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OPENSEQ.org
RFAQ - Lipopolysaccharide core heptosyltransferase RfaQ
UniProt: P25742 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11341
Length: 344 (332)
Sequences: 2523
Seq/Len: 7.60
RFAQ
Paralog alert: 0.82 [within 20: 0.45] - ratio of genomes with paralogs
Cluster includes: RFAC RFAF RFAQ WAAU
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
27_D 76_Y 4.564 1.00
175_V 251_A 4.002 1.00
253_L 327_T 3.606 1.00
198_A 324_I 2.909 1.00
46_A 74_N 2.829 1.00
191_K 320_A 2.815 1.00
176_I 196_I 2.694 1.00
192_F 257_V 2.661 1.00
308_L 314_Y 2.643 1.00
262_G 274_S 2.631 1.00
244_L 261_P 2.606 1.00
175_V 248_I 2.587 1.00
18_K 45_N 2.483 1.00
189_N 222_E 2.399 1.00
240_R 243_E 2.311 1.00
301_K 308_L 2.273 1.00
187_W 323_V 2.219 1.00
195_V 324_I 2.208 1.00
193_S 226_G 2.171 1.00
301_K 314_Y 2.155 1.00
304_T 308_L 2.139 1.00
256_G 262_G 2.13 1.00
292_I 329_K 2.116 1.00
25_K 46_A 2.098 1.00
7_M 38_L 2.091 1.00
27_D 45_N 2.076 1.00
304_T 314_Y 2.071 1.00
175_V 209_T 2.05 1.00
195_V 255_I 2.048 1.00
188_D 191_K 2.035 1.00
25_K 45_N 2.022 1.00
156_C 242_P 2.009 1.00
173_Y 250_H 2.005 1.00
210_C 237_G 1.997 1.00
301_K 304_T 1.976 1.00
173_Y 251_A 1.944 1.00
27_D 46_A 1.915 1.00
255_I 327_T 1.91 1.00
13_V 82_L 1.891 1.00
174_V 199_L 1.846 1.00
202_R 328_E 1.829 1.00
159_K 243_E 1.815 1.00
157_W 249_D 1.776 1.00
172_H 252_V 1.761 1.00
198_A 321_E 1.72 1.00
136_L 141_I 1.719 1.00
208_L 220_V 1.701 1.00
187_W 191_K 1.677 1.00
217_L 221_D 1.629 1.00
173_Y 252_V 1.626 1.00
161_R 250_H 1.602 1.00
174_V 253_L 1.584 1.00
197_D 228_E 1.569 1.00
193_S 197_D 1.557 1.00
220_V 236_A 1.539 1.00
36_P 39_S 1.534 1.00
197_D 227_C 1.53 1.00
15_S 149_T 1.521 1.00
27_D 48_Y 1.515 1.00
321_E 325_A 1.505 0.99
18_K 26_I 1.496 0.99
245_G 264_I 1.482 0.99
194_K 197_D 1.478 0.99
234_G 237_G 1.477 0.99
198_A 202_R 1.46 0.99
191_K 194_K 1.432 0.99
7_M 28_M 1.428 0.99
185_K 258_D 1.419 0.99
292_I 326_A 1.413 0.99
10_T 82_L 1.398 0.99
65_L 69_K 1.388 0.99
68_I 72_R 1.382 0.99
209_T 244_L 1.379 0.99
173_Y 207_V 1.374 0.99
156_C 243_E 1.372 0.99
35_I 39_S 1.372 0.99
27_D 74_N 1.367 0.99
313_K 317_V 1.365 0.99
187_W 192_F 1.347 0.99
171_D 205_Q 1.345 0.99
9_L 284_W 1.343 0.99
13_V 136_L 1.332 0.98
210_C 234_G 1.329 0.98
262_G 276_F 1.329 0.98
254_F 270_T 1.325 0.98
253_L 273_I 1.322 0.98
18_K 24_A 1.319 0.98
174_V 204_Y 1.316 0.98
177_Q 209_T 1.315 0.98
221_D 225_R 1.31 0.98
153_A 156_C 1.303 0.98
93_R 100_K 1.298 0.98
275_L 294_F 1.288 0.98
177_Q 256_G 1.286 0.98
199_L 324_I 1.286 0.98
175_V 247_L 1.284 0.98
195_V 198_A 1.264 0.98
173_Y 205_Q 1.258 0.97
275_L 318_I 1.253 0.97
254_F 265_A 1.251 0.97
81_N 88_V 1.249 0.97
100_K 118_T 1.238 0.97
189_N 223_I 1.238 0.97
11_T 41_N 1.228 0.97
78_L 101_I 1.226 0.97
186_C 257_V 1.214 0.97
28_M 38_L 1.209 0.97
130_E 287_W 1.209 0.97
187_W 320_A 1.207 0.97
176_I 255_I 1.205 0.96
196_I 208_L 1.201 0.96
327_T 331_L 1.189 0.96
319_P 322_D 1.185 0.96
40_E 242_P 1.181 0.96
79_V 95_L 1.181 0.96
221_D 234_G 1.177 0.96
3_Y 7_M 1.177 0.96
315_L 318_I 1.173 0.96
194_K 321_E 1.17 0.96
177_Q 259_S 1.166 0.96
288_T 291_I 1.165 0.96
209_T 251_A 1.163 0.95
99_M 119_H 1.159 0.95
134_S 137_E 1.154 0.95
326_A 329_K 1.142 0.95
197_D 201_Q 1.142 0.95
187_W 195_V 1.138 0.95
312_K 315_L 1.121 0.94
15_S 19_Q 1.119 0.94
191_K 319_P 1.114 0.94
96_P 100_K 1.109 0.94
190_D 222_E 1.103 0.93
194_K 320_A 1.103 0.93
15_S 43_E 1.102 0.93
251_A 270_T 1.095 0.93
4_H 34_T 1.093 0.93
178_P 208_L 1.092 0.93
220_V 234_G 1.091 0.93
180_A 186_C 1.091 0.93
273_I 326_A 1.089 0.93
322_D 326_A 1.087 0.93
248_I 268_V 1.085 0.93
179_T 257_V 1.084 0.93
14_I 28_M 1.083 0.93
3_Y 181_R 1.077 0.92
256_G 274_S 1.073 0.92
21_Y 24_A 1.07 0.92
7_M 34_T 1.07 0.92
195_V 320_A 1.055 0.91
3_Y 9_L 1.052 0.91
299_Y 302_M 1.051 0.91
214_A 218_A 1.048 0.91
154_E 158_K 1.047 0.91
188_D 316_S 1.039 0.91
32_Q 51_S 1.034 0.90
16_T 20_N 1.032 0.90
79_V 92_V 1.032 0.90
30_L 47_L 1.029 0.90
273_I 327_T 1.029 0.90
199_L 327_T 1.028 0.90
200_Q 231_P 1.025 0.90
198_A 320_A 1.025 0.90
193_S 227_C 1.016 0.89
196_I 223_I 1.016 0.89
255_I 273_I 1.012 0.89
224_A 234_G 1.005 0.88
222_E 225_R 1 0.88
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1pswA10.94481000.308Contact Map0.747
3tovA20.9391000.309Contact Map0.76
2gt1A20.90411000.345Contact Map0.761
3ot5A40.959398.90.849Contact Map0.509
4hwgA10.953598.50.866Contact Map0.442
2yjnA10.930298.40.87Contact Map0.492
4fzrA10.895398.20.878Contact Map0.634
1v4vA20.892498.20.879Contact Map0.578
1vgvA40.9128980.885Contact Map0.694
3otgA10.8983980.885Contact Map0.603
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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