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OPENSEQ.org
MBHS - Hydrogenase-1 small chain
UniProt: P69739 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10468
Length: 372 (352)
Sequences: 382
Seq/Len: 1.09
MBHS
Paralog alert: 0.64 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: MBHS MBHT
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
271_T 296_G 2.978 1.00
113_N 150_G 2.644 1.00
334_V 338_A 2.605 1.00
247_V 264_M 2.545 0.99
273_N 298_A 2.512 0.99
58_H 61_E 2.45 0.99
103_E 107_D 2.31 0.99
115_K 152_S 2.293 0.99
94_M 101_A 2.132 0.98
60_L 123_N 2.047 0.97
116_Y 150_G 2.038 0.97
285_S 289_Q 2.002 0.96
126_L 177_T 1.959 0.96
105_F 147_A 1.941 0.95
29_A 42_I 1.923 0.95
138_R 146_R 1.892 0.94
157_W 201_M 1.873 0.94
59_G 140_F 1.853 0.93
61_E 167_Q 1.807 0.92
163_W 221_G 1.772 0.91
250_W 264_M 1.771 0.91
159_T 163_W 1.747 0.91
128_E 132_F 1.733 0.90
58_H 65_C 1.718 0.90
155_I 205_I 1.689 0.88
20_S 23_K 1.687 0.88
196_P 250_W 1.624 0.86
279_R 289_Q 1.599 0.85
163_W 300_N 1.588 0.84
64_C 165_C 1.582 0.84
138_R 142_E 1.58 0.84
118_L 147_A 1.576 0.83
128_E 131_M 1.575 0.83
324_A 328_G 1.565 0.83
132_F 135_S 1.551 0.82
330_T 334_V 1.545 0.82
149_A 186_K 1.53 0.81
127_G 175_Q 1.529 0.81
61_E 196_P 1.516 0.80
197_I 274_A 1.492 0.78
58_H 63_T 1.482 0.78
61_E 65_C 1.48 0.78
147_A 154_I 1.459 0.76
66_T 90_D 1.451 0.76
18_R 21_F 1.443 0.75
119_A 123_N 1.404 0.72
126_L 141_I 1.399 0.72
197_I 200_V 1.396 0.72
200_V 274_A 1.39 0.71
107_D 111_Q 1.387 0.71
152_S 208_M 1.376 0.70
65_C 196_P 1.372 0.70
337_A 341_V 1.368 0.69
58_H 196_P 1.357 0.69
154_I 188_I 1.35 0.68
116_Y 149_A 1.34 0.67
156_A 179_I 1.324 0.66
336_A 344_V 1.318 0.65
192_P 263_K 1.314 0.65
253_D 257_K 1.311 0.65
191_V 196_P 1.308 0.64
319_G 324_A 1.305 0.64
141_I 145_K 1.299 0.64
197_I 251_D 1.297 0.63
248_Q 263_K 1.292 0.63
109_I 147_A 1.279 0.62
135_S 143_K 1.27 0.61
118_L 148_A 1.263 0.60
203_A 256_R 1.256 0.60
54_V 205_I 1.252 0.59
231_I 276_S 1.239 0.58
238_R 242_D 1.239 0.58
220_M 300_N 1.237 0.58
56_W 218_D 1.236 0.58
58_H 167_Q 1.235 0.58
53_P 112_Y 1.234 0.58
330_T 341_V 1.233 0.58
132_F 136_S 1.231 0.57
250_W 293_G 1.227 0.57
29_A 35_G 1.221 0.57
63_T 196_P 1.217 0.56
109_I 150_G 1.204 0.55
152_S 212_D 1.197 0.54
333_G 337_A 1.195 0.54
161_A 178_P 1.195 0.54
312_V 315_I 1.183 0.53
133_C 140_F 1.18 0.53
133_C 136_S 1.179 0.53
93_L 264_M 1.177 0.52
155_I 201_M 1.175 0.52
136_S 189_I 1.174 0.52
182_V 186_K 1.169 0.52
326_T 330_T 1.169 0.52
60_L 140_F 1.168 0.52
115_K 212_D 1.165 0.51
236_Y 274_A 1.161 0.51
207_Y 215_P 1.16 0.51
187_P 207_Y 1.156 0.50
159_T 264_M 1.153 0.50
226_F 274_A 1.15 0.50
274_A 278_T 1.15 0.50
340_G 343_A 1.149 0.50
22_L 278_T 1.148 0.50
162_S 227_Y 1.147 0.50
261_L 291_G 1.143 0.49
240_H 245_E 1.142 0.49
200_V 280_W 1.14 0.49
61_E 250_W 1.138 0.49
340_G 344_V 1.137 0.49
274_A 280_W 1.137 0.49
80_V 288_I 1.135 0.48
5_E 13_R 1.135 0.48
159_T 300_N 1.134 0.48
55_V 108_I 1.133 0.48
117_I 227_Y 1.132 0.48
66_T 97_A 1.129 0.48
64_C 157_W 1.128 0.48
126_L 130_G 1.124 0.47
58_H 121_E 1.116 0.47
236_Y 288_I 1.114 0.46
108_I 151_A 1.112 0.46
99_T 138_R 1.11 0.46
311_R 342_H 1.105 0.46
332_L 335_V 1.105 0.46
173_P 287_P 1.104 0.45
328_G 342_H 1.104 0.45
207_Y 213_R 1.097 0.45
189_I 214_L 1.093 0.44
71_R 303_W 1.091 0.44
94_M 105_F 1.088 0.44
308_F 312_V 1.081 0.43
11_M 17_T 1.078 0.43
273_N 303_W 1.075 0.43
124_P 179_I 1.073 0.43
331_A 338_A 1.069 0.42
325_D 342_H 1.068 0.42
183_I 187_P 1.067 0.42
307_S 313_V 1.065 0.42
13_R 135_S 1.065 0.42
208_M 212_D 1.063 0.42
33_G 65_C 1.06 0.41
190_K 300_N 1.059 0.41
163_W 190_K 1.058 0.41
343_A 350_Q 1.057 0.41
45_A 92_T 1.055 0.41
231_I 271_T 1.055 0.41
109_I 146_R 1.054 0.41
18_R 23_K 1.053 0.41
153_A 187_P 1.052 0.41
110_T 113_N 1.051 0.41
318_M 343_A 1.048 0.40
126_L 182_V 1.048 0.40
182_V 241_F 1.047 0.40
24_Y 46_L 1.047 0.40
161_A 179_I 1.046 0.40
159_T 192_P 1.042 0.40
156_A 163_W 1.041 0.40
182_V 185_D 1.041 0.40
130_G 141_I 1.037 0.39
59_G 287_P 1.036 0.39
216_D 295_L 1.036 0.39
97_A 303_W 1.035 0.39
237_R 261_L 1.034 0.39
323_T 331_A 1.033 0.39
207_Y 212_D 1.033 0.39
216_D 224_L 1.032 0.39
200_V 226_F 1.032 0.39
188_I 205_I 1.029 0.39
68_S 177_T 1.028 0.38
57_I 191_V 1.026 0.38
197_I 280_W 1.026 0.38
200_V 236_Y 1.025 0.38
67_E 71_R 1.024 0.38
119_A 201_M 1.023 0.38
348_V 351_R 1.022 0.38
88_D 104_V 1.021 0.38
82_L 133_C 1.019 0.38
120_V 140_F 1.016 0.37
46_L 210_T 1.015 0.37
174_T 323_T 1.015 0.37
105_F 139_P 1.013 0.37
106_E 146_R 1.011 0.37
327_V 330_T 1.008 0.37
274_A 286_F 1.005 0.36
68_S 280_W 1.005 0.36
336_A 347_A 1.001 0.36
323_T 333_G 1.001 0.36
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3uqyS20.8791000.303Contact Map0.515
1h2aS10.83061000.304Contact Map0.521
3rgwS10.8791000.311Contact Map0.527
2wpnA10.78491000.338Contact Map0.493
3ayxB20.75271000.358Contact Map0.55
1wuiS10.70971000.383Contact Map0.54
3myrA40.71771000.383Contact Map0.523
1yqwA30.7071000.397Contact Map0.543
1yq9A20.70161000.397Contact Map0.563
4kn9S20.70161000.397Contact Map0.537
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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