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OPENSEQ.org

INSI1 - Transposase InsI for insertion sequence element IS30A
UniProt: P0CF88 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG40009
Length: 383 (360)
Sequences: 638
Seq/Len: 1.77

INSI1
Paralog alert: 0.45 [within 20: 0.05] - ratio of genomes with paralogs
Cluster includes: INSI1 INSI3 INSI4
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
316_D 322_Q 4.53 1.00
84_L 91_I 4.121 1.00
337_F 354_V 3.958 1.00
78_R 88_P 3.93 1.00
255_S 374_I 3.857 1.00
71_L 95_V 3.202 1.00
230_S 233_H 3.183 1.00
63_E 86_R 3.071 1.00
219_H 316_D 2.993 1.00
323_R 326_N 2.873 1.00
146_E 364_K 2.87 1.00
256_R 365_T 2.846 1.00
219_H 322_Q 2.479 1.00
260_I 333_I 2.232 0.99
228_R 256_R 2.22 0.99
150_G 368_F 2.203 0.99
247_I 337_F 2.172 0.99
236_G 292_W 2.156 0.99
348_Q 352_D 2.104 0.99
110_N 114_N 2.098 0.99
151_W 373_E 2.092 0.99
260_I 355_A 2.017 0.99
89_S 93_R 1.978 0.98
251_V 258_T 1.932 0.98
228_R 325_T 1.817 0.97
256_R 325_T 1.805 0.97
231_L 252_D 1.758 0.96
66_E 70_G 1.725 0.96
104_Y 109_A 1.678 0.95
87_S 92_S 1.669 0.94
70_G 95_V 1.653 0.94
77_I 92_S 1.652 0.94
225_I 252_D 1.651 0.94
366_L 370_T 1.65 0.94
59_L 67_I 1.646 0.94
79_A 82_T 1.635 0.94
335_Q 361_R 1.634 0.94
75_M 83_A 1.624 0.93
247_I 351_L 1.617 0.93
88_P 92_S 1.585 0.92
370_T 373_E 1.569 0.92
267_D 270_S 1.569 0.92
215_G 294_R 1.553 0.91
225_I 289_S 1.548 0.91
142_K 357_Q 1.545 0.91
146_E 368_F 1.539 0.91
234_W 252_D 1.526 0.90
208_G 296_M 1.516 0.90
301_H 305_T 1.51 0.90
336_Y 357_Q 1.509 0.90
60_T 86_R 1.508 0.90
92_S 96_Q 1.501 0.89
167_E 171_K 1.495 0.89
68_R 106_A 1.491 0.89
75_M 79_A 1.488 0.89
59_L 90_T 1.485 0.89
70_G 83_A 1.47 0.88
214_N 302_L 1.46 0.87
300_R 303_E 1.446 0.87
79_A 83_A 1.43 0.86
143_W 366_L 1.392 0.84
217_P 316_D 1.387 0.84
265_G 270_S 1.381 0.83
369_K 374_I 1.372 0.83
236_G 250_L 1.355 0.82
59_L 64_R 1.348 0.81
239_V 260_I 1.34 0.81
213_V 299_A 1.317 0.79
98_N 116_M 1.306 0.78
315_C 323_R 1.305 0.78
332_L 361_R 1.294 0.77
258_T 329_T 1.289 0.77
366_L 371_P 1.289 0.77
89_S 92_S 1.289 0.77
292_W 323_R 1.286 0.77
275_L 304_F 1.285 0.77
221_R 291_T 1.279 0.76
228_R 253_R 1.268 0.75
326_N 330_N 1.263 0.75
64_R 67_I 1.245 0.73
63_E 84_L 1.239 0.73
256_R 366_L 1.235 0.72
359_N 362_P 1.23 0.72
333_I 337_F 1.229 0.72
24_S 34_P 1.225 0.72
262_R 348_Q 1.208 0.70
247_I 260_I 1.206 0.70
240_S 266_K 1.199 0.69
134_L 138_K 1.198 0.69
250_L 271_V 1.193 0.69
77_I 86_R 1.179 0.67
151_W 370_T 1.176 0.67
364_K 368_F 1.166 0.66
65_E 69_A 1.163 0.66
333_I 354_V 1.144 0.64
263_L 270_S 1.133 0.63
253_R 256_R 1.125 0.62
26_I 40_M 1.116 0.61
248_A 261_V 1.11 0.60
135_V 169_I 1.108 0.60
325_T 329_T 1.108 0.60
294_R 318_Q 1.106 0.60
26_I 37_I 1.106 0.60
26_I 32_S 1.099 0.59
147_Q 335_Q 1.096 0.59
359_N 363_R 1.095 0.59
77_I 88_P 1.094 0.59
356_A 360_N 1.086 0.58
328_N 331_G 1.083 0.58
84_L 88_P 1.078 0.57
76_S 79_A 1.074 0.57
339_K 359_N 1.068 0.56
70_G 75_M 1.064 0.56
362_P 371_P 1.062 0.55
319_S 322_Q 1.061 0.55
251_V 329_T 1.061 0.55
292_W 298_L 1.06 0.55
248_A 263_L 1.059 0.55
313_Y 333_I 1.059 0.55
359_N 371_P 1.059 0.55
145_P 148_I 1.053 0.54
25_E 28_N 1.052 0.54
241_G 250_L 1.051 0.54
147_Q 196_R 1.05 0.54
252_D 289_S 1.05 0.54
257_Y 372_K 1.047 0.54
141_M 357_Q 1.046 0.54
332_L 335_Q 1.044 0.53
21_T 30_L 1.042 0.53
343_L 346_Y 1.04 0.53
224_N 230_S 1.04 0.53
26_I 30_L 1.033 0.52
248_A 271_V 1.032 0.52
369_K 373_E 1.032 0.52
115_R 118_K 1.032 0.52
145_P 169_I 1.031 0.52
30_L 34_P 1.03 0.52
258_T 333_I 1.028 0.52
251_V 325_T 1.023 0.51
151_W 155_T 1.014 0.50
76_S 88_P 1.014 0.50
21_T 24_S 1.013 0.50
316_D 319_S 1.007 0.49
82_T 255_S 1.006 0.49
262_R 346_Y 1 0.49
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4fcyA20.82771000.721Contact Map0.336
3kksA20.386499.90.829Contact Map0.623
3av9A20.462199.90.834Contact Map0.608
3l3uA20.409999.90.834Contact Map0.61
1ex4A20.409999.90.834Contact Map0.553
1c6vA40.409999.90.836Contact Map0.691
4fw1A20.417899.90.837Contact Map0.486
1cxqA10.38999.80.848Contact Map0.685
3f9kA240.535299.80.85Contact Map0.601
2x78A30.420499.80.851Contact Map0.581

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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