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OPENSEQ.org
CISY - Citrate synthase
UniProt: P0ABH7 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10402
Length: 427 (425)
Sequences: 1963
Seq/Len: 4.62
CISY
Paralog alert: 0.53 [within 20: 0.03] - ratio of genomes with paralogs
Cluster includes: CISY PRPC
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
242_A 255_A 5.797 1.00
76_Q 420_D 4.357 1.00
98_Q 102_D 3.814 1.00
239_V 256_G 3.628 1.00
73_P 76_Q 3.411 1.00
94_E 100_Q 3.188 1.00
196_G 217_E 2.892 1.00
342_E 345_N 2.868 1.00
284_H 287_E 2.75 1.00
81_S 421_F 2.674 1.00
193_S 196_G 2.634 1.00
324_H 341_M 2.524 1.00
286_P 342_E 2.516 1.00
213_N 329_E 2.505 1.00
79_T 321_E 2.498 1.00
345_N 349_N 2.45 1.00
94_E 419_R 2.381 1.00
292_A 299_F 2.296 1.00
245_S 259_S 2.259 1.00
193_S 217_E 2.194 1.00
238_T 259_S 2.156 1.00
292_A 297_D 2.153 1.00
216_L 373_M 2.147 1.00
291_R 295_K 2.133 1.00
82_N 101_Y 2.121 1.00
151_D 154_N 2.112 1.00
120_R 123_H 2.112 1.00
241_T 407_A 2.097 1.00
48_T 405_K 2.093 1.00
351_P 355_E 2.081 1.00
234_A 245_S 2.067 1.00
138_I 172_M 2.036 1.00
97_T 100_Q 2.032 1.00
242_A 259_S 2.016 1.00
154_N 157_H 2.011 1.00
22_K 28_D 1.976 1.00
277_E 371_K 1.967 1.00
98_Q 425_I 1.959 1.00
270_E 274_K 1.855 1.00
321_E 325_E 1.837 1.00
76_Q 80_D 1.817 1.00
280_S 284_H 1.768 1.00
197_N 201_M 1.755 1.00
99_E 102_D 1.754 1.00
202_M 375_I 1.748 1.00
320_R 324_H 1.744 1.00
139_T 257_I 1.737 1.00
108_V 163_F 1.688 0.99
88_Y 94_E 1.677 0.99
72_F 419_R 1.674 0.99
192_L 196_G 1.654 0.99
247_A 261_W 1.649 0.99
186_V 204_S 1.637 0.99
215_I 329_E 1.636 0.99
424_D 427_R 1.614 0.99
107_T 163_F 1.603 0.99
99_E 103_E 1.593 0.99
287_E 291_R 1.592 0.99
340_A 368_I 1.585 0.99
375_I 383_I 1.579 0.99
137_G 140_G 1.565 0.99
342_E 346_I 1.557 0.99
223_I 370_L 1.555 0.99
218_R 325_E 1.552 0.99
324_H 328_K 1.551 0.99
102_D 106_T 1.544 0.99
162_A 393_I 1.54 0.99
173_A 382_V 1.528 0.98
178_K 183_Q 1.503 0.98
85_E 101_Y 1.498 0.98
173_A 386_M 1.495 0.98
136_C 258_A 1.495 0.98
91_L 166_L 1.494 0.98
171_T 188_P 1.485 0.98
189_R 200_N 1.483 0.98
103_E 107_T 1.446 0.98
72_F 95_K 1.44 0.98
234_A 259_S 1.422 0.97
7_K 15_A 1.411 0.97
116_E 120_R 1.407 0.97
84_L 108_V 1.361 0.96
218_R 221_D 1.355 0.96
136_C 140_G 1.341 0.96
120_R 124_A 1.34 0.96
218_R 222_R 1.339 0.96
92_N 159_E 1.335 0.96
195_A 221_D 1.324 0.95
102_D 105_K 1.32 0.95
291_R 297_D 1.307 0.95
9_T 15_A 1.306 0.95
14_T 37_S 1.3 0.95
169_M 389_T 1.29 0.94
321_E 324_H 1.287 0.94
106_T 110_R 1.285 0.94
290_R 294_D 1.275 0.94
121_L 141_A 1.272 0.94
87_C 390_V 1.272 0.94
277_E 377_S 1.265 0.94
423_S 426_K 1.26 0.93
139_T 169_M 1.26 0.93
387_A 390_V 1.259 0.93
323_C 368_I 1.255 0.93
334_D 371_K 1.254 0.93
130_H 270_E 1.252 0.93
85_E 421_F 1.247 0.93
89_I 95_K 1.247 0.93
75_D 317_T 1.244 0.93
286_P 351_P 1.237 0.93
291_R 294_D 1.231 0.92
114_I 175_M 1.229 0.92
132_M 269_N 1.219 0.92
69_H 228_A 1.218 0.92
214_P 218_R 1.206 0.91
289_V 347_A 1.205 0.91
121_L 124_A 1.204 0.91
34_T 37_S 1.202 0.91
86_V 225_I 1.201 0.91
228_A 390_V 1.199 0.91
282_V 345_N 1.198 0.91
61_D 64_E 1.194 0.91
292_A 298_S 1.193 0.91
223_I 319_M 1.191 0.91
174_A 198_F 1.19 0.91
62_G 65_G 1.189 0.91
136_C 254_A 1.188 0.91
324_H 337_L 1.188 0.91
48_T 307_R 1.179 0.90
11_N 14_T 1.175 0.90
119_T 175_M 1.168 0.90
100_Q 103_E 1.161 0.89
111_H 164_R 1.157 0.89
238_T 242_A 1.156 0.89
227_H 362_V 1.153 0.89
282_V 342_E 1.149 0.89
282_V 339_V 1.149 0.89
80_D 423_S 1.141 0.88
189_R 211_E 1.139 0.88
213_N 216_L 1.129 0.87
192_L 197_N 1.127 0.87
255_A 259_S 1.123 0.87
112_T 190_N 1.121 0.87
212_V 217_E 1.121 0.87
288_F 292_A 1.118 0.87
73_P 420_D 1.117 0.87
115_H 126_R 1.114 0.86
290_R 355_E 1.11 0.86
81_S 225_I 1.11 0.86
58_T 229_D 1.108 0.86
192_L 217_E 1.107 0.86
140_G 258_A 1.103 0.86
94_E 416_Y 1.102 0.86
125_F 144_A 1.102 0.86
284_H 291_R 1.1 0.85
8_L 18_L 1.096 0.85
272_A 339_V 1.086 0.84
134_V 176_C 1.082 0.84
156_R 160_I 1.082 0.84
107_T 160_I 1.08 0.84
23_G 27_Q 1.072 0.83
246_G 264_A 1.072 0.83
157_H 161_A 1.071 0.83
30_I 35_L 1.068 0.83
243_G 252_C 1.062 0.83
312_Y 317_T 1.06 0.82
269_N 363_D 1.06 0.82
21_L 28_D 1.059 0.82
286_P 346_I 1.057 0.82
180_S 378_S 1.056 0.82
291_R 296_N 1.056 0.82
75_D 318_V 1.055 0.82
289_V 346_I 1.053 0.82
16_V 38_K 1.041 0.81
195_A 220_M 1.038 0.81
253_I 393_I 1.037 0.81
135_M 173_A 1.037 0.81
145_F 154_N 1.034 0.80
12_G 15_A 1.028 0.80
112_T 194_Y 1.019 0.79
83_Y 87_C 1.017 0.79
196_G 214_P 1.017 0.79
117_Q 127_R 1.015 0.79
285_I 289_V 1.013 0.78
11_N 40_V 1.012 0.78
6_A 12_G 1.012 0.78
323_C 340_A 1.011 0.78
91_L 393_I 1.005 0.78
341_M 345_N 1.005 0.78
189_R 192_L 1.003 0.77
280_S 335_D 1.003 0.77
105_K 109_T 1.002 0.77
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
2h12A60.9931000.067Contact Map0.739
3l96A20.99771000.087Contact Map0.681
3msuA20.97421000.092Contact Map0.692
2ibpA20.92741000.153Contact Map0.702
1iomA10.87821000.153Contact Map0.617
1o7xA40.87821000.157Contact Map0.735
1vgpA10.86891000.16Contact Map0.632
1vgmA20.87351000.161Contact Map0.698
1aj8A20.86651000.162Contact Map0.749
3tqgA20.87351000.166Contact Map0.708
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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