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MIAB - (Dimethylallyl)adenosine tRNA methylthiotransferase MiaB
UniProt: P0AEI1 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13657
Length: 474 (436)
Sequences: 3596
Seq/Len: 8.25
MIAB
Paralog alert: 0.79 [within 20: 0.04] - ratio of genomes with paralogs
Cluster includes: MIAB RIMO
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
270_S 317_D 4.428 1.00
189_A 231_I 4.312 1.00
271_D 284_L 4.277 1.00
270_S 320_K 3.991 1.00
151_V 185_I 3.908 1.00
329_N 375_A 3.764 1.00
426_E 438_K 3.618 1.00
326_A 371_Q 3.473 1.00
384_T 424_D 3.473 1.00
246_D 292_K 3.446 1.00
386_R 424_D 3.402 1.00
389_V 412_F 3.188 1.00
323_K 327_D 3.146 1.00
272_R 317_D 3.058 1.00
360_Q 364_I 3.049 1.00
250_E 296_A 2.976 1.00
272_R 276_L 2.949 1.00
287_K 327_D 2.9 1.00
226_A 255_T 2.893 1.00
428_T 438_K 2.69 1.00
183_F 186_A 2.672 1.00
262_L 300_I 2.621 1.00
185_I 225_V 2.586 1.00
185_I 196_V 2.578 1.00
224_L 227_A 2.515 1.00
250_E 254_D 2.508 1.00
322_M 368_R 2.414 1.00
384_T 426_E 2.411 1.00
288_A 291_R 2.409 1.00
223_R 254_D 2.408 1.00
186_A 190_A 2.4 1.00
318_F 364_I 2.388 1.00
287_K 328_V 2.367 1.00
323_K 326_A 2.276 1.00
293_L 300_I 2.274 1.00
110_R 113_E 2.266 1.00
315_T 319_E 2.234 1.00
357_E 360_Q 2.225 1.00
386_R 422_F 2.199 1.00
326_A 368_R 2.18 1.00
9_T 19_S 2.151 1.00
319_E 323_K 2.141 1.00
315_T 357_E 2.131 1.00
260_S 405_E 2.126 1.00
386_R 405_E 2.116 1.00
194_R 232_D 2.06 1.00
283_A 320_K 2.054 1.00
160_Y 172_E 2.044 1.00
340_A 352_D 2.013 1.00
381_L 430_V 2.007 1.00
317_D 320_K 2.001 1.00
354_P 357_E 1.975 1.00
217_S 220_D 1.971 1.00
329_N 372_Q 1.963 1.00
217_S 247_D 1.96 1.00
291_R 327_D 1.949 1.00
268_S 321_T 1.947 1.00
319_E 364_I 1.891 1.00
387_I 402_G 1.873 1.00
337_I 362_L 1.868 1.00
374_M 378_R 1.867 1.00
4_K 33_Q 1.852 1.00
37_V 40_E 1.841 1.00
7_I 19_S 1.838 1.00
262_L 293_L 1.835 1.00
226_A 257_E 1.796 1.00
285_E 288_A 1.796 1.00
355_E 358_K 1.776 1.00
271_D 283_A 1.766 1.00
283_A 287_K 1.737 1.00
291_R 295_A 1.734 1.00
316_E 320_K 1.723 1.00
266_V 324_L 1.721 1.00
273_I 276_L 1.703 1.00
178_S 224_L 1.673 1.00
223_R 247_D 1.66 1.00
380_M 385_Q 1.655 1.00
284_L 288_A 1.626 1.00
249_I 293_L 1.6 1.00
331_D 376_W 1.595 1.00
253_R 296_A 1.595 1.00
389_V 423_V 1.594 1.00
315_T 364_I 1.591 1.00
376_W 379_R 1.58 1.00
246_D 250_E 1.575 1.00
315_T 360_Q 1.572 1.00
322_M 364_I 1.566 1.00
85_A 102_I 1.558 1.00
282_T 285_E 1.547 1.00
162_T 310_F 1.546 1.00
176_R 184_E 1.546 1.00
162_T 278_G 1.541 1.00
260_S 299_D 1.541 1.00
380_M 383_T 1.528 1.00
50_S 169_T 1.528 1.00
320_K 323_K 1.523 1.00
189_A 228_I 1.519 1.00
226_A 256_P 1.491 1.00
288_A 292_K 1.486 1.00
283_A 324_L 1.484 1.00
283_A 323_K 1.482 0.99
359_K 363_Y 1.475 0.99
232_D 386_R 1.467 0.99
223_R 255_T 1.458 0.99
418_M 421_K 1.448 0.99
276_L 317_D 1.438 0.99
299_D 383_T 1.432 0.99
316_E 319_E 1.42 0.99
381_L 429_D 1.397 0.99
292_K 295_A 1.395 0.99
225_V 234_I 1.394 0.99
222_L 258_L 1.386 0.99
226_A 251_V 1.378 0.99
410_V 427_I 1.373 0.99
299_D 379_R 1.366 0.99
335_S 362_L 1.365 0.99
276_L 310_F 1.365 0.99
53_E 57_E 1.361 0.99
329_N 376_W 1.357 0.99
90_E 94_Q 1.343 0.99
45_L 79_G 1.328 0.99
251_V 255_T 1.319 0.99
306_F 325_I 1.308 0.98
314_T 317_D 1.3 0.98
290_I 302_I 1.295 0.98
223_R 251_V 1.294 0.98
425_V 437_G 1.288 0.98
252_Y 258_L 1.288 0.98
183_F 187_Q 1.282 0.98
218_F 248_I 1.272 0.98
177_P 180_D 1.267 0.98
68_L 71_E 1.265 0.98
378_R 381_L 1.264 0.98
29_T 116_N 1.26 0.98
355_E 359_K 1.254 0.98
271_D 320_K 1.249 0.98
340_A 347_A 1.247 0.98
182_L 186_A 1.243 0.98
88_E 91_H 1.239 0.98
165_V 169_T 1.238 0.98
79_G 101_I 1.231 0.97
160_Y 168_Y 1.229 0.97
22_M 81_G 1.224 0.97
4_K 40_E 1.223 0.97
186_A 229_D 1.215 0.97
363_Y 367_E 1.209 0.97
378_R 430_V 1.206 0.97
196_V 231_I 1.205 0.97
5_L 34_L 1.204 0.97
229_D 257_E 1.203 0.97
220_D 247_D 1.2 0.97
325_I 365_L 1.197 0.97
387_I 425_V 1.195 0.97
369_I 372_Q 1.195 0.97
308_V 318_F 1.192 0.97
371_Q 375_A 1.189 0.97
337_I 359_K 1.184 0.97
86_S 104_G 1.181 0.97
301_Q 406_N 1.177 0.96
429_D 436_R 1.174 0.96
182_L 224_L 1.174 0.96
427_I 435_L 1.169 0.96
220_D 224_L 1.165 0.96
329_N 379_R 1.164 0.96
304_S 333_S 1.161 0.96
271_D 282_T 1.146 0.96
367_E 371_Q 1.143 0.96
318_F 365_L 1.139 0.95
176_R 180_D 1.137 0.95
337_I 355_E 1.135 0.95
234_I 257_E 1.133 0.95
232_D 257_E 1.132 0.95
297_R 300_I 1.13 0.95
368_R 371_Q 1.126 0.95
187_Q 191_Q 1.125 0.95
347_A 352_D 1.12 0.95
48_T 80_V 1.118 0.95
368_R 372_Q 1.111 0.95
385_Q 427_I 1.107 0.95
276_L 312_G 1.102 0.94
276_L 349_M 1.093 0.94
335_S 366_Q 1.091 0.94
67_K 78_I 1.091 0.94
278_G 345_P 1.09 0.94
73_N 76_L 1.088 0.94
70_K 100_D 1.088 0.94
252_Y 262_L 1.079 0.93
251_V 258_L 1.077 0.93
319_E 322_M 1.071 0.93
8_K 62_Q 1.056 0.93
151_V 196_V 1.056 0.93
352_D 358_K 1.051 0.92
57_E 61_H 1.051 0.92
253_R 297_R 1.047 0.92
187_Q 190_A 1.044 0.92
203_V 236_F 1.043 0.92
81_G 103_F 1.038 0.92
219_A 251_V 1.037 0.92
370_N 433_N 1.032 0.91
14_M 201_Q 1.028 0.91
255_T 258_L 1.021 0.91
249_I 296_A 1.013 0.90
369_I 373_A 1.008 0.90
325_I 330_F 1.007 0.90
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4jc0A20.91141000.398Contact Map0.704
2qgqA80.63081000.589Contact Map0.811
1oltA10.76581000.802Contact Map0.458
2yx0A10.651999.80.862Contact Map0.465
3iixA10.700499.80.863Contact Map0.587
3c8fA10.487399.80.869Contact Map0.47
2z2uA10.578199.80.869Contact Map0.527
3t7vA10.689999.80.87Contact Map0.482
1r30A20.700499.80.873Contact Map0.518
1tv8A20.649899.70.876Contact Map0.489
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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