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OPENSEQ.org
TREC - Trehalose-6-phosphate hydrolase
UniProt: P28904 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11402
Length: 551 (546)
Sequences: 5687
Seq/Len: 10.42
TREC
Paralog alert: 0.84 [within 20: 0.23] - ratio of genomes with paralogs
Cluster includes: AMY2 GLGX MALZ SUCP TREC
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
40_D 93_R 5.992 1.00
22_Q 38_H 5.105 1.00
89_Q 92_S 4.785 1.00
84_D 192_R 4.588 1.00
106_T 127_Y 4.294 1.00
91_K 195_D 4.267 1.00
33_R 85_E 4.154 1.00
131_D 158_Q 4.059 1.00
83_F 87_V 3.755 1.00
349_M 545_A 3.754 1.00
42_L 47_V 3.705 1.00
89_Q 93_R 3.514 1.00
179_R 235_E 3.444 1.00
44_K 460_Q 3.397 1.00
57_V 72_A 3.245 1.00
90_A 97_I 3.214 1.00
322_C 364_E 3.148 1.00
90_A 95_I 3.133 1.00
33_R 82_D 3.094 1.00
349_M 458_T 3.034 1.00
79_T 82_D 3.024 1.00
33_R 36_I 2.973 1.00
353_G 547_W 2.891 1.00
353_G 490_Y 2.874 1.00
80_L 192_R 2.812 1.00
43_H 93_R 2.797 1.00
50_I 90_A 2.717 1.00
367_G 455_V 2.706 1.00
366_I 445_V 2.585 1.00
16_I 35_V 2.553 1.00
348_A 459_Y 2.552 1.00
22_Q 37_Q 2.444 1.00
57_V 68_A 2.407 1.00
151_R 166_P 2.395 1.00
124_R 156_S 2.359 1.00
457_Y 460_Q 2.358 1.00
346_M 503_A 2.357 1.00
541_R 544_E 2.341 1.00
39_L 93_R 2.324 1.00
230_H 270_E 2.312 1.00
464_A 468_Q 2.274 1.00
36_I 89_Q 2.265 1.00
478_Q 491_R 2.235 1.00
126_F 181_E 2.214 1.00
12_V 463_I 2.183 1.00
39_L 90_A 2.176 1.00
71_T 184_K 2.152 1.00
85_E 89_Q 2.15 1.00
35_V 86_L 2.047 1.00
503_A 545_A 2.045 1.00
106_T 171_L 2.023 1.00
186_C 236_M 2.011 1.00
117_L 153_H 2 1.00
81_D 84_D 1.992 1.00
456_F 460_Q 1.988 1.00
11_G 48_D 1.957 1.00
491_R 498_T 1.951 1.00
61_V 167_E 1.948 1.00
469_E 549_L 1.931 1.00
109_Q 167_E 1.913 1.00
420_S 446_E 1.909 1.00
180_A 184_K 1.888 1.00
478_Q 493_E 1.864 1.00
190_A 246_L 1.849 1.00
55_F 99_L 1.845 1.00
123_Y 126_F 1.845 1.00
152_W 157_E 1.842 1.00
491_R 500_L 1.825 1.00
83_F 99_L 1.817 1.00
497_Q 549_L 1.817 1.00
55_F 73_I 1.813 1.00
191_D 244_R 1.798 1.00
111_A 115_E 1.797 1.00
72_A 80_L 1.77 1.00
36_I 85_E 1.763 1.00
352_H 463_I 1.759 1.00
10_N 474_W 1.751 1.00
480_L 500_L 1.744 1.00
44_K 449_L 1.742 1.00
188_F 192_R 1.718 1.00
511_P 539_N 1.691 1.00
35_V 52_L 1.691 1.00
13_I 49_A 1.69 1.00
421_N 446_E 1.686 1.00
241_F 272_S 1.678 1.00
103_F 182_L 1.669 1.00
61_V 165_A 1.669 1.00
493_E 498_T 1.663 1.00
10_N 473_T 1.646 1.00
330_V 344_A 1.645 1.00
180_A 239_D 1.643 1.00
44_K 456_F 1.638 1.00
57_V 75_P 1.638 1.00
489_C 502_I 1.637 1.00
55_F 83_F 1.617 1.00
181_E 184_K 1.612 1.00
445_V 456_F 1.605 1.00
523_L 546_V 1.601 1.00
71_T 185_V 1.598 1.00
42_L 50_I 1.587 1.00
490_Y 501_V 1.585 1.00
187_E 191_D 1.582 1.00
48_D 467_K 1.567 1.00
18_P 52_L 1.557 1.00
73_I 83_F 1.547 1.00
380_R 434_I 1.539 1.00
87_V 97_I 1.534 1.00
71_T 181_E 1.534 1.00
115_E 121_S 1.525 1.00
103_F 199_L 1.523 1.00
88_T 92_S 1.514 1.00
353_G 501_V 1.497 1.00
486_V 506_S 1.484 1.00
174_E 228_R 1.473 1.00
116_A 156_S 1.467 1.00
124_R 153_H 1.467 1.00
56_Y 99_L 1.462 1.00
52_L 55_F 1.461 1.00
181_E 185_V 1.455 1.00
523_L 548_W 1.453 1.00
469_E 497_Q 1.444 1.00
83_F 194_V 1.423 1.00
511_P 537_A 1.423 1.00
234_H 270_E 1.421 1.00
153_H 156_S 1.415 1.00
42_L 95_I 1.412 1.00
33_R 37_Q 1.411 1.00
116_A 129_W 1.404 0.99
187_E 243_P 1.401 0.99
350_V 503_A 1.399 0.99
368_M 418_Q 1.395 0.99
103_F 185_V 1.386 0.99
125_Q 175_N 1.379 0.99
61_V 109_Q 1.377 0.99
108_T 167_E 1.37 0.99
345_K 458_T 1.37 0.99
82_D 85_E 1.35 0.99
424_N 430_G 1.344 0.99
487_L 540_L 1.338 0.99
384_S 405_L 1.335 0.99
378_D 435_G 1.333 0.99
71_T 188_F 1.322 0.99
183_K 235_E 1.314 0.99
103_F 186_C 1.312 0.99
476_N 493_E 1.311 0.99
346_M 505_L 1.297 0.99
15_Q 53_T 1.291 0.99
493_E 496_G 1.284 0.99
133_E 213_E 1.269 0.99
60_Q 74_D 1.268 0.99
86_L 90_A 1.267 0.99
417_M 445_V 1.263 0.99
469_E 499_L 1.261 0.99
38_H 41_Y 1.261 0.99
231_E 234_H 1.26 0.99
115_E 123_Y 1.259 0.99
36_I 40_D 1.259 0.99
369_T 439_N 1.256 0.99
187_E 239_D 1.253 0.99
57_V 74_D 1.247 0.99
114_R 117_L 1.24 0.98
11_G 49_A 1.239 0.98
501_V 547_W 1.238 0.98
353_G 472_L 1.236 0.98
47_V 95_I 1.235 0.98
461_K 464_A 1.224 0.98
523_L 526_H 1.224 0.98
18_P 35_V 1.222 0.98
460_Q 464_A 1.221 0.98
80_L 84_D 1.212 0.98
57_V 69_N 1.207 0.98
506_S 510_Q 1.204 0.98
316_W 357_T 1.2 0.98
374_T 378_D 1.188 0.98
187_E 240_V 1.188 0.98
339_Y 342_P 1.184 0.98
424_N 428_T 1.182 0.98
465_L 524_V 1.181 0.98
450_A 457_Y 1.18 0.98
449_L 456_F 1.173 0.98
201_V 204_L 1.173 0.98
349_M 462_L 1.17 0.97
237_N 241_F 1.165 0.97
228_R 231_E 1.161 0.97
502_I 546_V 1.16 0.97
101_M 186_C 1.159 0.97
122_P 177_A 1.154 0.97
398_A 402_L 1.153 0.97
190_A 240_V 1.151 0.97
321_W 347_L 1.15 0.97
322_C 329_I 1.147 0.97
366_I 418_Q 1.136 0.97
36_I 86_L 1.13 0.97
173_W 182_L 1.127 0.97
379_Y 406_A 1.124 0.97
445_V 449_L 1.123 0.97
228_R 232_F 1.122 0.97
499_L 524_V 1.119 0.96
249_V 359_Y 1.118 0.96
250_G 271_L 1.111 0.96
83_F 86_L 1.11 0.96
98_I 196_G 1.109 0.96
29_T 76_T 1.104 0.96
115_E 118_N 1.104 0.96
376_I 388_F 1.103 0.96
474_W 494_W 1.103 0.96
458_T 545_A 1.102 0.96
528_Y 541_R 1.098 0.96
179_R 183_K 1.098 0.96
471_I 499_L 1.093 0.96
472_L 499_L 1.092 0.96
88_T 91_K 1.091 0.96
345_K 454_S 1.088 0.96
353_G 503_A 1.084 0.96
392_R 401_L 1.083 0.96
449_L 457_Y 1.078 0.95
471_I 497_Q 1.074 0.95
39_L 95_I 1.074 0.95
32_L 73_I 1.073 0.95
465_L 469_E 1.071 0.95
448_A 457_Y 1.065 0.95
69_N 72_A 1.062 0.95
507_R 543_F 1.06 0.95
341_V 345_K 1.06 0.95
26_G 429_A 1.056 0.95
16_I 21_F 1.054 0.95
509_I 541_R 1.049 0.94
15_Q 18_P 1.047 0.94
43_H 95_I 1.046 0.94
32_L 86_L 1.042 0.94
484_S 510_Q 1.041 0.94
111_A 114_R 1.041 0.94
353_G 462_L 1.039 0.94
49_A 95_I 1.033 0.94
31_D 34_G 1.033 0.94
449_L 460_Q 1.033 0.94
171_L 178_V 1.032 0.94
47_V 50_I 1.03 0.94
121_S 124_R 1.03 0.94
106_T 178_V 1.023 0.93
465_L 547_W 1.022 0.93
362_Q 417_M 1.022 0.93
513_Q 537_A 1.019 0.93
486_V 510_Q 1.015 0.93
275_F 359_Y 1.01 0.93
509_I 539_N 1.009 0.93
90_A 93_R 1.008 0.93
380_R 385_L 1.007 0.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2ze0A10.98911000.11Contact Map0.858
1uokA10.99641000.111Contact Map0.857
4mb1A10.99091000.113Contact Map0.846
4hphA10.99821000.115Contact Map0.85
1m53A111000.115Contact Map0.836
1zjaA20.99641000.127Contact Map0.819
4aieA10.97461000.131Contact Map0.842
2zicA10.96371000.142Contact Map0.809
3aj7A111000.145Contact Map0.793
3zo9A20.90561000.222Contact Map0.827
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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