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OPENSEQ.org
PRPC - 2-methylcitrate synthase
UniProt: P31660 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11756
Length: 389 (370)
Sequences: 2000
Seq/Len: 5.41
PRPC
Paralog alert: 0.54 [within 20: 0.03] - ratio of genomes with paralogs
Cluster includes: CISY PRPC
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
212_I 225_G 5.837 1.00
51_D 381_P 4.387 1.00
73_R 77_A 3.813 1.00
209_S 226_A 3.736 1.00
48_D 51_D 3.256 1.00
69_K 75_E 2.973 1.00
256_E 309_R 2.877 1.00
56_C 382_F 2.839 1.00
171_G 187_E 2.808 1.00
168_S 171_G 2.618 1.00
262_R 267_E 2.616 1.00
183_S 297_E 2.614 1.00
254_E 257_A 2.595 1.00
309_R 312_T 2.576 1.00
292_K 308_D 2.543 1.00
54_K 289_R 2.527 1.00
73_R 386_D 2.467 1.00
215_T 229_A 2.446 1.00
384_A 387_K 2.342 1.00
95_T 98_E 2.289 1.00
57_E 76_L 2.232 1.00
72_T 75_E 2.227 1.00
186_W 335_M 2.204 1.00
69_K 380_R 2.191 1.00
211_V 368_I 2.17 1.00
168_S 187_E 2.117 1.00
23_S 366_K 2.113 1.00
204_A 215_T 2.101 1.00
208_T 229_A 2.046 1.00
247_Q 333_N 2.024 1.00
212_I 229_A 1.981 1.00
77_A 81_T 1.955 1.00
261_K 265_N 1.944 1.00
177_L 337_V 1.931 1.00
113_G 146_I 1.907 1.00
240_E 244_E 1.899 1.00
250_E 254_E 1.872 1.00
289_R 293_Q 1.797 1.00
172_H 176_L 1.777 1.00
217_S 231_R 1.777 1.00
74_D 77_A 1.702 1.00
114_V 227_I 1.698 1.00
309_R 313_V 1.688 1.00
185_S 297_E 1.682 1.00
288_K 292_K 1.668 1.00
337_V 345_L 1.664 1.00
385_L 388_R 1.655 0.99
167_D 171_G 1.635 0.99
147_L 348_I 1.62 0.99
63_H 69_K 1.581 0.99
74_D 78_A 1.575 0.99
82_K 137_D 1.568 0.99
83_L 137_D 1.568 0.99
128_T 131_G 1.564 0.99
59_E 83_L 1.56 0.99
91_A 95_T 1.557 0.99
147_L 344_P 1.547 0.99
47_Y 380_R 1.545 0.99
257_A 261_K 1.532 0.99
136_A 355_A 1.515 0.99
188_K 192_I 1.5 0.99
193_S 332_Y 1.494 0.99
307_A 330_V 1.492 0.99
51_D 55_H 1.489 0.99
152_H 157_G 1.484 0.99
164_T 175_H 1.462 0.98
111_R 228_G 1.451 0.98
292_K 296_Q 1.448 0.98
112_T 115_S 1.441 0.98
160_I 179_G 1.438 0.98
77_A 80_K 1.431 0.98
188_K 293_Q 1.427 0.98
170_G 191_H 1.424 0.98
383_V 388_R 1.416 0.98
95_T 99_A 1.403 0.98
78_A 82_K 1.403 0.98
188_K 191_H 1.403 0.98
204_A 229_A 1.399 0.98
66_I 140_L 1.397 0.98
143_L 351_V 1.393 0.98
47_Y 70_L 1.392 0.98
62_A 352_T 1.359 0.97
60_E 76_L 1.356 0.97
260_R 264_E 1.355 0.97
261_K 267_E 1.353 0.97
384_A 388_R 1.347 0.97
61_V 195_V 1.34 0.97
145_S 162_P 1.338 0.97
94_R 149_Y 1.328 0.97
260_R 317_S 1.324 0.97
50_L 285_Q 1.317 0.96
289_R 292_K 1.317 0.96
261_K 264_E 1.301 0.96
129_V 132_A 1.294 0.96
111_R 115_S 1.291 0.96
89_L 149_Y 1.28 0.96
349_A 352_T 1.28 0.96
36_G 39_G 1.271 0.95
292_K 304_Y 1.267 0.95
193_S 287_I 1.264 0.95
250_E 302_K 1.252 0.95
164_T 181_K 1.235 0.94
44_Y 198_A 1.234 0.94
96_V 99_A 1.232 0.94
29_N 375_V 1.221 0.94
256_E 316_E 1.217 0.94
37_K 40_N 1.214 0.94
383_V 386_D 1.214 0.94
225_G 229_A 1.213 0.94
183_S 186_W 1.212 0.94
105_H 240_E 1.208 0.93
291_A 330_V 1.208 0.93
301_L 333_N 1.202 0.93
96_V 116_A 1.197 0.93
107_M 239_N 1.183 0.93
114_V 143_L 1.181 0.92
110_M 147_L 1.181 0.92
64_L 70_L 1.174 0.92
72_T 385_L 1.171 0.92
148_L 173_F 1.162 0.92
48_D 381_P 1.16 0.92
192_I 290_V 1.158 0.91
252_P 312_T 1.153 0.91
115_S 228_G 1.135 0.90
251_T 302_K 1.127 0.90
258_D 262_R 1.123 0.90
140_L 352_T 1.123 0.90
90_P 101_P 1.121 0.90
242_S 306_I 1.121 0.90
109_V 150_W 1.121 0.90
33_C 199_E 1.121 0.90
167_D 187_E 1.119 0.90
252_P 309_R 1.111 0.89
198_A 352_T 1.104 0.89
23_S 275_P 1.098 0.88
56_C 195_V 1.098 0.88
259_I 313_V 1.094 0.88
86_L 138_K 1.093 0.88
154_S 340_E 1.091 0.88
213_A 358_I 1.087 0.88
81_T 85_A 1.087 0.88
75_E 78_A 1.086 0.88
308_D 312_T 1.082 0.87
184_Q 188_K 1.08 0.87
235_H 238_A 1.075 0.87
171_G 184_Q 1.074 0.87
60_E 382_F 1.073 0.87
87_R 165_D 1.067 0.86
87_R 169_I 1.064 0.86
167_D 172_H 1.063 0.86
52_L 61_V 1.062 0.86
280_A 285_Q 1.057 0.86
201_E 371_S 1.054 0.85
256_E 260_R 1.051 0.85
262_R 318_K 1.044 0.85
94_R 98_E 1.044 0.85
252_P 306_I 1.041 0.84
288_K 308_D 1.036 0.84
208_T 225_G 1.033 0.84
100_L 119_C 1.033 0.84
208_T 211_V 1.028 0.83
50_L 286_V 1.024 0.83
93_V 113_G 1.024 0.83
82_K 134_D 1.022 0.83
208_T 212_I 1.022 0.83
213_A 222_A 1.022 0.83
254_E 261_K 1.019 0.83
221_S 231_R 1.016 0.82
197_Y 324_L 1.008 0.82
111_R 224_I 1.006 0.82
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3o8jA1011000.074Contact Map0.75
2h12A60.98971000.087Contact Map0.762
2ibpA20.99231000.098Contact Map0.704
1iomA10.95891000.099Contact Map0.616
1o7xA40.95371000.104Contact Map0.744
3tqgA20.96141000.104Contact Map0.724
1vgmA20.95371000.105Contact Map0.714
3hwkA80.98461000.107Contact Map0.742
1vgpA10.9461000.107Contact Map0.639
2ifcA40.96661000.108Contact Map0.779
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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