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LAST - Uncharacterized tRNA/rRNA methyltransferase LasT
UniProt: P37005 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12309
Length: 228 (226)
Sequences: 896
Seq/Len: 3.96
LAST
Paralog alert: 0.37 [within 20: 0.00] - ratio of genomes with paralogs
Cluster includes: LAST TRMJ
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
63_T 66_E 3.984 1.00
24_K 53_D 3.484 1.00
65_A 126_L 3.252 1.00
30_D 59_K 3.141 1.00
18_A 47_V 2.884 1.00
71_V 109_A 2.824 1.00
7_L 144_L 2.679 1.00
131_T 151_Y 2.656 1.00
201_Q 204_L 2.654 1.00
9_A 35_D 2.536 1.00
32_R 61_F 2.469 1.00
9_A 114_R 2.421 1.00
5_I 152_C 2.363 1.00
92_V 157_T 2.36 1.00
62_P 66_E 2.357 1.00
64_L 111_V 2.318 1.00
114_R 123_E 2.275 1.00
44_T 55_I 2.141 1.00
76_A 127_A 2.137 1.00
201_Q 206_L 2.089 1.00
76_A 124_L 2.072 1.00
45_R 56_D 2.056 1.00
180_R 216_H 2.048 1.00
158_L 204_L 2.014 1.00
144_L 148_V 1.997 1.00
4_T 32_R 1.997 1.00
35_D 62_P 1.97 1.00
158_L 201_Q 1.843 1.00
32_R 59_K 1.827 1.00
22_A 149_M 1.79 0.99
112_F 148_V 1.766 0.99
6_I 109_A 1.76 0.99
5_I 23_M 1.757 0.99
110_L 151_Y 1.7 0.99
74_T 111_V 1.657 0.99
23_M 28_F 1.624 0.99
30_D 107_H 1.544 0.98
120_T 123_E 1.51 0.98
201_Q 205_G 1.491 0.97
173_Q 176_A 1.49 0.97
75_V 94_L 1.481 0.97
7_L 148_V 1.478 0.97
64_L 68_L 1.442 0.97
170_D 173_Q 1.44 0.97
92_V 154_Q 1.434 0.96
184_L 223_E 1.424 0.96
199_W 204_L 1.394 0.96
114_R 120_T 1.392 0.96
68_L 74_T 1.389 0.96
173_Q 209_Q 1.388 0.96
185_L 218_L 1.386 0.96
47_V 146_Q 1.365 0.95
79_A 140_P 1.336 0.94
74_T 171_Q 1.326 0.94
8_V 119_L 1.31 0.93
73_F 129_V 1.309 0.93
35_D 63_T 1.295 0.93
26_M 149_M 1.291 0.93
4_T 30_D 1.288 0.93
187_T 223_E 1.276 0.92
183_T 223_E 1.274 0.92
176_A 216_H 1.268 0.92
14_E 43_A 1.261 0.92
188_L 221_D 1.257 0.91
200_L 203_R 1.241 0.91
112_F 144_L 1.224 0.90
197_V 203_R 1.209 0.89
196_L 203_R 1.206 0.89
33_I 36_S 1.199 0.89
14_E 47_V 1.196 0.88
188_L 222_I 1.183 0.88
203_R 206_L 1.181 0.88
4_T 61_F 1.179 0.88
184_L 222_I 1.168 0.87
70_D 172_H 1.14 0.85
199_W 203_R 1.136 0.85
169_A 212_T 1.122 0.84
212_T 216_H 1.118 0.83
78_T 130_L 1.115 0.83
184_L 219_L 1.109 0.83
32_R 70_D 1.087 0.81
70_D 107_H 1.087 0.81
72_D 106_S 1.087 0.81
8_V 114_R 1.087 0.81
34_V 67_S 1.071 0.80
111_V 127_A 1.07 0.80
158_L 206_L 1.066 0.79
68_L 71_V 1.062 0.79
79_A 82_R 1.055 0.78
117_S 120_T 1.051 0.78
18_A 25_T 1.049 0.78
97_L 129_V 1.044 0.77
24_K 51_S 1.033 0.76
82_R 152_C 1.029 0.76
6_I 71_V 1.029 0.76
38_A 41_E 1.024 0.75
28_F 136_V 1.019 0.75
28_F 152_C 1.01 0.74
128_D 174_L 1.008 0.74
220_H 224_K 1.007 0.74
55_I 58_I 1.007 0.74
177_L 181_A 1.005 0.73
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3onpA10.99561000.313Contact Map0.784
3ilkA20.97371000.325Contact Map0.794
3ic6A10.8071000.502Contact Map0.752
3ktyA30.69741000.552Contact Map0.765
3dcmX10.68861000.604Contact Map0.628
1zjrA10.80261000.616Contact Map0.53
1v2xA10.74121000.619Contact Map0.603
1x7oA20.67111000.637Contact Map0.573
3n4jA10.66671000.644Contact Map0.653
2ha8A20.67541000.645Contact Map0.461
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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