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OPENSEQ.org
RIHB - Pyrimidine-specific ribonucleoside hydrolase RihB
UniProt: P33022 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12030
Length: 313 (308)
Sequences: 1830
Seq/Len: 5.94
RIHB
Paralog alert: 0.64 [within 20: 0.03] - ratio of genomes with paralogs
Cluster includes: RIHA RIHB RIHC
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
102_T 110_D 3.954 1.00
106_K 136_M 3.646 1.00
5_K 30_D 2.901 1.00
167_I 273_T 2.807 1.00
135_R 176_V 2.634 1.00
122_V 244_I 2.616 1.00
33_G 107_Y 2.595 1.00
23_A 244_I 2.551 1.00
175_R 179_T 2.532 1.00
194_Q 292_T 2.494 1.00
175_R 261_E 2.35 1.00
134_M 141_L 2.333 1.00
109_I 133_A 2.318 1.00
123_P 149_L 2.301 1.00
249_N 297_W 2.277 1.00
36_I 47_L 2.27 1.00
107_Y 111_T 2.241 1.00
4_R 146_E 2.236 1.00
67_M 132_V 2.232 1.00
254_K 292_T 2.228 1.00
8_L 124_V 2.215 1.00
192_T 239_H 2.188 1.00
12_P 50_G 2.16 1.00
133_A 137_Q 2.104 1.00
109_I 137_Q 2.085 1.00
110_D 114_A 2.065 1.00
9_D 129_N 2.052 1.00
20_I 34_I 2.032 1.00
32_L 119_I 2.031 1.00
24_A 31_L 1.947 1.00
113_M 137_Q 1.946 1.00
26_H 305_C 1.923 1.00
263_D 269_C 1.923 1.00
123_P 130_I 1.915 1.00
112_L 119_I 1.876 1.00
35_T 104_A 1.871 1.00
109_I 140_I 1.869 1.00
7_I 35_T 1.865 1.00
113_M 139_A 1.845 1.00
5_K 32_L 1.829 1.00
167_I 174_A 1.823 1.00
10_C 124_V 1.81 1.00
21_M 57_L 1.781 1.00
113_M 143_K 1.766 1.00
51_L 63_V 1.759 1.00
238_V 241_A 1.746 1.00
120_T 148_V 1.731 1.00
51_L 98_Q 1.72 1.00
204_R 300_G 1.717 1.00
127_L 149_L 1.715 1.00
54_C 61_V 1.69 1.00
127_L 177_V 1.689 1.00
252_G 297_W 1.663 1.00
64_Y 107_Y 1.661 1.00
6_I 122_V 1.649 1.00
255_T 290_G 1.641 1.00
296_D 300_G 1.624 1.00
118_D 145_R 1.624 1.00
172_E 270_Y 1.613 0.99
137_Q 140_I 1.587 0.99
55_Q 95_L 1.573 0.99
73_R 270_Y 1.573 0.99
162_S 272_R 1.571 0.99
112_L 133_A 1.564 0.99
64_Y 100_E 1.562 0.99
8_L 31_L 1.545 0.99
206_E 217_S 1.544 0.99
246_Y 253_I 1.542 0.99
294_D 297_W 1.491 0.99
146_E 185_V 1.49 0.99
43_L 65_A 1.476 0.99
149_L 178_F 1.469 0.99
123_P 127_L 1.446 0.99
133_A 140_I 1.445 0.99
106_K 110_D 1.424 0.98
257_E 288_K 1.408 0.98
108_I 133_A 1.396 0.98
115_S 118_D 1.395 0.98
130_I 147_I 1.39 0.98
167_I 178_F 1.39 0.98
149_L 167_I 1.365 0.98
151_G 167_I 1.352 0.98
4_R 145_R 1.351 0.98
120_T 146_E 1.351 0.98
266_S 269_C 1.338 0.97
28_A 305_C 1.335 0.97
108_I 130_I 1.328 0.97
123_P 147_I 1.328 0.97
10_C 50_G 1.309 0.97
245_G 298_F 1.301 0.97
242_T 293_I 1.288 0.97
56_K 215_L 1.268 0.96
111_T 114_A 1.247 0.96
105_V 133_A 1.239 0.95
149_L 186_M 1.236 0.95
187_M 290_G 1.227 0.95
178_F 186_M 1.227 0.95
13_G 17_A 1.227 0.95
48_I 52_N 1.226 0.95
36_I 50_G 1.209 0.95
214_E 218_D 1.19 0.94
269_C 272_R 1.189 0.94
263_D 268_P 1.188 0.94
35_T 129_N 1.186 0.94
160_T 163_A 1.179 0.94
112_L 143_K 1.178 0.93
7_I 33_G 1.176 0.93
23_A 122_V 1.176 0.93
43_L 47_L 1.176 0.93
269_C 274_V 1.175 0.93
83_G 86_G 1.168 0.93
108_I 140_I 1.166 0.93
261_E 268_P 1.161 0.93
134_M 144_I 1.157 0.93
55_Q 97_R 1.152 0.92
116_D 143_K 1.149 0.92
103_H 106_K 1.14 0.92
242_T 253_I 1.136 0.92
31_L 124_V 1.132 0.92
7_I 107_Y 1.13 0.92
201_V 299_W 1.124 0.91
105_V 109_I 1.117 0.91
22_M 301_L 1.102 0.90
189_L 239_H 1.095 0.90
203_A 207_R 1.092 0.90
185_V 246_Y 1.089 0.89
158_N 163_A 1.081 0.89
258_M 275_C 1.079 0.89
262_V 273_T 1.066 0.88
142_P 145_R 1.065 0.88
260_V 287_T 1.061 0.88
112_L 140_I 1.058 0.88
106_K 109_I 1.056 0.87
54_C 63_V 1.054 0.87
48_I 94_P 1.052 0.87
82_H 86_G 1.047 0.87
199_P 203_A 1.045 0.87
251_D 297_W 1.044 0.87
8_L 23_A 1.042 0.86
144_I 182_V 1.039 0.86
25_K 309_Y 1.033 0.86
54_C 60_N 1.032 0.86
175_R 264_V 1.027 0.85
147_I 182_V 1.027 0.85
103_H 136_M 1.026 0.85
121_L 144_I 1.025 0.85
11_D 15_D 1.025 0.85
157_G 163_A 1.023 0.85
64_Y 102_T 1.021 0.85
263_D 266_S 1.019 0.85
4_R 120_T 1.018 0.85
111_T 119_I 1.015 0.84
24_A 54_C 1.012 0.84
67_M 103_H 1.009 0.84
72_M 172_E 1.005 0.84
134_M 180_S 1.004 0.84
51_L 99_A 1.004 0.84
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1q8fA411000.019Contact Map0.837
1yoeA10.99041000.033Contact Map0.811
2masA40.99361000.036Contact Map0.819
4kpoA20.9841000.046Contact Map0.783
4kpnA80.9841000.048Contact Map0.814
3t8jA10.97761000.054Contact Map0.735
3t8iA40.96811000.07Contact Map0.816
3fz0A40.98721000.071Contact Map0.76
4i71A10.96811000.119Contact Map0.722
3epwA20.96811000.129Contact Map0.723
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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