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OPENSEQ.org
KEFB - Glutathione-regulated potassium-efflux system protein KefB
UniProt: P45522 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG20110
Length: 601 (581)
Sequences: 1627
Seq/Len: 2.80
KEFB
Paralog alert: 0.60 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: KEFB KEFC YBAL
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
468_I 485_C 4.125 1.00
468_I 492_L 2.819 1.00
310_Y 327_A 2.707 1.00
470_I 482_V 2.579 1.00
405_V 416_G 2.383 1.00
298_V 364_L 2.126 1.00
140_M 145_S 2.116 1.00
485_C 492_L 2.114 1.00
406_V 468_I 2.076 1.00
486_Q 494_I 2.067 1.00
18_V 223_L 2.034 1.00
295_V 299_V 1.971 0.99
258_E 262_A 1.914 0.99
482_V 512_V 1.914 0.99
99_A 121_I 1.889 0.99
302_V 368_T 1.849 0.99
202_R 254_E 1.845 0.99
103_A 120_G 1.816 0.99
486_Q 513_T 1.802 0.99
528_R 532_V 1.788 0.99
287_L 360_V 1.755 0.99
15_F 223_L 1.725 0.98
97_L 101_L 1.668 0.98
122_G 341_F 1.663 0.98
333_G 362_V 1.643 0.97
467_S 530_T 1.641 0.97
196_G 200_L 1.641 0.97
313_A 323_R 1.633 0.97
500_G 503_E 1.609 0.97
42_I 49_F 1.593 0.97
37_L 41_A 1.574 0.97
298_V 365_S 1.572 0.96
402_Q 466_E 1.563 0.96
521_S 549_R 1.556 0.96
332_Q 369_T 1.555 0.96
343_T 346_S 1.539 0.96
153_L 336_F 1.526 0.96
333_G 337_A 1.511 0.95
283_N 356_A 1.508 0.95
288_Y 291_L 1.493 0.95
483_E 510_A 1.492 0.95
106_L 116_A 1.492 0.95
101_L 308_V 1.489 0.95
294_V 357_L 1.486 0.95
300_V 304_V 1.485 0.95
16_A 42_I 1.472 0.94
403_V 419_L 1.46 0.94
103_A 117_V 1.46 0.94
479_M 503_E 1.46 0.94
411_F 526_L 1.45 0.94
301_L 333_G 1.45 0.94
122_G 163_A 1.448 0.94
73_L 248_V 1.438 0.93
403_V 469_V 1.436 0.93
68_M 125_M 1.436 0.93
357_L 361_T 1.431 0.93
470_I 478_T 1.426 0.93
462_A 489_F 1.426 0.93
250_L 259_L 1.419 0.93
167_V 347_Q 1.419 0.93
458_R 463_E 1.415 0.93
498_A 504_A 1.413 0.93
501_R 552_D 1.411 0.93
163_A 340_L 1.41 0.93
512_V 515_F 1.41 0.93
532_V 541_A 1.405 0.92
405_V 415_I 1.402 0.92
301_L 361_T 1.394 0.92
184_G 188_L 1.387 0.92
538_P 542_Q 1.387 0.92
429_L 447_Y 1.376 0.91
104_G 108_L 1.373 0.91
30_G 133_Q 1.367 0.91
99_A 163_A 1.366 0.91
95_V 155_F 1.365 0.91
98_S 156_Q 1.358 0.91
210_A 434_S 1.357 0.91
564_A 586_E 1.356 0.90
405_V 469_V 1.353 0.90
94_Q 336_F 1.352 0.90
470_I 481_L 1.351 0.90
439_M 446_V 1.35 0.90
462_A 488_H 1.341 0.90
310_Y 320_S 1.341 0.90
100_A 104_G 1.338 0.90
474_E 477_D 1.335 0.90
118_V 122_G 1.333 0.89
148_L 318_V 1.332 0.89
282_L 340_L 1.331 0.89
282_L 363_T 1.328 0.89
121_I 163_A 1.327 0.89
126_S 156_Q 1.327 0.89
54_D 57_L 1.317 0.89
130_M 366_M 1.312 0.88
118_V 344_A 1.312 0.88
100_A 371_L 1.305 0.88
201_L 224_L 1.301 0.88
406_V 481_L 1.292 0.87
351_Q 354_Q 1.291 0.87
254_E 440_R 1.285 0.87
521_S 525_E 1.282 0.87
406_V 451_A 1.28 0.87
432_D 435_A 1.279 0.87
57_L 277_S 1.27 0.86
530_T 534_L 1.268 0.86
337_A 362_V 1.264 0.86
517_R 520_F 1.264 0.86
100_A 175_D 1.263 0.86
475_P 478_T 1.253 0.85
100_A 468_I 1.251 0.85
98_S 102_L 1.249 0.85
52_D 55_E 1.234 0.84
111_F 119_G 1.23 0.84
341_F 358_L 1.229 0.84
243_T 323_R 1.22 0.83
74_E 129_A 1.219 0.83
297_S 361_T 1.218 0.83
296_I 300_V 1.216 0.83
156_Q 160_V 1.215 0.83
408_F 416_G 1.214 0.83
208_I 217_F 1.213 0.83
88_F 249_L 1.21 0.82
575_A 583_F 1.206 0.82
129_A 272_G 1.2 0.82
27_L 452_T 1.2 0.82
277_S 430_E 1.199 0.82
193_M 245_I 1.194 0.81
81_W 84_R 1.194 0.81
404_I 461_G 1.192 0.81
416_G 426_I 1.191 0.81
152_V 326_F 1.189 0.81
156_Q 308_V 1.189 0.81
43_G 512_V 1.186 0.81
111_F 116_A 1.186 0.81
260_E 272_G 1.185 0.80
262_A 453_Q 1.181 0.80
529_K 532_V 1.179 0.80
542_Q 546_L 1.178 0.80
314_R 320_S 1.177 0.80
205_F 224_L 1.177 0.80
103_A 113_W 1.174 0.80
571_R 575_A 1.172 0.79
203_P 207_F 1.171 0.79
131_A 193_M 1.171 0.79
482_V 496_A 1.17 0.79
202_R 253_S 1.167 0.79
472_C 481_L 1.167 0.79
409_G 412_G 1.163 0.79
195_I 199_Y 1.161 0.79
566_T 573_R 1.156 0.78
123_L 410_R 1.155 0.78
581_E 584_Q 1.155 0.78
21_V 272_G 1.148 0.77
428_V 444_Y 1.147 0.77
76_N 252_E 1.145 0.77
127_S 332_Q 1.144 0.77
29_I 36_L 1.141 0.77
462_A 468_I 1.141 0.77
474_E 479_M 1.137 0.77
195_I 198_R 1.137 0.77
523_A 530_T 1.131 0.76
583_F 586_E 1.123 0.75
416_G 428_V 1.121 0.75
140_M 373_M 1.121 0.75
88_F 92_A 1.118 0.75
585_R 589_Q 1.118 0.75
289_T 292_L 1.117 0.75
126_S 129_A 1.117 0.75
49_F 436_V 1.117 0.75
229_A 340_L 1.115 0.75
313_A 318_V 1.114 0.74
71_I 229_A 1.108 0.74
46_G 50_I 1.108 0.74
430_E 435_A 1.107 0.74
150_F 363_T 1.105 0.74
32_V 205_F 1.104 0.74
96_L 100_A 1.104 0.74
528_R 541_A 1.102 0.73
12_L 42_I 1.101 0.73
472_C 478_T 1.099 0.73
145_S 325_Q 1.099 0.73
129_A 153_L 1.098 0.73
120_G 124_A 1.098 0.73
126_S 160_V 1.094 0.73
40_I 349_L 1.093 0.72
539_H 550_R 1.093 0.72
149_G 278_V 1.09 0.72
156_Q 336_F 1.088 0.72
536_M 540_Q 1.086 0.72
7_L 10_G 1.083 0.71
408_F 428_V 1.083 0.71
575_A 584_Q 1.082 0.71
65_V 68_M 1.081 0.71
328_G 372_L 1.079 0.71
100_A 103_A 1.078 0.71
483_E 486_Q 1.077 0.71
435_A 500_G 1.077 0.71
204_V 345_S 1.076 0.71
578_E 581_E 1.076 0.71
119_G 358_L 1.075 0.71
145_S 322_E 1.073 0.70
289_T 353_D 1.072 0.70
538_P 560_I 1.07 0.70
124_A 156_Q 1.07 0.70
401_P 466_E 1.069 0.70
93_A 131_A 1.069 0.70
539_H 546_L 1.068 0.70
345_S 355_M 1.068 0.70
23_L 466_E 1.067 0.70
422_N 540_Q 1.067 0.70
221_T 276_I 1.067 0.70
405_V 499_R 1.066 0.70
568_Q 571_R 1.066 0.70
403_V 440_R 1.065 0.70
94_Q 156_Q 1.064 0.70
131_A 153_L 1.064 0.70
543_R 546_L 1.064 0.70
571_R 577_R 1.063 0.69
418_L 527_G 1.062 0.69
461_G 464_A 1.06 0.69
514_Q 553_M 1.058 0.69
485_C 489_F 1.057 0.69
410_R 499_R 1.056 0.69
581_E 585_R 1.056 0.69
71_I 128_T 1.054 0.68
469_V 530_T 1.051 0.68
131_A 330_L 1.049 0.68
13_F 30_G 1.049 0.68
36_L 64_V 1.049 0.68
462_A 492_L 1.048 0.68
138_K 205_F 1.048 0.68
10_G 286_V 1.047 0.68
161_I 240_A 1.046 0.68
63_G 67_L 1.046 0.68
288_Y 307_L 1.045 0.68
221_T 259_L 1.043 0.67
518_E 522_S 1.042 0.67
410_R 500_G 1.042 0.67
302_V 372_L 1.041 0.67
83_L 448_Y 1.039 0.67
33_L 367_M 1.039 0.67
404_I 462_A 1.038 0.67
274_F 279_G 1.032 0.66
230_L 234_A 1.032 0.66
259_L 264_D 1.031 0.66
572_A 576_R 1.031 0.66
581_E 586_E 1.03 0.66
573_R 576_R 1.029 0.66
578_E 583_F 1.028 0.66
469_V 526_L 1.028 0.66
100_A 303_A 1.026 0.65
331_S 368_T 1.026 0.65
125_M 369_T 1.025 0.65
249_L 257_H 1.024 0.65
539_H 545_Q 1.024 0.65
16_A 49_F 1.023 0.65
310_Y 314_R 1.022 0.65
566_T 589_Q 1.022 0.65
41_A 97_L 1.021 0.65
537_H 540_Q 1.019 0.65
129_A 160_V 1.019 0.65
553_M 582_I 1.018 0.65
581_E 589_Q 1.018 0.65
44_P 449_G 1.017 0.64
73_L 250_L 1.016 0.64
22_P 219_A 1.016 0.64
376_V 380_L 1.015 0.64
149_G 329_V 1.013 0.64
153_L 156_Q 1.013 0.64
517_R 523_A 1.012 0.64
64_V 338_F 1.011 0.64
495_L 533_T 1.011 0.64
94_Q 153_L 1.011 0.64
70_I 130_M 1.01 0.64
556_L 559_L 1.009 0.64
150_F 156_Q 1.008 0.63
226_L 258_E 1.006 0.63
343_T 347_Q 1.005 0.63
219_A 268_G 1.005 0.63
500_G 518_E 1.004 0.63
565_D 572_A 1.003 0.63
20_A 41_A 1.002 0.63
451_A 481_L 1.002 0.63
289_T 311_L 1.001 0.63
19_A 42_I 1.001 0.63
493_H 533_T 1.001 0.63
578_E 584_Q 1 0.63
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4bwzA10.64731000.642Contact Map0.389
3l9wA20.34111000.772Contact Map0.728
1zcdA20.582499.90.842Contact Map0.174
4gx0A40.522599.90.844Contact Map0.718
1lnqA80.499299.90.846Contact Map0.71
3c85A40.297899.90.852Contact Map0.61
2aefA20.334499.90.852Contact Map0.756
3nafA10.394399.90.859Contact Map0.583
1id1A20.247999.90.86Contact Map0.718
3mt5A10.321199.90.861Contact Map0.605
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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