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OPENSEQ.org
INSI4 - Transposase InsI for insertion sequence element IS30D
UniProt: P0CF90 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG60051
Length: 383 (360)
Sequences: 640
Seq/Len: 1.78
INSI4
Paralog alert: 0.45 [within 20: 0.05] - ratio of genomes with paralogs
Cluster includes: INSI1 INSI3 INSI4
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
316_D 322_Q 4.561 1.00
84_L 91_I 4.169 1.00
337_F 354_V 3.97 1.00
78_R 88_P 3.885 1.00
255_S 374_I 3.849 1.00
71_L 95_V 3.195 1.00
230_S 233_H 3.177 1.00
63_E 86_R 3.094 1.00
219_H 316_D 2.975 1.00
323_R 326_N 2.869 1.00
146_E 364_K 2.842 1.00
256_R 365_T 2.84 1.00
219_H 322_Q 2.482 1.00
260_I 333_I 2.238 1.00
228_R 256_R 2.221 0.99
150_G 368_F 2.198 0.99
247_I 337_F 2.177 0.99
236_G 292_W 2.144 0.99
110_N 114_N 2.122 0.99
151_W 373_E 2.115 0.99
260_I 355_A 2.018 0.99
348_Q 352_D 2.017 0.99
89_S 93_R 1.983 0.99
251_V 258_T 1.928 0.98
228_R 325_T 1.805 0.97
256_R 325_T 1.776 0.96
231_L 252_D 1.77 0.96
87_S 92_S 1.765 0.96
104_Y 109_A 1.728 0.96
247_I 351_L 1.719 0.96
66_E 70_G 1.715 0.95
59_L 67_I 1.687 0.95
77_I 92_S 1.656 0.94
79_A 82_T 1.642 0.94
225_I 252_D 1.632 0.94
75_M 83_A 1.63 0.94
370_T 373_E 1.624 0.93
335_Q 361_R 1.622 0.93
366_L 370_T 1.62 0.93
88_P 92_S 1.609 0.93
70_G 95_V 1.599 0.93
146_E 368_F 1.549 0.91
215_G 294_R 1.543 0.91
225_I 289_S 1.539 0.91
142_K 357_Q 1.53 0.90
267_D 270_S 1.52 0.90
234_W 252_D 1.519 0.90
59_L 90_T 1.517 0.90
208_G 296_M 1.509 0.90
336_Y 357_Q 1.506 0.90
92_S 96_Q 1.502 0.89
60_T 86_R 1.494 0.89
167_E 171_K 1.494 0.89
68_R 106_A 1.483 0.89
301_H 305_T 1.474 0.88
70_G 83_A 1.462 0.88
75_M 79_A 1.456 0.87
214_N 302_L 1.431 0.86
300_R 303_E 1.423 0.86
79_A 83_A 1.394 0.84
369_K 374_I 1.394 0.84
217_P 316_D 1.388 0.84
143_W 366_L 1.385 0.84
364_K 368_F 1.378 0.83
359_N 362_P 1.349 0.81
236_G 250_L 1.347 0.81
265_G 270_S 1.342 0.81
366_L 371_P 1.34 0.81
239_V 260_I 1.335 0.80
315_C 323_R 1.311 0.79
213_V 299_A 1.305 0.78
332_L 361_R 1.298 0.78
258_T 329_T 1.296 0.78
292_W 323_R 1.286 0.77
64_R 67_I 1.282 0.76
98_N 116_M 1.281 0.76
221_R 291_T 1.28 0.76
326_N 330_N 1.274 0.76
59_L 64_R 1.258 0.75
228_R 253_R 1.258 0.75
247_I 260_I 1.236 0.73
89_S 92_S 1.234 0.72
63_E 84_L 1.23 0.72
333_I 337_F 1.227 0.72
256_R 366_L 1.22 0.71
24_S 34_P 1.218 0.71
134_L 138_K 1.211 0.70
240_S 266_K 1.207 0.70
262_R 348_Q 1.204 0.70
151_W 370_T 1.202 0.70
250_L 271_V 1.192 0.69
77_I 86_R 1.184 0.68
65_E 69_A 1.174 0.67
275_L 304_F 1.17 0.67
359_N 363_R 1.168 0.66
333_I 354_V 1.162 0.66
26_I 40_M 1.123 0.62
263_L 270_S 1.12 0.62
253_R 256_R 1.119 0.62
359_N 371_P 1.118 0.61
362_P 371_P 1.112 0.61
77_I 88_P 1.11 0.61
26_I 37_I 1.108 0.60
294_R 318_Q 1.103 0.60
26_I 32_S 1.103 0.60
325_T 329_T 1.102 0.60
369_K 373_E 1.097 0.59
135_V 169_I 1.094 0.59
84_L 88_P 1.093 0.59
77_I 95_V 1.089 0.58
147_Q 335_Q 1.088 0.58
248_A 261_L 1.085 0.58
70_G 75_M 1.082 0.58
205_G 209_T 1.081 0.58
94_E 97_R 1.076 0.57
356_A 360_N 1.075 0.57
319_S 322_Q 1.072 0.57
252_D 289_S 1.068 0.56
332_L 335_Q 1.068 0.56
248_A 263_L 1.068 0.56
292_W 298_L 1.062 0.56
328_N 331_G 1.057 0.55
251_V 329_T 1.056 0.55
147_Q 196_R 1.054 0.55
313_Y 333_I 1.051 0.54
145_P 148_I 1.05 0.54
25_E 28_N 1.048 0.54
241_G 250_L 1.046 0.54
224_N 230_S 1.045 0.54
141_M 357_Q 1.043 0.54
76_S 79_A 1.039 0.53
151_W 155_T 1.039 0.53
21_T 30_L 1.038 0.53
257_Y 372_K 1.034 0.53
145_P 169_I 1.032 0.52
26_I 30_L 1.032 0.52
258_T 333_I 1.03 0.52
82_T 255_S 1.028 0.52
30_L 34_P 1.028 0.52
362_P 370_T 1.021 0.51
352_D 356_A 1.019 0.51
251_V 325_T 1.013 0.50
76_S 92_S 1.012 0.50
76_S 88_P 1.012 0.50
222_S 228_R 1.01 0.50
248_A 271_V 1.008 0.50
21_T 24_S 1.008 0.50
316_D 319_S 1.004 0.49
304_F 308_T 1.003 0.49
262_R 346_Y 1.003 0.49
359_N 366_L 1.002 0.49
343_L 346_Y 1.002 0.49
32_S 40_M 1.002 0.49
151_W 154_R 1.001 0.49
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4fcyA20.82771000.721Contact Map0.338
3kksA20.386499.90.829Contact Map0.61
3av9A20.462199.90.834Contact Map0.605
3l3uA20.409999.90.834Contact Map0.6
1ex4A20.409999.90.834Contact Map0.543
1c6vA40.409999.90.836Contact Map0.674
4fw1A20.417899.90.837Contact Map0.492
1cxqA10.38999.80.849Contact Map0.683
3f9kA240.537999.80.849Contact Map0.593
2x78A30.420499.80.851Contact Map0.58
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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