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OPENSEQ.org
AGAL - Alpha-galactosidase
UniProt: P06720 Sequence
 Download Alignment
We filter this alignment to remove sites that have > 75% gaps before running GREMLIN.
EcoGene: EG10577
Length: 451 (442)
Sequences: 715
Seq/Len: 1.62
AGAL
Paralog alert: 0.64 [within 20: 0.05] - ratio of genomes with paralogs
Cluster includes: AGAL CHBF GLVG
GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Scaled Score Prob
73_K 139_E 4.506 1.00
81_F 336_W 3.249 1.00
72_Q 136_D 3.101 1.00
76_L 141_C 3.087 1.00
107_L 117_P 2.824 1.00
345_G 368_V 2.798 1.00
412_Y 434_V 2.784 1.00
35_H 65_K 2.612 1.00
104_R 436_D 2.492 1.00
137_M 146_M 2.458 1.00
369_D 374_Q 2.423 1.00
107_L 433_L 2.342 1.00
334_A 341_S 2.314 1.00
381_L 389_M 2.304 1.00
222_L 225_E 2.299 1.00
209_E 221_N 2.261 0.99
401_E 405_T 2.252 0.99
50_H 68_C 2.251 0.99
187_L 226_L 2.223 0.99
47_E 51_I 2.185 0.99
120_I 399_L 2.139 0.99
137_M 167_I 2.105 0.99
71_Q 74_E 2.1 0.99
345_G 366_C 2.099 0.99
408_R 435_D 2.095 0.99
193_T 216_D 2.065 0.99
35_H 67_T 2.06 0.99
138_T 165_P 2.004 0.98
354_D 380_T 1.973 0.98
134_C 160_M 1.969 0.98
74_E 77_E 1.958 0.98
135_E 138_T 1.948 0.98
356_L 389_M 1.946 0.98
50_H 54_R 1.904 0.98
137_M 141_C 1.88 0.97
22_L 36_I 1.864 0.97
51_I 54_R 1.852 0.97
143_D 168_K 1.846 0.97
405_T 410_R 1.837 0.97
355_N 386_A 1.805 0.96
105_H 432_A 1.801 0.96
117_P 424_V 1.801 0.96
6_K 37_A 1.78 0.96
88_I 129_H 1.746 0.95
73_K 136_D 1.737 0.95
145_T 168_K 1.718 0.95
411_V 434_V 1.714 0.95
415_A 437_L 1.671 0.94
352_L 362_V 1.665 0.93
78_D 143_D 1.642 0.93
132_Q 135_E 1.629 0.92
120_I 395_V 1.626 0.92
101_V 440_A 1.605 0.92
92_E 96_V 1.604 0.92
43_P 47_E 1.604 0.92
214_T 217_G 1.603 0.92
42_D 90_G 1.597 0.91
204_M 392_N 1.593 0.91
162_A 401_E 1.59 0.91
338_G 370_A 1.574 0.91
340_P 369_D 1.573 0.91
386_A 390_Q 1.567 0.90
37_A 69_H 1.553 0.90
128_P 403_I 1.534 0.89
126_T 129_H 1.53 0.89
206_F 277_I 1.521 0.89
369_D 373_I 1.515 0.88
190_D 193_T 1.514 0.88
30_A 333_N 1.508 0.88
383_S 394_N 1.496 0.87
439_A 442_G 1.481 0.87
311_E 314_K 1.467 0.86
298_R 302_Q 1.465 0.86
170_V 373_I 1.463 0.86
347_V 368_V 1.437 0.84
215_A 222_L 1.435 0.84
295_Y 303_L 1.417 0.83
354_D 358_Q 1.384 0.81
115_L 392_N 1.383 0.81
38_L 78_D 1.379 0.81
228_A 232_A 1.379 0.81
13_G 40_D 1.379 0.81
8_T 79_A 1.374 0.81
96_V 299_C 1.37 0.80
37_A 67_T 1.366 0.80
160_M 169_Q 1.362 0.80
220_V 225_E 1.352 0.79
180_A 191_P 1.349 0.79
77_E 140_V 1.318 0.77
82_V 144_A 1.312 0.76
154_A 396_Q 1.308 0.76
350_D 358_Q 1.305 0.76
433_L 437_L 1.305 0.76
127_I 159_A 1.301 0.75
81_F 335_I 1.28 0.74
120_I 411_V 1.28 0.74
121_M 141_C 1.277 0.73
308_K 312_E 1.277 0.73
253_E 276_F 1.275 0.73
195_R 211_E 1.275 0.73
47_E 68_C 1.27 0.73
335_I 338_G 1.269 0.73
333_N 337_T 1.265 0.72
355_N 379_G 1.264 0.72
342_V 365_A 1.263 0.72
369_D 372_G 1.252 0.71
24_D 174_H 1.235 0.70
299_C 302_Q 1.228 0.69
395_V 415_A 1.226 0.69
410_R 413_H 1.218 0.68
354_D 377_K 1.213 0.68
9_F 83_V 1.209 0.67
47_E 50_H 1.202 0.67
161_Y 348_R 1.197 0.66
73_K 140_V 1.196 0.66
435_D 438_I 1.186 0.65
6_K 35_H 1.17 0.64
8_T 76_L 1.161 0.63
99_F 111_I 1.16 0.63
307_H 311_E 1.156 0.62
105_H 436_D 1.155 0.62
407_N 410_R 1.154 0.62
293_D 296_P 1.149 0.62
149_Y 154_A 1.145 0.61
70_T 74_E 1.143 0.61
347_V 366_C 1.14 0.61
342_V 367_L 1.14 0.61
67_T 74_E 1.14 0.61
78_D 141_C 1.139 0.61
275_W 388_L 1.135 0.60
108_E 285_I 1.134 0.60
137_M 335_I 1.125 0.59
353_I 362_V 1.121 0.59
147_L 332_M 1.118 0.59
138_T 166_H 1.116 0.58
352_L 364_V 1.114 0.58
264_E 267_E 1.114 0.58
131_W 159_A 1.114 0.58
123_A 361_C 1.113 0.58
172_L 270_A 1.113 0.58
350_D 361_C 1.104 0.57
198_C 205_A 1.102 0.57
102_C 118_G 1.102 0.57
30_A 339_E 1.099 0.57
143_D 190_D 1.097 0.56
204_M 388_L 1.096 0.56
66_I 378_V 1.095 0.56
289_K 294_E 1.094 0.56
121_M 438_I 1.083 0.55
82_V 137_M 1.08 0.55
98_D 125_R 1.08 0.55
225_E 228_A 1.08 0.55
21_I 85_A 1.08 0.55
8_T 37_A 1.077 0.54
305_N 308_K 1.074 0.54
47_E 370_A 1.071 0.54
35_H 38_L 1.071 0.54
77_E 143_D 1.07 0.54
381_L 386_A 1.067 0.53
408_R 434_V 1.061 0.53
367_L 376_T 1.061 0.53
426_G 429_E 1.058 0.52
87_Q 99_F 1.057 0.52
366_C 375_P 1.052 0.52
185_R 350_D 1.051 0.52
56_L 144_A 1.051 0.52
223_Y 227_L 1.051 0.52
126_T 403_I 1.045 0.51
10_I 146_M 1.043 0.51
44_T 307_H 1.038 0.50
164_Y 404_L 1.034 0.50
157_T 161_Y 1.031 0.49
35_H 225_E 1.028 0.49
105_H 433_L 1.026 0.49
180_A 196_Y 1.024 0.49
225_E 229_A 1.023 0.49
401_E 410_R 1.022 0.49
38_L 92_E 1.021 0.48
153_M 176_V 1.021 0.48
57_M 64_G 1.02 0.48
402_A 411_V 1.016 0.48
120_I 437_L 1.016 0.48
253_E 431_Y 1.014 0.48
338_G 364_V 1.013 0.48
18_V 85_A 1.009 0.47
262_V 269_F 1.009 0.47
66_I 183_L 1.007 0.47
117_P 415_A 1.006 0.47
7_I 31_L 1.001 0.46
333_N 339_E 1.001 0.46
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Scaled Score = (raw_score/average(raw_scores)) > 1 are listed. For all 3L/2 predictions, click on one of the links below.
HHsearch Results: GREMLIN results overlayed on top 10 PDB hits:
Legend: Blue filled circles are GREMLIN results (Scaled_score >1). The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on 10 HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov P(%) HHΔ   Acc
1obbA20.95791000.375Contact Map0.582
1u8xX10.94681000.382Contact Map0.621
1s6yA10.93571000.387Contact Map0.701
3fefA40.96231000.393Contact Map0.614
1up7A80.90691000.396Contact Map0.71
3u95A60.95121000.435Contact Map0.619
1pzgA40.71181000.728Contact Map0.571
3gviA60.70071000.731Contact Map0.591
3p7mA40.69181000.732Contact Map0.589
1t2dA10.6941000.735Contact Map0.626
Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.
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