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OPENSEQ.org
CYBH - Probable Ni/Fe-hydrogenase 1 B-type cytochrome subunit
UniProt: P0AAM1 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10470
Length: 235 (215)
Sequences: 726
Seq/Len: 3.38
CYBH
Paralog alert: 0.52 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: CYBH FDNI FDOI
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
31_V 66_A 3.514 1.00
25_T 73_V 2.048 1.00
67_G 70_F 1.978 1.00
12_Y 221_Y 1.972 1.00
79_Y 83_V 1.844 0.99
17_P 80_W 1.787 0.99
14_F 125_H 1.67 0.99
99_K 103_Q 1.652 0.98
27_L 214_I 1.641 0.98
31_V 35_T 1.594 0.98
196_I 200_Y 1.569 0.98
35_T 66_A 1.569 0.98
181_H 185_R 1.567 0.98
33_M 188_M 1.563 0.97
30_A 34_V 1.56 0.97
206_D 212_T 1.543 0.97
40_G 185_R 1.534 0.97
37_Y 188_M 1.52 0.97
125_H 129_A 1.481 0.96
26_V 30_A 1.462 0.96
74_L 144_I 1.444 0.95
158_A 167_V 1.429 0.95
17_P 124_G 1.424 0.95
139_M 194_F 1.41 0.95
28_C 70_F 1.394 0.94
133_M 194_F 1.378 0.94
21_W 73_V 1.371 0.94
70_F 140_S 1.37 0.93
179_D 183_W 1.367 0.93
71_T 148_F 1.361 0.93
206_D 215_S 1.358 0.93
52_Y 55_Y 1.348 0.93
217_M 220_G 1.348 0.93
79_Y 200_Y 1.325 0.92
46_V 53_L 1.322 0.92
110_R 116_A 1.318 0.92
186_L 199_V 1.315 0.92
199_V 202_A 1.299 0.91
10_S 221_Y 1.297 0.91
35_T 114_F 1.297 0.91
24_L 73_V 1.29 0.91
212_T 216_T 1.279 0.90
142_F 187_G 1.271 0.90
105_V 140_S 1.269 0.90
182_S 185_R 1.268 0.89
32_L 146_T 1.266 0.89
33_M 192_G 1.265 0.89
78_I 82_F 1.255 0.89
77_R 84_G 1.254 0.89
176_N 218_V 1.246 0.88
35_T 218_V 1.244 0.88
15_E 88_S 1.237 0.88
215_S 219_N 1.236 0.88
54_F 58_Y 1.227 0.87
62_I 65_S 1.221 0.87
64_F 151_Y 1.211 0.86
104_G 137_F 1.208 0.86
78_I 200_Y 1.203 0.86
32_L 36_G 1.195 0.85
129_A 133_M 1.191 0.85
32_L 70_F 1.188 0.85
138_L 195_V 1.184 0.85
15_E 80_W 1.184 0.85
80_W 88_S 1.183 0.85
195_V 199_V 1.176 0.84
80_W 84_G 1.176 0.84
18_V 88_S 1.157 0.83
14_F 77_R 1.147 0.82
70_F 87_Y 1.146 0.82
205_E 217_M 1.139 0.81
143_M 187_G 1.135 0.81
35_T 42_P 1.133 0.81
90_E 108_E 1.131 0.81
32_L 181_H 1.123 0.80
58_Y 62_I 1.123 0.80
98_R 101_W 1.118 0.80
13_V 57_G 1.113 0.79
141_V 197_G 1.113 0.79
78_I 138_L 1.11 0.79
25_T 67_G 1.108 0.79
39_I 150_L 1.107 0.79
88_S 129_A 1.106 0.79
88_S 125_H 1.105 0.79
199_V 214_I 1.104 0.79
30_A 33_M 1.104 0.79
142_F 183_W 1.102 0.78
54_F 57_G 1.094 0.78
200_Y 212_T 1.09 0.77
182_S 186_L 1.076 0.76
14_F 124_G 1.073 0.76
152_S 155_S 1.071 0.76
150_L 177_S 1.067 0.75
204_R 208_M 1.067 0.75
21_W 76_M 1.064 0.75
202_A 214_I 1.064 0.75
18_V 80_W 1.058 0.74
65_S 68_M 1.058 0.74
90_E 125_H 1.058 0.74
146_T 149_A 1.051 0.74
60_R 150_L 1.051 0.74
214_I 217_M 1.05 0.74
128_I 194_F 1.041 0.73
100_S 190_L 1.037 0.72
185_R 189_W 1.033 0.72
56_M 64_F 1.031 0.72
68_M 72_V 1.021 0.71
73_V 76_M 1.018 0.70
152_S 180_I 1.018 0.70
80_W 125_H 1.018 0.70
33_M 36_G 1.017 0.70
43_L 56_M 1.015 0.70
136_Y 140_S 1.014 0.70
98_R 103_Q 1.014 0.70
74_L 140_S 1.013 0.70
47_S 53_L 1.007 0.69
57_G 82_F 1.004 0.69
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4gd3A211000.355Contact Map0.48
1kqfC10.85111000.554Contact Map0.455
2qjyA60.855380.30.929Contact Map0.427
1pp9C20.83466.40.937Contact Map0.427
4h44A10.5617390.946Contact Map0.461
3cx5C20.548931.60.949Contact Map0.399
3dl8E20.39575.50.964Contact Map0.01
1wazA10.1833.30.967Contact Map0.466
1m56D20.17022.40.97Contact Map0.256
3arcI20.16172.10.971Contact Map0.542
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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