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OPENSEQ.org
RF1 - Peptide chain release factor 1
UniProt: P0A7I0 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10761
Length: 360 (353)
Sequences: 2900
Seq/Len: 8.22
RF1
Paralog alert: 0.82 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: RF1 RF2
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
282_E 285_K 4.545 1.00
284_A 288_Q 3.212 1.00
113_L 164_A 3.186 1.00
142_R 338_D 3 1.00
312_F 333_M 2.959 1.00
139_A 166_I 2.848 1.00
280_A 284_A 2.842 1.00
15_R 41_E 2.838 1.00
224_I 246_I 2.828 1.00
227_F 256_E 2.74 1.00
328_R 331_E 2.699 1.00
19_V 38_L 2.599 1.00
222_L 246_I 2.595 1.00
110_N 208_E 2.588 1.00
13_H 53_T 2.576 1.00
60_E 64_T 2.564 1.00
228_R 241_D 2.438 1.00
281_A 285_K 2.396 1.00
136_S 146_V 2.372 1.00
31_D 34_R 2.372 1.00
54_D 88_K 2.362 1.00
222_L 271_L 2.349 1.00
246_I 271_L 2.257 1.00
285_K 289_A 2.234 1.00
348_H 352_Q 2.227 1.00
132_F 164_A 2.217 1.00
223_R 249_L 2.215 1.00
132_F 162_I 2.214 1.00
232_A 236_H 2.207 1.00
142_R 174_R 2.18 1.00
34_R 37_A 2.167 1.00
27_Q 30_A 2.131 1.00
46_S 50_R 2.046 1.00
236_H 261_R 2.04 1.00
232_A 261_R 2.025 1.00
115_V 128_A 2.017 1.00
61_D 64_T 1.998 1.00
264_H 268_A 1.996 1.00
236_H 240_T 1.987 1.00
236_H 242_S 1.981 1.00
229_S 236_H 1.98 1.00
62_I 82_L 1.959 1.00
66_Q 86_K 1.953 1.00
191_E 195_R 1.944 1.00
57_Q 61_D 1.905 1.00
260_E 265_K 1.894 1.00
91_Q 95_Q 1.881 1.00
332_V 340_L 1.872 1.00
22_L 34_R 1.839 1.00
119_T 200_A 1.838 1.00
283_M 287_Q 1.804 1.00
229_S 239_T 1.792 1.00
138_Y 338_D 1.774 1.00
324_L 339_M 1.757 1.00
247_T 254_V 1.743 1.00
268_A 272_S 1.735 1.00
191_E 197_H 1.728 1.00
287_Q 291_A 1.724 1.00
131_L 201_C 1.703 1.00
146_V 164_A 1.693 1.00
18_E 22_L 1.687 1.00
282_E 286_R 1.659 1.00
88_K 91_Q 1.655 1.00
283_M 286_R 1.65 1.00
273_V 277_R 1.646 1.00
236_H 239_T 1.628 1.00
279_H 283_M 1.605 1.00
132_F 136_S 1.58 1.00
147_E 167_S 1.571 1.00
226_T 241_D 1.536 1.00
110_N 167_S 1.534 1.00
225_D 247_T 1.502 1.00
145_R 167_S 1.498 1.00
200_A 300_S 1.489 1.00
87_E 91_Q 1.474 0.99
331_E 336_K 1.444 0.99
260_E 269_K 1.444 0.99
52_F 56_Q 1.443 0.99
312_F 329_L 1.443 0.99
147_E 165_K 1.428 0.99
42_Y 46_S 1.427 0.99
329_L 333_M 1.413 0.99
17_E 20_Q 1.379 0.99
148_I 162_I 1.369 0.99
132_F 148_I 1.368 0.99
243_A 258_Q 1.355 0.99
102_P 314_Q 1.353 0.99
222_L 249_L 1.322 0.99
226_T 244_I 1.32 0.99
122_D 154_G 1.315 0.99
15_R 18_E 1.31 0.98
56_Q 60_E 1.301 0.98
112_F 165_K 1.296 0.98
224_I 267_K 1.294 0.98
20_Q 46_S 1.293 0.98
151_A 159_Y 1.289 0.98
113_L 166_I 1.286 0.98
58_V 89_S 1.285 0.98
259_D 269_K 1.274 0.98
35_F 39_S 1.272 0.98
84_E 88_K 1.267 0.98
223_R 247_T 1.264 0.98
351_D 354_A 1.261 0.98
28_T 38_L 1.245 0.98
86_K 90_E 1.224 0.97
287_Q 290_E 1.216 0.97
144_W 171_V 1.213 0.97
133_R 334_E 1.212 0.97
264_H 267_K 1.207 0.97
22_L 25_D 1.199 0.97
179_S 204_A 1.194 0.97
253_I 278_I 1.19 0.97
50_R 54_D 1.182 0.97
248_H 251_T 1.182 0.97
265_K 272_S 1.179 0.96
74_M 78_A 1.178 0.96
33_E 36_R 1.175 0.96
58_V 88_K 1.174 0.96
136_S 164_A 1.174 0.96
227_F 245_R 1.164 0.96
248_H 252_G 1.164 0.96
141_A 336_K 1.163 0.96
269_K 272_S 1.154 0.96
98_V 101_L 1.154 0.96
309_T 320_H 1.152 0.96
51_C 95_Q 1.151 0.96
23_L 39_S 1.15 0.96
93_E 97_Q 1.148 0.96
141_A 334_E 1.146 0.96
25_D 28_T 1.134 0.95
300_S 313_P 1.134 0.95
10_E 14_E 1.134 0.95
226_T 232_A 1.13 0.95
261_R 272_S 1.13 0.95
225_D 245_R 1.117 0.95
174_R 338_D 1.117 0.95
23_L 28_T 1.116 0.95
347_E 351_D 1.104 0.94
326_L 329_L 1.102 0.94
326_L 339_M 1.102 0.94
111_A 173_G 1.097 0.94
286_R 290_E 1.092 0.94
33_E 37_A 1.087 0.94
80_D 84_E 1.064 0.93
337_L 341_I 1.06 0.93
236_H 241_D 1.06 0.93
76_E 80_D 1.058 0.93
115_V 203_V 1.055 0.92
285_K 288_Q 1.054 0.92
138_Y 174_R 1.054 0.92
20_Q 42_Y 1.051 0.92
108_E 170_G 1.051 0.92
148_I 151_A 1.049 0.92
47_D 50_R 1.044 0.92
139_A 146_V 1.041 0.92
138_Y 144_W 1.041 0.92
191_E 194_G 1.039 0.92
55_W 59_Q 1.039 0.92
259_D 273_V 1.035 0.91
62_I 89_S 1.027 0.91
115_V 162_I 1.027 0.91
332_V 337_L 1.025 0.91
83_R 86_K 1.02 0.91
126_L 156_H 1.016 0.90
54_D 58_V 1.013 0.90
296_N 302_D 1.013 0.90
226_T 236_H 1.01 0.90
253_I 281_A 1.005 0.90
317_V 340_L 1.004 0.90
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2b3tB111000.098Contact Map0.6
3d5aX10.98331000.106Contact Map0.555
1zbtA10.99171000.115Contact Map0.748
1rq0A30.9251000.171Contact Map0.684
2ihr110.98331000.174Contact Map0.589
1gqeA10.951000.179Contact Map0.635
2rsmA10.31941000.713Contact Map0.5
1j26A10.24171000.8Contact Map0.406
2jvaA10.233399.90.816Contact Map0.347
4dh9Y10.316799.90.816Contact Map0.283
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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