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RFAH - Transcription antitermination protein RfaH
UniProt: P0AFW0 Sequence
Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10839
Length: 162 (161)
Sequences: 1652
Seq/Len: 10.26

RFAH
Paralog alert: 0.29 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: NUSG RFAH
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
18_Q 22_E 4.511 1.00
90_S 94_H 4.027 1.00
27_N 58_E 3.001 1.00
117_I 160_R 2.948 1.00
139_S 159_F 2.903 1.00
34_T 45_A 2.844 1.00
132_E 140_M 2.456 1.00
34_T 47_S 2.334 1.00
114_D 161_K 2.11 1.00
33_I 48_E 1.941 1.00
138_R 155_K 1.931 1.00
17_A 75_V 1.851 1.00
37_K 48_E 1.849 1.00
120_E 158_E 1.779 1.00
59_F 65_H 1.73 1.00
115_K 127_Q 1.729 1.00
28_C 136_E 1.687 1.00
138_R 153_S 1.68 1.00
50_L 56_F 1.678 1.00
21_L 28_C 1.662 1.00
36_E 43_R 1.623 1.00
154_V 158_E 1.602 1.00
32_M 47_S 1.553 1.00
128_A 141_L 1.503 1.00
111_Y 114_D 1.46 1.00
131_T 142_L 1.432 1.00
117_I 127_Q 1.42 1.00
17_A 55_L 1.416 1.00
59_F 69_I 1.404 0.99
39_V 44_T 1.401 0.99
21_L 69_I 1.375 0.99
36_E 45_A 1.345 0.99
13_Q 74_G 1.326 0.99
26_V 58_E 1.316 0.99
10_K 13_Q 1.314 0.99
89_P 92_V 1.291 0.99
51_F 96_L 1.288 0.99
112_P 133_P 1.278 0.99
6_L 88_V 1.255 0.99
62_E 65_H 1.248 0.99
21_L 29_L 1.221 0.98
8_Y 76_S 1.218 0.98
35_L 48_E 1.206 0.98
29_L 97_S 1.206 0.98
91_A 94_H 1.204 0.98
58_E 90_S 1.199 0.98
141_L 154_V 1.198 0.98
51_F 56_F 1.184 0.98
139_S 156_N 1.18 0.98
117_I 125_G 1.172 0.98
93_I 97_S 1.171 0.98
65_H 69_I 1.17 0.97
9_C 14_L 1.162 0.97
21_L 136_E 1.161 0.97
143_L 152_H 1.152 0.97
155_K 158_E 1.147 0.97
116_V 141_L 1.143 0.97
22_E 28_C 1.138 0.97
141_L 151_K 1.119 0.96
8_Y 77_H 1.118 0.96
16_R 20_H 1.116 0.96
25_A 28_C 1.112 0.96
142_L 149_E 1.089 0.96
16_R 23_R 1.076 0.95
80_R 87_I 1.074 0.95
144_N 149_E 1.067 0.95
17_A 72_T 1.056 0.95
38_I 43_R 1.051 0.94
85_P 123_F 1.042 0.94
94_H 99_Y 1.024 0.93
66_T 70_N 1.023 0.93
19_E 23_R 1.02 0.93
88_V 93_I 1.019 0.93
154_V 159_F 1.009 0.93
40_R 44_T 1.004 0.93
115_K 129_I 1.002 0.92
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2ougA40.99381000.081Contact Map0.539
2jvvA111000.109Contact Map0.804
2lq8A10.98771000.144Contact Map0.54
1m1hA111000.144Contact Map0.629
2xhcA10.96911000.261Contact Map0.477
3p8bB20.87651000.304Contact Map0.764
1nz8A10.64299.90.416Contact Map0.457
3lpeA40.512399.70.589Contact Map0.587
2e6zA10.3642990.713Contact Map0.665
1nz9A10.3519990.716Contact Map0.775

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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