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OPENSEQ.org
FIXB - Protein FixB
UniProt: P31574 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11563
Length: 313 (308)
Sequences: 1864
Seq/Len: 6.05
FIXB
Paralog alert: 0.42 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: FIXB YDIR YGCQ
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
298_G 303_I 5.48 1.00
289_F 295_G 3.779 1.00
259_V 280_I 3.252 1.00
200_S 261_I 2.964 1.00
24_A 31_I 2.933 1.00
22_N 155_A 2.888 1.00
196_R 254_E 2.768 1.00
198_V 251_L 2.547 1.00
201_V 211_I 2.512 1.00
86_L 96_A 2.467 1.00
32_N 53_W 2.437 1.00
51_H 166_H 2.34 1.00
23_G 26_A 2.269 1.00
45_I 163_G 2.247 1.00
259_V 304_L 2.191 1.00
20_L 85_L 2.179 1.00
228_S 261_I 2.134 1.00
220_A 308_T 2.088 1.00
255_L 278_F 2.057 1.00
217_L 304_L 2.035 1.00
212_A 215_E 1.995 1.00
75_I 84_V 1.977 1.00
279_A 292_A 1.955 1.00
217_L 308_T 1.921 1.00
251_L 271_A 1.906 1.00
212_A 216_Q 1.902 1.00
227_C 238_M 1.872 1.00
25_Q 50_N 1.848 1.00
238_M 242_R 1.844 1.00
216_Q 308_T 1.83 1.00
38_D 56_N 1.826 1.00
214_A 259_V 1.813 1.00
201_V 214_A 1.804 1.00
215_E 225_L 1.801 1.00
197_L 223_A 1.749 1.00
271_A 274_S 1.743 1.00
112_S 133_I 1.743 1.00
200_S 244_V 1.737 1.00
118_D 137_R 1.732 1.00
11_S 33_T 1.702 1.00
158_D 161_R 1.702 1.00
199_V 217_L 1.693 1.00
256_Y 267_H 1.689 1.00
211_I 225_L 1.673 1.00
124_K 133_I 1.669 1.00
268_M 291_Y 1.66 1.00
111_A 145_L 1.655 1.00
38_D 58_K 1.614 1.00
30_Q 51_H 1.609 0.99
259_V 300_A 1.605 0.99
216_Q 312_A 1.593 0.99
96_A 146_T 1.588 0.99
32_N 50_N 1.585 0.99
198_V 226_A 1.585 0.99
113_T 124_K 1.573 0.99
276_T 294_Y 1.557 0.99
72_A 102_R 1.546 0.99
30_Q 50_N 1.545 0.99
200_S 258_A 1.532 0.99
279_A 288_I 1.531 0.99
77_Q 168_V 1.521 0.99
210_N 301_V 1.493 0.99
34_F 71_M 1.491 0.99
46_Q 159_A 1.481 0.99
112_S 124_K 1.478 0.99
68_A 102_R 1.471 0.99
73_D 76_R 1.469 0.99
64_I 94_L 1.458 0.99
306_A 309_A 1.451 0.99
205_I 214_A 1.436 0.99
216_Q 220_A 1.423 0.98
258_A 261_I 1.412 0.98
220_A 312_A 1.407 0.98
227_C 231_V 1.391 0.98
204_G 237_W 1.387 0.98
226_A 244_V 1.386 0.98
111_A 147_I 1.383 0.98
217_L 221_I 1.382 0.98
192_L 198_V 1.381 0.98
67_Y 95_L 1.38 0.98
302_K 306_A 1.372 0.98
213_L 304_L 1.355 0.98
111_A 114_V 1.346 0.98
51_H 164_E 1.337 0.97
208_K 238_M 1.316 0.97
19_E 62_R 1.304 0.97
191_D 194_K 1.296 0.97
205_I 210_N 1.295 0.97
14_P 18_P 1.292 0.97
258_A 267_H 1.281 0.96
204_G 213_L 1.281 0.96
75_I 103_L 1.247 0.96
304_L 308_T 1.238 0.96
210_N 213_L 1.238 0.96
200_S 228_S 1.236 0.95
99_L 144_V 1.236 0.95
110_D 148_S 1.234 0.95
19_E 142_Y 1.222 0.95
38_D 60_D 1.208 0.95
71_M 99_L 1.187 0.94
215_E 219_K 1.176 0.94
45_I 158_D 1.168 0.93
296_I 303_I 1.165 0.93
254_E 275_Q 1.161 0.93
204_G 210_N 1.16 0.93
85_L 145_L 1.156 0.93
6_Q 30_Q 1.148 0.93
72_A 99_L 1.146 0.92
211_I 231_V 1.145 0.92
20_L 87_P 1.138 0.92
108_S 111_A 1.135 0.92
239_E 242_R 1.133 0.92
205_I 300_A 1.131 0.92
8_W 74_T 1.126 0.92
73_D 77_Q 1.12 0.91
207_S 237_W 1.119 0.91
306_A 310_A 1.114 0.91
19_E 28_A 1.11 0.91
19_E 150_G 1.104 0.90
219_K 222_G 1.103 0.90
213_L 216_Q 1.101 0.90
69_G 73_D 1.093 0.90
45_I 161_R 1.093 0.90
53_W 168_V 1.089 0.90
43_Q 157_P 1.088 0.90
122_T 135_E 1.087 0.90
78_H 166_H 1.084 0.89
289_F 297_V 1.083 0.89
55_L 70_V 1.082 0.89
49_A 52_V 1.082 0.89
192_L 226_A 1.074 0.89
213_L 308_T 1.072 0.89
11_S 44_A 1.066 0.88
7_V 24_A 1.064 0.88
197_L 257_L 1.06 0.88
21_M 49_A 1.053 0.88
28_A 63_M 1.051 0.87
25_Q 31_I 1.049 0.87
244_V 271_A 1.048 0.87
115_S 124_K 1.047 0.87
205_I 237_W 1.044 0.87
227_C 232_A 1.041 0.87
36_L 70_V 1.04 0.87
34_F 74_T 1.034 0.86
53_W 74_T 1.031 0.86
82_G 142_Y 1.028 0.86
199_V 304_L 1.024 0.85
300_A 305_P 1.019 0.85
21_M 47_L 1.015 0.85
27_L 85_L 1.011 0.84
127_V 132_A 1.004 0.84
179_R 182_T 1.003 0.84
206_G 301_V 1.003 0.84
42_A 54_K 1.002 0.84
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1efvA10.97121000.059Contact Map0.807
1o97D10.96491000.061Contact Map0.679
1efpA20.94571000.077Contact Map0.774
3ih5A40.60061000.658Contact Map0.804
3fetA40.53041000.72Contact Map0.732
1efvB10.65599.90.76Contact Map0.694
1efpB20.658199.90.765Contact Map0.693
1o97C10.674199.90.767Contact Map0.7
1ozhA40.920195.30.936Contact Map0.649
2pgnA20.9297950.937Contact Map0.611
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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