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OPENSEQ.org

PBP7 - D-alanyl-D-alanine endopeptidase
UniProt: P0AFI5 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12015
Length: 310 (250)
Sequences: 1731
Seq/Len: 6.92

PBP7
Paralog alert: 0.53 [within 20: 0.02] - ratio of genomes with paralogs
Cluster includes: DACA DACC DACD PBP7
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
60_D 176_R 3.845 1.00
113_R 132_L 3.699 1.00
79_D 150_K 3.183 1.00
56_S 59_P 3.038 1.00
119_L 125_E 2.907 1.00
137_P 146_A 2.605 1.00
77_V 132_L 2.525 1.00
150_K 153_S 2.501 1.00
87_K 110_E 2.496 1.00
120_A 129_A 2.462 1.00
43_A 55_Y 2.429 1.00
156_M 181_L 2.406 1.00
47_D 251_K 2.386 1.00
42_S 175_A 2.378 1.00
91_D 134_H 2.32 1.00
112_S 115_D 2.309 1.00
60_D 158_N 2.283 1.00
182_L 230_T 2.277 1.00
72_M 159_T 2.258 1.00
75_M 184_A 2.215 1.00
85_D 114_K 2.136 1.00
86_E 135_H 2.048 1.00
62_V 172_V 2.022 1.00
74_A 121_L 2.017 1.00
42_S 63_R 1.985 1.00
160_R 172_V 1.972 1.00
45_I 253_V 1.968 1.00
71_L 243_V 1.968 1.00
154_L 184_A 1.964 1.00
68_I 178_L 1.962 1.00
228_Q 252_P 1.901 1.00
228_Q 245_R 1.898 1.00
120_A 132_L 1.895 1.00
48_L 254_A 1.891 1.00
68_I 243_V 1.884 1.00
140_Y 164_P 1.836 1.00
137_P 142_A 1.797 1.00
184_A 187_Q 1.783 1.00
77_V 113_R 1.751 1.00
68_I 258_M 1.742 1.00
58_H 61_L 1.732 1.00
176_R 180_K 1.717 1.00
72_M 151_A 1.682 1.00
255_L 270_A 1.669 1.00
235_T 238_A 1.658 1.00
118_L 122_M 1.636 1.00
90_V 131_S 1.614 1.00
175_A 258_M 1.605 1.00
65_I 173_S 1.591 1.00
88_L 131_S 1.588 1.00
84_L 117_L 1.557 1.00
62_V 158_N 1.555 1.00
159_T 173_S 1.55 0.99
238_A 241_C 1.52 0.99
226_N 248_I 1.499 0.99
87_K 112_S 1.472 0.99
247_V 250_N 1.466 0.99
118_L 197_T 1.463 0.99
89_K 110_E 1.455 0.99
139_G 142_A 1.454 0.99
73_T 129_A 1.447 0.99
244_M 270_A 1.442 0.99
182_L 243_V 1.413 0.99
142_A 145_K 1.403 0.99
46_V 179_T 1.397 0.99
41_G 259_D 1.374 0.98
83_P 86_E 1.373 0.98
44_M 59_P 1.37 0.98
190_L 193_Q 1.365 0.98
43_A 57_N 1.36 0.98
178_L 243_V 1.335 0.98
94_Q 134_H 1.335 0.98
247_V 252_P 1.33 0.98
75_M 150_K 1.328 0.98
74_A 120_A 1.313 0.98
114_K 118_L 1.301 0.98
72_M 76_V 1.286 0.97
75_M 188_Y 1.27 0.97
180_K 183_I 1.256 0.97
179_T 254_A 1.246 0.97
119_L 128_A 1.236 0.97
84_L 114_K 1.202 0.96
148_N 161_F 1.201 0.96
88_L 135_H 1.199 0.96
64_P 168_S 1.197 0.96
158_N 174_T 1.176 0.95
140_Y 144_I 1.15 0.94
136_Y 146_A 1.146 0.94
245_R 252_P 1.142 0.94
42_S 58_H 1.136 0.94
257_V 270_A 1.129 0.93
91_D 95_T 1.126 0.93
124_S 231_K 1.12 0.93
151_A 156_M 1.114 0.93
168_S 237_A 1.109 0.93
223_D 275_T 1.104 0.92
195_S 230_T 1.097 0.92
192_G 252_P 1.095 0.92
74_A 117_L 1.089 0.92
62_V 174_T 1.082 0.91
76_V 147_M 1.077 0.91
269_D 272_R 1.077 0.91
72_M 161_F 1.064 0.90
114_K 194_L 1.059 0.90
116_M 128_A 1.058 0.90
49_N 252_P 1.057 0.90
163_E 168_S 1.054 0.90
145_K 149_A 1.051 0.90
142_A 146_A 1.049 0.90
192_G 195_S 1.044 0.89
151_A 181_L 1.036 0.89
125_E 128_A 1.034 0.89
76_V 79_D 1.034 0.89
71_L 185_S 1.033 0.89
246_T 274_R 1.027 0.88
44_M 179_T 1.024 0.88
253_V 277_I 1.024 0.88
111_I 116_M 1.01 0.87
144_I 147_M 1.001 0.86
45_I 255_L 1 0.86
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3mfdA20.86451000.373Contact Map0.845
3itaA40.89351000.387Contact Map0.853
3mzfA10.90651000.39Contact Map0.846
4k91A20.87421000.392Contact Map0.856
1xp4A40.89351000.397Contact Map0.903
3a3jA10.88061000.397Contact Map0.857
3hunA20.9711000.407Contact Map0.877
1tvfA20.92581000.424Contact Map0.879
2bcfA10.8711000.453Contact Map0.836
1es5A10.79681000.456Contact Map0.842

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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