.98
176_G 180_I 1.473 0.98
220_T 224_T 1.471 0.98
27_A 38_L 1.46 0.98
246_H 295_L 1.46 0.98
116_Q 131_K 1.459 0.98
104_A 109_H 1.459 0.98
100_Q 104_A 1.453 0.98
6_R 14_K 1.423 0.98
213_T 221_L 1.42 0.98
246_H 294_N 1.415 0.98
201_P 204_T 1.41 0.98
265_A 353_E 1.398 0.98
178_V 378_I 1.389 0.98
281_L 314_I 1.381 0.97
226_F 230_V 1.38 0.97
132_A 136_S 1.38 0.97
259_I 263_N 1.376 0.97
233_W 237_K 1.374 0.97
86_G 102_R 1.374 0.97
102_R 106_L 1.359 0.97
264_S 328_M 1.357 0.97
7_A 17_G 1.356 0.97
168_C 172_T 1.35 0.97
159_K 357_Q 1.337 0.97
170_L 379_V 1.328 0.97
135_K 283_D 1.32 0.96
123_D 126_Y 1.316 0.96
17_G 31_L 1.314 0.96
26_Q 30_R 1.312 0.96
104_A 114_A 1.309 0.96
235_W 238_R 1.296 0.96
219_P 223_A 1.292 0.96
307_N 312_N 1.292 0.96
5_Y 31_L 1.291 0.96
170_L 375_M 1.288 0.96
8_M 14_K 1.28 0.95
243_H 296_E 1.278 0.95
271_L 284_G 1.274 0.95
83_A 102_R 1.266 0.95
276_S 342_G 1.265 0.95
227_I 231_G 1.262 0.95
244_R 247_A 1.258 0.95
203_S 206_I 1.257 0.95
89_S 94_L 1.256 0.95
380_L 384_V 1.253 0.95
162_Q 361_A 1.253 0.95
66_R 147_A 1.246 0.95
188_K 191_E 1.244 0.95
36_L 39_L 1.233 0.94
228_V 232_F 1.231 0.94
68_L 78_L 1.227 0.94
301_L 304_A 1.227 0.94
22_N 26_Q 1.224 0.94
189_I 387_L 1.223 0.94
328_M 348_A 1.221 0.94
73_A 140_A 1.22 0.94
295_L 298_R 1.208 0.93
261_A 359_R 1.194 0.93
182_L 210_L 1.193 0.93
271_L 345_M 1.19 0.93
331_M 348_A 1.166 0.92
148_D 152_N 1.165 0.92
305_A 309_R 1.16 0.91
289_T 305_A 1.151 0.91
200_L 204_T 1.151 0.91
274_L 284_G 1.15 0.91
257_P 261_A 1.142 0.90
104_A 118_F 1.136 0.90
214_L 221_L 1.118 0.89
182_L 186_V 1.107 0.89
244_R 248_M 1.101 0.88
242_R 246_H 1.094 0.88
283_D 286_N 1.093 0.88
70_T 338_S 1.093 0.88
178_V 382_I 1.093 0.88
307_N 320_Q 1.091 0.88
149_Y 153_R 1.088 0.87
165_I 169_M 1.086 0.87
213_T 217_T 1.082 0.87
152_N 353_E 1.079 0.87
78_L 111_L 1.075 0.87
248_M 252_V 1.071 0.86
236_L 245_F 1.068 0.86
174_A 391_L 1.065 0.86
154_Q 158_S 1.064 0.86
158_S 354_T 1.063 0.86
182_L 214_L 1.056 0.85
246_H 249_L 1.056 0.85
250_L 294_N 1.055 0.85
261_A 356_Q 1.051 0.85
56_I 121_L 1.051 0.85
300_R 321_T 1.045 0.84
335_G 339_G 1.045 0.84
312_N 316_L 1.042 0.84
387_L 394_N 1.04 0.84
123_D 150_N 1.04 0.84
368_E 372_I 1.038 0.84
59_S 158_S 1.035 0.84
212_D 215_Q 1.032 0.83
204_T 385_S 1.028 0.83
225_V 229_A 1.025 0.83
75_A 278_G 1.019 0.82
271_L 281_L 1.018 0.82
174_A 382_I 1.016 0.82
242_R 295_L 1.015 0.82
297_I 323_I 1.014 0.82
58_H 157_R 1.013 0.82
389_P 392_Q 1.011 0.82
319_E 322_A 1.009 0.81
62_T 147_A 1.009 0.81
131_K 135_K 1.003 0.81
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3c1qA20.30499.90.73Contact Map0.809
4hhxA10.253899.90.736Contact Map0.736
2whnA20.276499.90.757Contact Map0.875
4ev6A50.497529.20.964Contact Map0.31
2m8rA10.256323.90.966Contact Map0.124
3hd7B20.231223.90.966Contact Map0.266
4i0uA100.492522.80.966Contact Map0.306
3tweA20.065317.10.968Contact Map0.696
4g3bA20.057816.20.968Contact Map0.601
3hd7A20.226110.50.971Contact Map0.096
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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