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OPENSEQ.org
GSPE - Putative type II secretion system protein E
UniProt: P45759 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12891
Length: 493 (481)
Sequences: 2052
Seq/Len: 4.27
GSPE
Paralog alert: 0.56 [within 20: 0.02] - ratio of genomes with paralogs
Cluster includes: GSPE HOFB
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
381_L 412_P 5.371 1.00
436_T 459_Q 4.952 1.00
206_R 272_N 3.959 1.00
234_L 262_I 3.893 1.00
237_L 371_G 3.822 1.00
387_Q 413_V 3.706 1.00
347_S 350_G 3.535 1.00
386_R 410_G 3.352 1.00
381_L 477_S 3.322 1.00
442_A 455_L 3.294 1.00
271_R 314_V 3.165 1.00
161_I 207_A 3.09 1.00
235_E 265_A 2.941 1.00
387_Q 411_T 2.921 1.00
384_Q 417_H 2.919 1.00
373_I 432_M 2.794 1.00
388_F 408_T 2.689 1.00
274_L 307_I 2.604 1.00
276_V 303_G 2.564 1.00
467_G 481_V 2.558 1.00
320_I 329_A 2.539 1.00
457_R 465_K 2.523 1.00
11_W 15_Q 2.503 1.00
112_I 160_L 2.452 1.00
442_A 447_V 2.45 1.00
389_T 411_T 2.449 1.00
190_R 213_D 2.433 1.00
439_L 452_L 2.423 1.00
206_R 288_G 2.395 1.00
248_T 343_L 2.364 1.00
392_S 395_Q 2.354 1.00
380_R 427_M 2.31 1.00
246_L 341_S 2.305 1.00
435_V 443_I 2.29 1.00
53_L 58_F 2.279 1.00
177_I 296_V 2.276 1.00
466_N 470_K 2.2 1.00
436_T 455_L 2.18 1.00
199_L 290_T 2.148 1.00
238_I 262_I 2.134 1.00
14_A 66_F 2.132 1.00
347_S 453_E 2.13 1.00
202_I 280_V 2.109 1.00
389_T 409_I 2.106 1.00
198_T 207_A 2.09 1.00
229_A 461_K 2.085 1.00
231_K 235_E 2.026 1.00
412_P 477_S 2.025 1.00
170_L 198_T 2.019 1.00
455_L 459_Q 2.009 1.00
448_D 451_A 2.008 1.00
168_A 196_V 1.991 1.00
40_I 48_Q 1.98 1.00
455_L 458_Q 1.975 1.00
349_S 433_M 1.971 1.00
381_L 386_R 1.964 1.00
136_M 157_A 1.933 1.00
194_V 209_L 1.902 1.00
148_T 151_Q 1.877 1.00
427_M 475_D 1.877 1.00
236_N 434_V 1.877 1.00
438_E 459_Q 1.875 1.00
212_L 282_Y 1.866 1.00
409_I 471_V 1.843 1.00
123_T 424_Q 1.825 1.00
145_V 483_R 1.81 1.00
464_I 485_A 1.8 1.00
230_D 430_H 1.751 0.99
468_L 472_I 1.748 0.99
197_S 310_Q 1.742 0.99
290_T 307_I 1.737 0.99
266_L 314_V 1.728 0.99
230_D 432_M 1.724 0.99
293_N 296_V 1.721 0.99
352_V 369_L 1.716 0.99
276_V 307_I 1.664 0.99
469_Q 473_S 1.659 0.99
167_M 183_I 1.656 0.99
230_D 466_N 1.656 0.99
145_V 379_R 1.656 0.99
461_K 469_Q 1.651 0.99
206_R 274_L 1.643 0.99
194_V 211_L 1.635 0.99
240_L 370_A 1.629 0.99
246_L 320_I 1.61 0.99
381_L 413_V 1.606 0.99
470_K 475_D 1.604 0.99
415_C 418_C 1.593 0.99
34_F 38_L 1.575 0.99
137_S 148_T 1.57 0.99
227_T 230_D 1.566 0.99
228_A 232_Q 1.536 0.98
327_Q 331_Q 1.499 0.98
299_S 302_R 1.487 0.98
267_N 273_I 1.486 0.98
129_I 136_M 1.482 0.98
201_S 288_G 1.482 0.98
326_A 360_V 1.468 0.97
349_S 452_L 1.466 0.97
29_L 53_L 1.465 0.97
135_T 151_Q 1.46 0.97
25_E 49_T 1.455 0.97
245_I 338_L 1.449 0.97
266_L 340_L 1.447 0.97
356_R 365_L 1.446 0.97
471_V 481_V 1.437 0.97
445_E 451_A 1.424 0.97
382_C 385_C 1.421 0.97
226_M 430_H 1.394 0.96
230_D 233_D 1.385 0.96
456_V 460_H 1.36 0.96
438_E 458_Q 1.359 0.96
385_C 418_C 1.358 0.95
303_G 307_I 1.351 0.95
391_V 409_I 1.349 0.95
226_M 234_L 1.339 0.95
331_Q 357_D 1.333 0.95
382_C 418_C 1.329 0.95
237_L 432_M 1.327 0.95
22_S 46_Q 1.316 0.94
437_P 440_R 1.316 0.94
334_L 364_L 1.304 0.94
438_E 455_L 1.297 0.94
183_I 194_V 1.292 0.94
14_A 62_L 1.291 0.94
382_C 415_C 1.286 0.93
320_I 341_S 1.273 0.93
197_S 208_V 1.272 0.93
374_A 463_L 1.272 0.93
385_C 415_C 1.267 0.93
457_R 462_A 1.265 0.93
232_Q 236_N 1.262 0.93
170_L 207_A 1.259 0.93
447_V 451_A 1.256 0.92
130_E 139_R 1.256 0.92
384_Q 424_Q 1.255 0.92
185_L 194_V 1.251 0.92
168_A 183_I 1.238 0.92
411_T 479_D 1.233 0.91
389_T 395_Q 1.229 0.91
192_I 213_D 1.228 0.91
13_L 17_I 1.227 0.91
381_L 410_G 1.218 0.91
120_I 187_I 1.214 0.91
301_A 324_E 1.202 0.90
441_A 445_E 1.2 0.90
15_Q 63_E 1.199 0.90
476_T 480_E 1.198 0.90
54_S 57_D 1.195 0.90
423_Y 477_S 1.192 0.90
248_T 345_T 1.178 0.89
230_D 373_I 1.172 0.88
349_S 453_E 1.165 0.88
171_D 295_R 1.156 0.88
9_A 14_A 1.154 0.87
143_D 379_R 1.151 0.87
331_Q 334_L 1.148 0.87
276_V 290_T 1.146 0.87
233_D 434_V 1.143 0.87
462_A 465_K 1.14 0.87
356_R 446_N 1.137 0.86
304_L 328_I 1.137 0.86
454_R 458_Q 1.135 0.86
226_M 428_A 1.134 0.86
40_I 44_V 1.133 0.86
142_I 147_R 1.12 0.85
378_V 476_T 1.118 0.85
18_G 59_E 1.108 0.84
36_R 40_I 1.105 0.84
26_I 44_V 1.102 0.84
272_N 288_G 1.085 0.83
423_Y 476_T 1.085 0.83
112_I 164_I 1.084 0.83
123_T 142_I 1.077 0.82
20_L 61_R 1.077 0.82
245_I 371_G 1.075 0.82
165_K 205_E 1.073 0.82
451_A 454_R 1.072 0.82
238_I 340_L 1.072 0.82
214_K 282_Y 1.067 0.81
224_L 430_H 1.064 0.81
227_T 469_Q 1.059 0.81
37_L 50_M 1.057 0.80
388_F 410_G 1.054 0.80
124_A 142_I 1.04 0.79
30_A 58_F 1.039 0.79
206_R 310_Q 1.038 0.79
330_V 360_V 1.036 0.78
414_G 418_C 1.029 0.78
259_L 316_M 1.028 0.78
372_I 433_M 1.028 0.78
227_T 466_N 1.026 0.78
391_V 395_Q 1.022 0.77
164_I 196_V 1.02 0.77
130_E 310_Q 1.019 0.77
192_I 211_L 1.016 0.77
274_L 290_T 1.014 0.76
27_I 51_T 1.014 0.76
468_L 478_W 1.012 0.76
227_T 470_K 1.01 0.76
482_M 485_A 1.01 0.76
267_N 286_G 1.006 0.76
193_D 214_K 1.004 0.75
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1p9rA10.84581000.394Contact Map0.745
tr|GY00.83161000.396Contact Map
2ewvA10.68971000.606Contact Map0.593
3jvvA30.66731000.61Contact Map0.573
2oap120.88441000.674Contact Map0.329
2eyuA20.47261000.717Contact Map0.77
4ihqA30.90671000.719Contact Map0.314
4bloA120.58011000.719Contact Map0.086
2b8tA40.40371000.801Contact Map0.435
2gzaA30.62881000.819Contact Map0.571
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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