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OPENSEQ.org

FECI - Probable RNA polymerase sigma factor FecI
UniProt: P23484 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10291
Length: 173 (159)
Sequences: 13714
Seq/Len: 86.25

FECI
Paralog alert: 0.66 [within 20: 0.14] - ratio of genomes with paralogs
Cluster includes: FECI RPOE
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
48_R 72_V 3.141 1.00
132_S 142_I 2.612 1.00
23_W 62_R 2.436 1.00
140_S 150_I 2.359 1.00
125_T 163_H 2.205 1.00
28_L 69_A 2.159 1.00
137_L 145_K 2.076 1.00
124_K 156_Y 2.04 1.00
45_T 69_A 1.998 1.00
70_K 74_V 1.951 1.00
149_S 152_S 1.925 1.00
36_F 39_D 1.869 1.00
151_S 154_K 1.84 1.00
34_S 37_D 1.768 1.00
31_K 73_M 1.748 1.00
125_T 156_Y 1.688 1.00
148_V 156_Y 1.642 1.00
27_W 66_C 1.629 1.00
37_D 76_L 1.621 1.00
75_D 78_R 1.616 1.00
158_A 162_E 1.593 1.00
49_V 65_L 1.591 1.00
24_L 42_A 1.587 1.00
152_S 156_Y 1.585 1.00
139_Y 150_I 1.584 1.00
139_Y 157_V 1.582 1.00
14_E 18_G 1.567 1.00
60_D 63_S 1.558 1.00
138_T 141_E 1.557 1.00
122_N 125_T 1.551 1.00
133_Q 161_V 1.536 1.00
131_L 145_K 1.534 1.00
67_T 71_R 1.521 1.00
128_A 157_V 1.519 1.00
29_T 35_A 1.512 1.00
126_R 130_L 1.496 1.00
67_T 70_K 1.49 1.00
139_Y 154_K 1.485 1.00
72_V 75_D 1.476 1.00
24_L 69_A 1.46 1.00
15_S 19_T 1.442 1.00
32_L 41_I 1.442 1.00
152_S 155_K 1.414 1.00
71_R 74_V 1.351 0.99
44_D 72_V 1.343 0.99
41_I 76_L 1.33 0.99
128_A 156_Y 1.319 0.99
150_I 154_K 1.307 0.99
48_R 75_D 1.304 0.99
35_A 39_D 1.296 0.99
140_S 144_H 1.292 0.99
23_W 66_C 1.28 0.99
28_L 73_M 1.259 0.99
59_R 63_S 1.257 0.99
17_Y 46_F 1.255 0.99
137_L 141_E 1.246 0.98
25_K 29_T 1.229 0.98
28_L 41_I 1.224 0.98
155_K 158_A 1.221 0.98
41_I 72_V 1.209 0.98
25_K 39_D 1.205 0.98
45_T 68_I 1.189 0.98
131_L 137_L 1.181 0.98
148_V 153_V 1.178 0.98
47_L 51_V 1.164 0.97
25_K 35_A 1.158 0.97
119_D 126_R 1.154 0.97
45_T 72_V 1.146 0.97
117_M 164_C 1.143 0.97
142_I 153_V 1.139 0.97
20_H 46_F 1.132 0.97
9_A 12_T 1.131 0.97
123_G 127_E 1.125 0.97
106_S 109_E 1.121 0.97
125_T 160_A 1.12 0.96
74_V 77_F 1.12 0.96
27_W 70_K 1.119 0.96
62_R 66_C 1.114 0.96
24_L 45_T 1.113 0.96
28_L 42_A 1.112 0.96
132_S 139_Y 1.112 0.96
157_V 161_V 1.111 0.96
13_F 50_M 1.11 0.96
17_Y 21_H 1.104 0.96
32_L 73_M 1.099 0.96
151_S 158_A 1.096 0.96
128_A 160_A 1.092 0.96
155_K 159_K 1.091 0.96
151_S 155_K 1.089 0.96
128_A 148_V 1.073 0.95
73_M 77_F 1.059 0.95
49_V 64_F 1.048 0.94
20_H 62_R 1.046 0.94
20_H 65_L 1.046 0.94
139_Y 153_V 1.04 0.94
37_D 80_N 1.028 0.94
125_T 128_A 1.015 0.93
129_F 133_Q 1.014 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1or7A20.98841000.184Contact Map0.843
3vdoA10.98841000.19Contact Map0.822
2q1zA20.95951000.206Contact Map0.494
3n0rA10.88441000.238Contact Map0.707
1rp3A40.98271000.254Contact Map0.689
2lfwA10.83821000.265Contact Map0.639
1l0oC10.95381000.27Contact Map
4kn7X211000.273Contact Map0.535
4ljzF211000.277Contact Map0.559
2a6hF2199.90.335Contact Map0.475

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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