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OPENSEQ.org
PBP2 - Penicillin-binding protein 2
UniProt: P0AD65 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10606
Length: 633 (602)
Sequences: 3467
Seq/Len: 5.76
PBP2
Paralog alert: 0.79 [within 20: 0.07] - ratio of genomes with paralogs
Cluster includes: FTSI PBP2
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
273_V 607_I 4.261 1.00
542_A 580_Y 3.751 1.00
543_A 574_M 3.727 1.00
520_Y 540_K 3.485 1.00
279_R 582_N 3.476 1.00
282_G 485_M 3.422 1.00
262_I 273_V 3.097 1.00
369_D 388_A 3.052 1.00
38_N 41_N 3.045 1.00
277_D 584_Q 3.022 1.00
350_T 398_D 2.888 1.00
214_Y 420_D 2.844 1.00
282_G 482_H 2.83 1.00
230_N 234_R 2.816 1.00
74_R 282_G 2.778 1.00
354_D 388_A 2.732 1.00
575_T 588_A 2.712 1.00
383_S 392_F 2.692 1.00
275_V 587_V 2.684 1.00
342_S 511_Y 2.647 1.00
214_Y 250_I 2.633 1.00
378_L 382_R 2.574 1.00
383_S 391_F 2.562 1.00
347_T 350_T 2.545 1.00
139_T 142_Q 2.503 1.00
473_I 542_A 2.487 1.00
263_E 287_V 2.454 1.00
396_A 452_G 2.444 1.00
72_Y 249_D 2.432 1.00
354_D 369_D 2.418 1.00
88_I 138_L 2.405 1.00
278_P 586_A 2.399 1.00
276_T 466_S 2.374 1.00
375_H 388_A 2.362 1.00
66_P 222_T 2.36 1.00
469_L 519_M 2.311 1.00
471_I 478_V 2.309 1.00
576_A 608_L 2.305 1.00
469_L 575_T 2.282 1.00
379_N 382_R 2.274 1.00
169_G 293_D 2.252 1.00
351_T 377_R 2.225 1.00
215_E 219_H 2.222 1.00
516_K 577_F 2.214 1.00
265_L 606_Q 2.202 1.00
343_A 407_W 2.202 1.00
418_G 424_E 2.176 1.00
406_E 410_K 2.173 1.00
589_M 604_M 2.143 1.00
467_K 478_V 2.141 1.00
70_I 249_D 2.135 1.00
276_T 481_P 2.125 1.00
277_D 282_G 2.107 1.00
381_T 521_G 2.08 1.00
585_V 611_I 2.079 1.00
180_S 207_K 2.079 1.00
540_K 581_N 2.069 1.00
40_Y 44_I 2.045 1.00
275_V 585_V 2.045 1.00
74_R 255_D 2.004 1.00
520_Y 580_Y 2.002 1.00
262_I 607_I 1.973 1.00
267_A 289_T 1.971 1.00
269_S 593_N 1.964 1.00
417_T 424_E 1.953 1.00
210_I 252_L 1.952 1.00
279_R 584_Q 1.947 1.00
343_A 403_R 1.945 1.00
175_V 254_L 1.938 1.00
338_V 411_F 1.93 1.00
475_D 516_K 1.916 1.00
332_V 408_M 1.91 1.00
345_V 403_R 1.902 1.00
420_D 482_H 1.893 1.00
72_Y 251_Y 1.877 1.00
261_Y 610_H 1.867 1.00
103_D 113_D 1.863 1.00
576_A 604_M 1.859 1.00
480_V 500_H 1.857 1.00
402_D 432_R 1.83 1.00
343_A 399_M 1.819 1.00
334_P 472_L 1.816 1.00
470_M 586_A 1.812 1.00
77_I 257_K 1.808 1.00
261_Y 265_L 1.802 1.00
369_D 375_H 1.793 1.00
455_Q 549_Q 1.793 1.00
37_A 41_N 1.787 1.00
382_R 386_E 1.787 1.00
380_V 391_F 1.78 1.00
337_A 392_F 1.765 1.00
466_S 588_A 1.743 1.00
591_L 603_L 1.728 1.00
277_D 280_T 1.725 1.00
276_T 283_V 1.723 1.00
508_H 511_Y 1.719 1.00
262_I 285_A 1.719 1.00
385_E 521_G 1.717 1.00
274_V 466_S 1.715 1.00
74_R 284_L 1.713 1.00
173_T 291_S 1.698 1.00
335_Y 468_A 1.692 1.00
604_M 608_L 1.684 1.00
413_Y 468_A 1.682 1.00
260_Q 264_T 1.675 1.00
378_L 386_E 1.668 1.00
394_Q 398_D 1.662 1.00
112_T 115_D 1.659 1.00
605_R 609_D 1.643 1.00
258_L 275_V 1.629 0.99
168_Y 256_L 1.625 0.99
138_L 142_Q 1.623 0.99
210_I 250_I 1.622 0.99
258_L 284_L 1.619 0.99
339_S 407_W 1.615 0.99
284_L 585_V 1.604 0.99
46_R 49_D 1.604 0.99
276_T 470_M 1.598 0.99
58_R 228_E 1.598 0.99
382_R 385_E 1.598 0.99
223_G 244_P 1.597 0.99
543_A 601_G 1.581 0.99
359_Q 366_R 1.574 0.99
550_V 592_E 1.568 0.99
579_P 583_P 1.568 0.99
78_P 81_L 1.565 0.99
251_Y 485_M 1.565 0.99
357_W 366_R 1.549 0.99
91_M 128_R 1.548 0.99
262_I 271_A 1.541 0.99
342_S 508_H 1.536 0.99
520_Y 524_N 1.532 0.99
172_L 254_L 1.531 0.99
536_S 605_R 1.531 0.99
38_N 42_L 1.529 0.99
578_A 612_M 1.522 0.99
340_A 395_V 1.52 0.99
223_G 247_G 1.505 0.99
351_T 379_N 1.499 0.99
253_T 282_G 1.497 0.99
513_E 516_K 1.495 0.99
80_A 167_P 1.492 0.99
385_E 527_N 1.49 0.99
403_R 406_E 1.49 0.99
338_V 511_Y 1.487 0.99
186_D 207_K 1.485 0.99
272_A 321_A 1.476 0.99
253_T 482_H 1.467 0.99
172_L 263_E 1.454 0.99
385_E 525_R 1.451 0.99
414_G 427_G 1.44 0.98
334_P 519_M 1.437 0.98
573_L 590_I 1.437 0.98
477_I 503_P 1.424 0.98
466_S 586_A 1.418 0.98
88_I 156_V 1.413 0.98
87_Q 161_Y 1.407 0.98
578_A 608_L 1.402 0.98
135_K 142_Q 1.402 0.98
266_L 287_V 1.402 0.98
523_A 543_A 1.401 0.98
49_D 53_R 1.399 0.98
278_P 470_M 1.39 0.98
522_V 530_A 1.384 0.98
346_I 380_V 1.381 0.98
413_Y 460_A 1.378 0.98
302_S 305_D 1.367 0.98
70_I 81_L 1.36 0.98
606_Q 609_D 1.358 0.98
353_F 377_R 1.356 0.97
210_I 483_L 1.348 0.97
371_K 374_G 1.335 0.97
356_G 376_G 1.333 0.97
393_Y 453_I 1.332 0.97
75_N 255_D 1.326 0.97
530_A 545_S 1.321 0.97
181_K 202_T 1.319 0.97
266_L 271_A 1.318 0.97
217_V 248_H 1.315 0.97
307_S 311_N 1.314 0.97
275_V 611_I 1.313 0.97
335_Y 413_Y 1.311 0.97
384_L 519_M 1.307 0.97
338_V 515_A 1.306 0.97
221_Q 245_Q 1.304 0.97
597_G 601_G 1.298 0.97
400_G 403_R 1.285 0.96
473_I 577_F 1.278 0.96
465_M 573_L 1.277 0.96
251_Y 486_S 1.277 0.96
303_S 307_S 1.276 0.96
41_N 46_R 1.273 0.96
276_T 467_K 1.272 0.96
381_T 518_G 1.272 0.96
273_V 589_M 1.269 0.96
384_L 518_G 1.269 0.96
283_V 419_I 1.265 0.96
509_S 513_E 1.265 0.96
210_I 214_Y 1.261 0.96
262_I 265_L 1.257 0.96
475_D 512_W 1.25 0.95
450_P 456_G 1.247 0.95
550_V 593_N 1.246 0.95
40_Y 45_V 1.235 0.95
479_K 499_P 1.22 0.95
249_D 488_A 1.219 0.95
263_E 291_S 1.206 0.94
381_T 517_D 1.192 0.94
149_N 152_R 1.191 0.94
286_L 321_A 1.191 0.94
251_Y 488_A 1.19 0.94
355_P 390_T 1.188 0.93
72_Y 78_P 1.184 0.93
414_G 464_Q 1.179 0.93
360_L 449_I 1.175 0.93
409_G 415_H 1.173 0.93
419_I 483_L 1.173 0.93
33_G 37_A 1.167 0.93
97_N 100_Q 1.166 0.93
90_M 156_V 1.165 0.93
421_L 483_L 1.163 0.93
250_I 484_L 1.163 0.93
523_A 574_M 1.162 0.92
88_I 158_V 1.16 0.92
70_I 78_P 1.16 0.92
66_P 223_G 1.159 0.92
587_V 604_M 1.153 0.92
173_T 292_Y 1.149 0.92
318_V 324_G 1.147 0.92
104_A 107_S 1.143 0.92
62_V 225_E 1.138 0.91
258_L 611_I 1.138 0.91
474_N 479_K 1.135 0.91
91_M 94_K 1.135 0.91
129_F 133_P 1.134 0.91
220_G 223_G 1.134 0.91
370_W 374_G 1.131 0.91
331_T 465_M 1.13 0.91
345_V 399_M 1.129 0.91
543_A 576_A 1.126 0.91
87_Q 133_P 1.125 0.91
346_I 350_T 1.123 0.91
475_D 513_E 1.114 0.90
214_Y 217_V 1.113 0.90
447_D 450_P 1.109 0.90
358_W 393_Y 1.104 0.90
405_S 409_G 1.103 0.90
462_P 588_A 1.103 0.90
334_P 469_L 1.102 0.90
272_A 288_S 1.101 0.90
146_F 153_F 1.096 0.89
402_D 406_E 1.095 0.89
70_I 82_N 1.087 0.89
305_D 308_A 1.078 0.88
589_M 603_L 1.078 0.88
77_I 256_L 1.077 0.88
322_T 423_E 1.073 0.88
334_P 575_T 1.067 0.88
400_G 448_T 1.066 0.87
408_M 429_M 1.066 0.87
572_K 597_G 1.062 0.87
327_P 571_H 1.061 0.87
214_Y 484_L 1.061 0.87
545_S 576_A 1.057 0.87
467_K 471_I 1.055 0.87
453_I 549_Q 1.053 0.87
425_R 461_T 1.051 0.86
385_E 518_G 1.05 0.86
170_S 291_S 1.049 0.86
375_H 386_E 1.049 0.86
73_D 255_D 1.048 0.86
79_L 172_L 1.046 0.86
542_A 577_F 1.044 0.86
66_P 247_G 1.043 0.86
577_F 580_Y 1.042 0.86
460_A 465_M 1.04 0.86
401_I 448_T 1.04 0.86
378_L 388_A 1.037 0.85
599_A 602_T 1.037 0.85
591_L 599_A 1.034 0.85
226_E 240_K 1.032 0.85
170_S 263_E 1.03 0.85
345_V 398_D 1.029 0.85
359_Q 362_G 1.027 0.85
337_A 380_V 1.022 0.84
279_R 475_D 1.018 0.84
358_W 397_Y 1.018 0.84
325_V 459_T 1.016 0.84
578_A 585_V 1.016 0.84
275_V 607_I 1.014 0.84
89_E 157_E 1.013 0.84
67_S 246_A 1.009 0.83
208_L 292_Y 1.006 0.83
322_T 421_L 1.005 0.83
346_I 395_V 1.002 0.83
473_I 586_A 1.001 0.83
113_D 117_A 1 0.83
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2wadA30.89261000.26Contact Map0.746
3equA20.82781000.285Contact Map0.794
3vskA20.84681000.285Contact Map0.73
1vqqA20.91151000.31Contact Map0.735
3ue3A10.8121000.317Contact Map0.743
1qmeA10.85621000.318Contact Map0.728
3pbtA10.8121000.322Contact Map0.767
4jbfA20.73931000.345Contact Map0.744
4mnrA10.69351000.388Contact Map0.734
3un7A20.69831000.44Contact Map0.719
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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