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OPENSEQ.org
RF2 - Peptide chain release factor 2
UniProt: P07012 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10762
Length: 365 (341)
Sequences: 2845
Seq/Len: 8.34
RF2
Paralog alert: 0.83 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: RF1 RF2
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
299_E 302_K 4.552 1.00
130_L 181_I 3.225 1.00
301_Q 305_A 3.164 1.00
159_R 354_D 3.021 1.00
328_L 349_L 2.953 1.00
156_A 183_I 2.846 1.00
297_E 301_Q 2.829 1.00
241_I 263_I 2.776 1.00
244_Y 273_Q 2.755 1.00
37_V 56_L 2.743 1.00
33_R 59_E 2.667 1.00
344_N 347_A 2.591 1.00
72_Q 109_K 2.589 1.00
239_L 263_I 2.579 1.00
31_K 71_D 2.578 1.00
127_D 225_E 2.522 1.00
78_E 82_G 2.508 1.00
245_R 258_E 2.498 1.00
49_E 52_R 2.436 1.00
153_L 163_T 2.434 1.00
298_L 302_K 2.329 1.00
302_K 306_E 2.314 1.00
239_L 288_M 2.269 1.00
149_E 181_I 2.257 1.00
263_I 288_M 2.215 1.00
159_R 191_W 2.212 1.00
149_E 179_V 2.193 1.00
240_R 266_I 2.154 1.00
249_A 253_H 2.107 1.00
45_D 48_N 2.085 1.00
253_H 278_R 2.081 1.00
246_T 253_H 2.077 1.00
84_L 107_E 2.061 1.00
52_R 55_A 2.048 1.00
249_A 278_R 2.029 1.00
253_H 257_T 1.982 1.00
132_I 145_A 1.978 1.00
281_H 285_D 1.965 1.00
208_D 212_R 1.957 1.00
75_Q 79_D 1.948 1.00
253_H 259_S 1.945 1.00
136_S 217_F 1.911 1.00
79_D 82_G 1.904 1.00
64_E 68_D 1.904 1.00
348_V 356_F 1.902 1.00
80_V 103_L 1.865 1.00
40_E 52_R 1.851 1.00
155_W 354_D 1.833 1.00
246_T 256_R 1.825 1.00
300_M 304_N 1.818 1.00
340_V 355_Q 1.816 1.00
277_D 282_K 1.79 1.00
264_T 271_V 1.789 1.00
208_D 214_H 1.775 1.00
304_N 308_Q 1.756 1.00
36_E 40_E 1.737 1.00
299_E 303_K 1.714 1.00
163_T 181_I 1.708 1.00
290_Q 294_K 1.683 1.00
285_D 289_K 1.672 1.00
253_H 256_R 1.65 1.00
149_E 153_L 1.64 1.00
148_L 218_S 1.599 1.00
296_Y 300_M 1.59 1.00
109_K 112_Q 1.579 1.00
347_A 352_S 1.575 1.00
300_M 303_K 1.566 1.00
243_V 258_E 1.544 1.00
164_E 184_S 1.487 1.00
217_F 319_W 1.475 0.99
35_E 38_N 1.474 0.99
242_D 264_T 1.469 0.99
60_R 64_E 1.463 0.99
127_D 184_S 1.459 0.99
107_E 111_A 1.451 0.99
162_K 184_S 1.451 0.99
328_L 345_T 1.447 0.99
277_D 286_Q 1.446 0.99
38_N 64_E 1.443 0.99
70_L 74_K 1.442 0.99
164_E 182_K 1.431 0.99
139_T 171_G 1.426 0.99
329_D 332_R 1.397 0.99
108_E 112_Q 1.376 0.99
345_T 349_L 1.362 0.99
51_E 54_Q 1.345 0.99
239_L 266_I 1.345 0.99
33_R 36_E 1.344 0.99
76_G 110_L 1.334 0.99
53_A 57_G 1.319 0.99
28_D 32_E 1.312 0.99
115_F 119_F 1.312 0.99
129_Y 182_K 1.311 0.99
243_V 261_V 1.303 0.98
168_E 176_I 1.302 0.98
149_E 165_I 1.293 0.98
76_G 109_K 1.293 0.98
260_A 275_Q 1.278 0.98
105_A 109_K 1.274 0.98
270_I 295_L 1.266 0.98
240_R 264_T 1.265 0.98
161_F 188_A 1.256 0.98
150_R 350_D 1.253 0.98
241_I 284_K 1.246 0.98
40_E 43_Q 1.238 0.98
128_C 190_G 1.232 0.98
165_I 179_V 1.232 0.98
46_V 56_L 1.22 0.97
101_A 105_A 1.218 0.97
68_D 72_Q 1.215 0.97
281_H 284_K 1.182 0.97
282_K 289_K 1.181 0.97
158_S 350_D 1.179 0.97
153_L 181_I 1.176 0.97
244_Y 262_R 1.175 0.96
74_K 78_E 1.174 0.96
276_N 286_Q 1.172 0.96
41_L 46_V 1.171 0.96
130_L 183_I 1.17 0.96
265_H 268_T 1.166 0.96
265_H 269_G 1.152 0.96
286_Q 289_K 1.151 0.96
196_T 221_F 1.147 0.96
304_N 307_K 1.145 0.96
158_S 352_S 1.139 0.96
125_S 187_Y 1.133 0.95
278_R 289_K 1.129 0.95
243_V 249_A 1.129 0.95
242_D 262_R 1.125 0.95
43_Q 46_V 1.122 0.95
325_S 336_L 1.118 0.95
38_N 60_R 1.116 0.95
47_W 53_A 1.113 0.95
96_F 100_V 1.112 0.95
303_K 307_K 1.104 0.95
33_R 40_E 1.103 0.94
191_W 354_D 1.097 0.94
101_A 104_D 1.085 0.94
306_E 309_A 1.085 0.94
342_T 345_T 1.085 0.94
41_L 57_G 1.081 0.94
232_I 295_L 1.072 0.93
35_E 39_A 1.069 0.93
80_V 110_L 1.065 0.93
253_H 258_E 1.058 0.93
51_E 55_A 1.057 0.93
72_Q 76_G 1.053 0.93
97_N 101_A 1.048 0.92
348_V 353_L 1.046 0.92
311_E 314_K 1.044 0.92
155_W 161_F 1.041 0.92
302_K 305_A 1.037 0.92
65_A 68_D 1.035 0.92
234_I 288_M 1.035 0.92
129_Y 166_I 1.035 0.92
208_D 211_G 1.033 0.92
73_M 77_L 1.033 0.92
143_D 173_V 1.029 0.91
276_N 290_Q 1.026 0.91
270_I 298_L 1.026 0.91
132_I 179_V 1.022 0.91
353_L 357_I 1.022 0.91
156_A 163_T 1.018 0.91
183_I 192_L 1.017 0.91
86_L 89_E 1.016 0.91
132_I 220_A 1.015 0.91
333_I 356_F 1.014 0.91
284_K 288_M 1.013 0.90
50_P 53_A 1.01 0.90
342_T 355_Q 1.005 0.90
243_V 253_H 1.004 0.90
354_D 358_E 1.002 0.90
34_L 60_R 1 0.90
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1gqeA111000.138Contact Map0.641
2ihr110.96711000.152Contact Map0.57
1zbtA10.97531000.164Contact Map0.737
3d5aX10.92331000.175Contact Map0.559
2b3tB10.9371000.18Contact Map0.591
1rq0A30.87671000.22Contact Map0.682
2rsmA10.31511000.719Contact Map0.486
1j26A10.23841000.804Contact Map0.412
4dh9Y10.31231000.817Contact Map0.287
2jvaA10.230199.90.818Contact Map0.344
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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