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OPENSEQ.org

PFLA - Pyruvate formate-lyase 1-activating enzyme
UniProt: P0A9N4 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10028
Length: 246 (241)
Sequences: 2491
Seq/Len: 10.34

PFLA
Paralog alert: 0.54 [within 20: 0.02] - ratio of genomes with paralogs
Cluster includes: NRDG PFLA PFLC PFLE YJJW
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
61_V 98_E 3.462 1.00
74_V 95_C 2.819 1.00
234_K 238_E 2.734 1.00
136_D 146_S 2.577 1.00
94_A 97_K 2.521 1.00
168_Y 197_I 2.477 1.00
186_E 240_Y 2.444 1.00
178_D 232_R 2.439 1.00
54_E 94_A 2.432 1.00
93_R 123_V 2.409 1.00
76_A 88_V 2.409 1.00
31_L 107_N 2.324 1.00
76_A 102_T 2.308 1.00
166_I 188_T 2.304 1.00
136_D 148_H 2.264 1.00
92_F 102_T 2.236 1.00
96_K 125_D 2.223 1.00
118_D 156_Y 2.171 1.00
89_R 119_E 2.16 1.00
156_Y 159_N 1.972 1.00
139_H 147_N 1.965 1.00
129_L 154_A 1.905 1.00
148_H 152_E 1.883 1.00
182_H 239_Q 1.87 1.00
235_G 239_Q 1.846 1.00
53_V 94_A 1.825 1.00
182_H 240_Y 1.806 1.00
181_A 233_V 1.792 1.00
229_T 232_R 1.773 1.00
101_H 126_L 1.742 1.00
183_R 186_E 1.74 1.00
131_L 154_A 1.718 1.00
177_D 180_S 1.706 1.00
182_H 236_I 1.701 1.00
127_V 157_L 1.683 1.00
108_G 129_L 1.68 1.00
226_K 229_T 1.669 1.00
94_A 98_E 1.663 1.00
138_I 141_N 1.652 1.00
138_I 180_S 1.638 1.00
156_Y 160_K 1.602 1.00
8_H 42_T 1.6 1.00
178_D 182_H 1.561 1.00
152_E 155_K 1.555 1.00
76_A 91_W 1.534 1.00
140_Q 146_S 1.521 1.00
7_I 27_F 1.495 1.00
173_G 222_V 1.485 1.00
127_V 162_V 1.447 1.00
188_T 197_I 1.431 1.00
76_A 92_F 1.431 1.00
60_V 95_C 1.43 1.00
185_G 236_I 1.427 1.00
52_T 55_D 1.398 0.99
11_E 14_G 1.388 0.99
142_L 174_W 1.377 0.99
155_K 159_N 1.37 0.99
27_F 76_A 1.359 0.99
51_V 56_L 1.344 0.99
106_T 129_L 1.341 0.99
158_A 192_G 1.336 0.99
54_E 58_K 1.332 0.99
88_V 104_L 1.331 0.99
178_D 229_T 1.321 0.99
168_Y 184_L 1.319 0.99
163_K 195_E 1.309 0.99
128_M 167_R 1.304 0.99
170_V 233_V 1.296 0.99
59_E 62_T 1.261 0.99
57_M 94_A 1.256 0.99
81_A 88_V 1.25 0.99
38_H 105_D 1.249 0.99
185_G 240_Y 1.249 0.99
154_A 166_I 1.244 0.98
112_R 152_E 1.242 0.98
25_T 60_V 1.229 0.98
92_F 124_T 1.225 0.98
32_M 107_N 1.198 0.98
135_N 180_S 1.197 0.98
134_M 187_F 1.196 0.98
211_V 220_D 1.195 0.98
185_G 237_L 1.19 0.98
225_P 230_M 1.188 0.98
22_R 75_T 1.184 0.98
194_V 243_K 1.181 0.98
174_W 222_V 1.18 0.98
32_M 109_F 1.174 0.98
89_R 93_R 1.167 0.97
135_N 183_R 1.165 0.97
111_R 116_V 1.157 0.97
151_L 187_F 1.155 0.97
96_K 123_V 1.154 0.97
51_V 55_D 1.149 0.97
227_K 231_E 1.147 0.97
81_A 104_L 1.145 0.97
6_R 50_E 1.138 0.97
157_L 164_V 1.136 0.97
52_T 87_F 1.129 0.97
133_Q 180_S 1.121 0.97
35_L 141_N 1.121 0.97
93_R 119_E 1.114 0.96
93_R 97_K 1.107 0.96
36_Y 171_V 1.106 0.96
179_D 183_R 1.101 0.96
155_K 187_F 1.101 0.96
118_D 160_K 1.098 0.96
23_F 59_E 1.097 0.96
31_L 83_L 1.092 0.96
104_L 127_V 1.087 0.96
133_Q 143_V 1.085 0.96
108_G 154_A 1.079 0.95
231_E 235_G 1.076 0.95
89_R 123_V 1.062 0.95
176_D 229_T 1.055 0.95
216_E 220_D 1.047 0.94
102_T 124_T 1.037 0.94
231_E 234_K 1.037 0.94
95_C 100_I 1.036 0.94
147_N 151_L 1.029 0.94
199_L 237_L 1.027 0.94
154_A 187_F 1.023 0.93
57_M 95_C 1.02 0.93
53_V 91_W 1.019 0.93
82_I 110_V 1.017 0.93
117_I 153_F 1.013 0.93
5_G 87_F 1.011 0.93
55_D 62_T 1.011 0.93
233_V 236_I 1.01 0.93
96_K 102_T 1.007 0.93
112_R 156_Y 1.004 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3c8fA10.98781000.394Contact Map0.741
3canA10.71951000.572Contact Map0.775
3rfaA20.91061000.609Contact Map0.624
2yx0A10.94311000.621Contact Map0.708
2z2uA10.92681000.622Contact Map0.688
1tv8A20.882199.90.659Contact Map0.776
1oltA10.967599.90.675Contact Map0.504
2qgqA80.865999.90.694Contact Map0.724
1r30A20.922899.80.702Contact Map0.546
3t7vA10.922899.80.709Contact Map0.604

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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