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OPENSEQ.org

RPOE - RNA polymerase sigma-E factor
UniProt: P0AGB6 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11897
Length: 191 (182)
Sequences: 13420
Seq/Len: 73.74

RPOE
Paralog alert: 0.65 [within 20: 0.13] - ratio of genomes with paralogs
Cluster includes: FECI RPOE
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
56_K 81_T 3.295 1.00
149_R 159_I 2.736 1.00
32_K 71_Y 2.516 1.00
157_E 167_V 2.45 1.00
37_V 78_A 2.324 1.00
142_L 180_A 2.287 1.00
137_S 184_K 2.234 1.00
166_P 169_T 2.124 1.00
154_L 162_I 2.12 1.00
53_A 78_A 2.095 1.00
60_A 63_S 2.057 1.00
141_D 173_R 2.047 1.00
79_V 83_K 1.96 1.00
168_G 171_R 1.95 1.00
44_G 47_P 1.922 1.00
40_Y 82_A 1.856 1.00
42_P 45_D 1.853 1.00
134_T 184_K 1.81 1.00
36_L 75_Y 1.724 1.00
45_D 85_Y 1.713 1.00
142_L 173_R 1.711 1.00
84_N 87_V 1.703 1.00
13_R 18_D 1.696 1.00
33_V 50_V 1.686 1.00
145_A 174_I 1.67 1.00
169_T 173_R 1.67 1.00
165_C 173_R 1.665 1.00
156_Y 174_I 1.659 1.00
23_N 27_V 1.653 1.00
57_A 74_L 1.631 1.00
69_A 72_T 1.631 1.00
76_R 80_N 1.63 1.00
150_E 178_R 1.627 1.00
156_Y 167_V 1.607 1.00
38_S 43_S 1.606 1.00
155_S 158_E 1.584 1.00
156_Y 171_R 1.583 1.00
76_R 79_V 1.577 1.00
175_F 179_E 1.573 1.00
81_T 84_N 1.561 1.00
139_P 142_L 1.558 1.00
143_R 147_T 1.535 1.00
169_T 172_S 1.528 1.00
148_L 162_I 1.527 1.00
33_V 78_A 1.518 1.00
41_V 49_V 1.49 1.00
180_A 184_K 1.473 1.00
56_K 84_N 1.407 0.99
157_E 161_A 1.401 0.99
24_L 28_R 1.392 0.99
49_V 85_Y 1.389 0.99
80_N 83_K 1.381 0.99
145_A 173_R 1.36 0.99
52_E 81_T 1.359 0.99
37_V 82_A 1.319 0.99
43_S 47_P 1.315 0.99
26_V 54_F 1.304 0.99
68_S 72_T 1.297 0.99
167_V 171_R 1.294 0.99
49_V 81_T 1.292 0.99
37_V 49_V 1.289 0.99
34_A 38_S 1.278 0.99
7_D 28_R 1.251 0.99
154_L 158_E 1.234 0.98
32_K 75_Y 1.223 0.98
34_A 47_P 1.218 0.98
165_C 170_V 1.217 0.98
29_Y 54_F 1.213 0.98
149_R 156_Y 1.195 0.98
37_V 50_V 1.188 0.98
174_I 178_R 1.187 0.98
159_I 170_V 1.185 0.98
53_A 77_I 1.183 0.98
34_A 43_S 1.179 0.98
148_L 154_L 1.178 0.98
41_V 82_A 1.17 0.97
36_L 79_V 1.161 0.97
172_S 175_F 1.161 0.97
53_A 81_T 1.159 0.97
168_G 175_F 1.15 0.97
136_E 143_R 1.143 0.97
33_V 53_A 1.141 0.97
172_S 176_R 1.14 0.97
140_E 144_M 1.14 0.97
145_A 177_A 1.14 0.97
57_A 73_W 1.138 0.97
156_Y 170_V 1.136 0.97
134_T 181_I 1.127 0.97
26_V 30_Q 1.126 0.97
142_L 177_A 1.121 0.97
22_F 58_Y 1.117 0.96
71_Y 75_Y 1.115 0.96
123_L 126_E 1.11 0.96
168_G 172_S 1.106 0.96
83_K 86_L 1.105 0.96
29_Y 74_L 1.103 0.96
145_A 165_C 1.094 0.96
82_A 86_L 1.084 0.96
55_I 59_R 1.075 0.95
29_Y 71_Y 1.064 0.95
45_D 89_Q 1.056 0.95
38_S 46_V 1.055 0.95
64_F 68_S 1.013 0.93
25_L 70_F 1.011 0.93
142_L 145_A 1.011 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1or7A20.99481000.115Contact Map0.831
3vdoA10.94761000.167Contact Map0.804
2q1zA20.91621000.187Contact Map0.494
4kn7X20.98951000.257Contact Map0.508
3n0rA10.85341000.263Contact Map0.678
1l0oC10.94761000.265Contact Map
4ljzF20.98951000.266Contact Map0.528
1rp3A40.93191000.266Contact Map0.707
2lfwA10.76441000.268Contact Map0.616
2a6hF20.989599.90.335Contact Map0.455

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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