May 4, 2021 - We are working on upgrading the webserver, some pages may not work.
OPENSEQ.org
NRFD - Protein NrfD
UniProt: P32709 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11947
Length: 318 (309)
Sequences: 915
Seq/Len: 2.96
NRFD
Paralog alert: 0.70 [within 20: 0.02] - ratio of genomes with paralogs
Cluster includes: HYBB NRFD
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
29_A 156_A 2.49 1.00
97_G 162_Y 2.321 1.00
32_V 152_M 2.245 1.00
28_S 104_Y 2.219 1.00
72_V 81_F 2.162 1.00
260_T 264_W 2.086 1.00
21_Y 71_L 2.043 1.00
74_H 163_T 2.037 1.00
195_A 295_C 1.91 0.99
33_T 156_A 1.908 0.99
296_G 300_V 1.863 0.99
275_P 301_G 1.681 0.98
271_G 275_P 1.668 0.98
160_G 189_G 1.638 0.98
257_G 264_W 1.634 0.98
219_R 277_L 1.618 0.97
72_V 78_P 1.615 0.97
107_V 155_L 1.584 0.97
55_T 201_M 1.578 0.97
68_L 72_V 1.574 0.97
20_I 303_L 1.563 0.97
21_Y 97_G 1.482 0.95
133_I 136_K 1.468 0.95
188_S 306_R 1.466 0.95
97_G 101_F 1.466 0.95
214_A 226_W 1.438 0.94
179_P 253_A 1.411 0.93
26_G 228_E 1.41 0.93
199_I 295_C 1.409 0.93
21_Y 162_Y 1.4 0.93
97_G 163_T 1.4 0.93
192_S 302_V 1.393 0.93
114_K 117_E 1.383 0.92
22_L 26_G 1.381 0.92
129_P 133_I 1.371 0.92
289_P 293_A 1.367 0.92
19_A 23_F 1.363 0.92
28_S 159_L 1.334 0.90
191_S 298_S 1.332 0.90
76_T 161_A 1.33 0.90
35_A 56_T 1.31 0.89
188_S 228_E 1.304 0.89
136_K 148_L 1.302 0.89
148_L 199_I 1.287 0.88
199_I 299_L 1.279 0.88
257_G 263_F 1.262 0.87
206_R 293_A 1.257 0.87
96_M 100_L 1.25 0.86
82_W 86_F 1.247 0.86
97_G 305_L 1.246 0.86
94_M 193_G 1.243 0.86
228_E 305_L 1.243 0.86
104_Y 159_L 1.24 0.86
68_L 102_Q 1.236 0.85
229_I 233_V 1.233 0.85
28_S 63_A 1.22 0.84
256_G 260_T 1.219 0.84
284_R 290_A 1.218 0.84
229_I 278_L 1.217 0.84
131_L 137_V 1.216 0.84
282_V 286_S 1.216 0.84
218_H 291_V 1.214 0.84
185_F 309_I 1.21 0.84
159_L 163_T 1.207 0.83
264_W 277_L 1.2 0.83
67_G 191_S 1.197 0.83
188_S 305_L 1.194 0.82
147_G 151_L 1.188 0.82
71_L 97_G 1.184 0.82
25_I 165_F 1.182 0.82
17_P 73_F 1.18 0.81
88_Y 95_S 1.179 0.81
261_W 265_L 1.179 0.81
191_S 225_V 1.176 0.81
72_V 101_F 1.172 0.81
34_L 217_V 1.171 0.81
65_I 68_L 1.17 0.81
92_S 182_P 1.17 0.81
250_L 266_G 1.169 0.81
133_I 137_V 1.167 0.80
173_Y 182_P 1.165 0.80
197_A 217_V 1.16 0.80
135_Q 139_S 1.159 0.80
19_A 311_Y 1.158 0.80
166_L 188_S 1.154 0.79
19_A 310_L 1.153 0.79
132_G 135_Q 1.151 0.79
236_F 240_A 1.15 0.79
18_I 104_Y 1.146 0.79
199_I 292_L 1.144 0.79
56_T 104_Y 1.142 0.78
220_M 278_L 1.14 0.78
274_V 297_A 1.14 0.78
231_L 235_F 1.135 0.78
31_L 197_A 1.131 0.77
184_L 188_S 1.127 0.77
74_H 101_F 1.127 0.77
12_L 311_Y 1.117 0.76
219_R 227_G 1.116 0.76
184_L 306_R 1.115 0.76
143_P 280_P 1.105 0.75
184_L 271_G 1.104 0.75
226_W 229_I 1.103 0.75
278_L 294_A 1.103 0.75
72_V 75_L 1.102 0.75
10_E 76_T 1.101 0.75
21_Y 164_G 1.099 0.75
71_L 75_L 1.099 0.75
130_K 134_V 1.099 0.75
153_L 186_L 1.098 0.74
41_F 49_D 1.098 0.74
100_L 159_L 1.097 0.74
218_H 221_E 1.092 0.74
295_C 299_L 1.092 0.74
60_G 213_E 1.092 0.74
265_L 290_A 1.091 0.74
278_L 293_A 1.091 0.74
202_A 295_C 1.085 0.73
107_V 151_L 1.085 0.73
219_R 223_P 1.085 0.73
277_L 290_A 1.081 0.73
178_N 312_A 1.079 0.73
69_L 227_G 1.077 0.72
118_H 121_L 1.077 0.72
273_I 296_G 1.076 0.72
106_V 110_L 1.068 0.72
278_L 284_R 1.066 0.71
131_L 134_V 1.061 0.71
26_G 97_G 1.058 0.71
15_D 76_T 1.057 0.70
126_R 137_V 1.056 0.70
233_V 293_A 1.056 0.70
22_L 166_L 1.053 0.70
65_I 220_M 1.051 0.70
61_P 109_V 1.05 0.70
35_A 185_F 1.05 0.70
67_G 184_L 1.048 0.69
242_G 249_A 1.045 0.69
311_Y 314_Q 1.043 0.69
221_E 224_V 1.04 0.69
225_V 272_L 1.038 0.68
165_F 182_P 1.037 0.68
231_L 234_A 1.035 0.68
137_V 274_V 1.035 0.68
65_I 105_M 1.029 0.67
59_V 269_G 1.026 0.67
32_V 57_L 1.026 0.67
108_L 111_W 1.024 0.67
274_V 282_V 1.02 0.66
64_V 68_L 1.02 0.66
19_A 307_F 1.018 0.66
71_L 162_Y 1.017 0.66
23_F 37_L 1.017 0.66
160_G 186_L 1.017 0.66
265_L 292_L 1.016 0.66
180_I 185_F 1.015 0.66
22_L 97_G 1.014 0.66
78_P 81_F 1.014 0.66
59_V 299_L 1.011 0.65
20_I 89_S 1.011 0.65
179_P 183_V 1.009 0.65
143_P 296_G 1.008 0.65
271_G 297_A 1.003 0.65
128_L 137_V 1.003 0.65
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2vpzC20.7831000.391Contact Map0.311
3rkoA20.424518.20.958Contact Map0.314
4c7rA30.927720.973Contact Map0.178
4he8A20.34911.80.974Contact Map0.226
2k1eA40.16041.70.975Contact Map0.238
2kncA10.14151.70.975Contact Map0.374
4ainA30.92771.60.975Contact Map0.186
1jb0I10.10381.60.975Contact Map0.239
2a9hA40.17611.50.975Contact Map0.31
3dh4A40.63211.30.976Contact Map0.144
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
Page generated in 0.0813 seconds.