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OPENSEQ.org
HYBB - Probable Ni/Fe-hydrogenase 2 b-type cytochrome subunit
UniProt: P37180 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11800
Length: 392 (371)
Sequences: 584
Seq/Len: 1.57
HYBB
Paralog alert: 0.28 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: HYBB NRFD
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
30_L 353_L 2.528 1.00
24_I 361_I 2.339 1.00
34_R 54_W 2.316 1.00
20_F 360_A 2.168 0.99
107_T 116_L 2.014 0.98
25_V 28_M 2.009 0.98
319_L 323_L 1.963 0.98
21_G 25_V 1.941 0.98
42_V 350_E 1.861 0.97
307_L 313_M 1.857 0.97
24_I 364_C 1.804 0.96
293_L 297_F 1.796 0.96
29_L 33_K 1.79 0.96
251_T 314_L 1.752 0.95
28_M 32_V 1.719 0.94
33_K 37_F 1.702 0.94
18_M 22_P 1.699 0.94
18_M 28_M 1.697 0.94
44_D 348_T 1.673 0.93
90_P 229_G 1.629 0.92
285_S 289_W 1.622 0.92
26_I 353_L 1.621 0.92
133_E 137_C 1.502 0.87
252_N 256_V 1.495 0.87
84_Y 249_K 1.493 0.87
26_I 30_L 1.491 0.87
305_A 309_N 1.489 0.87
368_V 371_R 1.488 0.86
34_R 39_L 1.479 0.86
175_A 181_P 1.46 0.85
302_L 317_S 1.452 0.85
301_V 307_L 1.439 0.84
205_S 335_V 1.432 0.83
100_S 142_I 1.425 0.83
26_I 356_I 1.412 0.82
22_P 29_L 1.407 0.82
19_I 356_I 1.389 0.81
23_L 360_A 1.386 0.81
23_L 26_I 1.385 0.81
234_A 239_N 1.381 0.80
31_I 108_I 1.379 0.80
22_P 26_I 1.378 0.80
52_G 333_S 1.377 0.80
59_L 141_Y 1.369 0.80
353_L 356_I 1.369 0.80
43_S 54_W 1.359 0.79
97_F 365_A 1.357 0.79
57_F 326_A 1.354 0.78
33_K 350_E 1.34 0.77
345_Y 351_E 1.337 0.77
58_D 106_I 1.334 0.77
45_L 54_W 1.321 0.76
22_P 28_M 1.32 0.76
34_R 42_V 1.314 0.76
223_S 318_A 1.309 0.75
64_G 67_C 1.302 0.75
22_P 356_I 1.3 0.74
25_V 29_L 1.297 0.74
29_L 32_V 1.293 0.74
251_T 302_L 1.269 0.72
220_M 325_C 1.263 0.71
133_E 187_S 1.262 0.71
231_L 235_G 1.254 0.71
352_L 356_I 1.253 0.71
24_I 42_V 1.244 0.70
39_L 54_W 1.241 0.69
95_S 145_M 1.237 0.69
187_S 191_L 1.231 0.69
53_V 352_L 1.23 0.68
262_I 295_M 1.22 0.68
23_L 147_L 1.216 0.67
24_I 28_M 1.215 0.67
103_G 107_T 1.214 0.67
34_R 350_E 1.206 0.66
94_A 228_E 1.204 0.66
19_I 23_L 1.202 0.66
298_P 324_G 1.202 0.66
47_G 125_F 1.192 0.65
117_P 121_I 1.19 0.65
54_W 58_D 1.188 0.65
66_A 98_G 1.186 0.64
185_Q 265_R 1.182 0.64
294_L 364_C 1.181 0.64
101_L 362_E 1.176 0.63
69_G 295_M 1.174 0.63
12_I 15_K 1.173 0.63
87_L 131_L 1.17 0.63
245_S 292_V 1.167 0.63
183_M 268_E 1.163 0.62
181_P 185_Q 1.163 0.62
227_F 322_L 1.162 0.62
27_C 30_L 1.16 0.62
255_S 303_R 1.153 0.61
93_L 369_L 1.151 0.61
131_L 324_G 1.148 0.61
109_D 184_H 1.144 0.60
42_V 353_L 1.144 0.60
42_V 349_W 1.144 0.60
247_F 314_L 1.142 0.60
91_A 141_Y 1.138 0.60
312_R 316_L 1.133 0.59
355_S 361_I 1.132 0.59
34_R 43_S 1.128 0.59
134_T 145_M 1.127 0.59
177_G 189_G 1.124 0.58
179_L 265_R 1.124 0.58
67_C 216_T 1.117 0.57
245_S 249_K 1.115 0.57
100_S 103_G 1.113 0.57
94_A 152_A 1.109 0.57
94_A 98_G 1.106 0.56
69_G 145_M 1.104 0.56
294_L 297_F 1.104 0.56
19_I 32_V 1.104 0.56
21_G 239_N 1.098 0.56
66_A 224_I 1.095 0.55
209_L 318_A 1.094 0.55
85_H 90_P 1.093 0.55
298_P 321_A 1.087 0.54
56_A 334_L 1.086 0.54
103_G 131_L 1.083 0.54
29_L 37_F 1.082 0.54
370_I 373_L 1.082 0.54
170_M 180_L 1.081 0.54
185_Q 188_M 1.081 0.54
63_T 254_I 1.08 0.54
207_E 213_S 1.079 0.54
185_Q 193_I 1.077 0.53
137_C 145_M 1.074 0.53
33_K 42_V 1.072 0.53
227_F 318_A 1.072 0.53
28_M 100_S 1.064 0.52
134_T 188_M 1.06 0.52
15_K 18_M 1.057 0.51
296_L 307_L 1.055 0.51
150_A 250_L 1.054 0.51
53_V 349_W 1.054 0.51
301_V 313_M 1.054 0.51
18_M 37_F 1.052 0.51
129_S 210_P 1.052 0.51
64_G 233_Q 1.051 0.51
58_D 189_G 1.051 0.51
66_A 180_L 1.048 0.50
144_V 176_L 1.043 0.50
259_A 300_V 1.043 0.50
128_N 164_Q 1.042 0.50
136_V 144_V 1.039 0.49
53_V 355_S 1.037 0.49
172_F 308_R 1.037 0.49
147_L 173_I 1.037 0.49
60_L 359_V 1.036 0.49
72_L 174_I 1.036 0.49
23_L 146_A 1.035 0.49
71_A 318_A 1.035 0.49
232_V 366_Y 1.034 0.49
134_T 184_H 1.033 0.49
188_M 191_L 1.032 0.49
180_L 193_I 1.032 0.49
21_G 29_L 1.028 0.48
235_G 348_T 1.027 0.48
25_V 320_S 1.026 0.48
148_E 184_H 1.025 0.48
25_V 32_V 1.025 0.48
32_V 36_V 1.025 0.48
18_M 29_L 1.024 0.48
39_L 45_L 1.023 0.48
64_G 188_M 1.023 0.48
234_A 273_D 1.022 0.48
177_G 181_P 1.021 0.48
144_V 180_L 1.02 0.47
115_N 200_H 1.018 0.47
170_M 265_R 1.017 0.47
28_M 345_Y 1.017 0.47
181_P 268_E 1.017 0.47
147_L 300_V 1.011 0.46
47_G 50_P 1.005 0.46
34_R 52_G 1.004 0.46
178_A 296_L 1.003 0.46
107_T 138_M 1.002 0.46
39_L 43_S 1.002 0.46
275_L 278_A 1.001 0.45
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2vpzC20.63521000.644Contact Map0.196
4i0uA100.173524.40.968Contact Map0.421
4ev6A50.173512.70.972Contact Map0.302
2qi9A20.65059.90.973Contact Map0.224
4g1uA20.65827.80.975Contact Map0.259
3mk7A40.59697.80.975Contact Map0.152
3tdsE50.23476.50.975Contact Map0.25
3l1lA10.87765.40.976Contact Map0.076
2kncA10.12244.80.977Contact Map0
2nq2A20.66584.60.977Contact Map0.239
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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