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OPENSEQ.org
DNLJ - DNA ligase
UniProt: P15042 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10534
Length: 671 (666)
Sequences: 1992
Seq/Len: 2.99
DNLJ
Paralog alert: 0.17 [within 20: 0.00] - ratio of genomes with paralogs
Cluster includes: DNLJ LIGB
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
7_Q 41_E 5.874 1.00
621_L 626_A 4.99 1.00
325_R 344_E 4.379 1.00
661_D 664_E 4.325 1.00
18_H 30_I 4.153 1.00
475_F 503_A 3.897 1.00
526_A 622_V 3.728 1.00
109_T 292_N 3.447 1.00
363_E 366_R 3.426 1.00
95_N 110_W 3.42 1.00
133_A 149_V 3.379 1.00
17_H 21_L 3.193 1.00
21_L 29_E 3.134 1.00
327_V 402_V 3.027 1.00
601_K 637_D 3.003 1.00
336_A 423_V 2.952 1.00
20_Y 25_M 2.93 1.00
17_H 29_E 2.852 1.00
337_I 415_V 2.849 1.00
449_M 475_F 2.82 1.00
258_T 272_K 2.817 1.00
396_P 399_T 2.816 1.00
542_S 545_E 2.627 1.00
18_H 21_L 2.563 1.00
8_L 45_L 2.547 1.00
130_L 149_V 2.511 1.00
323_F 344_E 2.475 1.00
85_V 311_V 2.461 1.00
326_D 371_I 2.452 1.00
323_F 374_K 2.442 1.00
476_K 575_E 2.409 1.00
319_E 378_R 2.389 1.00
478_T 481_K 2.373 1.00
351_V 379_R 2.361 1.00
238_T 292_N 2.346 1.00
95_N 99_Q 2.266 1.00
20_Y 24_V 2.237 1.00
536_E 577_L 2.229 1.00
638_L 660_I 2.223 1.00
129_V 158_K 2.217 1.00
351_V 383_V 2.211 1.00
15_L 19_E 2.19 1.00
645_A 649_L 2.181 1.00
501_E 504_K 2.181 1.00
494_Q 498_N 2.172 1.00
328_E 342_R 2.154 1.00
272_K 276_D 2.154 1.00
254_S 282_F 2.12 1.00
7_Q 10_E 2.108 1.00
603_V 638_L 2.092 1.00
605_L 621_L 2.079 1.00
178_Q 283_D 2.072 1.00
262_S 265_E 2.048 1.00
329_F 402_V 2.04 1.00
342_R 355_N 2.032 1.00
641_A 659_V 2.018 1.00
379_R 383_V 2.005 1.00
196_F 202_A 2.003 1.00
260_C 265_E 1.993 1.00
361_A 365_E 1.983 1.00
14_T 18_H 1.973 1.00
650_A 654_E 1.967 1.00
179_A 281_G 1.966 1.00
535_L 583_W 1.965 1.00
649_L 653_Q 1.963 1.00
641_A 649_L 1.958 1.00
610_S 643_E 1.955 1.00
44_E 48_K 1.948 1.00
330_Q 340_V 1.941 0.99
413_S 427_T 1.937 0.99
539_E 570_R 1.903 0.99
650_A 653_Q 1.903 0.99
53_I 60_Q 1.897 0.99
240_L 259_L 1.89 0.99
121_V 145_I 1.886 0.99
641_A 645_A 1.872 0.99
4_I 49_H 1.871 0.99
259_L 292_N 1.865 0.99
177_P 180_G 1.864 0.99
12_R 54_T 1.845 0.99
449_M 503_A 1.831 0.99
440_L 459_I 1.83 0.99
8_L 52_L 1.828 0.99
470_T 575_E 1.824 0.99
126_E 131_V 1.823 0.99
8_L 12_R 1.806 0.99
96_K 100_D 1.805 0.99
18_H 38_L 1.803 0.99
206_S 215_T 1.796 0.99
82_L 310_A 1.774 0.99
245_Q 249_W 1.75 0.99
604_V 636_T 1.747 0.99
378_R 386_Q 1.747 0.99
479_A 494_Q 1.746 0.99
539_E 574_S 1.737 0.99
329_F 337_I 1.733 0.99
479_A 497_V 1.733 0.99
370_R 399_T 1.726 0.99
197_A 347_H 1.724 0.99
43_R 59_T 1.723 0.99
31_P 34_E 1.709 0.98
221_T 252_P 1.7 0.98
121_V 149_V 1.699 0.98
176_L 184_I 1.693 0.98
10_E 14_T 1.691 0.98
343_L 375_V 1.69 0.98
615_D 618_K 1.686 0.98
92_L 264_E 1.676 0.98
377_I 387_V 1.674 0.98
476_K 579_E 1.659 0.98
71_S 144_D 1.652 0.98
543_I 558_S 1.637 0.98
447_R 522_E 1.621 0.98
116_L 175_F 1.618 0.98
519_E 559_H 1.616 0.97
123_I 155_I 1.602 0.97
126_E 167_R 1.6 0.97
61_R 157_L 1.599 0.97
72_Q 450_D 1.587 0.97
618_K 622_V 1.577 0.97
663_A 667_R 1.574 0.97
524_T 556_V 1.571 0.97
21_L 25_M 1.568 0.97
574_S 578_A 1.568 0.97
438_E 441_K 1.564 0.97
605_L 617_A 1.554 0.97
18_H 29_E 1.539 0.96
180_G 219_P 1.538 0.96
21_L 30_I 1.538 0.96
154_A 221_T 1.529 0.96
284_I 382_D 1.522 0.96
189_R 195_V 1.522 0.96
39_M 43_R 1.518 0.96
437_K 460_D 1.512 0.96
376_V 389_N 1.509 0.96
73_I 132_S 1.509 0.96
240_L 292_N 1.507 0.96
16_R 19_E 1.501 0.96
620_R 624_L 1.5 0.96
8_L 59_T 1.495 0.96
266_V 289_I 1.492 0.95
88_E 271_H 1.491 0.95
374_K 394_E 1.491 0.95
83_D 306_A 1.48 0.95
85_V 90_S 1.471 0.95
605_L 639_V 1.47 0.95
605_L 665_M 1.468 0.95
442_H 513_Y 1.464 0.95
151_T 216_A 1.461 0.95
527_G 549_V 1.453 0.95
88_E 267_L 1.45 0.94
40_R 90_S 1.448 0.94
407_H 414_D 1.444 0.94
276_D 279_T 1.433 0.94
149_V 174_V 1.433 0.94
237_D 241_G 1.432 0.94
361_A 404_F 1.432 0.94
602_T 627_K 1.421 0.94
370_R 401_E 1.418 0.94
508_F 529_A 1.413 0.93
87_D 90_S 1.408 0.93
326_D 342_R 1.405 0.93
660_I 668_L 1.403 0.93
127_N 166_A 1.4 0.93
121_V 222_F 1.395 0.93
349_A 379_R 1.389 0.93
92_L 263_A 1.389 0.93
261_E 265_E 1.389 0.93
365_E 401_E 1.389 0.93
109_T 261_E 1.386 0.93
124_L 167_R 1.376 0.92
527_G 551_D 1.374 0.92
40_R 43_R 1.372 0.92
520_V 556_V 1.371 0.92
612_M 666_L 1.37 0.92
458_I 488_M 1.368 0.92
289_I 313_F 1.365 0.92
149_V 152_I 1.363 0.92
93_A 96_K 1.359 0.92
451_V 500_L 1.357 0.91
264_E 268_A 1.353 0.91
410_V 437_K 1.351 0.91
143_E 208_R 1.35 0.91
605_L 640_I 1.347 0.91
21_L 27_A 1.342 0.91
497_V 501_E 1.339 0.91
214_I 217_K 1.336 0.91
8_L 42_L 1.332 0.90
182_E 186_E 1.331 0.90
324_V 390_V 1.331 0.90
454_M 496_V 1.328 0.90
18_H 28_P 1.326 0.90
490_P 494_Q 1.324 0.90
95_N 263_A 1.322 0.90
321_M 394_E 1.321 0.90
367_L 386_Q 1.318 0.90
611_Q 666_L 1.315 0.90
114_L 269_F 1.313 0.90
532_F 549_V 1.312 0.90
209_Q 214_I 1.309 0.89
293_S 296_Q 1.301 0.89
448_A 513_Y 1.3 0.89
98_V 291_V 1.298 0.89
612_M 617_A 1.293 0.89
603_V 626_A 1.289 0.88
561_H 565_A 1.283 0.88
658_E 664_E 1.282 0.88
444_V 454_M 1.281 0.88
638_L 658_E 1.277 0.88
150_R 157_L 1.273 0.88
603_V 621_L 1.273 0.88
130_L 146_T 1.255 0.87
472_A 575_E 1.253 0.86
629_A 636_T 1.253 0.86
116_L 223_F 1.25 0.86
74_R 141_T 1.245 0.86
124_L 132_S 1.244 0.86
72_Q 141_T 1.243 0.86
440_L 468_V 1.242 0.86
649_L 659_V 1.235 0.85
639_V 659_V 1.235 0.85
660_I 664_E 1.229 0.85
618_K 630_G 1.228 0.85
240_L 244_L 1.227 0.85
109_T 259_L 1.226 0.85
479_A 483_T 1.224 0.85
175_F 221_T 1.224 0.85
188_A 193_G 1.222 0.85
410_V 433_G 1.221 0.85
539_E 573_I 1.215 0.84
186_E 190_R 1.209 0.84
108_V 291_V 1.198 0.83
273_V 287_V 1.193 0.83
437_K 463_V 1.189 0.82
39_M 305_R 1.188 0.82
620_R 623_E 1.187 0.82
611_Q 663_A 1.187 0.82
155_I 222_F 1.173 0.81
380_A 386_Q 1.173 0.81
176_L 180_G 1.167 0.81
609_L 617_A 1.165 0.81
435_Q 439_S 1.163 0.80
321_M 374_K 1.159 0.80
184_I 196_F 1.158 0.80
532_F 541_A 1.15 0.79
80_L 302_F 1.149 0.79
618_K 628_V 1.146 0.79
112_C 269_F 1.146 0.79
223_F 282_F 1.136 0.78
278_P 318_Q 1.135 0.78
631_S 636_T 1.132 0.78
180_G 183_K 1.131 0.78
187_D 217_K 1.131 0.78
449_M 506_T 1.13 0.78
543_I 561_H 1.12 0.77
491_K 495_N 1.116 0.76
403_V 406_T 1.116 0.76
14_T 17_H 1.116 0.76
315_F 382_D 1.111 0.76
601_K 658_E 1.111 0.76
508_F 512_L 1.111 0.76
324_V 389_N 1.106 0.76
130_L 155_I 1.105 0.75
444_V 496_V 1.102 0.75
45_L 52_L 1.101 0.75
574_S 577_L 1.1 0.75
66_P 212_P 1.097 0.75
340_V 355_N 1.097 0.75
130_L 133_A 1.093 0.74
247_K 257_V 1.093 0.74
322_T 343_L 1.088 0.74
534_T 537_A 1.088 0.74
241_G 244_L 1.085 0.74
92_L 267_L 1.082 0.73
374_K 396_P 1.077 0.73
111_C 240_L 1.076 0.73
267_L 271_H 1.076 0.73
320_Q 348_V 1.076 0.73
611_Q 662_E 1.074 0.72
616_D 619_A 1.072 0.72
410_V 434_A 1.072 0.72
575_E 579_E 1.071 0.72
345_P 352_L 1.071 0.72
329_F 404_F 1.07 0.72
244_L 247_K 1.069 0.72
421_E 518_R 1.069 0.72
601_K 638_L 1.069 0.72
641_A 644_A 1.069 0.72
545_E 548_K 1.069 0.72
19_E 39_M 1.068 0.72
114_L 273_V 1.065 0.72
376_V 391_V 1.064 0.71
54_T 60_Q 1.064 0.71
563_F 569_N 1.062 0.71
474_L 500_L 1.061 0.71
14_T 30_I 1.06 0.71
546_L 560_V 1.06 0.71
19_E 61_R 1.059 0.71
333_R 516_G 1.058 0.71
602_T 629_A 1.058 0.71
326_D 400_R 1.057 0.71
133_A 155_I 1.056 0.71
415_V 424_A 1.052 0.70
183_K 187_D 1.051 0.70
149_V 155_I 1.051 0.70
30_I 38_L 1.051 0.70
291_V 300_L 1.05 0.70
508_F 538_L 1.048 0.70
619_A 623_E 1.045 0.69
132_S 144_D 1.043 0.69
461_Q 485_L 1.04 0.69
466_E 469_H 1.036 0.69
183_K 186_E 1.036 0.69
242_R 294_L 1.034 0.68
373_D 395_R 1.019 0.67
39_M 59_T 1.017 0.66
461_Q 487_R 1.017 0.66
487_R 493_A 1.016 0.66
425_R 518_R 1.015 0.66
642_G 646_G 1.015 0.66
474_L 493_A 1.014 0.66
271_H 274_E 1.014 0.66
111_C 239_H 1.01 0.66
260_C 266_V 1.007 0.65
543_I 547_Q 1.007 0.65
597_P 624_L 1.007 0.65
404_F 424_A 1.003 0.65
170_V 246_F 1.003 0.65
462_L 482_L 1.002 0.65
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2owoA111000.02Contact Map0.747
1dgsA20.97471000.049Contact Map0.747
4glxA10.87331000.134Contact Map0.716
3sgiA10.88671000.14Contact Map0.611
3uq8A10.47841000.525Contact Map0.766
1zauA10.46941000.534Contact Map0.513
3jslA20.47391000.535Contact Map0.826
4glwA20.45451000.54Contact Map0.714
1b04A20.47091000.545Contact Map0.811
1ta8A10.48441000.549Contact Map0.817
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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