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OPENSEQ.org
MPL - UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase
UniProt: P37773 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12440
Length: 457 (444)
Sequences: 4153
Seq/Len: 9.35
MPL
Paralog alert: 0.97 [within 20: 0.36] - ratio of genomes with paralogs
Cluster includes: MPL MURC MURD MURE MURF
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
21_R 47_I 4.074 1.00
80_A 83_E 3.962 1.00
80_A 84_K 3.662 1.00
124_I 287_A 3.134 1.00
125_L 156_V 3.068 1.00
317_L 325_T 3.062 1.00
182_I 290_A 3.032 1.00
251_K 260_E 3.019 1.00
108_V 121_A 2.96 1.00
106_L 121_A 2.946 1.00
121_A 182_I 2.853 1.00
180_T 290_A 2.668 1.00
320_E 325_T 2.583 1.00
120_M 275_L 2.545 1.00
357_V 387_L 2.415 1.00
272_K 306_G 2.408 1.00
320_E 323_G 2.386 1.00
18_M 45_Q 2.346 1.00
107_A 178_P 2.335 1.00
201_Q 228_Q 2.293 1.00
288_I 301_A 2.283 1.00
2_R 26_E 2.253 1.00
383_E 423_T 2.229 1.00
412_D 415_T 2.225 1.00
115_T 311_A 2.211 1.00
397_A 401_E 2.121 1.00
107_A 159_A 2.102 1.00
122_T 139_V 2.095 1.00
123_W 307_S 2.087 1.00
424_A 430_I 2.074 1.00
419_M 422_K 2.042 1.00
121_A 287_A 2.029 1.00
317_L 327_Y 2.017 1.00
78_V 82_L 2.011 1.00
127_Q 304_A 1.995 1.00
317_L 320_E 1.981 1.00
353_R 428_D 1.969 1.00
187_E 335_T 1.962 1.00
195_D 199_A 1.961 1.00
241_V 286_M 1.917 1.00
326_V 421_V 1.913 1.00
121_A 290_A 1.904 1.00
108_V 118_A 1.901 1.00
127_Q 307_S 1.899 1.00
284_G 305_L 1.894 1.00
127_Q 303_N 1.853 1.00
280_N 283_N 1.848 1.00
112_H 333_H 1.833 1.00
183_L 208_V 1.827 1.00
196_D 199_A 1.826 1.00
297_A 300_D 1.82 1.00
121_A 291_A 1.818 1.00
124_I 291_A 1.81 1.00
249_A 285_L 1.804 1.00
291_A 301_A 1.797 1.00
292_R 298_P 1.796 1.00
263_L 292_R 1.788 1.00
315_L 344_L 1.788 1.00
372_D 402_A 1.775 1.00
381_A 384_V 1.773 1.00
408_H 419_M 1.773 1.00
383_E 408_H 1.765 1.00
40_T 44_K 1.762 1.00
410_S 419_M 1.742 1.00
182_I 286_M 1.735 1.00
355_I 385_F 1.702 1.00
87_P 97_D 1.701 1.00
35_Y 43_E 1.7 1.00
385_F 423_T 1.696 1.00
111_T 189_D 1.696 1.00
165_A 203_Q 1.685 1.00
181_L 207_L 1.677 1.00
79_E 82_L 1.66 1.00
181_L 211_V 1.658 1.00
324_V 424_A 1.656 1.00
106_L 294_V 1.653 1.00
208_V 217_I 1.619 1.00
188_F 191_A 1.598 1.00
104_W 179_R 1.595 1.00
4_H 62_P 1.582 1.00
220_P 241_V 1.579 1.00
437_G 440_G 1.577 1.00
356_A 381_A 1.546 1.00
249_A 281_M 1.538 1.00
131_K 153_D 1.538 1.00
259_W 284_G 1.537 1.00
327_Y 344_L 1.535 1.00
40_T 43_E 1.522 1.00
206_H 209_R 1.517 1.00
247_W 289_A 1.514 1.00
216_R 293_H 1.51 1.00
385_F 410_S 1.477 1.00
303_N 307_S 1.467 1.00
123_W 126_E 1.462 1.00
221_E 245_G 1.459 1.00
197_L 225_N 1.453 1.00
65_V 81_V 1.45 1.00
419_M 423_T 1.448 1.00
79_E 83_E 1.436 1.00
202_K 206_H 1.43 0.99
125_L 294_V 1.429 0.99
165_A 193_I 1.423 0.99
361_R 389_P 1.418 0.99
92_P 159_A 1.412 0.99
300_D 303_N 1.4 0.99
216_R 239_E 1.399 0.99
74_G 79_E 1.393 0.99
93_Q 175_H 1.386 0.99
20_A 94_W 1.383 0.99
124_I 128_C 1.379 0.99
186_L 201_Q 1.374 0.99
355_I 423_T 1.369 0.99
179_R 215_G 1.368 0.99
249_A 252_L 1.368 0.99
250_K 262_L 1.365 0.99
26_E 48_E 1.357 0.99
260_E 270_E 1.355 0.99
5_I 20_A 1.342 0.99
8_I 29_G 1.339 0.99
319_G 446_L 1.338 0.99
154_F 294_V 1.334 0.99
125_L 296_V 1.334 0.99
194_F 203_Q 1.333 0.99
198_K 202_K 1.332 0.99
128_C 296_V 1.326 0.99
353_R 383_E 1.322 0.99
418_D 422_K 1.319 0.99
6_L 71_M 1.317 0.99
39_S 43_E 1.312 0.99
279_H 283_N 1.304 0.99
197_L 201_Q 1.302 0.99
115_T 138_G 1.298 0.99
211_V 215_G 1.291 0.99
323_G 427_G 1.29 0.99
73_R 82_L 1.285 0.99
216_R 237_E 1.285 0.99
3_I 64_L 1.282 0.99
128_C 300_D 1.28 0.99
219_W 240_L 1.27 0.99
107_A 207_L 1.27 0.99
180_T 293_H 1.268 0.98
262_L 267_K 1.254 0.98
338_L 342_A 1.25 0.98
395_Q 398_E 1.243 0.98
198_K 201_Q 1.242 0.98
4_H 28_T 1.24 0.98
220_P 282_H 1.236 0.98
356_A 433_M 1.23 0.98
397_A 409_W 1.225 0.98
124_I 301_A 1.217 0.98
411_G 415_T 1.209 0.98
183_L 186_L 1.207 0.98
278_E 342_A 1.2 0.98
201_Q 225_N 1.199 0.98
385_F 419_M 1.193 0.98
2_R 63_D 1.188 0.97
180_T 294_V 1.18 0.97
162_Y 362_S 1.177 0.97
184_N 286_M 1.173 0.97
382_D 425_Q 1.163 0.97
108_V 182_I 1.149 0.97
11_T 14_G 1.146 0.97
396_V 409_W 1.141 0.97
442_H 446_L 1.138 0.96
260_E 267_K 1.137 0.96
382_D 428_D 1.136 0.96
222_N 282_H 1.132 0.96
355_I 424_A 1.128 0.96
177_C 210_I 1.127 0.96
432_V 442_H 1.123 0.96
353_R 382_D 1.122 0.96
221_E 240_L 1.121 0.96
386_L 400_A 1.12 0.96
124_I 304_A 1.12 0.96
275_L 284_G 1.117 0.96
183_L 217_I 1.111 0.96
225_N 228_Q 1.108 0.96
18_M 41_L 1.101 0.95
160_D 163_D 1.1 0.95
16_L 94_W 1.094 0.95
259_W 271_V 1.094 0.95
164_C 193_I 1.087 0.95
415_T 418_D 1.085 0.95
291_A 296_V 1.084 0.95
221_E 227_K 1.083 0.95
67_I 78_V 1.079 0.95
202_K 232_M 1.079 0.95
106_L 180_T 1.073 0.95
111_T 187_E 1.073 0.95
443_Q 447_D 1.069 0.94
258_E 270_E 1.069 0.94
353_R 425_Q 1.068 0.94
342_A 380_R 1.065 0.94
250_K 260_E 1.063 0.94
119_G 123_W 1.062 0.94
93_Q 97_D 1.062 0.94
59_E 84_K 1.058 0.94
258_E 272_K 1.057 0.94
120_M 284_G 1.056 0.94
385_F 420_V 1.055 0.94
189_D 333_H 1.054 0.94
414_D 444_K 1.051 0.94
387_L 410_S 1.049 0.94
211_V 217_I 1.049 0.94
322_N 421_V 1.049 0.94
358_L 386_L 1.048 0.94
27_V 42_L 1.043 0.93
312_R 316_E 1.042 0.93
64_L 89_M 1.037 0.93
425_Q 428_D 1.034 0.93
78_V 88_Y 1.034 0.93
318_R 443_Q 1.027 0.93
104_W 153_D 1.026 0.93
290_A 294_V 1.026 0.93
199_A 202_K 1.025 0.93
164_C 203_Q 1.025 0.93
408_H 423_T 1.023 0.93
420_V 424_A 1.023 0.93
253_T 270_E 1.023 0.93
447_D 450_A 1.021 0.92
387_L 438_F 1.014 0.92
131_K 151_E 1.011 0.92
206_H 210_I 1.01 0.92
106_L 290_A 1.008 0.92
296_V 300_D 1.003 0.92
4_H 30_S 1.001 0.92
104_W 154_F 1.001 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3hn7A10.99561000.185Contact Map0.753
4hv4A20.97161000.231Contact Map0.765
1p3dA20.95841000.233Contact Map0.777
1j6uA10.94531000.245Contact Map0.755
3lk7A10.92121000.289Contact Map0.757
2x5oA10.91681000.299Contact Map0.699
4bucA20.89931000.311Contact Map0.682
3eagA20.68271000.413Contact Map0.796
3zm6A10.91471000.471Contact Map0.674
1gg4A20.93221000.471Contact Map0.657
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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