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OPENSEQ.org
USHA - Protein UshA
UniProt: P07024 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG11060
Length: 550 (515)
Sequences: 2004
Seq/Len: 3.89
USHA
Paralog alert: 0.60 [within 20: 0.06] - ratio of genomes with paralogs
Cluster includes: CPDB USHA
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
444_I 487_P 3.7 1.00
39_T 109_Y 3.672 1.00
478_L 485_V 3.407 1.00
441_K 445_D 3.38 1.00
365_G 530_I 3.213 1.00
486_D 489_K 3.172 1.00
439_T 442_E 3.123 1.00
64_D 342_N 3.04 1.00
80_L 167_V 2.92 1.00
363_K 417_D 2.899 1.00
170_L 196_A 2.86 1.00
166_A 203_L 2.851 1.00
237_A 248_I 2.708 1.00
190_R 195_E 2.703 1.00
437_D 490_T 2.697 1.00
158_F 167_V 2.568 1.00
155_W 199_V 2.561 1.00
60_K 64_D 2.553 1.00
469_V 477_D 2.541 1.00
438_M 493_M 2.524 1.00
448_T 474_K 2.444 1.00
444_I 478_L 2.414 1.00
199_V 202_E 2.361 1.00
346_I 350_S 2.332 1.00
111_A 167_V 2.316 1.00
341_E 346_I 2.291 1.00
166_A 209_P 2.285 1.00
139_S 171_T 2.216 1.00
394_M 464_A 2.21 1.00
437_D 492_R 2.188 1.00
159_K 162_D 2.18 1.00
204_Q 212_I 2.164 1.00
202_E 207_E 2.156 1.00
235_E 239_A 2.115 1.00
467_S 479_K 2.103 1.00
36_V 213_I 2.084 1.00
448_T 472_D 2.079 1.00
197_K 201_Q 2.079 1.00
79_L 107_V 2.056 1.00
111_A 138_L 2.039 1.00
350_S 354_N 2.038 1.00
447_L 478_L 2.022 1.00
33_K 301_E 2.021 1.00
401_F 443_V 2.011 1.00
39_T 81_S 2.01 1.00
391_A 464_A 1.999 1.00
389_I 409_I 1.997 1.00
343_Q 347_S 1.991 1.00
479_K 484_P 1.934 1.00
157_L 209_P 1.924 1.00
163_L 211_I 1.918 1.00
438_M 443_V 1.912 1.00
36_V 247_M 1.901 1.00
63_V 79_L 1.896 1.00
79_L 109_Y 1.885 1.00
160_R 165_I 1.877 1.00
80_L 111_A 1.875 1.00
433_V 494_A 1.872 1.00
366_E 417_D 1.825 1.00
62_L 295_V 1.81 0.99
133_A 137_L 1.808 0.99
248_I 285_I 1.795 0.99
82_G 249_V 1.783 0.99
443_V 485_V 1.764 0.99
80_L 215_A 1.749 0.99
439_T 490_T 1.738 0.99
113_A 138_L 1.726 0.99
105_N 135_F 1.72 0.99
201_Q 205_Q 1.717 0.99
81_S 109_Y 1.71 0.99
168_I 196_A 1.695 0.99
35_T 297_R 1.683 0.99
66_I 69_E 1.676 0.99
175_T 189_F 1.676 0.99
418_I 530_I 1.67 0.99
364_I 423_V 1.659 0.99
144_Q 147_T 1.648 0.99
105_N 134_K 1.639 0.99
214_A 245_L 1.629 0.99
145_K 186_D 1.628 0.99
441_K 487_P 1.627 0.99
524_E 528_A 1.623 0.99
62_L 65_G 1.623 0.99
61_T 65_G 1.623 0.99
247_M 298_A 1.618 0.99
143_Y 190_R 1.618 0.99
40_N 288_A 1.611 0.99
446_Y 508_R 1.595 0.98
435_Y 492_R 1.556 0.98
196_A 245_L 1.556 0.98
173_D 192_P 1.55 0.98
36_V 249_V 1.542 0.98
408_G 431_N 1.531 0.98
395_D 533_S 1.525 0.98
247_M 284_W 1.506 0.97
203_L 209_P 1.502 0.97
367_T 418_I 1.49 0.97
217_H 252_H 1.49 0.97
157_L 164_K 1.471 0.97
117_H 120_D 1.468 0.97
200_I 240_L 1.455 0.97
382_Q 387_R 1.45 0.97
111_A 136_P 1.449 0.97
362_V 531_Q 1.445 0.97
190_R 198_L 1.441 0.96
38_H 296_G 1.423 0.96
471_K 474_K 1.415 0.96
361_E 421_K 1.411 0.96
404_M 408_G 1.409 0.96
441_K 488_A 1.404 0.96
33_K 299_D 1.4 0.96
125_V 128_Q 1.398 0.96
155_W 166_A 1.396 0.96
154_P 199_V 1.39 0.95
436_A 500_A 1.388 0.95
247_M 300_F 1.381 0.95
233_D 250_G 1.375 0.95
104_M 109_Y 1.363 0.95
43_H 88_G 1.361 0.95
106_L 345_M 1.358 0.95
78_L 158_F 1.357 0.95
145_K 188_E 1.35 0.94
200_I 212_I 1.349 0.94
438_M 442_E 1.344 0.94
38_H 249_V 1.343 0.94
423_V 523_A 1.333 0.94
67_R 79_L 1.331 0.94
403_V 443_V 1.315 0.93
84_D 116_N 1.293 0.93
101_F 105_N 1.287 0.92
502_G 505_G 1.285 0.92
101_F 133_A 1.284 0.92
92_S 121_N 1.282 0.92
401_F 480_I 1.282 0.92
218_M 236_M 1.279 0.92
61_T 340_A 1.276 0.92
240_L 245_L 1.262 0.91
163_L 210_D 1.261 0.91
386_G 462_Q 1.258 0.91
441_K 444_I 1.254 0.91
249_V 286_V 1.253 0.91
67_R 108_G 1.248 0.91
372_E 377_K 1.246 0.91
106_L 348_L 1.244 0.91
443_V 478_L 1.244 0.91
195_E 199_V 1.244 0.91
461_P 506_Y 1.23 0.90
253_S 287_Q 1.226 0.90
112_M 135_F 1.224 0.90
363_K 419_S 1.219 0.89
159_K 164_K 1.218 0.89
369_G 384_N 1.215 0.89
407_G 410_R 1.211 0.89
420_Y 527_K 1.211 0.89
440_G 491_Y 1.204 0.89
395_D 532_K 1.201 0.88
37_L 77_V 1.2 0.88
197_K 241_P 1.198 0.88
496_L 499_N 1.194 0.88
198_L 202_E 1.19 0.88
347_S 350_S 1.186 0.88
486_D 491_Y 1.184 0.87
414_E 422_N 1.182 0.87
143_Y 150_R 1.18 0.87
371_L 384_N 1.175 0.87
235_E 238_R 1.175 0.87
70_V 77_V 1.175 0.87
196_A 214_A 1.172 0.87
386_G 409_I 1.168 0.87
104_M 135_F 1.166 0.86
495_T 500_A 1.163 0.86
64_D 344_Q 1.162 0.86
362_V 420_Y 1.158 0.86
117_H 217_H 1.156 0.86
184_F 189_F 1.155 0.86
393_Q 402_A 1.154 0.86
38_H 82_G 1.154 0.86
143_Y 188_E 1.149 0.85
40_N 287_Q 1.146 0.85
116_N 252_H 1.145 0.85
449_A 508_R 1.141 0.85
42_H 56_L 1.139 0.85
78_L 167_V 1.137 0.84
65_G 68_K 1.136 0.84
194_D 197_K 1.133 0.84
469_V 476_N 1.13 0.84
465_N 480_I 1.129 0.84
59_Q 63_V 1.126 0.84
405_S 408_G 1.126 0.84
191_K 194_D 1.124 0.84
116_N 217_H 1.12 0.83
168_I 214_A 1.114 0.83
385_M 426_V 1.111 0.83
60_K 345_M 1.111 0.83
193_A 239_A 1.11 0.82
411_D 425_K 1.107 0.82
397_T 435_Y 1.106 0.82
204_Q 242_A 1.106 0.82
484_P 487_P 1.102 0.82
62_L 339_I 1.102 0.82
370_R 415_A 1.101 0.82
378_V 383_T 1.1 0.82
112_M 137_L 1.098 0.82
432_V 497_N 1.097 0.81
471_K 476_N 1.095 0.81
410_R 429_F 1.089 0.81
448_T 475_L 1.088 0.81
480_I 491_Y 1.088 0.81
405_S 431_N 1.087 0.81
209_P 212_I 1.085 0.80
444_I 473_G 1.084 0.80
35_T 299_D 1.081 0.80
87_T 118_E 1.076 0.80
192_P 236_M 1.073 0.79
123_L 187_I 1.072 0.79
59_Q 295_V 1.07 0.79
190_R 194_D 1.068 0.79
498_F 504_D 1.068 0.79
139_S 142_I 1.066 0.79
43_H 287_Q 1.066 0.79
168_I 199_V 1.063 0.79
357_K 361_E 1.063 0.79
43_H 219_G 1.062 0.78
101_F 129_Q 1.059 0.78
443_V 466_V 1.059 0.78
143_Y 195_E 1.053 0.78
217_H 254_Q 1.05 0.77
84_D 117_H 1.049 0.77
367_T 370_R 1.047 0.77
368_N 537_D 1.042 0.77
219_G 253_S 1.041 0.76
65_G 340_A 1.041 0.76
436_A 495_T 1.039 0.76
193_A 240_L 1.038 0.76
155_W 202_E 1.038 0.76
216_T 250_G 1.037 0.76
290_E 293_K 1.036 0.76
113_A 139_S 1.035 0.76
422_N 425_K 1.031 0.76
110_D 158_F 1.03 0.75
483_E 489_K 1.03 0.75
234_V 285_I 1.03 0.75
442_E 511_N 1.027 0.75
367_T 413_I 1.022 0.75
45_H 291_W 1.014 0.74
289_H 293_K 1.014 0.74
158_F 165_I 1.01 0.73
369_G 538_V 1.009 0.73
403_V 446_Y 1.008 0.73
388_L 392_A 1.007 0.73
437_D 514_G 1.006 0.73
116_N 120_D 1.006 0.73
430_G 523_A 1.006 0.73
41_D 254_Q 1.006 0.73
371_L 413_I 1.003 0.73
444_I 485_V 1.003 0.73
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1hp1A10.93821000.252Contact Map0.771
2z1aA10.91451000.297Contact Map0.792
4h2gA10.90911000.306Contact Map0.809
3iveA10.85641000.312Contact Map0.795
3ztvA10.91451000.32Contact Map0.764
3qfkA10.87451000.335Contact Map0.728
2wdcA10.87271000.343Contact Map0.724
3c9fA20.88551000.495Contact Map0.571
3gveA20.56361000.596Contact Map0.816
3jyfA20.56731000.604Contact Map0.812
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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