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OPENSEQ.org
GARD - D-galactarate dehydratase
UniProt: P39829 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG12522
Length: 523 (502)
Sequences: 544
Seq/Len: 1.08
GARD
Paralog alert: 0.46 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: GARD UXAA
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
495_D 500_K 4.577 1.00
124_R 281_E 4.031 1.00
121_E 276_T 3.729 1.00
279_A 494_L 3.376 1.00
318_M 431_I 3.127 1.00
338_N 341_V 2.973 1.00
307_S 317_V 2.965 1.00
124_R 277_C 2.939 1.00
76_A 80_I 2.868 1.00
480_E 488_K 2.671 1.00
415_T 429_S 2.653 1.00
59_D 82_R 2.65 1.00
33_L 45_L 2.578 1.00
470_I 489_L 2.535 0.99
281_E 408_R 2.527 0.99
339_E 343_K 2.467 0.99
458_T 462_N 2.436 0.99
303_V 437_T 2.37 0.99
123_Y 131_G 2.365 0.99
45_L 55_V 2.352 0.99
124_R 275_E 2.349 0.99
66_I 91_V 2.34 0.99
471_N 477_T 2.285 0.99
59_D 81_P 2.257 0.98
47_E 84_S 2.254 0.98
471_N 474_T 2.251 0.98
62_A 79_A 2.249 0.98
56_A 80_I 2.151 0.98
343_K 347_E 2.146 0.98
446_L 451_V 2.064 0.97
461_A 468_M 1.985 0.96
166_N 270_N 1.957 0.95
205_G 269_L 1.95 0.95
61_P 64_G 1.928 0.95
60_I 80_I 1.897 0.94
152_V 258_I 1.879 0.94
433_V 493_I 1.875 0.94
25_A 57_L 1.836 0.93
199_S 223_L 1.823 0.93
277_C 281_E 1.792 0.92
491_H 495_D 1.764 0.91
302_A 489_L 1.736 0.90
61_P 79_A 1.705 0.89
455_A 461_A 1.682 0.88
60_I 64_G 1.669 0.88
316_T 411_I 1.661 0.87
354_N 358_M 1.656 0.87
149_V 211_I 1.624 0.86
60_I 76_A 1.615 0.85
396_V 407_K 1.589 0.84
118_Y 494_L 1.588 0.84
485_V 488_K 1.588 0.84
118_Y 491_H 1.588 0.84
246_E 249_V 1.558 0.82
480_E 485_V 1.553 0.82
427_V 446_L 1.538 0.81
156_I 259_L 1.529 0.81
459_E 463_R 1.51 0.79
130_V 277_C 1.504 0.79
257_D 260_Q 1.497 0.79
344_R 397_E 1.496 0.78
137_G 204_F 1.494 0.78
455_A 468_M 1.489 0.78
431_I 448_A 1.478 0.77
71_E 331_L 1.472 0.77
337_V 396_V 1.455 0.76
284_V 306_A 1.449 0.75
387_I 422_C 1.435 0.74
127_D 500_K 1.428 0.74
33_L 55_V 1.425 0.74
121_E 500_K 1.422 0.73
26_I 68_R 1.414 0.73
206_G 265_H 1.414 0.73
152_V 255_V 1.406 0.72
284_V 433_V 1.406 0.72
180_G 217_K 1.393 0.71
192_I 390_S 1.384 0.70
136_L 269_L 1.375 0.70
475_I 482_I 1.374 0.70
515_A 522_V 1.373 0.70
81_P 84_S 1.369 0.69
287_Q 426_Q 1.369 0.69
302_A 475_I 1.366 0.69
58_L 64_G 1.366 0.69
80_I 86_I 1.365 0.69
91_V 377_A 1.365 0.69
420_F 444_Y 1.36 0.69
435_V 454_M 1.358 0.68
284_V 493_I 1.351 0.68
474_T 488_K 1.347 0.67
474_T 480_E 1.339 0.67
481_T 484_E 1.337 0.67
23_N 60_I 1.328 0.66
309_L 486_G 1.328 0.66
118_Y 279_A 1.326 0.66
62_A 78_R 1.325 0.66
452_I 496_V 1.32 0.65
492_F 500_K 1.318 0.65
350_E 353_D 1.306 0.64
119_T 278_P 1.303 0.64
504_F 507_Q 1.301 0.64
302_A 486_G 1.301 0.64
124_R 408_R 1.285 0.62
34_K 48_H 1.284 0.62
461_A 471_N 1.27 0.61
23_N 58_L 1.256 0.59
241_V 264_R 1.256 0.59
133_K 506_D 1.253 0.59
336_A 345_L 1.247 0.59
458_T 471_N 1.246 0.59
363_R 523_T 1.245 0.58
87_D 377_A 1.242 0.58
486_G 489_L 1.24 0.58
126_A 236_E 1.239 0.58
140_T 149_V 1.236 0.58
474_T 485_V 1.233 0.57
328_A 387_I 1.232 0.57
470_I 492_F 1.22 0.56
305_Y 482_I 1.218 0.56
456_T 475_I 1.217 0.56
454_M 472_A 1.215 0.56
345_L 412_Y 1.211 0.55
480_E 484_E 1.199 0.54
33_L 61_P 1.194 0.54
320_S 426_Q 1.194 0.54
306_A 310_L 1.19 0.53
285_G 434_Q 1.188 0.53
136_L 262_A 1.187 0.53
246_E 251_F 1.186 0.53
458_T 473_G 1.18 0.52
427_V 449_V 1.174 0.52
318_M 405_P 1.166 0.51
309_L 487_W 1.166 0.51
245_D 249_V 1.161 0.51
38_R 43_L 1.158 0.50
66_I 76_A 1.157 0.50
278_P 462_N 1.156 0.50
147_G 150_D 1.148 0.49
310_L 315_A 1.146 0.49
458_T 483_E 1.144 0.49
104_L 342_G 1.139 0.49
59_D 79_A 1.136 0.48
305_Y 483_E 1.136 0.48
344_R 471_N 1.133 0.48
148_V 255_V 1.128 0.47
326_R 353_D 1.126 0.47
337_V 341_V 1.126 0.47
180_G 369_P 1.125 0.47
447_M 511_H 1.123 0.47
212_G 384_L 1.119 0.47
492_F 496_V 1.116 0.46
286_M 456_T 1.114 0.46
284_V 456_T 1.108 0.46
132_T 432_T 1.108 0.46
268_K 271_Q 1.107 0.45
376_L 470_I 1.105 0.45
15_Y 471_N 1.104 0.45
17_K 25_A 1.102 0.45
424_T 514_L 1.099 0.45
75_Y 94_P 1.098 0.45
379_V 386_S 1.092 0.44
35_A 48_H 1.091 0.44
356_L 464_W 1.086 0.44
309_L 490_F 1.085 0.43
397_E 407_K 1.081 0.43
492_F 495_D 1.077 0.43
374_G 440_R 1.076 0.43
316_T 415_T 1.075 0.43
243_L 246_E 1.075 0.43
156_I 266_L 1.075 0.43
286_M 303_V 1.074 0.42
15_Y 25_A 1.071 0.42
62_A 408_R 1.071 0.42
177_Y 517_F 1.07 0.42
328_A 367_P 1.067 0.42
365_A 378_N 1.066 0.42
27_I 33_L 1.066 0.42
282_L 497_A 1.064 0.41
212_G 381_E 1.064 0.41
20_D 60_I 1.064 0.41
23_N 67_I 1.062 0.41
268_K 272_R 1.062 0.41
456_T 493_I 1.06 0.41
459_E 462_N 1.057 0.41
201_N 496_V 1.055 0.41
46_I 81_P 1.053 0.40
322_V 386_S 1.052 0.40
361_T 523_T 1.052 0.40
275_E 281_E 1.051 0.40
514_L 517_F 1.048 0.40
16_I 200_L 1.047 0.40
148_V 381_E 1.046 0.40
138_I 260_Q 1.046 0.40
270_N 273_Q 1.045 0.40
278_P 281_E 1.045 0.40
287_Q 423_G 1.043 0.40
443_P 510_L 1.043 0.40
367_P 378_N 1.042 0.39
102_L 346_L 1.041 0.39
333_T 346_L 1.039 0.39
433_V 463_R 1.038 0.39
187_A 521_P 1.038 0.39
328_A 384_L 1.037 0.39
49_I 275_E 1.037 0.39
266_L 270_N 1.037 0.39
136_L 172_G 1.033 0.39
452_I 492_F 1.031 0.38
157_E 169_G 1.025 0.38
217_K 523_T 1.025 0.38
120_F 497_A 1.023 0.38
198_I 448_A 1.021 0.38
341_V 397_E 1.021 0.38
144_C 179_C 1.02 0.38
45_L 49_I 1.017 0.37
88_E 381_E 1.017 0.37
293_A 423_G 1.016 0.37
170_V 458_T 1.013 0.37
336_A 341_V 1.01 0.37
129_S 283_V 1.008 0.36
17_K 41_D 1.006 0.36
120_F 130_V 1.005 0.36
491_H 500_K 1.005 0.36
123_Y 272_R 1.004 0.36
129_S 281_E 1.003 0.36
306_A 489_L 1.002 0.36
192_I 218_L 1.001 0.36
153_V 381_E 1 0.36
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
3lazA20.17971000.856Contact Map0.83
3k3sA80.17021000.868Contact Map0.899
3teeA10.267778.80.98Contact Map0.296
1ucsA10.118577.80.98Contact Map0.561
1opsA10.1185770.98Contact Map0.381
1msiA10.1372.30.981Contact Map0.588
1wvoA10.135864.50.982Contact Map0.298
1c89A10.170258.10.983Contact Map0.19
3g8rA20.196952.20.983Contact Map0.226
2wqpA10.175950.10.984Contact Map0.242
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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