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PFLC - Pyruvate formate-lyase 2-activating enzyme
UniProt: P32675 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11911
Length: 292 (271)
Sequences: 1302
Seq/Len: 4.80
PFLC
Paralog alert: 0.50 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: NRDG PFLA PFLC PFLE YJJW
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
123_L 135_A 3.67 1.00
121_V 142_L 3.537 1.00
181_A 191_N 3.402 1.00
108_M 145_W 3.145 1.00
100_L 141_R 2.926 1.00
181_A 193_P 2.909 1.00
141_R 144_L 2.795 1.00
101_D 141_R 2.697 1.00
184_A 192_L 2.666 1.00
24_I 44_F 2.501 1.00
148_S 171_E 2.5 1.00
140_Q 168_L 2.429 1.00
174_F 199_L 2.406 1.00
48_P 154_A 2.392 1.00
223_R 276_T 2.355 1.00
183_Q 186_D 2.148 1.00
143_R 170_D 2.124 1.00
155_G 174_F 2.011 1.00
140_Q 164_P 2.005 1.00
107_V 142_L 1.999 1.00
218_G 266_V 1.944 1.00
139_L 149_C 1.932 1.00
260_T 264_K 1.922 1.00
25_F 59_S 1.922 1.00
269_P 274_V 1.888 1.00
213_L 241_I 1.876 1.00
141_R 145_W 1.86 1.00
231_D 284_A 1.823 1.00
40_T 106_E 1.812 1.00
52_P 262_S 1.81 1.00
106_E 109_K 1.786 1.00
104_E 142_L 1.771 1.00
180_D 228_Q 1.764 1.00
123_L 138_F 1.745 1.00
278_R 282_E 1.73 1.00
183_Q 225_N 1.716 1.00
255_R 264_K 1.711 0.99
273_D 276_T 1.71 0.99
208_N 239_R 1.681 0.99
211_P 233_L 1.678 0.99
223_R 227_Q 1.653 0.99
53_W 216_I 1.651 0.99
201_L 205_E 1.649 0.99
55_A 152_E 1.602 0.99
163_L 201_L 1.597 0.99
199_L 232_V 1.571 0.99
270_S 273_D 1.545 0.99
151_I 172_V 1.507 0.98
215_L 243_L 1.489 0.98
222_S 225_N 1.486 0.98
223_R 273_D 1.477 0.98
123_L 139_L 1.477 0.98
128_V 135_A 1.475 0.98
23_R 97_D 1.466 0.98
136_T 164_P 1.465 0.98
28_Q 31_S 1.451 0.98
193_P 197_E 1.447 0.98
179_M 232_V 1.437 0.98
98_I 103_L 1.414 0.97
230_L 281_A 1.402 0.97
22_A 134_F 1.384 0.97
227_Q 283_R 1.382 0.97
22_A 131_Q 1.374 0.97
101_D 105_R 1.369 0.97
178_I 225_N 1.368 0.97
184_A 195_V 1.354 0.96
275_A 278_R 1.353 0.96
228_Q 231_D 1.347 0.96
185_R 191_N 1.343 0.96
99_S 102_A 1.331 0.96
209_V 237_N 1.32 0.96
128_V 151_I 1.319 0.96
96_R 106_E 1.318 0.96
221_L 273_D 1.315 0.95
224_E 228_Q 1.314 0.95
159_A 201_L 1.313 0.95
215_L 274_V 1.284 0.95
52_P 186_D 1.278 0.94
98_I 102_A 1.278 0.94
184_A 246_F 1.242 0.93
100_L 138_F 1.209 0.92
233_L 241_I 1.205 0.92
21_V 97_D 1.203 0.92
48_P 130_M 1.2 0.92
196_L 232_V 1.186 0.91
60_I 259_K 1.183 0.91
163_L 205_E 1.167 0.90
104_E 141_R 1.158 0.90
227_Q 280_M 1.151 0.89
152_E 210_I 1.148 0.89
275_A 279_E 1.144 0.89
227_Q 284_A 1.14 0.89
200_R 232_V 1.139 0.89
129_L 165_L 1.137 0.89
139_L 169_C 1.129 0.88
179_M 228_Q 1.127 0.88
192_L 195_V 1.119 0.87
172_V 199_L 1.11 0.87
271_S 275_A 1.109 0.87
187_V 219_F 1.107 0.87
35_G 39_R 1.105 0.87
169_C 172_V 1.105 0.87
108_M 111_D 1.102 0.86
79_C 86_C 1.101 0.86
279_E 283_R 1.097 0.86
49_H 195_V 1.095 0.86
241_I 281_A 1.088 0.85
103_L 138_F 1.083 0.85
73_C 76_C 1.08 0.85
226_M 277_M 1.076 0.85
179_M 225_N 1.073 0.84
172_V 207_V 1.059 0.83
104_E 108_M 1.048 0.82
111_D 115_R 1.044 0.82
182_T 185_R 1.043 0.82
178_I 188_V 1.038 0.82
24_I 98_I 1.036 0.81
201_L 204_S 1.033 0.81
143_R 168_L 1.032 0.81
155_G 198_N 1.032 0.81
107_V 121_V 1.028 0.81
42_V 107_V 1.025 0.80
200_R 204_S 1.02 0.80
251_E 265_E 1.018 0.80
167_K 205_E 1.015 0.79
49_H 190_M 1.015 0.79
133_E 161_K 1.013 0.79
213_L 229_A 1.012 0.79
178_I 183_Q 1.009 0.79
155_G 199_L 1.007 0.79
152_E 173_L 1.007 0.79
210_I 240_Q 1 0.78
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3c8fA10.82881000.547Contact Map0.75
3canA10.60961000.652Contact Map0.757
3rfaA20.82531000.705Contact Map0.61
2yx0A10.86991000.725Contact Map0.676
2z2uA10.85621000.73Contact Map0.663
1hfeL20.832299.90.746Contact Map0.112
1oltA10.869999.90.761Contact Map0.457
1tv8A20.767199.90.766Contact Map0.768
2qgqA80.7599.90.773Contact Map0.687
1r30A20.845999.80.789Contact Map0.53
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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