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OPENSEQ.org
RUTF - FMN reductase (NADH) RutF
UniProt: P75893 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG13854
Length: 164 (158)
Sequences: 2702
Seq/Len: 17.10
RUTF
Paralog alert: 0.51 [within 20: 0.03] - ratio of genomes with paralogs
Cluster includes: RUTF YDDH
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
120_R 138_E 3.545 1.00
69_L 135_C 3.425 1.00
120_R 136_A 3.304 1.00
122_S 136_A 3.11 1.00
55_R 126_S 2.866 1.00
82_S 156_Y 2.821 1.00
60_W 131_D 2.696 1.00
24_T 70_C 2.687 1.00
101_Q 111_E 2.305 1.00
103_G 118_D 2.193 1.00
93_H 96_A 1.998 1.00
76_A 147_Y 1.9 1.00
70_C 109_Q 1.844 1.00
99_R 111_E 1.757 1.00
33_G 85_F 1.719 1.00
18_A 37_S 1.708 1.00
124_V 131_D 1.702 1.00
83_N 156_Y 1.696 1.00
22_I 85_F 1.661 1.00
14_S 154_R 1.66 1.00
50_L 132_I 1.654 1.00
39_V 49_L 1.61 1.00
52_C 132_I 1.571 1.00
26_D 31_R 1.552 1.00
79_E 83_N 1.552 1.00
107_C 118_D 1.542 1.00
125_V 134_F 1.536 1.00
35_T 82_S 1.528 1.00
105_T 118_D 1.524 1.00
84_L 97_A 1.518 1.00
9_F 13_M 1.516 1.00
64_N 124_V 1.457 1.00
127_V 132_I 1.452 1.00
68_T 120_R 1.435 1.00
48_T 134_F 1.424 1.00
33_G 95_F 1.42 1.00
121_I 133_L 1.418 1.00
16_M 19_A 1.391 0.99
5_D 8_T 1.387 0.99
119_C 135_C 1.368 0.99
49_L 71_V 1.321 0.99
116_S 141_H 1.308 0.99
26_D 29_A 1.307 0.99
31_R 102_T 1.295 0.99
12_A 115_V 1.279 0.99
69_L 119_C 1.278 0.99
42_V 50_L 1.272 0.99
118_D 139_A 1.271 0.99
7_Q 11_D 1.263 0.99
114_L 146_P 1.256 0.99
55_R 131_D 1.248 0.99
60_W 64_N 1.244 0.98
75_S 78_Q 1.214 0.98
48_T 122_S 1.211 0.98
40_C 52_C 1.207 0.98
68_T 107_C 1.206 0.98
21_N 39_V 1.182 0.98
59_V 94_R 1.181 0.98
34_F 59_V 1.181 0.98
54_N 129_T 1.173 0.98
40_C 50_L 1.158 0.97
34_F 53_L 1.15 0.97
103_G 109_Q 1.149 0.97
67_R 121_I 1.139 0.97
72_N 113_A 1.129 0.97
27_G 30_G 1.127 0.97
67_R 124_V 1.126 0.97
22_I 33_G 1.121 0.97
25_T 63_F 1.121 0.97
69_L 121_I 1.099 0.96
72_N 110_L 1.089 0.96
24_T 72_N 1.088 0.96
89_T 92_E 1.088 0.96
42_V 127_V 1.085 0.96
26_D 102_T 1.084 0.96
109_Q 116_S 1.082 0.96
42_V 134_F 1.06 0.95
17_G 150_V 1.057 0.95
12_A 117_F 1.056 0.95
15_C 153_D 1.055 0.95
68_T 138_E 1.048 0.94
44_D 129_T 1.042 0.94
53_L 63_F 1.033 0.94
36_A 51_V 1.027 0.94
59_V 63_F 1.023 0.93
48_T 127_V 1.022 0.93
126_S 131_D 1.005 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3k86A211000.145Contact Map0.828
3cb0A40.98171000.153Contact Map0.853
3pftA20.95731000.154Contact Map0.784
4hx6A80.99391000.155Contact Map0.78
2r0xA10.96341000.158Contact Map0.792
1yoaA10.95731000.16Contact Map0.741
3nfwA40.98781000.163Contact Map0.86
3rh7A60.98171000.164Contact Map0.901
1rz1A80.95121000.175Contact Map0.829
2qckA10.98171000.176Contact Map0.732
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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