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OPENSEQ.org
GYRB - DNA gyrase subunit B
UniProt: P0AES6 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10424
Length: 804 (792)
Sequences: 1945
Seq/Len: 2.46
GYRB
Paralog alert: 0.41 [within 20: 0.01] - ratio of genomes with paralogs
Cluster includes: GYRB PARE
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
57_K 74_D 6.083 1.00
43_V 120_V 4.683 1.00
171_P 182_F 4.241 1.00
139_K 156_V 3.791 1.00
128_Q 170_W 3.698 1.00
756_D 759_S 3.465 1.00
64_H 70_S 3.419 1.00
131_E 144_I 3.266 1.00
488_L 526_H 3.25 1.00
48_I 201_V 3.208 1.00
412_E 417_L 3.139 1.00
91_A 132_L 3.081 1.00
80_T 141_H 3.041 1.00
799_A 802_I 2.98 1.00
91_A 154_L 2.939 1.00
129_K 170_W 2.938 1.00
95_M 167_V 2.891 1.00
7_S 81_G 2.87 1.00
491_H 526_H 2.836 1.00
146_E 151_Q 2.81 1.00
105_D 108_S 2.789 1.00
135_Q 162_K 2.775 1.00
86_E 93_V 2.76 1.00
132_L 167_V 2.698 1.00
470_L 474_L 2.651 1.00
206_R 213_E 2.644 1.00
40_V 187_L 2.622 1.00
64_H 68_S 2.578 1.00
531_Q 763_L 2.541 1.00
63_I 69_V 2.538 1.00
143_Q 150_P 2.504 1.00
58_E 74_D 2.5 1.00
415_P 491_H 2.5 1.00
64_H 208_K 2.497 1.00
91_A 134_I 2.494 1.00
7_S 90_A 2.484 1.00
61_V 191_L 2.475 1.00
14_K 85_E 2.463 1.00
535_Y 549_D 2.457 1.00
152_A 155_A 2.435 1.00
64_H 168_R 2.429 1.00
129_K 144_I 2.397 1.00
195_S 218_Y 2.368 1.00
72_Q 166_M 2.351 1.00
414_D 417_L 2.35 1.00
141_H 156_V 2.316 1.00
88_V 92_E 2.3 1.00
145_Y 150_P 2.289 1.00
394_R 397_A 2.268 1.00
230_N 257_Q 2.251 1.00
70_S 168_R 2.236 1.00
474_L 488_L 2.232 1.00
296_Y 368_A 2.221 1.00
420_L 474_L 2.21 1.00
411_Q 438_R 2.188 1.00
268_C 282_L 2.181 1.00
199_S 244_S 2.179 1.00
250_I 348_A 2.174 1.00
131_E 142_R 2.162 1.00
277_D 338_D 2.142 1.00
298_D 309_S 2.14 1.00
202_S 215_H 2.127 1.00
431_S 747_P 2.117 1.00
142_R 155_A 2.104 1.00
293_L 361_L 2.099 1.00
292_T 354_N 2.098 1.00
521_I 527_V 2.092 1.00
538_K 543_E 2.089 1.00
135_Q 140_I 2.056 1.00
270_T 327_V 2.049 1.00
78_I 94_I 2.049 1.00
41_F 187_L 2.044 0.99
58_E 202_S 2.014 0.99
131_E 168_R 2.006 0.99
795_N 798_K 1.956 0.99
196_F 228_Y 1.94 0.99
529_I 765_V 1.928 0.99
422_L 466_E 1.926 0.99
18_A 99_H 1.922 0.99
134_I 154_L 1.9 0.99
447_K 466_E 1.886 0.99
268_C 275_Q 1.88 0.99
285_F 323_V 1.879 0.99
56_C 74_D 1.876 0.99
128_Q 173_L 1.863 0.99
133_V 166_M 1.861 0.99
422_L 470_L 1.841 0.99
147_H 174_E 1.84 0.99
297_M 303_S 1.839 0.99
327_V 345_V 1.827 0.99
44_V 191_L 1.825 0.99
254_V 323_V 1.815 0.99
790_A 794_E 1.813 0.98
434_Q 747_P 1.784 0.98
772_A 775_Q 1.78 0.98
310_A 375_I 1.777 0.98
48_I 194_L 1.756 0.98
147_H 175_T 1.75 0.98
65_A 208_K 1.749 0.98
447_K 450_I 1.748 0.98
520_E 524_R 1.747 0.98
56_C 59_I 1.747 0.98
139_K 159_E 1.743 0.98
357_L 361_L 1.74 0.98
133_V 142_R 1.739 0.98
184_Y 205_L 1.735 0.98
245_T 352_Q 1.726 0.98
346_K 350_E 1.698 0.97
133_V 168_R 1.697 0.97
145_Y 154_L 1.697 0.97
450_I 466_E 1.679 0.97
60_I 72_Q 1.678 0.97
224_A 227_E 1.672 0.97
192_R 228_Y 1.672 0.97
494_I 762_M 1.67 0.97
67_N 182_F 1.661 0.97
317_E 378_A 1.638 0.97
81_G 137_E 1.633 0.97
133_V 140_I 1.632 0.97
40_V 69_V 1.617 0.96
408_A 433_K 1.613 0.96
277_D 334_S 1.609 0.96
69_V 182_F 1.608 0.96
44_V 71_V 1.604 0.96
184_Y 214_D 1.602 0.96
184_Y 216_F 1.593 0.96
312_G 316_R 1.587 0.96
202_S 217_H 1.584 0.96
60_I 206_R 1.578 0.96
276_R 338_D 1.57 0.95
262_F 393_R 1.55 0.95
295_A 299_K 1.549 0.95
488_L 524_R 1.543 0.95
333_S 338_D 1.539 0.95
520_E 523_E 1.52 0.94
81_G 90_A 1.518 0.94
242_Y 253_E 1.516 0.94
14_K 86_E 1.512 0.94
394_R 409_D 1.511 0.94
11_K 105_D 1.51 0.94
418_S 491_H 1.499 0.94
95_M 120_V 1.496 0.94
474_L 493_I 1.495 0.94
334_S 341_V 1.494 0.94
88_V 97_V 1.492 0.94
270_T 275_Q 1.49 0.93
298_D 305_K 1.489 0.93
345_V 348_A 1.484 0.93
546_I 555_Y 1.481 0.93
450_I 469_T 1.479 0.93
514_F 527_V 1.478 0.93
8_S 84_P 1.476 0.93
63_I 67_N 1.474 0.93
290_T 312_G 1.469 0.93
234_T 260_D 1.469 0.93
364_N 367_D 1.466 0.93
11_K 14_K 1.456 0.92
285_F 321_A 1.453 0.92
275_Q 334_S 1.447 0.92
246_E 251_G 1.445 0.92
420_L 470_L 1.441 0.92
16_L 19_V 1.44 0.92
416_A 491_H 1.438 0.92
10_I 90_A 1.429 0.91
519_P 523_E 1.425 0.91
250_I 345_V 1.424 0.91
62_T 72_Q 1.415 0.91
303_S 307_K 1.413 0.91
192_R 196_F 1.413 0.91
144_I 151_Q 1.41 0.91
466_E 469_T 1.405 0.91
130_L 167_V 1.396 0.90
275_Q 341_V 1.395 0.90
194_L 198_N 1.391 0.90
156_V 159_E 1.389 0.90
450_I 467_V 1.388 0.90
140_I 160_T 1.388 0.90
287_A 350_E 1.386 0.90
140_I 157_T 1.384 0.90
230_N 320_I 1.366 0.89
421_Y 762_M 1.363 0.89
422_L 446_L 1.358 0.88
16_L 98_L 1.357 0.88
60_I 204_R 1.353 0.88
434_Q 746_N 1.351 0.88
333_S 341_V 1.346 0.88
120_V 130_L 1.336 0.87
51_A 201_V 1.324 0.87
144_I 152_A 1.319 0.86
484_N 487_K 1.312 0.86
466_E 497_T 1.309 0.86
80_T 136_R 1.305 0.86
93_V 97_V 1.302 0.85
410_C 489_R 1.299 0.85
404_P 409_D 1.296 0.85
129_K 146_E 1.293 0.85
789_R 793_E 1.292 0.85
135_Q 166_M 1.29 0.85
450_I 497_T 1.29 0.85
256_L 319_L 1.287 0.85
485_P 524_R 1.283 0.84
11_K 21_K 1.281 0.84
140_I 158_G 1.274 0.84
374_K 393_R 1.274 0.84
81_G 470_L 1.273 0.84
537_V 552_M 1.272 0.84
68_S 171_P 1.267 0.83
791_F 796_A 1.255 0.82
62_T 208_K 1.253 0.82
268_C 334_S 1.241 0.82
262_F 374_K 1.238 0.81
305_K 309_S 1.238 0.81
394_R 398_L 1.237 0.81
495_I 514_F 1.237 0.81
18_A 21_K 1.233 0.81
748_E 756_D 1.229 0.81
266_I 286_R 1.226 0.80
24_G 30_T 1.224 0.80
770_A 773_A 1.222 0.80
192_R 218_Y 1.222 0.80
47_A 167_V 1.217 0.80
739_Y 749_Q 1.213 0.79
404_P 489_R 1.213 0.79
9_S 108_S 1.212 0.79
43_V 95_M 1.202 0.79
775_Q 779_T 1.201 0.78
172_S 175_T 1.198 0.78
40_V 44_V 1.189 0.78
314_D 382_R 1.188 0.77
58_E 204_R 1.188 0.77
283_A 287_A 1.188 0.77
270_T 341_V 1.185 0.77
70_S 166_M 1.184 0.77
415_P 418_S 1.184 0.77
381_A 388_A 1.18 0.77
231_K 775_Q 1.176 0.76
409_D 489_R 1.171 0.76
246_E 249_G 1.171 0.76
90_A 93_V 1.17 0.76
361_L 368_A 1.169 0.76
737_Q 749_Q 1.168 0.76
467_V 497_T 1.168 0.76
18_A 24_G 1.165 0.75
548_D 551_A 1.165 0.75
136_R 163_T 1.165 0.75
196_F 218_Y 1.164 0.75
470_L 513_F 1.161 0.75
784_A 787_P 1.159 0.75
68_S 170_W 1.156 0.75
232_N 778_T 1.155 0.75
437_N 440_N 1.154 0.75
353_M 357_L 1.151 0.74
381_A 385_A 1.151 0.74
433_K 749_Q 1.15 0.74
135_Q 163_T 1.148 0.74
507_R 529_I 1.146 0.74
546_I 552_M 1.146 0.74
81_G 136_R 1.145 0.74
384_A 388_A 1.138 0.73
63_I 209_R 1.138 0.73
356_L 359_E 1.138 0.73
19_V 98_L 1.137 0.73
342_S 345_V 1.13 0.72
476_C 485_P 1.127 0.72
496_M 755_M 1.126 0.72
331_K 341_V 1.124 0.72
169_F 182_F 1.123 0.72
270_T 334_S 1.122 0.72
381_A 384_A 1.121 0.71
44_V 61_V 1.119 0.71
293_L 315_A 1.118 0.71
400_L 468_A 1.116 0.71
222_I 242_Y 1.111 0.70
552_M 734_L 1.107 0.70
486_D 524_R 1.1 0.69
338_D 342_S 1.1 0.69
417_L 439_K 1.089 0.68
476_C 483_Y 1.089 0.68
334_S 338_D 1.088 0.68
193_E 312_G 1.085 0.68
404_P 473_A 1.085 0.68
385_A 388_A 1.084 0.68
143_Q 154_L 1.08 0.67
454_E 516_R 1.079 0.67
476_C 488_L 1.079 0.67
223_K 227_E 1.079 0.67
89_S 152_A 1.077 0.67
31_D 342_S 1.075 0.67
421_Y 755_M 1.075 0.67
228_Y 775_Q 1.071 0.66
9_S 107_N 1.071 0.66
55_H 76_R 1.07 0.66
52_L 205_L 1.068 0.66
64_H 67_N 1.065 0.66
501_V 803_D 1.064 0.66
418_S 526_H 1.064 0.66
380_R 457_R 1.062 0.65
41_F 186_I 1.061 0.65
31_D 343_S 1.059 0.65
94_I 134_I 1.052 0.64
191_L 205_L 1.05 0.64
254_V 353_M 1.048 0.64
250_I 327_V 1.044 0.64
216_F 471_I 1.041 0.63
377_D 393_R 1.04 0.63
504_S 749_Q 1.037 0.63
110_K 272_N 1.034 0.62
470_L 497_T 1.033 0.62
630_V 678_D 1.033 0.62
275_Q 331_K 1.032 0.62
508_T 777_F 1.031 0.62
113_G 273_I 1.031 0.62
150_P 153_P 1.03 0.62
83_H 90_A 1.028 0.62
322_V 515_Y 1.027 0.62
411_Q 746_N 1.025 0.61
602_P 675_Y 1.024 0.61
11_K 85_E 1.024 0.61
497_T 504_S 1.022 0.61
95_M 130_L 1.021 0.61
21_K 329_D 1.021 0.61
627_T 631_N 1.017 0.60
459_D 463_S 1.015 0.60
6_D 9_S 1.014 0.60
782_G 792_I 1.014 0.60
89_S 93_V 1.014 0.60
460_K 803_D 1.012 0.60
270_T 325_V 1.011 0.60
447_K 465_Q 1.01 0.60
791_F 803_D 1.01 0.60
796_A 803_D 1.009 0.60
67_N 181_E 1.007 0.59
97_V 145_Y 1.007 0.59
31_D 338_D 1.007 0.59
294_N 298_D 1.006 0.59
405_G 473_A 1.005 0.59
409_D 418_S 1.004 0.59
252_V 348_A 1.004 0.59
195_S 375_I 1.001 0.59
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
4i3hA20.77861000.253Contact Map0.598
4gfhA20.75751000.31Contact Map0.49
3nuhB10.52241000.589Contact Map0.623
3zkbA160.48261000.602Contact Map0.808
1ei1A20.48631000.627Contact Map0.785
3lnuA10.48011000.633Contact Map0.661
1s16A20.48131000.641Contact Map0.714
1kijA20.48261000.646Contact Map0.713
1pvgA20.46021000.647Contact Map0.556
1zxmA20.45771000.648Contact Map0.536
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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